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Protein

Carbohydrate sulfotransferase 14

Gene

Chst14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of dermatan sulfate. Plays a pivotal role in the formation of 4-0-sulfated IdoA blocks in dermatan sulfate. Transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. Transfers sulfate more efficiently to GalNAc residues in -IdoUA-GalNAc-IdoUA- than in -GlcUA-GalNAc-GlcUA-sequences. Has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA. Appears to have an important role in the formation of the cerebellar neural network during postnatal brain development.1 Publication

Catalytic activityi

3'-phospho-5'-adenylyl sulfate + [dermatan]-N-acetyl-D-galactosamine = adenosine 3',5'-bisphosphate + [dermatan]- 4-O-sulfo-N-acetyl-D-galactosamine.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi155 – 161PAPSBy similarity7
Nucleotide bindingi213 – 221PAPSBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

BRENDAi2.8.2.35 3474
ReactomeiR-MMU-2022923 Dermatan sulfate biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 14 (EC:2.8.2.35)
Alternative name(s):
Dermatan 4-sulfotransferase 1
Short name:
D4ST-1
Gene namesi
Name:Chst14
Synonyms:D4st1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1919386 Chst14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 39CytoplasmicSequence analysisAdd BLAST39
Transmembranei40 – 60Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini61 – 376LumenalSequence analysisAdd BLAST316

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001896731 – 376Carbohydrate sulfotransferase 14Add BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi110N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi368N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ80V53
PaxDbiQ80V53
PeptideAtlasiQ80V53
PRIDEiQ80V53

PTM databases

PhosphoSitePlusiQ80V53

Expressioni

Developmental stagei

Low levels of expression in olfactory bulb, caudate putamen, cerebral cortex, hippocampus, thalamus, midbrain and cerebellum during early postnatal development. In later stages, exclusively expressed in cerebellum culminating at P14 of postnatal development.1 Publication

Gene expression databases

BgeeiENSMUSG00000074916 Expressed in 209 organ(s), highest expression level in humerus cartilage element
ExpressionAtlasiQ80V53 baseline and differential
GenevisibleiQ80V53 MM

Interactioni

Protein-protein interaction databases

IntActiQ80V53, 2 interactors
MINTiQ80V53
STRINGi10090.ENSMUSP00000099579

Structurei

3D structure databases

ProteinModelPortaliQ80V53
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfotransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4651 Eukaryota
ENOG4111GJR LUCA
GeneTreeiENSGT00760000119214
HOGENOMiHOG000231801
HOVERGENiHBG050954
InParanoidiQ80V53
KOiK08105
OMAiRYRLQHY
OrthoDBiEOG091G0FZO
PhylomeDBiQ80V53
TreeFamiTF325581

