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Protein

AP-4 complex subunit epsilon-1

Gene

Ap4e1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways. AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons. AP-4 is involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos, but may also recognize other types of sorting signal.By similarity

GO - Biological processi

  • intracellular protein transport Source: MGI
  • vesicle-mediated transport Source: MGI

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-432720 Lysosome Vesicle Biogenesis
R-MMU-432722 Golgi Associated Vesicle Biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
AP-4 complex subunit epsilon-1Curated
Alternative name(s):
AP-4 adaptor complex subunit epsilon
Adaptor-related protein complex 4 subunit epsilon-1
Epsilon subunit of AP-4
Epsilon-adaptin
Gene namesi
Name:Ap4e1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1336993 Ap4e1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002297491 – 1122AP-4 complex subunit epsilon-1CuratedAdd BLAST1122

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei699PhosphoserineBy similarity1
Modified residuei851PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ80V94
MaxQBiQ80V94
PaxDbiQ80V94
PeptideAtlasiQ80V94
PRIDEiQ80V94

PTM databases

iPTMnetiQ80V94
PhosphoSitePlusiQ80V94

Expressioni

Gene expression databases

BgeeiENSMUSG00000001998 Expressed in 233 organ(s), highest expression level in quadriceps femoris
CleanExiMM_AP4E1
ExpressionAtlasiQ80V94 baseline and differential
GenevisibleiQ80V94 MM

Interactioni

Subunit structurei

Adaptor protein complex 4 (AP-4) is a heterotetramer composed of two large adaptins (epsilon-type subunit AP4E1 and beta-type subunit AP4B1), a medium adaptin (mu-type subunit AP4M1) and a small adaptin (sigma-type AP4S1). Interacts with TEPSIN (By similarity). Interacts with GRIA2; probably indirect it mediates the somatodendritic localization of GRIA2 in neurons (PubMed:18341993).By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000002063

Structurei

3D structure databases

ProteinModelPortaliQ80V94
SMRiQ80V94
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni726 – 1122Interaction with TEPSINBy similarityAdd BLAST397

Sequence similaritiesi

Belongs to the adaptor complexes large subunit family.Sequence analysis

Phylogenomic databases

eggNOGiKOG1062 Eukaryota
ENOG410XPKK LUCA
GeneTreeiENSGT00390000012618
HOGENOMiHOG000231886
HOVERGENiHBG050522
InParanoidiQ80V94
KOiK12400
OMAiSYKIWKD
OrthoDBiEOG091G010Y
TreeFamiTF332488

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR017109 AP4_complex_esu
IPR028269 AP4E1_C
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR002553 Clathrin/coatomer_adapt-like_N
PfamiView protein in Pfam
PF01602 Adaptin_N, 1 hit
PF14807 AP4E_app_platf, 1 hit
PIRSFiPIRSF037097 AP4_complex_epsilon, 1 hit
SMARTiView protein in SMART
SM01356 AP4E_app_platf, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

Q80V94-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDMVERTLT ALPGLFLQNQ LGGPAASRAP FFSRLGGLIR GVTALSSKHE
60 70 80 90 100
EEKLIQQELS SLKATVSAPT TTLKTMKECM VRLIYCEMLG YDASFGYIHA
110 120 130 140 150
IKLAQQGNLL EKRVGYLAVS LFLHESHELL LLLVNTVVKD LQSTNLVEVC
160 170 180 190 200
MALTVVSQIF PREMIPAVLP LIEDKLQHSK EIIRRKAVLA LYKFYLIAPN
210 220 230 240 250
QVQHIHTKFR KALCDRDVGV MAASLHIYLR MIKENASGYK DLTESFVTIL
260 270 280 290 300
KQVVGGKLPV EFSYHSVPAP WLQIQLLRIL GLLGKDDERT SELMYDVLDE
310 320 330 340 350
SLRRAELNHN VTYAILFECV HTIYSIYPKS ELLEKAAKCI GKFVLSPKIN
360 370 380 390 400
LKYLGLKALT YVIQQDPSLA LQHQITIIEC LDHPDPIIKR ETLELLYRIT
410 420 430 440 450
NAQNVVVIVQ KMLEYLHQSK EEHIIISLVG RIAELAEKYA PDNVWFIQTM
460 470 480 490 500
NAVFSVGGDV MHPDILSNFL RLLAEGFDDE TEDQQLRLYA VQSYLTLLDM
510 520 530 540 550
ENTFYPQRFL QVMSWVLGEY SYLLDKESPE EVITRLYKLL MSDSISSETK
560 570 580 590 600
AWLFAAVTKL TPQAHSSPLV EKLIQEFTVS LNTCLRQHAF ELKHLHENTE
610 620 630 640 650
LMKSLLQGAQ NCEDIVADAS LSFLDGFVAE GLSQGAAPYK PHHQRQEEQL
660 670 680 690 700
SQEKVLNFEP YGLSFSSSGF TGRQSPAGIS LGSDISGNSA ETGLKETSSL
710 720 730 740 750
KMEGIKKLWG KEGYLPKKES GTGDKPEASH VPAEGATVEN VDQATTRKDQ
760 770 780 790 800
AQGHIPSTEE KEKQLLASSL FVGLGPENTV DLLGKADVVS HKFRRKSKLK
810 820 830 840 850
VAQSDKTPSA PTAPCSALSL GSDVAGGDED GLSAVDRGDG ELSSELFRSE
860 870 880 890 900
SLSGPPSAEK LESVSLPVPS LFADNNMEVF NPPSSSATST VKEETPECRH
910 920 930 940 950
SGLVEICSNE AVSVSSYKVW RDDCLLVIWA VTSKTDSEFT DAQLEIFPVE
960 970 980 990 1000
NFKIIEQPEC SSPVIETERT KSFQYSVQME SPCIEGTLSG FIKYQMMDTH
1010 1020 1030 1040 1050
SVQLEFSMNL PLLDFIRPLK ISTEDFGKLW LSFANDVKQT IKISEPGVAL
1060 1070 1080 1090 1100
TSVLTELQQN LRLRVIDVIG NEGLLACKLL PSTPCVLHCR VHADAVALWF
1110 1120
RSSSSVLSDY LSCHCQKVMQ TS
Length:1,122
Mass (Da):124,845
Last modified:July 27, 2011 - v3
Checksum:iD5B4E518714DBA3D
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2ASB3A2ASB3_MOUSE
AP-4 complex subunit epsilon-1
Ap4e1
393Annotation score:
H3BL03H3BL03_MOUSE
AP-4 complex subunit epsilon-1
Ap4e1
367Annotation score:
H3BKM4H3BKM4_MOUSE
AP-4 complex subunit epsilon-1
Ap4e1
292Annotation score:
H3BK54H3BK54_MOUSE
AP-4 complex subunit epsilon-1
Ap4e1
107Annotation score:
F6W7A9F6W7A9_MOUSE
AP-4 complex subunit epsilon-1
Ap4e1
50Annotation score:

