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Protein

E3 ubiquitin-protein ligase TRIM69

Gene

Trim69

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May have E3 ubiquitin-protein ligase activity. May play a role in apoptosis.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri42 – 83RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processApoptosis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRIM69 (EC:2.3.2.27)
Alternative name(s):
RING finger B-box coiled-coil transcription factor
RING finger protein 36
RING-type E3 ubiquitin transferase TRIM69Curated
Testis-specific RING finger protein
Tripartite motif-containing protein 69
Gene namesi
Name:Trim69
Synonyms:Rnf36, Trif
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1918178 Trim69

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002782371 – 500E3 ubiquitin-protein ligase TRIM69Add BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei342PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated. Phosphorylation is necessary for nuclear localization.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80X56
PRIDEiQ80X56

PTM databases

iPTMnetiQ80X56
PhosphoSitePlusiQ80X56

Expressioni

Tissue specificityi

Expressed in spermatid.1 Publication

Gene expression databases

BgeeiENSMUSG00000033368 Expressed in 25 organ(s), highest expression level in testis
CleanExiMM_TRIM69
GenevisibleiQ80X56 MM

Interactioni

Subunit structurei

Interacts with PML.1 Publication

Protein-protein interaction databases

BioGridi214351, 36 interactors
IntActiQ80X56, 40 interactors
STRINGi10090.ENSMUSP00000047627

Structurei

3D structure databases

ProteinModelPortaliQ80X56
SMRiQ80X56
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini306 – 500B30.2/SPRYPROSITE-ProRule annotationAdd BLAST195

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 153Necessary for nuclear localizationAdd BLAST153

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili217 – 256Sequence analysisAdd BLAST40

Domaini

The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity and for nuclear localization and aggregation.By similarity

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri42 – 83RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITFB Eukaryota
ENOG410XWXM LUCA
GeneTreeiENSGT00760000118838
HOGENOMiHOG000234133
HOVERGENiHBG001357
InParanoidiQ80X56
KOiK12034
OMAiQHISLEF
OrthoDBiEOG091G05W2
PhylomeDBiQ80X56
TreeFamiTF342569

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR006574 PRY
IPR003877 SPRY_dom
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF13765 PRY, 1 hit
PF00622 SPRY, 1 hit
PRINTSiPR01407 BUTYPHLNCDUF
SMARTiView protein in SMART
SM00589 PRY, 1 hit
SM00184 RING, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q80X56-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEVSSRPPSN FDPGNYVEMS DPTTTHLPSK VVIQDLTTEL HCPLCNDWFR
60 70 80 90 100
DPLMLTCGHN FCQDCIQSFW KVHSKETFCP DCKMLCQYSN CTFNLVLEKL
110 120 130 140 150
VEKIKKLPLL KGHPQCPEHG ENLKLFSKPE GKMICFQCKD ARLSMGQSKD
160 170 180 190 200
FLQISEAVRF FTEELAIYQS QLQTTLKELQ SLRTIQKDAI SAYKDNKIQL
210 220 230 240 250
QQNLSLEFLK LHQFLHNKEK DILNDLRDEG KLLNEEMEVN LNQIQEQCLV
260 270 280 290 300
AKDMLATIQA RMEQQNSFDF LTDITKLIES MEKGMKTIVP RQLIAKKLSL
310 320 330 340 350
GRFKGPIQYI IWKEMQAILS PGPSQLTLDP KTAHPNLVLS KSQTSVCHCD
360 370 380 390 400
VKQVMPDDPE RFDSSVAVLG SKGFTSGKWY WEIEVGKKTK WTIGVVRESI
410 420 430 440 450
IRKGSCPLTP EQGFWLLRLR NQTDLKALDL PSRSLTLGDL RRVGVYLDYE
460 470 480 490 500
GGQVSFYNAT TMTHLYTFSS VFQEKLFPYL CPCLNDGGEN KEPLHIVHPQ
Length:500
Mass (Da):57,312
Last modified:June 1, 2003 - v1
Checksum:i607B0CEB02213754
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti117P → A in AAL41031 (PubMed:11578878).Curated1
Sequence conflicti355M → L in AAL41031 (PubMed:11578878).Curated1
Sequence conflicti362F → L in AAL41031 (PubMed:11578878).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334958 mRNA Translation: AAL41031.1
AL845457 Genomic DNA No translation available.
BC050815 mRNA Translation: AAH50815.1
CCDSiCCDS16655.1
RefSeqiNP_536771.2, NM_080510.2
UniGeneiMm.379188

Genome annotation databases

EnsembliENSMUST00000036089; ENSMUSP00000047627; ENSMUSG00000033368
GeneIDi70928
KEGGimmu:70928
UCSCiuc008mah.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334958 mRNA Translation: AAL41031.1
AL845457 Genomic DNA No translation available.
BC050815 mRNA Translation: AAH50815.1
CCDSiCCDS16655.1
RefSeqiNP_536771.2, NM_080510.2
UniGeneiMm.379188

3D structure databases

ProteinModelPortaliQ80X56
SMRiQ80X56
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214351, 36 interactors
IntActiQ80X56, 40 interactors
STRINGi10090.ENSMUSP00000047627

PTM databases

iPTMnetiQ80X56
PhosphoSitePlusiQ80X56

Proteomic databases

PaxDbiQ80X56
PRIDEiQ80X56

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036089; ENSMUSP00000047627; ENSMUSG00000033368
GeneIDi70928
KEGGimmu:70928
UCSCiuc008mah.1 mouse

Organism-specific databases

CTDi140691
MGIiMGI:1918178 Trim69

Phylogenomic databases

eggNOGiENOG410ITFB Eukaryota
ENOG410XWXM LUCA
GeneTreeiENSGT00760000118838
HOGENOMiHOG000234133
HOVERGENiHBG001357
InParanoidiQ80X56
KOiK12034
OMAiQHISLEF
OrthoDBiEOG091G05W2
PhylomeDBiQ80X56
TreeFamiTF342569

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

PROiPR:Q80X56
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033368 Expressed in 25 organ(s), highest expression level in testis
CleanExiMM_TRIM69
GenevisibleiQ80X56 MM

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR006574 PRY
IPR003877 SPRY_dom
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF13765 PRY, 1 hit
PF00622 SPRY, 1 hit
PRINTSiPR01407 BUTYPHLNCDUF
SMARTiView protein in SMART
SM00589 PRY, 1 hit
SM00184 RING, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRI69_MOUSE
AccessioniPrimary (citable) accession number: Q80X56
Secondary accession number(s): Q8VHZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: June 1, 2003
Last modified: November 7, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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