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Protein

F-box only protein 2

Gene

Fbxo2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Involved in the endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranslocated into the cytosol and promoting their ubiquitination and subsequent degradation. Prevents formation of cytosolic aggregates of unfolded glycoproteins that have been retrotranslocated into the cytosol. Able to recognize and bind denatured glycoproteins, preferentially those of the high-mannose type.5 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei159Important for carbohydrate binding1
Sitei177Important for carbohydrate binding1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway
LigandLectin

Enzyme and pathway databases

ReactomeiR-MMU-8951664 Neddylation
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 2
Gene namesi
Name:Fbxo2
Synonyms:Fbs1, Fbx2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2446216 Fbxo2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Disruption phenotypei

No visible phenotype at birth. Mice are viable and fertile, but after two to four months, gradual hearing loss sets in, due to degeneration of epithelial support cells and hair cells of the organ of Corti and spiral ganglion neurodegeneration.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi177F → A: Abolishes binding of glycoprotein targets. 1 Publication1
Mutagenesisi279Y → A: Abolishes binding of glycoprotein targets. 1 Publication1
Mutagenesisi280W → A: Abolishes binding of glycoprotein targets. Strongly reduced ubiquitination of glycoprotein targets. 1 Publication1
Mutagenesisi281K → A: No effect on carbohydrate binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198761 – 297F-box only protein 2Add BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei106PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ80UW2
MaxQBiQ80UW2
PaxDbiQ80UW2
PRIDEiQ80UW2

PTM databases

iPTMnetiQ80UW2
PhosphoSitePlusiQ80UW2
SwissPalmiQ80UW2

Expressioni

Tissue specificityi

Detected in brain and cochlea, in epithelial support cells and hair cells of the organ of Corti (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000041556 Expressed in 115 organ(s), highest expression level in vestibular membrane of cochlear duct
CleanExiMM_FBXO2
ExpressionAtlasiQ80UW2 baseline and differential
GenevisibleiQ80UW2 MM

Interactioni

Subunit structurei

Component of the SCF(FBXO2) complex consisting of CUL1, RBX1, SKP1 and FBXO2. Predominantly detected as heterodimer with SKP1; the heterodimer with SKP1 is not part of the SCF(FBXO2) complex.3 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi231053, 9 interactors
CORUMiQ80UW2
DIPiDIP-54758N
IntActiQ80UW2, 5 interactors
STRINGi10090.ENSMUSP00000037377

Structurei

Secondary structure

1297
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ80UW2
SMRiQ80UW2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ80UW2

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 95F-boxPROSITE-ProRule annotationAdd BLAST48
Domaini117 – 297FBAPROSITE-ProRule annotationAdd BLAST181

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni214 – 216Carbohydrate binding3
Regioni279 – 280Carbohydrate binding2

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi14 – 56Glu-richAdd BLAST43

Phylogenomic databases

eggNOGiENOG410IK6V Eukaryota
ENOG4111MF5 LUCA
GeneTreeiENSGT00390000003865
HOGENOMiHOG000231084
HOVERGENiHBG003593
InParanoidiQ80UW2
KOiK10099
OMAiIHDESVK
OrthoDBiEOG091G0G3L
PhylomeDBiQ80UW2
TreeFamiTF320527

Family and domain databases

Gene3Di2.60.120.260, 1 hit
InterProiView protein in InterPro
IPR007397 F-box-assoc_dom
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
IPR039752 F-box_only
IPR008979 Galactose-bd-like_sf
PANTHERiPTHR12125 PTHR12125, 1 hit
PfamiView protein in Pfam
PF00646 F-box, 1 hit
PF04300 FBA, 1 hit
SMARTiView protein in SMART
SM01198 FBA, 1 hit
SUPFAMiSSF49785 SSF49785, 1 hit
SSF81383 SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS51114 FBA, 1 hit
PS50181 FBOX, 1 hit

Sequencei

Sequence statusi: Complete.

