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Protein

Interphotoreceptor matrix proteoglycan 2

Gene

Impg2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Chondroitin sulfate- and hyaluronan-binding proteoglycan involved in the organization of interphotoreceptor matrix; may participate in the maturation and maintenance of the light-sensitive photoreceptor outer segment. Binds heparin.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHeparin-binding, Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Interphotoreceptor matrix proteoglycan 2
Alternative name(s):
Sialoprotein associated with cones and rods proteoglycan
Short name:
Spacrcan
Gene namesi
Name:Impg2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:3044955 Impg2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 1106ExtracellularSequence analysisAdd BLAST1079
Transmembranei1107 – 1127HelicalSequence analysisAdd BLAST21
Topological domaini1128 – 1243CytoplasmicSequence analysisAdd BLAST116

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi255R → H: Decreased-binding affinity to hyaluronan. 1 Publication1
Mutagenesisi263R → H: Decreased-binding affinity to hyaluronan. 1 Publication1
Mutagenesisi1083R → Q: Decreased-binding affinity to hyaluronan. 1 Publication1
Mutagenesisi1091R → Q: Decreased-binding affinity to hyaluronan. 1 Publication1
Mutagenesisi1136 – 1139RRER → SSES: Most important decrease in binding affinity to hyaluronan. 1 Publication4
Mutagenesisi1147R → G: Important decrease in binding affinity to hyaluronan. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000032015028 – 1243Interphotoreceptor matrix proteoglycan 2Add BLAST1216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi225N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi297N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi316N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi366N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi427O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi428O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi429O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi582N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi701O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi704O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi712O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi817O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi888O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi945N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi959N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1017 ↔ 1028PROSITE-ProRule annotation
Disulfide bondi1022 ↔ 1039PROSITE-ProRule annotation
Disulfide bondi1041 ↔ 1053PROSITE-ProRule annotation
Disulfide bondi1057 ↔ 1070PROSITE-ProRule annotation
Disulfide bondi1064 ↔ 1080PROSITE-ProRule annotation
Disulfide bondi1082 ↔ 1095PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ80XH2
PRIDEiQ80XH2

PTM databases

iPTMnetiQ80XH2
PhosphoSitePlusiQ80XH2

Expressioni

Tissue specificityi

Expressed in the retina and pineal gland.1 Publication

Developmental stagei

Increasing expression in retina from E15 to adulthood: expressed at P8 when photoreceptor outer segments are in active stages of elongation; elevated expression at P10 in the developing IPM and at P15 in the region adjacent to the retina pigment epithelium (RPE). From P18 to P35, more homogeneously present in the IPM surrounding both cones and rods.1 Publication

Gene expression databases

BgeeiENSMUSG00000035270 Expressed in 23 organ(s), highest expression level in embryo
CleanExiMM_IMPG2

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000063648

Structurei

3D structure databases

ProteinModelPortaliQ80XH2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini235 – 349SEA 1PROSITE-ProRule annotationAdd BLAST115
Domaini900 – 1013SEA 2PROSITE-ProRule annotationAdd BLAST114
Domaini1013 – 1054EGF-like 1PROSITE-ProRule annotationAdd BLAST42
Domaini1055 – 1096EGF-like 2PROSITE-ProRule annotationAdd BLAST42

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni255 – 263Hyaluronan-binding motif involved in chondroitin sulfate A-binding9
Regioni1083 – 1091Hyaluronan-binding motif involved in chondroitin sulfate C-binding9
Regioni1128 – 1136Hyaluronan-binding motif involved in chondroitin sulfate A- and C-binding9
Regioni1139 – 1147Hyaluronan-binding motif involved in chondroitin sulfate C-binding9
Regioni1212 – 1220Hyaluronan-binding motif involved in chondroitin sulfate A- and C-binding motif9

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IH0G Eukaryota
ENOG410Y9FF LUCA
GeneTreeiENSGT00530000063503
HOGENOMiHOG000113064
HOVERGENiHBG108006
InParanoidiQ80XH2
KOiK19017
OMAiWDLISLH
OrthoDBiEOG091G00XU
PhylomeDBiQ80XH2
TreeFamiTF331340

