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Protein

Epsin-1

Gene

Epn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei25Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei30Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei63Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei73Phosphatidylinositol lipid headgroupBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-182971 EGFR downregulation
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Epsin-1
Alternative name(s):
EPS-15-interacting protein 1
Intersectin-EH-binding protein 1
Short name:
Ibp1
Gene namesi
Name:Epn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1333763 Epn1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Coated pit, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745141 – 575Epsin-1Add BLAST575

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei382PhosphoserineBy similarity1
Modified residuei418PhosphoserineCombined sources1
Modified residuei419PhosphoserineCombined sources1
Modified residuei420PhosphothreonineCombined sources1
Modified residuei434PhosphoserineBy similarity1
Modified residuei446PhosphoserineBy similarity1
Modified residuei453PhosphoserineBy similarity1
Modified residuei459PhosphothreonineCombined sources1
Modified residuei463PhosphothreonineBy similarity1
Modified residuei469PhosphothreonineCombined sources1
Modified residuei472PhosphoserineCombined sources1
Modified residuei493PhosphothreonineCombined sources1
Modified residuei533Omega-N-methylarginineCombined sources1

Post-translational modificationi

Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.1 Publication
Ubiquitinated.By similarity

Keywords - PTMi

Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ80VP1
PaxDbiQ80VP1
PeptideAtlasiQ80VP1
PRIDEiQ80VP1

PTM databases

iPTMnetiQ80VP1
PhosphoSitePlusiQ80VP1

Expressioni

Gene expression databases

BgeeiENSMUSG00000035203 Expressed in 266 organ(s), highest expression level in CA1 field of hippocampus
CleanExiMM_EPN1
ExpressionAtlasiQ80VP1 baseline and differential
GenevisibleiQ80VP1 MM

Interactioni

Subunit structurei

Monomer. Binds clathrin, ZBTB16/ZNF145, REPS2 and EPS15 (By similarity). Binds ubiquitinated proteins (By similarity). Interacts with RALBP1 in a complex also containing NUMB and TFAP2A during interphase and mitosis (By similarity). Interacts with AP2B1 (By similarity). Interacts with UBQLN2 (By similarity). Interacts with ITSN1. Interacts with AP2A1 and AP2A2.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199485, 4 interactors
DIPiDIP-43943N
ELMiQ80VP1
IntActiQ80VP1, 6 interactors
MINTiQ80VP1
STRINGi10090.ENSMUSP00000043340

Structurei

3D structure databases

ProteinModelPortaliQ80VP1
SMRiQ80VP1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 144ENTHPROSITE-ProRule annotationAdd BLAST133
Domaini183 – 202UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini208 – 227UIM 2PROSITE-ProRule annotationAdd BLAST20
Domaini233 – 252UIM 3PROSITE-ProRule annotationAdd BLAST20
Repeati274 – 27613
Repeati294 – 29623
Repeati306 – 30833
Repeati319 – 32143
Repeati332 – 33453
Repeati349 – 35163
Repeati367 – 36973
Repeati377 – 37983
Repeati501 – 50313
Repeati517 – 51923
Repeati571 – 57333

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni274 – 3798 X 3 AA repeats of D-P-WAdd BLAST106
Regioni501 – 5733 X 3 AA repeats of N-P-FAdd BLAST73

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi401 – 410[DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi267 – 572Ala/Gly/Pro-richAdd BLAST306

Domaini

The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP2A2 and clathrin binding.
The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction the AP-2 complex subunit AP2B1.By similarity

Sequence similaritiesi

Belongs to the epsin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2056 Eukaryota
ENOG410XSM0 LUCA
GeneTreeiENSGT00550000074611
HOGENOMiHOG000008298
HOVERGENiHBG006690
InParanoidiQ80VP1
KOiK12471
OMAiITNPFQP
OrthoDBiEOG091G0L45
PhylomeDBiQ80VP1
TreeFamiTF313361

Family and domain databases

CDDicd03571 ENTH_epsin, 1 hit
Gene3Di1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR013809 ENTH
IPR008942 ENTH_VHS
IPR039416 Epsin_ENTH
IPR003903 UIM_dom
PfamiView protein in Pfam
PF01417 ENTH, 1 hit
PF02809 UIM, 2 hits
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SM00726 UIM, 3 hits
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit
PS50330 UIM, 3 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q80VP1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSTSSLRRQM KNIVHNYSEA EIKVREATSN DPWGPSSSLM SEIADLTYNV
60 70 80 90 100
VAFSEIMSMI WKRLNDHGKN WRHVYKAMTL MEYLIKTGSE RVSQQCKENM
110 120 130 140 150
YAVQTLKDFQ YVDRDGKDQG VNVREKAKQL VALLRDEDRL REERAHALKT
160 170 180 190 200
KEKLAQTATA SSAAVGSGPP PEAEQAWPQS SGEEELQLQL ALAMSKEEAD
210 220 230 240 250
QPPSCGPEDD VQLQLALSLS REEHDKEERI RRGDDLRLQM AIEESKRETG
260 270 280 290 300
GKEESSLMDL ADVFTTPAPP QASDPWGGPA SVPTAVPVAA AASDPWGGPA
310 320 330 340 350
VPPAADPWGG AAPTPASGDP WRPAAPTGPS VDPWGGTPAP AAGEGPTPDP
360 370 380 390 400
WGSSDGGAPV SGPPSSDPWA PAPAFSDPWG GSPAKPSSNG TAVGGFDTEP
410 420 430 440 450
DEFSDFDRLR TALPTSGSST GELELLAGEV PARSPGAFDM SGVGGSLAES
460 470 480 490 500
VGSPPPAATP TPTPPTRKTP ESFLGPNAAL VDLDSLVSRP GPTPPGSKAS
510 520 530 540 550
NPFLPSGAPP TGPSVTNPFQ PAPPATLTLN QLRLSPVPPV PGAPPTYISP
560 570
LGGGPGLPPM MPPGPPAPNT NPFLL
Length:575
Mass (Da):60,212
Last modified:November 28, 2006 - v3
Checksum:i70B8011EB3AE5C4C
GO
Isoform 2 (identifier: Q80VP1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     392-392: A → AA

