UniProtKB - Q80VW7 (AKNA_MOUSE)
Microtubule organization protein AKNA
Akna
Functioni
Centrosomal protein that plays a key role in cell delamination by regulating microtubule organization (PubMed:30787442).
Required for the delamination and retention of neural stem cells from the subventricular zone during neurogenesis (PubMed:30787442).
Also regulates the epithelial-to-mesenchymal transition in other epithelial cells (PubMed:30787442).
Acts by increasing centrosomal microtubule nucleation and recruiting nucleation factors and minus-end stabilizers, thereby destabilizing microtubules at the adherens junctions and mediating constriction of the apical endfoot (PubMed:30787442).
In addition, may also act as a transcription factor that specifically activates the expression of the CD40 receptor and its ligand CD40L/CD154, two cell surface molecules on lymphocytes that are critical for antigen-dependent-B-cell development (By similarity).
Binds to A/T-rich promoters (By similarity).
It is unclear how it can both act as a microtubule organizer and as a transcription factor; additional evidences are required to reconcile these two apparently contradictory functions (Probable).
By similarityCurated1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 1088 – 1096 | A.T hook | 9 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
GO - Biological processi
- delamination Source: UniProtKB
- epithelial to mesenchymal transition Source: UniProtKB
- neuroblast delamination Source: UniProtKB
- neuroblast division in subventricular zone Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of inflammatory response Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Neurogenesis, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Microtubule organization protein AKNACuratedAlternative name(s): AT-hook-containing transcription factorCurated |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2140340, Akna |
VEuPathDBi | HostDB:ENSMUSG00000039158 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytoskeleton
- centriole 1 Publication
Note: Localizes to the distal part of the subdistal appendages of the mother centriole in interphase (PubMed:30787442). Also found at the proximal ends of centrioles and along microtubules (PubMed:30787442). The centrosomal localization is dependent on centrioles (PubMed:30787442). Dissociates from centrosomes during M-phase without proteolytic degradation and reassembles at the centrosomes during late telophase and early G1 phase (PubMed:30787442). Dissociation and reassembly is regulated by phosphorylation (PubMed:30787442).1 Publication
Cytoskeleton
- centriole Source: UniProtKB
- centrosome Source: UniProtKB
- microtubule Source: UniProtKB
Cytosol
- cytosol Source: MGI
Nucleus
- fibrillar center Source: MGI
- nucleoplasm Source: MGI
Other locations
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Microtubule, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000289160 | 1 – 1404 | Microtubule organization protein AKNAAdd BLAST | 1404 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 51 | PhosphoserineCombined sources | 1 | |
Modified residuei | 302 | PhosphoserineBy similarity | 1 | |
Modified residuei | 485 | PhosphoserineBy similarity | 1 | |
Modified residuei | 520 | PhosphoserineBy similarity | 1 | |
Modified residuei | 617 | PhosphoserineCombined sources | 1 | |
Modified residuei | 750 | PhosphoserineBy similarity | 1 | |
Modified residuei | 753 | PhosphoserineBy similarity | 1 | |
Modified residuei | 831 | PhosphoserineBy similarity | 1 | |
Modified residuei | 860 | PhosphoserineBy similarity | 1 | |
