UniProtKB - Q80UF7 (TCAM1_MOUSE)
TIR domain-containing adapter molecule 1
Ticam1
Functioni
GO - Molecular functioni
- protein kinase binding Source: MGI
GO - Biological processi
- apoptotic signaling pathway Source: MGI
- cellular response to lipopolysaccharide Source: ParkinsonsUK-UCL
- cellular response to oxidised low-density lipoprotein particle stimulus Source: ARUK-UCL
- defense response to virus Source: MGI
- inflammatory response Source: UniProtKB-KW
- innate immune response Source: UniProtKB-KW
- lipopolysaccharide-mediated signaling pathway Source: MGI
- macrophage activation involved in immune response Source: MGI
- MyD88-independent toll-like receptor signaling pathway Source: MGI
- positive regulation of autophagy Source: ParkinsonsUK-UCL
- positive regulation of B cell activation Source: MGI
- positive regulation of B cell proliferation Source: MGI
- positive regulation of chemokine production Source: MGI
- positive regulation of cytokine production involved in inflammatory response Source: ARUK-UCL
- positive regulation of gene expression Source: ARUK-UCL
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- positive regulation of interferon-beta production Source: MGI
- positive regulation of interleukin-6 production Source: MGI
- positive regulation of myeloid dendritic cell cytokine production Source: UniProtKB
- positive regulation of natural killer cell activation Source: MGI
- positive regulation of NF-kappaB transcription factor activity Source: MGI
- positive regulation of nitric oxide biosynthetic process Source: MGI
- positive regulation of protein binding Source: MGI
- positive regulation of protein ubiquitination Source: MGI
- positive regulation of tumor necrosis factor production Source: MGI
- positive regulation of type I interferon production Source: MGI
- regulation of protein-containing complex assembly Source: MGI
- response to exogenous dsRNA Source: MGI
- response to lipopolysaccharide Source: MGI
- TRIF-dependent toll-like receptor signaling pathway Source: ParkinsonsUK-UCL
Keywordsi
Biological process | Antiviral defense, Apoptosis, Immunity, Inflammatory response, Innate immunity |
Enzyme and pathway databases
Reactomei | R-MMU-140534, Caspase activation via Death Receptors in the presence of ligand R-MMU-166166, MyD88-independent TLR4 cascade R-MMU-2562578, TRIF-mediated programmed cell death R-MMU-936964, Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon R-MMU-937041, IKK complex recruitment mediated by RIP1 R-MMU-937072, TRAF6-mediated induction of TAK1 complex within TLR4 complex R-MMU-975163, IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation |
Names & Taxonomyi
Protein namesi | Recommended name: TIR domain-containing adapter molecule 1Short name: TICAM-1 Alternative name(s): Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta Short name: TIR domain-containing adapter protein inducing IFN-beta |
Gene namesi | Name:Ticam1 Synonyms:Trif |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2147032, Ticam1 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Cytoplasm and Cytosol
- cytosol 1 Publication
Other locations
- autophagosome By similarity
Note: Colocalizes with UBQLN1 in the autophagosome. Colocalizes in the cytosol with DDX1, DDX21 and DHX36 (PubMed:21703541). Colocalizes in the mitochondria with DDX1 and poly(I:C) RNA ligand (PubMed:21703541). The multi-helicase-TICAM1 complex may translocate to the mitochondria upon poly(I:C) RNA ligand stimulation (PubMed:21703541).By similarity1 Publication
Cytosol
- cytosol Source: UniProtKB
- ripoptosome Source: MGI
Mitochondrion
- mitochondrion Source: UniProtKB
Other locations
- autophagosome Source: UniProtKB-SubCell
- cytoplasmic vesicle Source: UniProtKB-KW
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, MitochondrionPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000317664 | 1 – 732 | TIR domain-containing adapter molecule 1Add BLAST | 732 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 209 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q80UF7 |
PaxDbi | Q80UF7 |
PRIDEi | Q80UF7 |
ProteomicsDBi | 263143 |
PTM databases
PhosphoSitePlusi | Q80UF7 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000047123, Expressed in intestine and 176 other tissues |
Genevisiblei | Q80UF7, MM |
Interactioni
Subunit structurei
Homodimer (By similarity).
Found in a multi-helicase-TICAM1 complex at least composed of DHX36, DDX1, DDX21 and TICAM1; this complex exists in resting cells with or without poly(I:C) RNA ligand stimulation (PubMed:21703541).
