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Protein

Serine/threonine-protein kinase VRK1

Gene

Vrk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity (By similarity).By similarity2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Activity regulationi

Active in presence of Mn2+, Mg2+ and Zn2+, but is not functional with Ca2+ or Cu2+. Has a higher affinity for Mn2+ than for Mg2+. RAN inhibits its autophosphorylation and its ability to phosphorylate histone H3 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71ATPPROSITE-ProRule annotation1
Active sitei177Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi43 – 51ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2995383 Initiation of Nuclear Envelope Reformation

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase VRK1 (EC:2.7.11.1)
Alternative name(s):
Serine/threonine-protein kinase 51PK
Vaccinia-related kinase 1
Gene namesi
Name:Vrk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1261847 Vrk1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000868041 – 440Serine/threonine-protein kinase VRK1Add BLAST440

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki71Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei342Phosphoserine; by PLK3By similarity1
Modified residuei376PhosphoserineBy similarity1
Modified residuei378PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylation by PLK3 leads to induction of Golgi fragmentation during mitosis (By similarity). Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53.By similarity1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ80X41
PRIDEiQ80X41

PTM databases

iPTMnetiQ80X41
PhosphoSitePlusiQ80X41

Expressioni

Tissue specificityi

Highly expressed in testis. Expressed in liver, kidney and muscle. Weakly expressed in thymus, bone marrow and spleen.2 Publications

Developmental stagei

Expressed from E10.5 to E14 in developing liver and then decreases. It increases again from E17.5 and remains thereafter. Highly expressed in hematopoietic embryonic tissues from E10.5 to E14.5. Weakly expressed in the yolk-sac.1 Publication

Gene expression databases

BgeeiENSMUSG00000021115 Expressed in 243 organ(s), highest expression level in bone marrow
CleanExiMM_VRK1
ExpressionAtlasiQ80X41 baseline and differential
GenevisibleiQ80X41 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204536, 2 interactors
IntActiQ80X41, 3 interactors
MINTiQ80X41
STRINGi10090.ENSMUSP00000021539

Structurei

3D structure databases

ProteinModelPortaliQ80X41
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 317Protein kinasePROSITE-ProRule annotationAdd BLAST281

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1164 Eukaryota
ENOG410XPGP LUCA
GeneTreeiENSGT00920000148988
HOGENOMiHOG000069991
HOVERGENiHBG007532
InParanoidiQ80X41
KOiK08816
OMAiYMRAAKP
OrthoDBiEOG091G05V5
PhylomeDBiQ80X41
TreeFamiTF106473

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q80X41-1) [UniParc]FASTAAdd to basket
Also known as: L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPRVKAAQAG RPGPAKRRLA EQFAAGEVLT DMSRKEWKLG LPIGQGGFGC
60 70 80 90 100
IYLADTNSSK PVGSDAPCVV KVEPSDNGPL FTELKFYQRA AKPEQIQKWI
110 120 130 140 150
RTHKLKYLGV PKYWGSGLHD KNGKSYRFMI MDRFGSDLQK IYEANAKRFS
160 170 180 190 200
RKTVLQLSLR ILDILEYIHE HEYVHGDIKA SNLLLSHKNP DQVYLVDYGL
210 220 230 240 250
AYRYCPDGVH KEYKEDPKRC HDGTLEFTSI DAHKGVAPSR RGDLEILGYC
260 270 280 290 300
MIQWLSGCLP WEDNLKDPNY VRDSKIRYRD NVAALMEKCF PEKNKPGEIA
310 320 330 340 350
KYMESVKLLE YTEKPLYQNL RDILLQGLKA IGSKDDGKLD FSAVENGSVK
360 370 380 390 400
TRPASKKRKK EAEESAVCAV EDMECSDTQV QEAAQTRSVE SQGAIHGSMS
410 420 430 440
QPAAGCSSSD SSRRQQHLGL EQDMLRLDRR GSRTRKKAQK
Length:440
Mass (Da):49,741
Last modified:October 10, 2003 - v2
Checksum:i8318BA76A44B16BF
GO
Isoform 2 (identifier: Q80X41-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     388-431: Missing.

Note: No experimental confirmation available.
Show »
Length:396
Mass (Da):45,032
Checksum:i9F0FD5E3E685E545
GO
Isoform 3 (identifier: Q80X41-3) [UniParc]FASTAAdd to basket
Also known as: S

The sequence of this isoform differs from the canonical sequence as follows:
     388-411: Missing.

Show »
Length:416
Mass (Da):47,478
Checksum:i6E15EE21E4180E26
GO
Isoform 5 (identifier: Q80X41-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     125-126: Missing.
     388-411: Missing.

Note: No experimental confirmation available.
Show »
Length:414
Mass (Da):47,228
Checksum:iA39A1A135715034B
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1Y7VMH3A0A1Y7VMH3_MOUSE
Serine/threonine-protein kinase VRK...
Vrk1
413Annotation score:
A0A1Y7VLL5A0A1Y7VLL5_MOUSE
Serine/threonine-protein kinase VRK...
Vrk1
63Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008528125 – 126Missing in isoform 5. 1 Publication2
Alternative sequenceiVSP_008529388 – 431Missing in isoform 2. 1 PublicationAdd BLAST44
Alternative sequenceiVSP_008532388 – 411Missing in isoform 3 and isoform 5. 2 PublicationsAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080252 mRNA Translation: AAC29496.1
AF080253 mRNA Translation: AAC29497.1
AK032344 mRNA Translation: BAC27825.1
AK088230 mRNA Translation: BAC40225.1
AK088674 mRNA Translation: BAC40497.1
BC016676 mRNA Translation: AAH16676.1
CCDSiCCDS26157.1 [Q80X41-3]
CCDS26158.1 [Q80X41-2]
CCDS36551.1 [Q80X41-1]
RefSeqiNP_001025014.1, NM_001029843.1 [Q80X41-3]
NP_001025015.1, NM_001029844.1 [Q80X41-2]
NP_035835.1, NM_011705.3 [Q80X41-1]
XP_006515872.1, XM_006515809.2 [Q80X41-1]
XP_006515873.1, XM_006515810.3 [Q80X41-1]
XP_006515874.1, XM_006515811.3 [Q80X41-1]
XP_006515875.1, XM_006515812.2 [Q80X41-1]
XP_017170535.1, XM_017315046.1 [Q80X41-2]
UniGeneiMm.2981

Genome annotation databases

EnsembliENSMUST00000021539; ENSMUSP00000021539; ENSMUSG00000021115 [Q80X41-1]
ENSMUST00000072040; ENSMUSP00000071922; ENSMUSG00000021115 [Q80X41-2]
ENSMUST00000085026; ENSMUSP00000082101; ENSMUSG00000021115 [Q80X41-3]
ENSMUST00000220629; ENSMUSP00000152109; ENSMUSG00000021115 [Q80X41-1]
GeneIDi22367
KEGGimmu:22367
UCSCiuc007ozb.1 mouse [Q80X41-1]
uc007ozd.1 mouse [Q80X41-2]
uc007oze.1 mouse [Q80X41-5]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiVRK1_MOUSE
AccessioniPrimary (citable) accession number: Q80X41
Secondary accession number(s): O88635
, O88636, Q8C2P7, Q91YH9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 10, 2003
Last modified: September 12, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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