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Protein

WD repeat domain phosphoinositide-interacting protein 2

Gene

Wipi2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Omegasomes are derived from ER and serve as intermediates for genesis of isolation membrane (also called preautophagosome) from ER cisternae membranes.3 Publications

GO - Molecular functioni

GO - Biological processi

  • autophagosome assembly Source: UniProtKB
  • autophagy of mitochondrion Source: GO_Central
  • protein lipidation Source: GO_Central
  • protein lipidation involved in autophagosome assembly Source: MGI
  • protein localization to phagophore assembly site Source: MGI
  • xenophagy Source: MGI

Keywordsi

Biological processAutophagy

Enzyme and pathway databases

ReactomeiR-MMU-1632852 Macroautophagy

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat domain phosphoinositide-interacting protein 2
Short name:
WIPI-2
Gene namesi
Name:Wipi2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1923831 Wipi2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000514411 – 445WD repeat domain phosphoinositide-interacting protein 2Add BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei395PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ80W47
MaxQBiQ80W47
PaxDbiQ80W47
PeptideAtlasiQ80W47
PRIDEiQ80W47

PTM databases

iPTMnetiQ80W47
PhosphoSitePlusiQ80W47

Expressioni

Gene expression databases

BgeeiENSMUSG00000029578 Expressed in 291 organ(s), highest expression level in embryo
CleanExiMM_WIPI2
ExpressionAtlasiQ80W47 baseline and differential
GenevisibleiQ80W47 MM

Interactioni

Subunit structurei

Interacts with TECPR1 (By similarity). Intercats with ATG16L1.By similarity1 Publication

Protein-protein interaction databases

BioGridi217015, 11 interactors
IntActiQ80W47, 10 interactors
STRINGi10090.ENSMUSP00000045201

Structurei

3D structure databases

ProteinModelPortaliQ80W47
SMRiQ80W47
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati182 – 222WD 1Sequence analysisAdd BLAST41
Repeati228 – 267WD 2Sequence analysisAdd BLAST40
Repeati311 – 349WD 3Sequence analysisAdd BLAST39

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi223 – 226FRRG motif1 Publication4

Domaini

The FRRG motif is required for recruitment to PtdIns3P.1 Publication

Sequence similaritiesi

Belongs to the WD repeat SVP1 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2110 Eukaryota
ENOG410XP6Y LUCA
GeneTreeiENSGT00730000110845
HOGENOMiHOG000217544
HOVERGENiHBG056639
InParanoidiQ80W47
KOiK17908
OrthoDBiEOG091G08ZS
PhylomeDBiQ80W47
TreeFamiTF314879

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR036322 WD40_repeat_dom_sf
IPR032911 WIPI2
PANTHERiPTHR11227:SF27 PTHR11227:SF27, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 3 hits
SUPFAMiSSF50978 SSF50978, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q80W47-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNLASQSGEA GAGQLLFANF NQDNTSLAVG SKSGYKFFSL SSVDKLEQIY
60 70 80 90 100
ECTDTEDVCI VERLFSSSLV AIVSLKAPRK LKVCHFKKGT EICNYSYSNT
110 120 130 140 150
ILAVKLNRQR LIVCLEESLY IHNIRDMKVL HTIRETPPNP AGLCALSINN
160 170 180 190 200
DNCYLAYPGS ASIGEVQVFD TINLRAANMI PAHDSPLAAL AFDASGTKLA
210 220 230 240 250
TASEKGTVIR VFSIPEGQKL FEFRRGVKRC VSICSLAFSM DGMFLSASSN
260 270 280 290 300
TETVHIFKLE AVREKPPEEP TTWTGYFGKV LMASTSYLPS QVTEMFNQGR
310 320 330 340 350
AFATVRLPFC GHKNICSLTT IQKIPRLLVG ASDGYLYMYN LDPQEGGECA
360 370 380 390 400
LMRQHRLDGS METTSEIVDS ASHDCPLATQ TYGTAAAKGA YVPSSPTRLG
410 420 430 440
KGQDANLEAY TDDLGAVGGA CLEDEASALR LDEDSEHPPM ILRTD
Length:445
Mass (Da):48,477
Last modified:June 1, 2003 - v1
Checksum:iA4D4FF973B104D93
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YWK1D3YWK1_MOUSE
WD repeat domain phosphoinositide-i...
Wipi2
425Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK155113 mRNA Translation: BAE33057.1
AK169051 mRNA Translation: BAE40839.1
AK172030 mRNA Translation: BAE42786.1
BC044894 mRNA Translation: AAH44894.1
CCDSiCCDS19828.1
RefSeqiNP_848485.1, NM_178398.4
UniGeneiMm.29432

Genome annotation databases

EnsembliENSMUST00000036872; ENSMUSP00000045201; ENSMUSG00000029578
GeneIDi74781
KEGGimmu:74781
UCSCiuc009aiy.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK155113 mRNA Translation: BAE33057.1
AK169051 mRNA Translation: BAE40839.1
AK172030 mRNA Translation: BAE42786.1
BC044894 mRNA Translation: AAH44894.1
CCDSiCCDS19828.1
RefSeqiNP_848485.1, NM_178398.4
UniGeneiMm.29432

3D structure databases

ProteinModelPortaliQ80W47
SMRiQ80W47
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217015, 11 interactors
IntActiQ80W47, 10 interactors
STRINGi10090.ENSMUSP00000045201

PTM databases

iPTMnetiQ80W47
PhosphoSitePlusiQ80W47

Proteomic databases

EPDiQ80W47
MaxQBiQ80W47
PaxDbiQ80W47
PeptideAtlasiQ80W47
PRIDEiQ80W47

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036872; ENSMUSP00000045201; ENSMUSG00000029578
GeneIDi74781
KEGGimmu:74781
UCSCiuc009aiy.1 mouse

Organism-specific databases

CTDi26100
MGIiMGI:1923831 Wipi2

Phylogenomic databases

eggNOGiKOG2110 Eukaryota
ENOG410XP6Y LUCA
GeneTreeiENSGT00730000110845
HOGENOMiHOG000217544
HOVERGENiHBG056639
InParanoidiQ80W47
KOiK17908
OrthoDBiEOG091G08ZS
PhylomeDBiQ80W47
TreeFamiTF314879

Enzyme and pathway databases

ReactomeiR-MMU-1632852 Macroautophagy

Miscellaneous databases

ChiTaRSiWipi2 mouse
PROiPR:Q80W47
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029578 Expressed in 291 organ(s), highest expression level in embryo
CleanExiMM_WIPI2
ExpressionAtlasiQ80W47 baseline and differential
GenevisibleiQ80W47 MM

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR036322 WD40_repeat_dom_sf
IPR032911 WIPI2
PANTHERiPTHR11227:SF27 PTHR11227:SF27, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 3 hits
SUPFAMiSSF50978 SSF50978, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWIPI2_MOUSE
AccessioniPrimary (citable) accession number: Q80W47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2003
Last modified: November 7, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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