Family and domain databases

InterProiView protein in InterPro
IPR018011 Carb_sulfotrans_8-10
IPR005331 Sulfotransferase
PANTHERiPTHR12137 PTHR12137, 1 hit
PfamiView protein in Pfam
PF03567 Sulfotransfer_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q80V53-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFPRPLTPLA APKSAETLGR TPRRAPLGRA RAGLGGPPLL LPSMLMFAVI
60 70 80 90 100
VASSGLLLMI ERGILSEMKP LPLHPPSHKG AAWSGTDPKP RGLSLDAGDS
110 120 130 140 150
DLQVREDIRN RTLRAVCGQP GMPRDPWDLP VGQRRTLLRH ILVSDRYRFL
160 170 180 190 200
YCYVPKVACS NWKRVLKVLA GILNNVDVRL KMDHRSDLVF LADLRPEEIR
210 220 230 240 250
YRLQHYFKFL FVRDPLERLL SAYRNKFGEI REYQQRYGAE IVRRYRAGAG
260 270 280 290 300
PSPAGDDVTF PEFLRYLVDE DPEHMNEHWM PVYHLCQPCA VHYDFVGSYE
310 320 330 340 350
RLEADANQVL EWVRAPPHVR FPARQAWYRP ASPESLHYHL CNVPRALLQD
360 370
VLPKYILDFS LFAYPLPNVT KEACHQ
Length:376
Mass (Da):43,145
Last modified:March 15, 2005 - v2
Checksum:i319E5A82E3AA4ACF
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2AQV2A2AQV2_MOUSE
Carbohydrate sulfotransferase
Chst14
351Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti172I → V in AAH43700 (PubMed:15489334).Curated1
Sequence conflicti172I → V in AAH26886 (PubMed:15489334).Curated1
Sequence conflicti185R → P in AAH43700 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011230 mRNA Translation: BAB27480.1
AK154636 mRNA Translation: BAE32731.1
AK159357 mRNA Translation: BAE35015.1
AK171614 mRNA Translation: BAE42565.1
AL845164 Genomic DNA No translation available.
BC026886 mRNA Translation: AAH26886.1
BC043700 mRNA Translation: AAH43700.1
BC085479 mRNA Translation: AAH85479.1
CCDSiCCDS16587.1
RefSeqiNP_082393.3, NM_028117.3
UniGeneiMm.486238
Mm.490292

Genome annotation databases

EnsembliENSMUST00000099546; ENSMUSP00000099579; ENSMUSG00000074916
GeneIDi72136
KEGGimmu:72136
UCSCiuc012cbn.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011230 mRNA Translation: BAB27480.1
AK154636 mRNA Translation: BAE32731.1
AK159357 mRNA Translation: BAE35015.1
AK171614 mRNA Translation: BAE42565.1
AL845164 Genomic DNA No translation available.
BC026886 mRNA Translation: AAH26886.1
BC043700 mRNA Translation: AAH43700.1
BC085479 mRNA Translation: AAH85479.1
CCDSiCCDS16587.1
RefSeqiNP_082393.3, NM_028117.3
UniGeneiMm.486238
Mm.490292

3D structure databases

ProteinModelPortaliQ80V53
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80V53, 2 interactors
MINTiQ80V53
STRINGi10090.ENSMUSP00000099579

PTM databases

PhosphoSitePlusiQ80V53

Proteomic databases

MaxQBiQ80V53
PaxDbiQ80V53
PeptideAtlasiQ80V53
PRIDEiQ80V53

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099546; ENSMUSP00000099579; ENSMUSG00000074916
GeneIDi72136
KEGGimmu:72136
UCSCiuc012cbn.1 mouse

Organism-specific databases

CTDi113189
MGIiMGI:1919386 Chst14

Phylogenomic databases

eggNOGiKOG4651 Eukaryota
ENOG4111GJR LUCA
GeneTreeiENSGT00760000119214
HOGENOMiHOG000231801
HOVERGENiHBG050954
InParanoidiQ80V53
KOiK08105
OMAiRYRLQHY
OrthoDBiEOG091G0FZO
PhylomeDBiQ80V53
TreeFamiTF325581

Enzyme and pathway databases

BRENDAi2.8.2.35 3474
ReactomeiR-MMU-2022923 Dermatan sulfate biosynthesis

Miscellaneous databases

PROiPR:Q80V53
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000074916 Expressed in 209 organ(s), highest expression level in humerus cartilage element
ExpressionAtlasiQ80V53 baseline and differential
GenevisibleiQ80V53 MM

Family and domain databases

InterProiView protein in InterPro
IPR018011 Carb_sulfotrans_8-10
IPR005331 Sulfotransferase
PANTHERiPTHR12137 PTHR12137, 1 hit
PfamiView protein in Pfam
PF03567 Sulfotransfer_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCHSTE_MOUSE
AccessioniPrimary (citable) accession number: Q80V53
Secondary accession number(s): A2AQV3
, Q3TXA1, Q8R304, Q9D0P2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: September 12, 2018
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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