Modified residuei | 971 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1144 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1145 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1200 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1339 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1352 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1389 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q80VW7 |
jPOSTi | Q80VW7 |
MaxQBi | Q80VW7 |
PaxDbi | Q80VW7 |
PeptideAtlasi | Q80VW7 |
PRIDEi | Q80VW7 |
ProteomicsDBi | 296154 [Q80VW7-1] 296155 [Q80VW7-2] 296156 [Q80VW7-3] 296157 [Q80VW7-4] |
PTM databases
iPTMneti | Q80VW7 |
PhosphoSitePlusi | Q80VW7 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000039158, Expressed in granulocyte and 256 other tissues |
Genevisiblei | Q80VW7, MM |
Interactioni
Subunit structurei
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000041614 |
Miscellaneous databases
RNActi | Q80VW7, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 304 | DisorderedSequence analysisAdd BLAST | 304 | |
Regioni | 317 – 382 | DisorderedSequence analysisAdd BLAST | 66 | |
Regioni | 494 – 549 | DisorderedSequence analysisAdd BLAST | 56 | |
Regioni | 645 – 708 | DisorderedSequence analysisAdd BLAST | 64 | |
Regioni | 754 – 787 | PESTAdd BLAST | 34 | |
Regioni | 755 – 1038 | DisorderedSequence analysisAdd BLAST | 284 | |
Regioni | 885 – 906 | PESTAdd BLAST | 22 | |
Regioni | 1085 – 1185 | DisorderedSequence analysisAdd BLAST | 101 | |
Regioni | 1208 – 1235 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 1253 – 1286 | DisorderedSequence analysisAdd BLAST | 34 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 22 – 36 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 53 – 71 | Basic and acidic residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 72 – 90 | Acidic residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 180 – 210 | Polar residuesSequence analysisAdd BLAST | 31 | |
Compositional biasi | 263 – 279 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 287 – 304 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 353 – 368 | Basic and acidic residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 516 – 549 | Polar residuesSequence analysisAdd BLAST | 34 | |
Compositional biasi | 658 – 708 | Polar residuesSequence analysisAdd BLAST | 51 | |
Compositional biasi | 760 – 776 | Acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 802 – 823 | Basic and acidic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 852 – 867 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 898 – 937 | Polar residuesSequence analysisAdd BLAST | 40 | |
Compositional biasi | 959 – 981 | Polar residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 1004 – 1038 | Polar residuesSequence analysisAdd BLAST | 35 | |
Compositional biasi | 1085 – 1105 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 1107 – 1134 | Basic and acidic residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 1215 – 1235 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 1254 – 1271 | Polar residuesSequence analysisAdd BLAST | 18 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QRSN, Eukaryota |
GeneTreei | ENSGT00940000154254 |
HOGENOMi | CLU_005641_0_0_1 |
InParanoidi | Q80VW7 |
OMAi | GTEVHWA |
OrthoDBi | 112521at2759 |
PhylomeDBi | Q80VW7 |
TreeFami | TF336885 |
Family and domain databases
InterProi | View protein in InterPro IPR022150, AKNA_dom |
Pfami | View protein in Pfam PF12443, AKNA, 1 hit |
s (4)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASSGAKAQW VGPSLGQGPR RRRWAWAEEQ DTDGRRDQGW GNSQSLPEAP
60 70 80 90 100
SPELLEDFRR AQEHLPPLEW DPDMQDSEES SGEETEADDA SSPEGSTVPL
110 120 130 140 150
PWLSRSNQQL DMSEEELDEA SGRPEVDLAG ESCTELECED QGDSSPPPPG
160 170 180 190 200
QGPAKGWVTF IKQGSNYRPS EHLEAQPSVE HSRTKSWSSG TVSLRQPSDS