Family and domain databases

Gene3Di3.30.70.960, 1 hit
InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR032975 IMPG2
IPR000082 SEA_dom
IPR036364 SEA_dom_sf
PANTHERiPTHR12199:SF4 PTHR12199:SF4, 1 hit
PfamiView protein in Pfam
PF01390 SEA, 2 hits
SMARTiView protein in SMART
SM00200 SEA, 2 hits
SUPFAMiSSF82671 SSF82671, 2 hits
PROSITEiView protein in PROSITE
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 2 hits
PS50024 SEA, 2 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q80XH2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MIMFLPVGRM SLGILILFLT GGNLVSASEE RQEPMHAVSV LSPEKSTDLS
60 70 80 90 100
LPTRKRQLLD ATETGRRWLL RRRRSILFPN GVKICSSETV AEAVANHVKY
110 120 130 140 150
FKARVCQEAI WEAFRTFWDR LPGRDEYRHW MNLCEDGVTS VFEMGAHFSQ
160 170 180 190 200
SVEHRNLIMK KLAYTREAES SSCKDQSCGP ELSFPVPIGE TSTLTGAVSS
210 220 230 240 250
ASYPGLASES SAASPQESIS NEIENVTEEP TQPAAEQIAE FSIQLLGKRY
260 270 280 290 300
SEELRDPSSA LYRLLVEEFI SEVEKAFTGL PGYKGIRVLE FRAPEENDSG
310 320 330 340 350
IDVHYAVTFN GEAISNTTWD LISLHSNKVE NHGLVEMDDK PTAVYTISNF
360 370 380 390 400
RDYIAETLHQ NFLMGNSSLN PDPKSLQLIN VRGVLLPQTE DIVWNTQSSS
410 420 430 440 450
LQVTTSSILD NTLQAEWLSA DTTTTTTTTI SPFGFSSSSP SATGRELQSQ
460 470 480 490 500
SALRDVVSTS KLASPTKVVL SSLPEILGGS SLTLHSVTPA VLQPDLPVAP
510 520 530 540 550
EGRTSGSFIL EDGLASTEEL EDTSIDGLPS SPLIQPVPKE TVPPMEDSDT
560 570 580 590 600
ALLSTPHLTS SAIEDLTKDI GTPSGLESLA SNISDQLEVI PWFPDTSVEK
610 620 630 640 650
DFIFESGLGS GSGKDVDVID WPWSETSLEK TTKPLSKSWS EEQDALLPTE
660 670 680 690 700
GREKLHIDGR VDSTEQIIES SEHRYGDRPI HFIEEESHVR STIPIFVESA
710 720 730 740 750
TPPTSPIFSK HTSDVPDIDS YSLTKPPFLP VTIAIPASTK KTDEVLKEDM
760 770 780 790 800
VHTESSSHKE LDSEVPVSRP DMQPVWTMLP ESDTVWTRTS SLGKLSRDTL
810 820 830 840 850
ASTPESTDRL WLKASMTQST ELPSTTHSTQ LEEEVIMAVQ DISLELDQVG
860 870 880 890 900
TDYYQSELTE EQHGKADSYV EMSTSVHYTE MPIVALPTKG GVLSHTQTAG
910 920 930 940 950
ALVVFFSLRV TNMLFSEDLF NKNSLEYKAL EQRFLELLVP YLQSNLSGFQ
960 970 980 990 1000
NLEILSFRNG SIVVNSRVRF AESAPPNVNK AMYRILEDFC TTAYQTMNLD
1010 1020 1030 1040 1050
IDKYSLDVES GDEANPCKFQ ACNEFSECLV NPWSGEAKCK CYPGYLSVDE
1060 1070 1080 1090 1100
LPCQSLCDLQ PDFCLNDGKC DIMPGHGAIC RCRVGSNWWY RGQHCEEFVS
1110 1120 1130 1140 1150
EPFVIGITIA SVVSFLLVAS AVVFFLVKML QAQNVRRERQ RPTSSSRHPD
1160 1170 1180 1190 1200
SLSSVENAMK YNPAYESHLA GCELYEKSYS QHPFYSSASE EVIGGLSREE
1210 1220 1230 1240
IRQMYESSDL SKEEIQERMR ILELYANDPE FAAFVREHQM EEL
Length:1,243
Mass (Da):138,102
Last modified:June 1, 2003 - v1
Checksum:i26C647136491E4D5
GO
Isoform 2 (identifier: Q80XH2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     381-489: Missing.

Show »
Length:1,134
Mass (Da):126,711
Checksum:iAC73BF2D09D69CFB
GO

Sequence cautioni

The sequence AAO21221 differs from that shown. Reason: Frameshift at position 1236.Curated
The sequence AAO21221 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_031612381 – 489Missing in isoform 2. 1 PublicationAdd BLAST109

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY174061 mRNA Translation: AAO21221.1 Sequence problems.
BC048863 mRNA Translation: AAH48863.1
AK047356 mRNA Translation: BAC33032.1
CCDSiCCDS37358.1 [Q80XH2-1]
RefSeqiNP_777365.2, NM_174876.3 [Q80XH2-1]
UniGeneiMm.151998

Genome annotation databases

EnsembliENSMUST00000069936; ENSMUSP00000063648; ENSMUSG00000035270 [Q80XH2-1]
ENSMUST00000160116; ENSMUSP00000125135; ENSMUSG00000035270 [Q80XH2-2]
GeneIDi224224
KEGGimmu:224224
UCSCiuc007zmm.1 mouse [Q80XH2-1]
uc012agq.1 mouse [Q80XH2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiIMPG2_MOUSE
AccessioniPrimary (citable) accession number: Q80XH2
Secondary accession number(s): Q810Y3, Q8C8E4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 1, 2003
Last modified: September 12, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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