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Protein

Serine/threonine-protein kinase MRCK gamma

Gene

Cdc42bpg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Enzyme regulationi

Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100ATPPROSITE-ProRule annotationBy similarity1
Active sitei195Proton acceptorPROSITE-ProRule annotationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 85ATPPROSITE-ProRule annotationBy similarity9
Zinc fingeri877 – 926Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase MRCK gamma (EC:2.7.11.1)
Alternative name(s):
CDC42-binding protein kinase gamma
DMPK-like gamma
Myotonic dystrophy kinase-related CDC42-binding kinase gamma
Short name:
MRCK gamma
Short name:
Myotonic dystrophy protein kinase-like gamma
Myotonic dystrophy protein kinase-like alpha
Gene namesi
Name:Cdc42bpgImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2652845 Cdc42bpg

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000863981 – 1551Serine/threonine-protein kinase MRCK gammaAdd BLAST1551

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei216Phosphoserine; by autocatalysisBy similarity1
Modified residuei228Phosphoserine; by autocatalysisBy similarity1
Modified residuei234Phosphothreonine; by autocatalysisBy similarity1
Modified residuei1481PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ80UW5
PaxDbiQ80UW5
PeptideAtlasiQ80UW5
PRIDEiQ80UW5

PTM databases

iPTMnetiQ80UW5
PhosphoSitePlusiQ80UW5

Expressioni

Gene expression databases

BgeeiENSMUSG00000024769
CleanExiMM_CDC42BPG
GenevisibleiQ80UW5 MM

Interactioni

Subunit structurei

Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42 (By similarity).By similarity

Protein-protein interaction databases

BioGridi232205, 1 interactor
IntActiQ80UW5, 1 interactor
STRINGi10090.ENSMUSP00000025681

Structurei

3D structure databases

ProteinModelPortaliQ80UW5
SMRiQ80UW5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini71 – 337Protein kinasePROSITE-ProRule annotationBy similarityAdd BLAST267
Domaini338 – 408AGC-kinase C-terminalAdd BLAST71
Domaini946 – 1065PHPROSITE-ProRule annotationAdd BLAST120
Domaini1091 – 1365CNHPROSITE-ProRule annotationAdd BLAST275
Domaini1436 – 1449CRIBPROSITE-ProRule annotationAdd BLAST14

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili442 – 675Sequence analysisAdd BLAST234
Coiled coili729 – 801Sequence analysisAdd BLAST73

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1289 – 1292Poly-LeuSequence analysis4

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri877 – 926Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0612 Eukaryota
ENOG410XR1Q LUCA
GeneTreeiENSGT00760000118994
HOGENOMiHOG000294133
HOVERGENiHBG055933
InParanoidiQ80UW5
KOiK16307
OMAiMKRKPWT
OrthoDBiEOG091G09EX
PhylomeDBiQ80UW5
TreeFamiTF313551

