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Protein

Ras-related GTP-binding protein A

Gene

Rraga

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death.By similarity

Activity regulationi

The activation of GTP-binding proteins is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP). The GATOR1 complex functions as a GAP and stimulates RRAGA GTPase activity to turn it into its inactive GDP-bound form.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 21GTPBy similarity8
Nucleotide bindingi62 – 66GTPBy similarity5
Nucleotide bindingi127 – 130GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processApoptosis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1632852 Macroautophagy
R-MMU-165159 mTOR signalling
R-MMU-166208 mTORC1-mediated signalling
R-MMU-380972 Energy dependent regulation of mTOR by LKB1-AMPK
R-MMU-5628897 TP53 Regulates Metabolic Genes
R-MMU-8943724 Regulation of PTEN gene transcription

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related GTP-binding protein ACurated
Short name:
Rag ABy similarity
Short name:
RagABy similarity
Gene namesi
Name:RragaImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1915691 Rraga

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Lysosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002399461 – 313Ras-related GTP-binding protein AAdd BLAST313

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki142Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki220Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki230Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki244Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei309PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated. 'Lys-68'-linked polyubiquitination of the GDP-bound inactive form of RRAGA at Lys-142, Lys-220, Lys-230 and Lys-244 by RNF152 is increased in response to amino acid starvation. Polyubiquitination promotes interaction with the GATOR1 complex. This does not affect RRAGA degradation.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ80X95
MaxQBiQ80X95
PaxDbiQ80X95
PeptideAtlasiQ80X95
PRIDEiQ80X95

PTM databases

iPTMnetiQ80X95
PhosphoSitePlusiQ80X95
SwissPalmiQ80X95

Expressioni

Gene expression databases

BgeeiENSMUSG00000070934 Expressed in 288 organ(s), highest expression level in secondary oocyte
CleanExiMM_RRAGA
GenevisibleiQ80X95 MM

Interactioni

Subunit structurei

Can occur as a homodimer or as a heterodimer with RRAGC or RRAGD in a sequence-independent manner; heterodimerization stabilizes proteins of the heterodimer. In complex with RRAGC, but not with RRAGB, interacts with RPTOR. The GTP-bound form of RRAGA interacts with NOL8. Interacts with SH3BP4; the interaction with this negative regulator is most probably direct, preferentially occurs with the inactive GDP-bound form of RRAGA and is negatively regulated by amino acids. The Rag heterodimer interacts with SLC38A9; the probable amino acid sensor. Interacts (inactive GDP-bound form) with RNF152; stimulated by amino acid starvation. Interacts (polyubiquitinated) with the GATOR1 complex; inactivates RRAGA. Interacts (polyubiquitinated) with TSC2 (By similarity). Interacts with SESN1, SESN2 AND SESN3 (PubMed:25259925).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ80X95, 4 interactors
STRINGi10090.ENSMUSP00000088591

Structurei

3D structure databases

ProteinModelPortaliQ80X95
SMRiQ80X95
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GTR/RAG GTP-binding protein family.Curated

Phylogenomic databases

eggNOGiKOG3886 Eukaryota
ENOG410XQ0R LUCA
GeneTreeiENSGT00550000074769
HOGENOMiHOG000173258
HOVERGENiHBG052715
InParanoidiQ80X95
KOiK16185
OMAiIRINIRM
OrthoDBiEOG091G050Q
PhylomeDBiQ80X95
TreeFamiTF300616

Family and domain databases

CDDicd11384 RagA_like, 1 hit
InterProiView protein in InterPro
IPR006762 Gtr1_RagA
IPR027417 P-loop_NTPase
IPR039397 RagA/B
PANTHERiPTHR11259 PTHR11259, 2 hits
PfamiView protein in Pfam
PF04670 Gtr1_RagA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequencei

Sequence statusi: Complete.

