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Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC1

Gene

Polr2e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-113418 Formation of the Early Elongation Complex
R-MMU-5250924 B-WICH complex positively regulates rRNA expression
R-MMU-5578749 Transcriptional regulation by small RNAs
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6781823 Formation of TC-NER Pre-Incision Complex
R-MMU-6782135 Dual incision in TC-NER
R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-MMU-6803529 FGFR2 alternative splicing
R-MMU-6807505 RNA polymerase II transcribes snRNA genes
R-MMU-72086 mRNA Capping
R-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72165 mRNA Splicing - Minor Pathway
R-MMU-72203 Processing of Capped Intron-Containing Pre-mRNA
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-73772 RNA Polymerase I Promoter Escape
R-MMU-73776 RNA Polymerase II Promoter Escape
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-MMU-73863 RNA Polymerase I Transcription Termination
R-MMU-75953 RNA Polymerase II Transcription Initiation
R-MMU-75955 RNA Polymerase II Transcription Elongation
R-MMU-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-MMU-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter
R-MMU-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter
R-MMU-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter
R-MMU-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-MMU-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I, II, and III subunit RPABC1
Short name:
RNA polymerases I, II, and III subunit ABC1
Alternative name(s):
DNA-directed RNA polymerase II subunit E
RPB5 homolog
Gene namesi
Name:Polr2e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1913670 Polr2e

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001460761 – 210DNA-directed RNA polymerases I, II, and III subunit RPABC1Add BLAST210

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Cross-linki81Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ80UW8
MaxQBiQ80UW8
PaxDbiQ80UW8
PRIDEiQ80UW8

PTM databases

iPTMnetiQ80UW8
PhosphoSitePlusiQ80UW8

Expressioni

Gene expression databases

BgeeiENSMUSG00000004667 Expressed in 293 organ(s), highest expression level in embryo
CleanExiMM_POLR2E
ExpressionAtlasiQ80UW8 baseline and differential
GenevisibleiQ80UW8 MM

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively. In RNA Pol II, this subunit is present in 2-fold molar excess over the other subunits. Interacts with URI1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi211461, 2 interactors
IntActiQ80UW8, 3 interactors
STRINGi10090.ENSMUSP00000004786

Structurei

3D structure databases

ProteinModelPortaliQ80UW8
SMRiQ80UW8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3218 Eukaryota
COG2012 LUCA
GeneTreeiENSGT00390000013841
HOGENOMiHOG000205213
HOVERGENiHBG057019
InParanoidiQ80UW8
KOiK03013
OMAiNQLMRIQ
OrthoDBiEOG091G0JI0
PhylomeDBiQ80UW8
TreeFamiTF103040

Family and domain databases

Gene3Di3.40.1340.10, 1 hit
3.90.940.20, 1 hit
HAMAPiMF_00025 RNApol_RpoH_RPB5, 1 hit
InterProiView protein in InterPro
IPR014381 DNA_RNA_pol_RPB5_euk/virus
IPR005571 RNA_pol_Rpb5_N
IPR036710 RNA_pol_Rpb5_N_sf
IPR000783 RNA_pol_subH/Rpb5_C
IPR020608 RNA_pol_subH/Rpb5_CS
IPR039531 Rpb5-like
IPR035913 RPB5-like_sf
IPR020609 RpoH/RPB5
PANTHERiPTHR10535 PTHR10535, 1 hit
PfamiView protein in Pfam
PF01191 RNA_pol_Rpb5_C, 1 hit
PF03871 RNA_pol_Rpb5_N, 1 hit
PIRSFiPIRSF000747 RPB5, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD005155 RNA_pol_subH/Rpb5_C, 1 hit
SUPFAMiSSF53036 SSF53036, 1 hit
SSF55287 SSF55287, 1 hit
PROSITEiView protein in PROSITE
PS01110 RNA_POL_H_23KD, 1 hit

Sequencei

Sequence statusi: Complete.