Family and domain databases

CDDicd00029 C1, 1 hit
Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR001180 CNH_dom
IPR000095 CRIB_dom
IPR011009 Kinase-like_dom_sf
IPR033235 MRCK_gamma
IPR014930 Myotonic_dystrophy_kinase_coil
IPR002219 PE/DAG-bd
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR017892 Pkinase_C
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR22988:SF22 PTHR22988:SF22, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF00780 CNH, 1 hit
PF08826 DMPK_coil, 1 hit
PF00069 Pkinase, 1 hit
PF00433 Pkinase_C, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD011252 Myotonic_dystrophy_kinase_coil, 1 hit
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00036 CNH, 1 hit
SM00285 PBD, 1 hit
SM00233 PH, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS50219 CNH, 1 hit
PS50108 CRIB, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q80UW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQRLRALEQ LVRGEAGGSP GLDGLLDLLL GVHQELSSAP LRRERNVAQF
60 70 80 90 100
LSWASPFVTK VKELRLQRDD FEILKVIGRG AFGEVAVVRQ RGSGQIFAMK
110 120 130 140 150
MLHKWEMLKR AETACFREER DVLVKGDSRW VTALHYAFQD EEYLYLVMDY
160 170 180 190 200
YAGGDLLTLL SRFEDRLPPE LAQFYLAEMV LAIHSLHQLG YVHRDVKPDN
210 220 230 240 250
ILLDMNGHIR LADFGSCLRL NNNGMVDSSV AVGTPDYISP EILQAMEEGK
260 270 280 290 300
GHYGPQCDWW SLGVCAYELL FGETPFYAES LVETYGKIMN HEDHLQFPAD
310 320 330 340 350
VTDVPASAQD LIRQLLCRQE ERLGRGGLDD FRKHPFFEGV DWERLATSTA
360 370 380 390 400
PYIPELRGPM DTSNFDVDDD TLNRPETLPP SSHGAFSGHH LPFVGFTYTS
410 420 430 440 450
GSPFDVQSSE LMAAPEGTPH CVEQVKVELS HKCQEPLHGP LQPQELVRLQ
460 470 480 490 500
KEVQVLQEKL AETLRDSKAS LSQTDGLHAR SPAPNIQLQQ EKDRLQQELT
510 520 530 540 550
EAQAALRVQD AELCQAQNRQ EEFLQRLWEA QEREAAAASQ IQALNSQLEE
560 570 580 590 600
AWVVRRELEG QVTTLSQEVT RLQGQCKQES SQAKTVHAAP ETNGIGSPEG
610 620 630 640 650
QSQEAQLRKE VAALREQLEH ACSQGISVGK EEVLCRLQEE NQRLSREQER
660 670 680 690 700
LAGELELELQ SKQRLEGERR ETESNWEAQI ADILSWVNDE KVSRGYLQAL
710 720 730 740 750
ATKMAEELES LRNVGTQTLP TRPLDHQWKA RRLQKMEASA RLELQSALEA
760 770 780 790 800
EIRAKQSLQE QLTQVQEAQR QAERRLQEAE KQSQALQQEV AELREELQAR
810 820 830 840 850
GPGDARPSTS LIPLLSFWNT EKDSAKDPGN SGEGPRSGAE AELRPEGRRS
860 870 880 890 900
LRMGSVFPRV PAATTTPAEG PPAKPGSHTL RPRSFPSPTK CLRCTSLMLG
910 920 930 940 950
LGRQGLGCDT CGYFCHSACA SQAPPCPVPP ELLRTALGVH PETGTGTAYE
960 970 980 990 1000
GFLSVPRPSG VRRGWQRVYA ALSDSRLLLF DAPDPRGSLA SGVLLQALDL
1010 1020 1030 1040 1050
RDPQFSATPV LAPDVIHAQS KDLPRIFRVT ASQLTVPPTT CTVLLLAENE
1060 1070 1080 1090 1100
GERERWLQVL GELQRLLLDA RPRPRPVYTL KEAYDNGLPL LPHALCAAVI
1110 1120 1130 1140 1150
DQERLALGTE EGLFVIHLHS NDIFQVGDCR RVQRLAVSSA AGLLAVLCGR
1160 1170 1180 1190 1200
GPSVRLFALD ELESAEVAGA KIPESRGCQA LVAGRILQAR TPVLCVAVKR
1210 1220 1230 1240 1250
QVLCYQLGPG PGPWQRRIRE LQAPAPVQSL GLLGDRLCVG AAGTFALYPL
1260 1270 1280 1290 1300
LNEAAPLALG TGLVAEELPA SRGGLGEALG AVELSLSELL LLFATAGVYV
1310 1320 1330 1340 1350
DSAGRKSRSH ELLWPAAPTG WGYTAPYLTV FSENALDVFD VRRAEWVQTV
1360 1370 1380 1390 1400
PLKKVRPLNP EGSLFLYGTE KVRLTYLRNP LAEKDEFDIP DLTDNSRRQL
1410 1420 1430 1440 1450
FRTKSKRRFF FRVSDELRQQ QRREMLKDPF VRSKFISPPT NFNHLVHVGP
1460 1470 1480 1490 1500
TEGRPNTRDG TRAQEQKSRG ARSSGPQRPH SFSEAFRRPV STGSDGLPGE
1510 1520 1530 1540 1550
TDPLVKRKPW TSLSSESVSC PQGSLSPAAS LIQVSERPRS LPPDPESESS

P
Length:1,551
Mass (Da):172,147
Last modified:November 8, 2005 - v2
Checksum:i74458CA5FEF6F8A7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1065R → Q in AAH46418 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC127556 Genomic DNA No translation available.
BC046418 mRNA Translation: AAH46418.1
CCDSiCCDS50365.1
RefSeqiNP_001028514.1, NM_001033342.1
UniGeneiMm.329622

Genome annotation databases

EnsembliENSMUST00000025681; ENSMUSP00000025681; ENSMUSG00000024769
ENSMUST00000201699; ENSMUSP00000144638; ENSMUSG00000107268
GeneIDi240505
KEGGimmu:240505
UCSCiuc008gia.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMRCKG_MOUSE
AccessioniPrimary (citable) accession number: Q80UW5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: March 28, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

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