Note: No experimental confirmation available.
Show »
Length:576
Mass (Da):60,283
Checksum:i162775BC51E4E88A
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z4V3D3Z4V3_MOUSE
Epsin-1
Epn1
90Annotation score:
D3Z550D3Z550_MOUSE
Epsin-1
Epn1
54Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009153392A → AA in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC046962 mRNA Translation: AAH46962.2
BC067206 mRNA Translation: AAH67206.1
BC099682 mRNA Translation: AAH99682.1
AF057285 mRNA Translation: AAC97475.1
CCDSiCCDS20758.1 [Q80VP1-1]
RefSeqiNP_001239383.1, NM_001252454.1 [Q80VP1-1]
NP_034277.1, NM_010147.4 [Q80VP1-1]
XP_006539592.2, XM_006539529.3 [Q80VP1-2]
XP_006539594.2, XM_006539531.3 [Q80VP1-2]
XP_006539596.1, XM_006539533.1 [Q80VP1-2]
XP_006539597.1, XM_006539534.1 [Q80VP1-2]
XP_006539598.1, XM_006539535.1 [Q80VP1-2]
XP_006539599.1, XM_006539536.1 [Q80VP1-2]
UniGeneiMm.3091

Genome annotation databases

EnsembliENSMUST00000045277; ENSMUSP00000043340; ENSMUSG00000035203 [Q80VP1-1]
ENSMUST00000098845; ENSMUSP00000096445; ENSMUSG00000035203 [Q80VP1-1]
ENSMUST00000208634; ENSMUSP00000146638; ENSMUSG00000035203 [Q80VP1-2]
GeneIDi13854
KEGGimmu:13854
UCSCiuc009ezx.2 mouse [Q80VP1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Protein Spotlight

The bubble's bend - Issue 42 of January 2004

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC046962 mRNA Translation: AAH46962.2
BC067206 mRNA Translation: AAH67206.1
BC099682 mRNA Translation: AAH99682.1
AF057285 mRNA Translation: AAC97475.1
CCDSiCCDS20758.1 [Q80VP1-1]
RefSeqiNP_001239383.1, NM_001252454.1 [Q80VP1-1]
NP_034277.1, NM_010147.4 [Q80VP1-1]
XP_006539592.2, XM_006539529.3 [Q80VP1-2]
XP_006539594.2, XM_006539531.3 [Q80VP1-2]
XP_006539596.1, XM_006539533.1 [Q80VP1-2]
XP_006539597.1, XM_006539534.1 [Q80VP1-2]
XP_006539598.1, XM_006539535.1 [Q80VP1-2]
XP_006539599.1, XM_006539536.1 [Q80VP1-2]
UniGeneiMm.3091

3D structure databases

ProteinModelPortaliQ80VP1
SMRiQ80VP1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199485, 4 interactors
DIPiDIP-43943N
ELMiQ80VP1
IntActiQ80VP1, 6 interactors
MINTiQ80VP1
STRINGi10090.ENSMUSP00000043340

PTM databases

iPTMnetiQ80VP1
PhosphoSitePlusiQ80VP1

Proteomic databases

EPDiQ80VP1
PaxDbiQ80VP1
PeptideAtlasiQ80VP1
PRIDEiQ80VP1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045277; ENSMUSP00000043340; ENSMUSG00000035203 [Q80VP1-1]
ENSMUST00000098845; ENSMUSP00000096445; ENSMUSG00000035203 [Q80VP1-1]
ENSMUST00000208634; ENSMUSP00000146638; ENSMUSG00000035203 [Q80VP1-2]
GeneIDi13854
KEGGimmu:13854
UCSCiuc009ezx.2 mouse [Q80VP1-1]

Organism-specific databases

CTDi29924
MGIiMGI:1333763 Epn1

Phylogenomic databases

eggNOGiKOG2056 Eukaryota
ENOG410XSM0 LUCA
GeneTreeiENSGT00550000074611
HOGENOMiHOG000008298
HOVERGENiHBG006690
InParanoidiQ80VP1
KOiK12471
OMAiITNPFQP
OrthoDBiEOG091G0L45
PhylomeDBiQ80VP1
TreeFamiTF313361

Enzyme and pathway databases

ReactomeiR-MMU-182971 EGFR downregulation
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

ChiTaRSiEpn1 mouse
PROiPR:Q80VP1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035203 Expressed in 266 organ(s), highest expression level in CA1 field of hippocampus
CleanExiMM_EPN1
ExpressionAtlasiQ80VP1 baseline and differential
GenevisibleiQ80VP1 MM

Family and domain databases

CDDicd03571 ENTH_epsin, 1 hit
Gene3Di1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR013809 ENTH
IPR008942 ENTH_VHS
IPR039416 Epsin_ENTH
IPR003903 UIM_dom
PfamiView protein in Pfam
PF01417 ENTH, 1 hit
PF02809 UIM, 2 hits
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SM00726 UIM, 3 hits
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit
PS50330 UIM, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiEPN1_MOUSE
AccessioniPrimary (citable) accession number: Q80VP1
Secondary accession number(s): O70446, Q6NX78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 28, 2006
Last modified: November 7, 2018
This is version 141 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
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