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UniProtKB - Q80US8 (MAD3_MOUSE)
Protein
Max dimerization protein 3
Gene
Mxd3
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.
1 PublicationMiscellaneous
Mice deficient for Mxd3 show increased sensitivity of neuronal and lymphoid cells to gamma-radiation induced apoptosis.
GO - Molecular functioni
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Max dimerization protein 3Short name: Max dimerizer 3 Alternative name(s): Max-associated protein 3 |
Gene namesi | Name:Mxd3 Synonyms:Mad3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104987, Mxd3 |
VEuPathDBi | HostDB:ENSMUSG00000021485 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation1 Publication
Nucleus
- RNA polymerase II transcription regulator complex Source: NTNU_SB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000253708 | 1 – 206 | Max dimerization protein 3Add BLAST | 206 |
Proteomic databases
EPDi | Q80US8 |
PaxDbi | Q80US8 |
PRIDEi | Q80US8 |
ProteomicsDBi | 291999 |
PTM databases
PhosphoSitePlusi | Q80US8 |
Expressioni
Tissue specificityi
Expressed only in the proliferating areas of the testis and thymus.1 Publication
Developmental stagei
Expressed during neural and epidermal differentiation. Expression restricted to proliferating cells prior to differentiation. Specifically expressed in the S phase of the cell cycle in neuronal progenitor cells. In the developing embryo, detected from 9.5 to 12.5 dpc especially in the ventricular zone of the neuroepithelia, in the progression zone of the limb buds and in the aortic arches and liver. In the spinal cord at embryonic days 10.5, 11.5 and 12.5 dpc, expressed in the cells at the perimeter of the ventricular zone. In the developing epidermis, expressed only in the uppermost differentiated cell layers underneath the stratum corneum.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000021485, Expressed in bone marrow and 264 other tissues |
Genevisiblei | Q80US8, MM |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX.
Interacts with SIN3A AND SIN3B.
Interacts with RNF17.
2 PublicationsGO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 201271, 1 interactor |
DIPi | DIP-948N |
STRINGi | 10090.ENSMUSP00000021941 |
Miscellaneous databases
RNActi | Q80US8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 57 – 109 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 8 – 25 | Interaction with SIN3A and SIN3B1 PublicationAdd BLAST | 18 | |
Regioni | 29 – 66 | DisorderedSequence analysisAdd BLAST | 38 | |
Regioni | 122 – 171 | DisorderedSequence analysisAdd BLAST | 50 |
Phylogenomic databases
eggNOGi | KOG2483, Eukaryota |
GeneTreei | ENSGT00940000161050 |
HOGENOMi | CLU_082604_1_0_1 |
InParanoidi | Q80US8 |
OMAi | IVFDCVD |
OrthoDBi | 1545091at2759 |
PhylomeDBi | Q80US8 |
TreeFami | TF315654 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
i Sequence
Sequence statusi: Complete.
Q80US8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEPVASNIQV LLQAAEFLER REREAEHGYA SLCPHHSPGT VCRRRKPPLQ
60 70 80 90 100
APGALNSGRS VHNELEKRRR AQLKRCLEQL RQQMPLGVDC TRYTTLSLLR
110 120 130 140 150
RARVHIQKLE EQEQQARRLK EKLRSKQQSL QQQLEQLQGL PGARERERLR
160 170 180 190 200
ADSLDSSGLS SERSDSDQED LEVDVESLVF GTETELLQSF SAGREHSYSH
STCAWL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 177 | S → N in AAB02794 (PubMed:8521822).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U32394 mRNA Translation: AAB02794.1 AK138742 mRNA Translation: BAE23764.1 BC051970 mRNA Translation: AAH51970.1 |
CCDSi | CCDS26543.1 |
PIRi | S60005 |
RefSeqi | NP_057871.2, NM_016662.4 |
Genome annotation databases
Ensembli | ENSMUST00000021941; ENSMUSP00000021941; ENSMUSG00000021485 |
GeneIDi | 17121 |
KEGGi | mmu:17121 |
UCSCi | uc007qqn.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U32394 mRNA Translation: AAB02794.1 AK138742 mRNA Translation: BAE23764.1 BC051970 mRNA Translation: AAH51970.1 |
CCDSi | CCDS26543.1 |
PIRi | S60005 |
RefSeqi | NP_057871.2, NM_016662.4 |
3D structure databases
AlphaFoldDBi | Q80US8 |
SMRi | Q80US8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201271, 1 interactor |
DIPi | DIP-948N |
STRINGi | 10090.ENSMUSP00000021941 |
PTM databases
PhosphoSitePlusi | Q80US8 |
Proteomic databases
EPDi | Q80US8 |
PaxDbi | Q80US8 |
PRIDEi | Q80US8 |
ProteomicsDBi | 291999 |
Protocols and materials databases
ABCDi | Q80US8, 1 sequenced antibody |
Antibodypediai | 17372, 235 antibodies from 29 providers |
DNASUi | 17121 |
Genome annotation databases
Ensembli | ENSMUST00000021941; ENSMUSP00000021941; ENSMUSG00000021485 |
GeneIDi | 17121 |
KEGGi | mmu:17121 |
UCSCi | uc007qqn.1, mouse |
Organism-specific databases
CTDi | 83463 |
MGIi | MGI:104987, Mxd3 |
VEuPathDBi | HostDB:ENSMUSG00000021485 |
Phylogenomic databases
eggNOGi | KOG2483, Eukaryota |
GeneTreei | ENSGT00940000161050 |
HOGENOMi | CLU_082604_1_0_1 |
InParanoidi | Q80US8 |
OMAi | IVFDCVD |
OrthoDBi | 1545091at2759 |
PhylomeDBi | Q80US8 |
TreeFami | TF315654 |
Miscellaneous databases
BioGRID-ORCSi | 17121, 3 hits in 76 CRISPR screens |
PROi | PR:Q80US8 |
RNActi | Q80US8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021485, Expressed in bone marrow and 264 other tissues |
Genevisiblei | Q80US8, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MAD3_MOUSE | |
Accessioni | Q80US8Primary (citable) accession number: Q80US8 Secondary accession number(s): Q60947 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 17, 2006 |
Last sequence update: | June 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot