UniProtKB - Q80W66 (HS3S2_RAT)
Protein
Heparan sulfate glucosamine 3-O-sulfotransferase 2
Gene
Hs3st2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Catalyzes the O-sulfation of glucosamine in GlcA2S-GlcNS. May contribute to the pineal circadian physiology. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate.1 Publication
Catalytic activityi
- 3'-phosphoadenylyl sulfate + α-D-glucosaminyl-[heparan sulfate](n) = 3-sulfo-α-D-glucosaminyl-[heparan sulfate](n) + adenosine 3',5'-bisphosphate + H+EC:2.8.2.29
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 205 | PAPSBy similarity | 1 | |
Binding sitei | 213 | PAPSBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 124 – 128 | PAPSBy similarity | 5 | |
Nucleotide bindingi | 330 – 334 | PAPSBy similarity | 5 |
GO - Molecular functioni
- [heparan sulfate]-glucosamine 3-sulfotransferase 1 activity Source: GO_Central
- [heparan sulfate]-glucosamine 3-sulfotransferase 2 activity Source: UniProtKB-EC
GO - Biological processi
- circadian rhythm Source: RGD
Keywordsi
Molecular function | Transferase |
Enzyme and pathway databases
Reactomei | R-RNO-2022928, HS-GAG biosynthesis |
Names & Taxonomyi
Protein namesi | Recommended name: Heparan sulfate glucosamine 3-O-sulfotransferase 2 (EC:2.8.2.29)Alternative name(s): Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 2 Short name: Heparan sulfate 3-O-sulfotransferase 2 |
Gene namesi | Name:Hs3st2 Synonyms:3ost2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 727787, Hs3st2 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane Curated; Single-pass type II membrane protein Curated
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 19 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 20 – 39 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 20 | |
Topological domaini | 40 – 367 | LumenalSequence analysisAdd BLAST | 328 |
Keywords - Cellular componenti
Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085216 | 1 – 367 | Heparan sulfate glucosamine 3-O-sulfotransferase 2Add BLAST | 367 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 102 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 193 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 235 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 306 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 313 ↔ 325 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q80W66 |
PTM databases
GlyGeni | Q80W66, 4 sites |
iPTMneti | Q80W66 |
PhosphoSitePlusi | Q80W66 |
Expressioni
Tissue specificityi
Highly expressed in the brain, in the pineal gland specifically during the daylight hours, and weakly in the olfactory bulb and pineal gland during the night. First detected between postnatal days 5 and 10.1 Publication
Inductioni
Activated by light stimulation at night through the suppression of beta-adrenergic signaling.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000017659, Expressed in brain and 14 other tissues |
Genevisiblei | Q80W66, RN |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 146 – 152 | Substrate bindingBy similarity | 7 | |
Regioni | 177 – 180 | Substrate bindingBy similarity | 4 | |
Regioni | 245 – 246 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the sulfotransferase 1 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3704, Eukaryota |
GeneTreei | ENSGT00940000160498 |
HOGENOMi | CLU_017703_0_1_1 |
InParanoidi | Q80W66 |
OMAi | CYSLLCC |
OrthoDBi | 712400at2759 |
PhylomeDBi | Q80W66 |
TreeFami | TF350755 |
Family and domain databases
InterProi | View protein in InterPro IPR037359, NST/OST IPR027417, P-loop_NTPase IPR000863, Sulfotransferase_dom |
PANTHERi | PTHR10605, PTHR10605, 1 hit |
Pfami | View protein in Pfam PF00685, Sulfotransfer_1, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
i Sequence
Sequence statusi: Complete.
Q80W66-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAYRVLGRAG PPQPRRARRL LFAFTLSLSC TYLCYSFLCC CDGLGQSRLL
60 70 80 90 100
GAPRCLRGPS ASGQKLLAKS RPCDPPGPTP SEPSAPSAPA AAAPAPRLSG
110 120 130 140 150
SNHSGSPKPG TKRLPQALIV GVKKGGTRAV LEFIRVHPDV RALGTEPHFF
160 170 180 190 200
DRNYGRGLDW YRSLMPRTLE TQITLEKTPS YFVTQEAPRR IFNMSRDTKL
210 220 230 240 250
IVVVRNPVTR AISDYTQTLS KKPDIPTFEG LSFRNRSLGL VDVSWNAIRI
260 270 280 290 300
GMYALHLESW LRYFPLAQIH FVSGERLITD PAGEMGRIQD FLGIKRFITD
310 320 330 340 350
KHFYFNKTKG FPCLKKPEST LLPRCLGKSK GRTHVQIDPE VIDQLREFYR
360
PYNIKFYETV GQDFRWE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY240873 mRNA Translation: AAP30887.1 BC128721 mRNA Translation: AAI28722.1 |
RefSeqi | NP_852035.1, NM_181370.2 |
Genome annotation databases
Ensembli | ENSRNOT00000023773; ENSRNOP00000023773; ENSRNOG00000017659 |
GeneIDi | 293451 |
KEGGi | rno:293451 |
UCSCi | RGD:727787, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY240873 mRNA Translation: AAP30887.1 BC128721 mRNA Translation: AAI28722.1 |
RefSeqi | NP_852035.1, NM_181370.2 |
3D structure databases
SMRi | Q80W66 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000023773 |
PTM databases
GlyGeni | Q80W66, 4 sites |
iPTMneti | Q80W66 |
PhosphoSitePlusi | Q80W66 |
Proteomic databases
PaxDbi | Q80W66 |
Genome annotation databases
Ensembli | ENSRNOT00000023773; ENSRNOP00000023773; ENSRNOG00000017659 |
GeneIDi | 293451 |
KEGGi | rno:293451 |
UCSCi | RGD:727787, rat |
Organism-specific databases
CTDi | 9956 |
RGDi | 727787, Hs3st2 |
Phylogenomic databases
eggNOGi | KOG3704, Eukaryota |
GeneTreei | ENSGT00940000160498 |
HOGENOMi | CLU_017703_0_1_1 |
InParanoidi | Q80W66 |
OMAi | CYSLLCC |
OrthoDBi | 712400at2759 |
PhylomeDBi | Q80W66 |
TreeFami | TF350755 |
Enzyme and pathway databases
Reactomei | R-RNO-2022928, HS-GAG biosynthesis |
Miscellaneous databases
PROi | PR:Q80W66 |
Gene expression databases
Bgeei | ENSRNOG00000017659, Expressed in brain and 14 other tissues |
Genevisiblei | Q80W66, RN |
Family and domain databases
InterProi | View protein in InterPro IPR037359, NST/OST IPR027417, P-loop_NTPase IPR000863, Sulfotransferase_dom |
PANTHERi | PTHR10605, PTHR10605, 1 hit |
Pfami | View protein in Pfam PF00685, Sulfotransfer_1, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HS3S2_RAT | |
Accessioni | Q80W66Primary (citable) accession number: Q80W66 Secondary accession number(s): A1A5M1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2005 |
Last sequence update: | June 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 114 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families