UniProtKB - Q80VL1 (TDRKH_MOUSE)
Protein
Tudor and KH domain-containing protein
Gene
Tdrkh
Organism
Mus musculus (Mouse)
Status
Functioni
Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.1 Publication
GO - Molecular functioni
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- DNA methylation involved in gamete generation Source: UniProtKB
- fertilization Source: UniProtKB
- gene silencing by RNA Source: UniProtKB-KW
- male meiotic nuclear division Source: UniProtKB
- piRNA metabolic process Source: UniProtKB
- spermatogenesis Source: UniProtKB
Keywordsi
Molecular function | RNA-binding |
Biological process | Differentiation, RNA-mediated gene silencing, Spermatogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: Tudor and KH domain-containing proteinAlternative name(s): Tudor domain-containing protein 2 |
Gene namesi | Name:Tdrkh Synonyms:Tdrd2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919884, Tdrkh |
Subcellular locationi
Mitochondrion
Other locations
Note: Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Colocalizes with pi- and piP-bodies, a subset of the nuage which contains secondary piRNAs. Associated with mitochondria in the germline.
Cytosol
- cytosol Source: Reactome
Mitochondrion
- mitochondrion Source: UniProtKB
Other locations
Keywords - Cellular componenti
Cytoplasm, MitochondrionPathology & Biotechi
Disruption phenotypei
Male mice are sterile due to defects in male meiosis, piRNA production defects, DNA demethylation of LINE-1 (L1) transposable elements and an increase in L1 expression in the adult testis. Mutants have severely reduced levels of mature piRNAs and accumulate 1'U-containing, 2'O-methylated 31-37 nt RNAs that complement the missing mature piRNAs.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050142 | 1 – 560 | Tudor and KH domain-containing proteinAdd BLAST | 560 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 65 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 76 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 110 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 112 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 152 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 175 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 181 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 187 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 193 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 256 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 267 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 278 | PhosphoserineBy similarity | 1 | |
Cross-linki | 479 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 510 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 529 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity |
Post-translational modificationi
Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.By similarity
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q80VL1 |
MaxQBi | Q80VL1 |
PaxDbi | Q80VL1 |
PRIDEi | Q80VL1 |
PTM databases
iPTMneti | Q80VL1 |
PhosphoSitePlusi | Q80VL1 |
SwissPalmi | Q80VL1 |
Expressioni
Tissue specificityi
Highly expressed in testis, present at lower level in brain. Weakly or not expressed in other tissues (at protein level).1 Publication
Developmental stagei
Present at low level in male gonads in postnatal day 7 (P7) Expressed at higher level in P14, P21 and adult testes, correlating with the onset of meiosis (at protein level). Expressed in spermatogonia, spermatocytes and round spermatids, but not in elongating spermatids.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000041912, Expressed in primary oocyte and 200 other tissues |
Genevisiblei | Q80VL1, MM |
Interactioni
Subunit structurei
Interacts with (symmetrically methylated) PIWIL1, PIWIL2 and PIWIL4.
3 PublicationsProtein-protein interaction databases
IntActi | Q80VL1, 1 interactor |
MINTi | Q80VL1 |
STRINGi | 10090.ENSMUSP00000129635 |
Miscellaneous databases
RNActi | Q80VL1, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q80VL1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q80VL1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 52 – 115 | KH 1PROSITE-ProRule annotationAdd BLAST | 64 | |
Domaini | 124 – 190 | KH 2PROSITE-ProRule annotationAdd BLAST | 67 | |
Domaini | 353 – 412 | TudorPROSITE-ProRule annotationAdd BLAST | 60 |
Sequence similaritiesi
Belongs to the Tdrkh family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2279, Eukaryota |
GeneTreei | ENSGT00940000159364 |
HOGENOMi | CLU_023629_1_0_1 |
InParanoidi | Q80VL1 |
OMAi | MEVYVSA |
OrthoDBi | 971207at2759 |
PhylomeDBi | Q80VL1 |
TreeFami | TF318292 |
Family and domain databases
Gene3Di | 2.40.50.90, 1 hit 3.30.1370.10, 2 hits |