Sequence cautioni

The sequence AK144636 differs from that shown. Reason: Frameshift at position 303.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti338K → Q in AK144636 (PubMed:16141072).Curated1
Sequence conflicti379E → G in AK144636 (PubMed:16141072).Curated1
Sequence conflicti936D → E in AK144636 (PubMed:16141072).Curated1
Sequence conflicti954I → M in AK144636 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK144636 mRNA No translation available.
AL928836, AL928590 Genomic DNA Translation: CAM18335.1
AL928590, AL928836 Genomic DNA Translation: CAM19126.1
BC042530 mRNA Translation: AAH42530.1
CCDSiCCDS38231.1
RefSeqiNP_780759.2, NM_175550.3
UniGeneiMm.134045

Genome annotation databases

EnsembliENSMUST00000002063; ENSMUSP00000002063; ENSMUSG00000001998
GeneIDi108011
KEGGimmu:108011
UCSCiuc008men.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK144636 mRNA No translation available.
AL928836, AL928590 Genomic DNA Translation: CAM18335.1
AL928590, AL928836 Genomic DNA Translation: CAM19126.1
BC042530 mRNA Translation: AAH42530.1
CCDSiCCDS38231.1
RefSeqiNP_780759.2, NM_175550.3
UniGeneiMm.134045

3D structure databases

ProteinModelPortaliQ80V94
SMRiQ80V94
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000002063

PTM databases

iPTMnetiQ80V94
PhosphoSitePlusiQ80V94

Proteomic databases

EPDiQ80V94
MaxQBiQ80V94
PaxDbiQ80V94
PeptideAtlasiQ80V94
PRIDEiQ80V94

Protocols and materials databases

DNASUi108011
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002063; ENSMUSP00000002063; ENSMUSG00000001998
GeneIDi108011
KEGGimmu:108011
UCSCiuc008men.1 mouse

Organism-specific databases

CTDi23431
MGIiMGI:1336993 Ap4e1

Phylogenomic databases

eggNOGiKOG1062 Eukaryota
ENOG410XPKK LUCA
GeneTreeiENSGT00390000012618
HOGENOMiHOG000231886
HOVERGENiHBG050522
InParanoidiQ80V94
KOiK12400
OMAiSYKIWKD
OrthoDBiEOG091G010Y
TreeFamiTF332488

Enzyme and pathway databases

ReactomeiR-MMU-432720 Lysosome Vesicle Biogenesis
R-MMU-432722 Golgi Associated Vesicle Biogenesis

Miscellaneous databases

PROiPR:Q80V94
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001998 Expressed in 233 organ(s), highest expression level in quadriceps femoris
CleanExiMM_AP4E1
ExpressionAtlasiQ80V94 baseline and differential
GenevisibleiQ80V94 MM

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR017109 AP4_complex_esu
IPR028269 AP4E1_C
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR002553 Clathrin/coatomer_adapt-like_N
PfamiView protein in Pfam
PF01602 Adaptin_N, 1 hit
PF14807 AP4E_app_platf, 1 hit
PIRSFiPIRSF037097 AP4_complex_epsilon, 1 hit
SMARTiView protein in SMART
SM01356 AP4E_app_platf, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAP4E1_MOUSE
AccessioniPrimary (citable) accession number: Q80V94
Secondary accession number(s): A2ASB4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 117 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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