Q80UW8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQFGDKPSE
60 70 80 90 100
GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKVY CQRMQEENIT
110 120 130 140 150
RALIVVQQGM TPSAKQSLVD MAPKYVLEQF LQQELLINIT EHELVPEHVV
160 170 180 190 200
MTKEEVTELL ARYKLRESQL PRIQAGDPVA RYFGIKRGQV VKIIRPSETA
210
GRYITYRLVQ
Length:210
Mass (Da):24,570
Last modified:June 1, 2003 - v1
Checksum:iE09DEB40E05F3554
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC045521 mRNA Translation: AAH45521.1
CCDSiCCDS35972.1
RefSeqiNP_079830.2, NM_025554.2
UniGeneiMm.18579

Genome annotation databases

EnsembliENSMUST00000004786; ENSMUSP00000004786; ENSMUSG00000004667
GeneIDi66420
KEGGimmu:66420
UCSCiuc007gbi.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC045521 mRNA Translation: AAH45521.1
CCDSiCCDS35972.1
RefSeqiNP_079830.2, NM_025554.2
UniGeneiMm.18579

3D structure databases

ProteinModelPortaliQ80UW8
SMRiQ80UW8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211461, 2 interactors
IntActiQ80UW8, 3 interactors
STRINGi10090.ENSMUSP00000004786

PTM databases

iPTMnetiQ80UW8
PhosphoSitePlusiQ80UW8

Proteomic databases

EPDiQ80UW8
MaxQBiQ80UW8
PaxDbiQ80UW8
PRIDEiQ80UW8

Protocols and materials databases

DNASUi66420
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004786; ENSMUSP00000004786; ENSMUSG00000004667
GeneIDi66420
KEGGimmu:66420
UCSCiuc007gbi.1 mouse

Organism-specific databases

CTDi5434
MGIiMGI:1913670 Polr2e

Phylogenomic databases

eggNOGiKOG3218 Eukaryota
COG2012 LUCA
GeneTreeiENSGT00390000013841
HOGENOMiHOG000205213
HOVERGENiHBG057019
InParanoidiQ80UW8
KOiK03013
OMAiNQLMRIQ
OrthoDBiEOG091G0JI0
PhylomeDBiQ80UW8
TreeFamiTF103040

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-113418 Formation of the Early Elongation Complex
R-MMU-5250924 B-WICH complex positively regulates rRNA expression
R-MMU-5578749 Transcriptional regulation by small RNAs
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6781823 Formation of TC-NER Pre-Incision Complex
R-MMU-6782135 Dual incision in TC-NER
R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-MMU-6803529 FGFR2 alternative splicing
R-MMU-6807505 RNA polymerase II transcribes snRNA genes
R-MMU-72086 mRNA Capping
R-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72165 mRNA Splicing - Minor Pathway
R-MMU-72203 Processing of Capped Intron-Containing Pre-mRNA
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-73772 RNA Polymerase I Promoter Escape
R-MMU-73776 RNA Polymerase II Promoter Escape
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-MMU-73863 RNA Polymerase I Transcription Termination
R-MMU-75953 RNA Polymerase II Transcription Initiation
R-MMU-75955 RNA Polymerase II Transcription Elongation
R-MMU-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-MMU-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter
R-MMU-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter
R-MMU-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter
R-MMU-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-MMU-9018519 Estrogen-dependent gene expression

Miscellaneous databases

PROiPR:Q80UW8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004667 Expressed in 293 organ(s), highest expression level in embryo
CleanExiMM_POLR2E
ExpressionAtlasiQ80UW8 baseline and differential
GenevisibleiQ80UW8 MM

Family and domain databases

Gene3Di3.40.1340.10, 1 hit
3.90.940.20, 1 hit
HAMAPiMF_00025 RNApol_RpoH_RPB5, 1 hit
InterProiView protein in InterPro
IPR014381 DNA_RNA_pol_RPB5_euk/virus
IPR005571 RNA_pol_Rpb5_N
IPR036710 RNA_pol_Rpb5_N_sf
IPR000783 RNA_pol_subH/Rpb5_C
IPR020608 RNA_pol_subH/Rpb5_CS
IPR039531 Rpb5-like
IPR035913 RPB5-like_sf
IPR020609 RpoH/RPB5
PANTHERiPTHR10535 PTHR10535, 1 hit
PfamiView protein in Pfam
PF01191 RNA_pol_Rpb5_C, 1 hit
PF03871 RNA_pol_Rpb5_N, 1 hit
PIRSFiPIRSF000747 RPB5, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD005155 RNA_pol_subH/Rpb5_C, 1 hit
SUPFAMiSSF53036 SSF53036, 1 hit
SSF55287 SSF55287, 1 hit
PROSITEiView protein in PROSITE
PS01110 RNA_POL_H_23KD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPAB1_MOUSE
AccessioniPrimary (citable) accession number: Q80UW8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: June 1, 2003
Last modified: November 7, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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