InterProi | View protein in InterPro IPR004087, KH_dom IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf IPR035437, SNase_OB-fold_sf IPR002999, Tudor |
Pfami | View protein in Pfam PF00013, KH_1, 2 hits PF00567, TUDOR, 1 hit |
SMARTi | View protein in SMART SM00322, KH, 2 hits SM00333, TUDOR, 1 hit |
SUPFAMi | SSF54791, SSF54791, 2 hits |
PROSITEi | View protein in PROSITE PS50084, KH_TYPE_1, 2 hits PS50304, TUDOR, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
Q80VL1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTERTSWTN LSTIQKIALG LGIPASATVA YILYRRYRES REERLTFVGE
60 70 80 90 100
DDIEIEMRVP QEAVKLIIGR QGANIKQLRK QTGARIDVDT EDVGDERVLL
110 120 130 140 150
ISGFPVQVCK AKAAIHQILT ENTPVFEQLS VPQRSVGRII GRGGETIRSI
160 170 180 190 200
CKASGAKITC DKESEGTLLL SRLIKISGTQ KEVAAAKHLI LEKVSEDEEL
210 220 230 240 250
RKRIAHSAET RVPRKQPISV RREEVTEPGG AGEAALWKNT NSSMGPATPL
260 270 280 290 300
EVPLRKGGGD MVVVGPKEGS WEKPNDDSFQ NSGAQSSPET SMFEIPSPDF
310 320 330 340 350
SFHADEYLEV YVSASEHPNH FWIQIIGSRS LQLDKLVSEM TQHYENSLPE
360 370 380 390 400
DLTVHVGDIV AAPLSTNGSW YRARVLGTLE NGNLDLYFVD FGDNGDCALK
410 420 430 440 450
DLRALRSDFL SLPFQAIECS LARIAPTGEE WEEEALDEFD RLTHCADWKP
460 470 480 490 500
LVAKISSYVQ TGISTWPKIY LYDTSDEKKL DIGLELVRKG YAVELPEDME
510 520 530 540 550
ENRTVPNMLK DMATETDDSL ASILTETKKS PEEMPHTLSC LSLSEAASMS
560
GDDNLEDDLF
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JFB2 | A0A0G2JFB2_MOUSE | Tudor and KH domain-containing prot... | Tdrkh | 556 | Annotation score: | ||
A0A0G2JFE0 | A0A0G2JFE0_MOUSE | Tudor and KH domain-containing prot... | Tdrkh | 515 | Annotation score: | ||
A0A0G2JE08 | A0A0G2JE08_MOUSE | Tudor and KH domain-containing prot... | Tdrkh | 296 | Annotation score: | ||
A0A0G2JFP5 | A0A0G2JFP5_MOUSE | Tudor and KH domain-containing prot... | Tdrkh | 140 | Annotation score: | ||
A0A0G2JEH9 | A0A0G2JEH9_MOUSE | Tudor and KH domain-containing prot... | Tdrkh | 50 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GL456099 Genomic DNA No translation available. BC049363 mRNA Translation: AAH49363.1 |
CCDSi | CCDS38534.1 |
RefSeqi | NP_082583.1, NM_028307.1 XP_006502201.1, XM_006502138.3 XP_017175235.1, XM_017319746.1 |
Genome annotation databases
Ensembli | ENSMUST00000045245; ENSMUSP00000041002; ENSMUSG00000041912 ENSMUST00000166032; ENSMUSP00000129635; ENSMUSG00000041912 ENSMUST00000197901; ENSMUSP00000142561; ENSMUSG00000041912 |
GeneIDi | 72634 |
KEGGi | mmu:72634 |
UCSCi | uc008qge.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GL456099 Genomic DNA No translation available. BC049363 mRNA Translation: AAH49363.1 |
CCDSi | CCDS38534.1 |
RefSeqi | NP_082583.1, NM_028307.1 XP_006502201.1, XM_006502138.3 XP_017175235.1, XM_017319746.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WE8 | NMR | - | A | 118-208 | [»] | |
SMRi | Q80VL1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | Q80VL1, 1 interactor |
MINTi | Q80VL1 |
STRINGi | 10090.ENSMUSP00000129635 |
PTM databases
iPTMneti | Q80VL1 |
PhosphoSitePlusi | Q80VL1 |
SwissPalmi | Q80VL1 |
Proteomic databases
EPDi | Q80VL1 |
MaxQBi | Q80VL1 |
PaxDbi | Q80VL1 |
PRIDEi | Q80VL1 |
Protocols and materials databases
Antibodypediai | 10583, 69 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000045245; ENSMUSP00000041002; ENSMUSG00000041912 ENSMUST00000166032; ENSMUSP00000129635; ENSMUSG00000041912 ENSMUST00000197901; ENSMUSP00000142561; ENSMUSG00000041912 |
GeneIDi | 72634 |
KEGGi | mmu:72634 |
UCSCi | uc008qge.1, mouse |
Organism-specific databases
CTDi | 11022 |
MGIi | MGI:1919884, Tdrkh |
Phylogenomic databases
eggNOGi | KOG2279, Eukaryota |
GeneTreei | ENSGT00940000159364 |
HOGENOMi | CLU_023629_1_0_1 |
InParanoidi | Q80VL1 |
OMAi | MEVYVSA |
OrthoDBi | 971207at2759 |
PhylomeDBi | Q80VL1 |
TreeFami | TF318292 |
Miscellaneous databases
BioGRID-ORCSi | 72634, 2 hits in 19 CRISPR screens |
ChiTaRSi | Tdrkh, mouse |
EvolutionaryTracei | Q80VL1 |
PROi | PR:Q80VL1 |
RNActi | Q80VL1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000041912, Expressed in primary oocyte and 200 other tissues |
Genevisiblei | Q80VL1, MM |
Family and domain databases
Gene3Di | 2.40.50.90, 1 hit 3.30.1370.10, 2 hits |
InterProi | View protein in InterPro IPR004087, KH_dom IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf IPR035437, SNase_OB-fold_sf IPR002999, Tudor |
Pfami | View protein in Pfam PF00013, KH_1, 2 hits PF00567, TUDOR, 1 hit |
SMARTi | View protein in SMART SM00322, KH, 2 hits SM00333, TUDOR, 1 hit |
SUPFAMi | SSF54791, SSF54791, 2 hits |
PROSITEi | View protein in PROSITE PS50084, KH_TYPE_1, 2 hits PS50304, TUDOR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TDRKH_MOUSE | |
Accessioni | Q80VL1Primary (citable) accession number: Q80VL1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2004 |
Last sequence update: | June 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families