Interacts (via TIR domain) with DDX21 (via C-terminus) (PubMed:21703541).
Interacts (via TIR domain) with DHX36 (via C-terminus) (PubMed:21703541).
Interacts with AZI2 and IRF7 (By similarity).
Interacts (when phosphorylated) with IRF3; following activation and phosphorylation on the pLxIS motif by TBK1, recruits IRF3 (By similarity).
Interacts with TICAM2 in TLR4 recruitment (By similarity). Interaction with PIAS4 inhibits the TICAM1-induced NF-kappa-B, IRF and IFNB1 activation (By similarity).
Interacts with IKBKB and IKBKE (By similarity). Interaction with SARM1 blocks TICAM1-dependent transcription factor activation (By similarity).
Interacts with TRAF3.
Interacts with TRAFD1.
Interacts with UBQLN1 (via UBA domain).
Interacts with TBK1, TRAF6 and RIPK1 and these interactions are enhanced in the presence of WDFY1 (By similarity).
Interacts (via the TIR domain) with TLR3 in response to poly(I:C) and this interaction is enhanced in the presence of WDFY1 (PubMed:25736436).
Interacts with TLR4 in response to poly(I:C) in a WDFY1-dependent manner (PubMed:25736436).
Interacts with WDFY1 in response to poly(I:C) (PubMed:25736436).
Interacts with TRIM56 (By similarity).
Interacts (via the TIR domain) with TLR5 (By similarity).
Interacts with TRIM8 (By similarity).
By similarity4 PublicationsBinary interactionsi
Hide detailsQ80UF7
With | #Exp. | IntAct |
---|---|---|
Btk [P35991] | 2 | EBI-3649271,EBI-625119 |
Cblb [Q3TTA7] | 2 | EBI-3649271,EBI-3649276 |
Tbk1 [Q9WUN2] | 2 | EBI-3649271,EBI-764193 |
Traf3 [Q60803] | 2 | EBI-3649271,EBI-520135 |
GO - Molecular functioni
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 223122, 9 interactors |
DIPi | DIP-60033N |
IntActi | Q80UF7, 6 interactors |
STRINGi | 10090.ENSMUSP00000055104 |
Miscellaneous databases
RNActi | Q80UF7, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 395 – 534 | TIRPROSITE-ProRule annotationAdd BLAST | 140 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 153 | TRIF-NTDBy similarityAdd BLAST | 153 | |
Regioni | 514 – 713 | Sufficient to induce apoptosisBy similarityAdd BLAST | 200 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 84 – 91 | TRAF6-bindingBy similarity | 8 | |
Motifi | 206 – 209 | pLxIS motifBy similarity | 4 | |
Motifi | 247 – 254 | TRAF6-bindingBy similarity | 8 | |
Motifi | 296 – 306 | TRAF6-bindingBy similarityAdd BLAST | 11 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 348 – 384 | Pro-richAdd BLAST | 37 | |
Compositional biasi | 602 – 679 | Pro-richAdd BLAST | 78 |
Domaini
Phylogenomic databases
eggNOGi | ENOG502RXF3, Eukaryota |
GeneTreei | ENSGT00940000162411 |
HOGENOMi | CLU_022539_0_0_1 |
InParanoidi | Q80UF7 |
OMAi | YNFVVLH |
OrthoDBi | 435629at2759 |
PhylomeDBi | Q80UF7 |
TreeFami | TF336953 |
Family and domain databases
Gene3Di | 3.40.50.10140, 1 hit |
InterProi | View protein in InterPro IPR025735, RHIM_dom IPR017278, TICAM1 IPR035897, Toll_tir_struct_dom_sf IPR040886, TRIF_N |
PANTHERi | PTHR47230, PTHR47230, 1 hit |
Pfami | View protein in Pfam PF12721, RHIM, 1 hit PF17798, TRIF-NTD, 1 hit |
PIRSFi | PIRSF037744, TIR_Ticam, 1 hit |
SUPFAMi | SSF52200, SSF52200, 1 hit |