210 220 230 240 250
LGSTWEGDTE VPQPSILPKA LPQSPCHNFP HPGDRNGGDV APATPTEFRD
260 270 280 290 300
SLAAPAQNAE CSAGTWGEET TSLPSSRPED QTWKRTKTSP KPLPSRFTGS
310 320 330 340 350
VSPLSTRLGA IKKVVPQHKQ GATLAGHSSS QAPKYGRGRR LNYPLPDFSK
360 370 380 390 400
VGPRVRFPKD ENYRPPKSRG HNRQQGSTRP LIFKSPAEIV RDVLLSSGEA
410 420 430 440 450
SLAKESSLAH TITRVPQEFQ TPEQATELVH QLQEDYHKLL TKYAEAENTI
460 470 480 490 500
DQLRLGAKVH LYSDPPQPSQ SFCSGSMPQG SKVLSFSIPQ PRVAEWWPDP
510 520 530 540 550
AQDPQASEAT GWPFPRTDLS PSSSPGVATP GRLPQSQGIA TDQPSTGQTQ
560 570 580 590 600
ALTSQASRLL AKVQSFEELV LAGHLPPQDQ IKSLEQLRAA HMALEAEYLQ
610 620 630 640 650
ACREELLDPQ LDASQGSPRT LNLCRELEAE IYHLGQRLEE LQDHMDQTQR
660 670 680 690 700
ETEPCRPDLQ DSTPTMSFLP QSAHLSMPSG PVSLPDGQTY QEPATTTTTS
710 720 730 740 750
PGSSCTLPIN KKLSLSIKTE ESPRGLPVPL RDRTLQVEQD FHGLLERYLS
760 770 780 790 800
VKSLPEALRD EDEDDLEEEE EEQDHQGPLE VDSPATAPGK TEAVRVPPGE
810 820 830 840 850
RPTQAEESHR DATQEDEEQM GPMKSPDFRP SMARDTYTPV LDTAEVAQRG
860 870 880 890 900
TKAMVSHQSS LTSLEESRPS ELLPRKALLR AGGPHTEEPW MVSPETDSGF
910 920 930 940 950
VGSETSIVSP FTQTPEHRLS HVSTSGPSAQ HLTASVPGDR TSHPKARGLM
960 970 980 990 1000
VPRRATETGI PRSRTQQHFS SLSSPGRGAQ SCHLEETSVA KIAVPRSEFK
1010 1020 1030 1040 1050
RQKQISKQLL PSGRTSPDSA PAPTAASTPH GSAESTANLL LNRTERDQAI
1060 1070 1080 1090 1100
KDLQAEVSRL RLQLEDSLHR PHPDGPACVA SAFNHSTQTQ EKLGSSPSWG
1110 1120 1130 1140 1150
PHYGSKSTER LSREPNGVEP AEPMGRRRAR SSSVPRDVPR LYLSSESESP
1160 1170 1180 1190 1200
APRLSSEKSR TFEEHPEAAQ WGTRPQSSSK RRERVSFRGQ YTGQEYHILS
1210 1220 1230 1240 1250
PKAILKDSGT PSCPHCHPIR TQDTGSAVSR DTTRGSSAAD TLRCALCGEV
1260 1270 1280 1290 1300
KSSAEADGSS SGPSEKNTPK KPSTPILKRK NRQTGSPVRM APGLWYLAAA
1310 1320 1330 1340 1350
PPAPAPPALA YISSAPIMPY LPPTVYYAAP APTSAQTASP QPARGPRRTR
1360 1370 1380 1390 1400
HSVQLGLNDL EELQAALREA AQAAENVRST TRQLSRSLSA DLRHARSLRG
SCLF
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 396 | S → R in BAE32751 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 527 | V → M in BAC98278 (PubMed:14621295).Curated | 1 | |
Sequence conflicti | 534 | P → S in BAC98278 (PubMed:14621295).Curated | 1 | |
Sequence conflicti | 558 | R → Q in BAC98278 (PubMed:14621295).Curated | 1 | |
Sequence conflicti | 712 | K → E in BAC98278 (PubMed:14621295).Curated | 1 | |
Sequence conflicti | 928 – 929 | Missing in BAC98278 (PubMed:14621295).Curated | 2 | |
Sequence conflicti | 928 – 929 | Missing in AAH25835 (PubMed:15489334).Curated | 2 | |
Sequence conflicti | 944 | P → S in BAC98278 (PubMed:14621295).Curated | 1 | |
Sequence conflicti | 944 | P → S in AAH25835 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 956 – 958 | TET → IEA in BAC98278 (PubMed:14621295).Curated | 3 | |
Sequence conflicti | 956 – 958 | TET → IEA in AAH25835 (PubMed:15489334).Curated | 3 | |
Sequence conflicti | 963 | S → T in BAC98278 (PubMed:14621295).Curated | 1 | |
Sequence conflicti | 963 | S → T in AAH25835 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 967 – 969 | QHF → RHL in BAC98278 (PubMed:14621295).Curated | 3 | |
Sequence conflicti | 967 – 969 | QHF → RHL in AAH25835 (PubMed:15489334).Curated | 3 | |
Sequence conflicti | 1113 | R → G in BAC98278 (PubMed:14621295).Curated | 1 | |
Sequence conflicti | 1113 | R → G in AAH25835 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1166 | P → S in BAC98278 (PubMed:14621295).Curated | 1 | |
Sequence conflicti | 1166 | P → S in AAH25835 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1211 | P → L in BAC98278 (PubMed:14621295).Curated | 1 | |
Sequence conflicti | 1211 | P → L in AAH25835 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_025941 | 205 – 353 | Missing in isoform 4. 1 PublicationAdd BLAST | 149 | |
Alternative sequenceiVSP_025942 | 512 – 530 | WPFPR…GVATP → ELGKEPGLLSTPLLMEGEP in isoform 2. 1 PublicationAdd BLAST | 19 | |
Alternative sequenceiVSP_025943 | 531 – 1404 | Missing in isoform 2. 1 PublicationAdd BLAST | 874 | |
Alternative sequenceiVSP_025944 | 1226 – 1244 | SAVSR…DTLRC → KRQHFVQWRLKPLPTEKFY in isoform 3 and isoform 4. 2 PublicationsAdd BLAST | 19 | |
Alternative sequenceiVSP_025945 | 1245 – 1404 | Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST | 160 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK129468 mRNA Translation: BAC98278.1 Different initiation. AK154663 mRNA Translation: BAE32751.1 AK170121 mRNA Translation: BAE41578.1 AK170885 mRNA Translation: BAE42093.1 AL683828 Genomic DNA No translation available. BC025835 mRNA Translation: AAH25835.1 BC036324 mRNA Translation: AAH36324.1 BC092227 mRNA Translation: AAH92227.1 |
CCDSi | CCDS38777.1 [Q80VW7-1] |
RefSeqi | NP_001038979.1, NM_001045514.3 [Q80VW7-1] NP_001292361.1, NM_001305432.1 [Q80VW7-1] XP_006537583.1, XM_006537520.3 [Q80VW7-1] XP_006537584.1, XM_006537521.2 [Q80VW7-1] XP_006537585.1, XM_006537522.3 [Q80VW7-1] |
Genome annotation databases
Ensembli | ENSMUST00000035724; ENSMUSP00000041614; ENSMUSG00000039158 [Q80VW7-1] |
GeneIDi | 100182 |
KEGGi | mmu:100182 |
UCSCi | uc008tgb.3, mouse [Q80VW7-1] uc008tge.2, mouse [Q80VW7-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK129468 mRNA Translation: BAC98278.1 Different initiation. AK154663 mRNA Translation: BAE32751.1 AK170121 mRNA Translation: BAE41578.1 AK170885 mRNA Translation: BAE42093.1 AL683828 Genomic DNA No translation available. BC025835 mRNA Translation: AAH25835.1 BC036324 mRNA Translation: AAH36324.1 BC092227 mRNA Translation: AAH92227.1 |
CCDSi | CCDS38777.1 [Q80VW7-1] |
RefSeqi | NP_001038979.1, NM_001045514.3 [Q80VW7-1] NP_001292361.1, NM_001305432.1 [Q80VW7-1] XP_006537583.1, XM_006537520.3 [Q80VW7-1] XP_006537584.1, XM_006537521.2 [Q80VW7-1] XP_006537585.1, XM_006537522.3 [Q80VW7-1] |
3D structure databases
AlphaFoldDBi | Q80VW7 |
SMRi | Q80VW7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000041614 |
PTM databases
iPTMneti | Q80VW7 |
PhosphoSitePlusi | Q80VW7 |
Proteomic databases
EPDi | Q80VW7 |
jPOSTi | Q80VW7 |
MaxQBi | Q80VW7 |
PaxDbi | Q80VW7 |
PeptideAtlasi | Q80VW7 |
PRIDEi | Q80VW7 |
ProteomicsDBi | 296154 [Q80VW7-1] 296155 [Q80VW7-2] 296156 [Q80VW7-3] 296157 [Q80VW7-4] |
Protocols and materials databases
Antibodypediai | 54667, 41 antibodies from 14 providers |
Genome annotation databases
Ensembli | ENSMUST00000035724; ENSMUSP00000041614; ENSMUSG00000039158 [Q80VW7-1] |
GeneIDi | 100182 |
KEGGi | mmu:100182 |
UCSCi | uc008tgb.3, mouse [Q80VW7-1] uc008tge.2, mouse [Q80VW7-2] |
Organism-specific databases
CTDi | 80709 |
MGIi | MGI:2140340, Akna |
VEuPathDBi | HostDB:ENSMUSG00000039158 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | ENOG502QRSN, Eukaryota |
GeneTreei | ENSGT00940000154254 |
HOGENOMi | CLU_005641_0_0_1 |
InParanoidi | Q80VW7 |
OMAi | GTEVHWA |
OrthoDBi | 112521at2759 |
PhylomeDBi | Q80VW7 |
TreeFami | TF336885 |
Miscellaneous databases
BioGRID-ORCSi | 100182, 2 hits in 57 CRISPR screens |
PROi | PR:Q80VW7 |
RNActi | Q80VW7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039158, Expressed in granulocyte and 256 other tissues |
Genevisiblei | Q80VW7, MM |
Family and domain databases
InterProi | View protein in InterPro IPR022150, AKNA_dom |
Pfami | View protein in Pfam PF12443, AKNA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AKNA_MOUSE | |
Accessioni | Q80VW7Primary (citable) accession number: Q80VW7 Secondary accession number(s): Q3TC64 Q8R114 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2007 |
Last sequence update: | June 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families