Q80X95-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPNTAMKKKV LLMGKSGSGK TSMRSIIFAN YIARDTRRLG ATIDVEHSHV
60 70 80 90 100
RFLGNLVLNL WDCGGQDTFM ENYFTSQRDN IFRNVEVLIY VFDVESRELE
110 120 130 140 150
KDMHYYQSCL EAILQNSPDA KIFCLVHKMD LVQEDQRDLI FKEREEDLRR
160 170 180 190 200
LSRPLECACF RTSIWDETLY KAWSSIVYQL IPNVQQLEMN LRNFAQIIEA
210 220 230 240 250
DEVLLFERAT FLVISHYQCK EQRDVHRFEK ISNIIKQFKL SCSKLAASFQ
260 270 280 290 300
SMEVRNSNFA AFIDIFTSNT YVMVVMSDPS IPSAATLINI RNARKHFEKL
310
ERVDGPKHSL LMR
Length:313
Mass (Da):36,566
Last modified:June 1, 2003 - v1
Checksum:iB0DA1FC8FA6B766A
GO

Sequence cautioni

The sequence AAH37615 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC25103 differs from that shown. Reason: Frameshift at positions 183 and 193.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004955 mRNA Translation: BAC25103.1 Frameshift.
AK144591 mRNA Translation: BAE25953.1
AL824707 Genomic DNA No translation available.
BC037615 mRNA Translation: AAH37615.1 Different initiation.
BC048245 mRNA Translation: AAH48245.1
CCDSiCCDS18306.1
RefSeqiNP_848463.1, NM_178376.3
UniGeneiMm.31178

Genome annotation databases

EnsembliENSMUST00000091064; ENSMUSP00000088591; ENSMUSG00000070934
GeneIDi68441
KEGGimmu:68441
UCSCiuc008tlw.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004955 mRNA Translation: BAC25103.1 Frameshift.
AK144591 mRNA Translation: BAE25953.1
AL824707 Genomic DNA No translation available.
BC037615 mRNA Translation: AAH37615.1 Different initiation.
BC048245 mRNA Translation: AAH48245.1
CCDSiCCDS18306.1
RefSeqiNP_848463.1, NM_178376.3
UniGeneiMm.31178

3D structure databases

ProteinModelPortaliQ80X95
SMRiQ80X95
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80X95, 4 interactors
STRINGi10090.ENSMUSP00000088591

PTM databases

iPTMnetiQ80X95
PhosphoSitePlusiQ80X95
SwissPalmiQ80X95

Proteomic databases

EPDiQ80X95
MaxQBiQ80X95
PaxDbiQ80X95
PeptideAtlasiQ80X95
PRIDEiQ80X95

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000091064; ENSMUSP00000088591; ENSMUSG00000070934
GeneIDi68441
KEGGimmu:68441
UCSCiuc008tlw.2 mouse

Organism-specific databases

CTDi10670
MGIiMGI:1915691 Rraga

Phylogenomic databases

eggNOGiKOG3886 Eukaryota
ENOG410XQ0R LUCA
GeneTreeiENSGT00550000074769
HOGENOMiHOG000173258
HOVERGENiHBG052715
InParanoidiQ80X95
KOiK16185
OMAiIRINIRM
OrthoDBiEOG091G050Q
PhylomeDBiQ80X95
TreeFamiTF300616

Enzyme and pathway databases

ReactomeiR-MMU-1632852 Macroautophagy
R-MMU-165159 mTOR signalling
R-MMU-166208 mTORC1-mediated signalling
R-MMU-380972 Energy dependent regulation of mTOR by LKB1-AMPK
R-MMU-5628897 TP53 Regulates Metabolic Genes
R-MMU-8943724 Regulation of PTEN gene transcription

Miscellaneous databases

ChiTaRSiRraga mouse
PROiPR:Q80X95
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000070934 Expressed in 288 organ(s), highest expression level in secondary oocyte
CleanExiMM_RRAGA
GenevisibleiQ80X95 MM

Family and domain databases

CDDicd11384 RagA_like, 1 hit
InterProiView protein in InterPro
IPR006762 Gtr1_RagA
IPR027417 P-loop_NTPase
IPR039397 RagA/B
PANTHERiPTHR11259 PTHR11259, 2 hits
PfamiView protein in Pfam
PF04670 Gtr1_RagA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRRAGA_MOUSE
AccessioniPrimary (citable) accession number: Q80X95
Secondary accession number(s): B1AXR0, Q8C1S2, Q8CFU3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 1, 2003
Last modified: November 7, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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