Q80UW2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDGDGDPESV SHPEEASPEE QPEEAGAEAS AEEEQLREAE EEEEAEAVEY
60 70 80 90 100
LAELPEPLLL RVLAELPATE LVQACRLVCL RWKELVDGAP LWLLKCQQEG
110 120 130 140 150
LVPEGSADEE RDHWQQFYFL SKRRRNLLRN PCGEEDLEGW SDVEHGGDGW
160 170 180 190 200
RVEELPGDNG VEFTQDDSVK KYFASSFEWC RKAQVIDLQA EGYWEELLDT
210 220 230 240 250
TQPAIVVKDW YSGRTDAGSL YELTVRLLSE NEDVLAEFAT GQVAVPEDGS
260 270 280 290
WMEISHTFID YGPGVRFVRF EHGGQDSVYW KGWFGARVTN SSVWVEP
Length:297
Mass (Da):33,676
Last modified:June 1, 2003 - v1
Checksum:iE0F8A5601CE5930C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC027053 mRNA Translation: AAH27053.1
BC046586 mRNA Translation: AAH46586.1
CCDSiCCDS18935.1
RefSeqiNP_789818.1, NM_176848.1
XP_006538877.1, XM_006538814.3
UniGeneiMm.262287

Genome annotation databases

EnsembliENSMUST00000047951; ENSMUSP00000037377; ENSMUSG00000041556
GeneIDi230904
KEGGimmu:230904
UCSCiuc008vuk.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC027053 mRNA Translation: AAH27053.1
BC046586 mRNA Translation: AAH46586.1
CCDSiCCDS18935.1
RefSeqiNP_789818.1, NM_176848.1
XP_006538877.1, XM_006538814.3
UniGeneiMm.262287

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UMHX-ray2.00A117-297[»]
1UMIX-ray2.40A117-297[»]
2E31X-ray2.40A1-297[»]
2E32X-ray3.52A/C1-297[»]
2E33X-ray2.70A105-297[»]
2RJ2X-ray1.70A117-297[»]
5B4NX-ray2.30A/B117-297[»]
ProteinModelPortaliQ80UW2
SMRiQ80UW2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231053, 9 interactors
CORUMiQ80UW2
DIPiDIP-54758N
IntActiQ80UW2, 5 interactors
STRINGi10090.ENSMUSP00000037377

PTM databases

iPTMnetiQ80UW2
PhosphoSitePlusiQ80UW2
SwissPalmiQ80UW2

Proteomic databases

EPDiQ80UW2
MaxQBiQ80UW2
PaxDbiQ80UW2
PRIDEiQ80UW2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047951; ENSMUSP00000037377; ENSMUSG00000041556
GeneIDi230904
KEGGimmu:230904
UCSCiuc008vuk.1 mouse

Organism-specific databases

CTDi26232
MGIiMGI:2446216 Fbxo2

Phylogenomic databases

eggNOGiENOG410IK6V Eukaryota
ENOG4111MF5 LUCA
GeneTreeiENSGT00390000003865
HOGENOMiHOG000231084
HOVERGENiHBG003593
InParanoidiQ80UW2
KOiK10099
OMAiIHDESVK
OrthoDBiEOG091G0G3L
PhylomeDBiQ80UW2
TreeFamiTF320527

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-8951664 Neddylation
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiFbxo2 mouse
EvolutionaryTraceiQ80UW2
PROiPR:Q80UW2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041556 Expressed in 115 organ(s), highest expression level in vestibular membrane of cochlear duct
CleanExiMM_FBXO2
ExpressionAtlasiQ80UW2 baseline and differential
GenevisibleiQ80UW2 MM

Family and domain databases

Gene3Di2.60.120.260, 1 hit
InterProiView protein in InterPro
IPR007397 F-box-assoc_dom
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
IPR039752 F-box_only
IPR008979 Galactose-bd-like_sf
PANTHERiPTHR12125 PTHR12125, 1 hit
PfamiView protein in Pfam
PF00646 F-box, 1 hit
PF04300 FBA, 1 hit
SMARTiView protein in SMART
SM01198 FBA, 1 hit
SUPFAMiSSF49785 SSF49785, 1 hit
SSF81383 SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS51114 FBA, 1 hit
PS50181 FBOX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFBX2_MOUSE
AccessioniPrimary (citable) accession number: Q80UW2
Secondary accession number(s): Q8R0D2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: June 1, 2003
Last modified: November 7, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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