PROSITEi | View protein in PROSITE PS50104, TIR, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MDNPGPSLRG AFGILGALER DRLTHLKHKL GSLCSGSQES KLLHAMVLLA
60 70 80 90 100
LGQDTEARVS LESLKMNTVA QLVAHQWADM ETTEGPEEPP DLSWTVARLY
110 120 130 140 150
HLLAEENLCP ASTRDMAYQV ALRDFASQGD HQLGQLQNEA WDRCSSDIKG
160 170 180 190 200
DPSGFQPLHS HQGSLQPPSA SPAVTRSQPR PIDTPDWSWG HTLHSTNSTA
210 220 230 240 250
SLASHLEISQ SPTLAFLSSH HGTHGPSKLC NTPLDTQEPQ LVPEGCQEPE
260 270 280 290 300
EISWPPSVET SVSLGLPHEI SVPEVSPEEA SPILPDALAA PDTSVHCPIE
310 320 330 340 350
CTELSTNSRS PLTSTTESVG KQWPITSQRS PQVPVGDDSL QNTTSSSPPA
360 370 380 390 400
QPPSLQASPK LPPSPLSSAS SPSSYPAPPT STSPVLDHSE TSDQKFYNFV
410 420 430 440 450
VIHARADEQV ALRIREKLET LGVPDGATFC EEFQVPGRGE LHCLQDAIDH
460 470 480 490 500
SGFTILLLTA SFDCSLSLHQ INHALMNSLT QSGRQDCVIP LLPLECSQAQ
510 520 530 540 550
LSPDTTRLLH SIVWLDEHSP IFARKVANTF KTQKLQAQRV RWKKAQEART
560 570 580 590 600
LKEQSIQLEA ERQNVAAISA AYTAYVHSYR AWQAEMNKLG VAFGKNLSLG
610 620 630 640 650
TPTPSWPGCP QPIPSHPQGG TPVFPYSPQP PSFPQPPCFP QPPSFPQPPS
660 670 680 690 700
FPLPPVSSPQ SQSFPSASSP APQTPGPQPL IIHHAQMVQL GVNNHMWGHT
710 720 730
GAQSSDDKTE CSENPCMGPL TDQGEPLLET PE
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 227 | S → G in BAE29344 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 351 – 435 | Missing in AAH62191 (PubMed:15489334).CuratedAdd BLAST | 85 | |
Sequence conflicti | 435 | V → A in BAE25531 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 513 | V → A in BAE29344 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 628 – 639 | Missing in AAH33406 (PubMed:15489334).CuratedAdd BLAST | 12 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB091053 mRNA Translation: BAC55581.1 AK143766 mRNA Translation: BAE25531.1 AK150150 mRNA Translation: BAE29344.1 AK155245 mRNA Translation: BAE33144.1 BC033406 mRNA Translation: AAH33406.1 Sequence problems. BC037048 mRNA Translation: AAH37048.1 Different initiation. BC062191 mRNA Translation: AAH62191.1 Frameshift. BC094338 mRNA Translation: AAH94338.1 |
CCDSi | CCDS28900.1 |
RefSeqi | NP_778154.1, NM_174989.4 |
Genome annotation databases
Ensembli | ENSMUST00000058136; ENSMUSP00000055104; ENSMUSG00000047123 |
GeneIDi | 106759 |
KEGGi | mmu:106759 |
UCSCi | uc008dbm.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB091053 mRNA Translation: BAC55581.1 AK143766 mRNA Translation: BAE25531.1 AK150150 mRNA Translation: BAE29344.1 AK155245 mRNA Translation: BAE33144.1 BC033406 mRNA Translation: AAH33406.1 Sequence problems. BC037048 mRNA Translation: AAH37048.1 Different initiation. BC062191 mRNA Translation: AAH62191.1 Frameshift. BC094338 mRNA Translation: AAH94338.1 |
CCDSi | CCDS28900.1 |
RefSeqi | NP_778154.1, NM_174989.4 |
3D structure databases
SMRi | Q80UF7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 223122, 9 interactors |
DIPi | DIP-60033N |
IntActi | Q80UF7, 6 interactors |
STRINGi | 10090.ENSMUSP00000055104 |
PTM databases
PhosphoSitePlusi | Q80UF7 |
Proteomic databases
EPDi | Q80UF7 |
PaxDbi | Q80UF7 |
PRIDEi | Q80UF7 |
ProteomicsDBi | 263143 |
Protocols and materials databases
Antibodypediai | 11536, 350 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000058136; ENSMUSP00000055104; ENSMUSG00000047123 |
GeneIDi | 106759 |
KEGGi | mmu:106759 |
UCSCi | uc008dbm.2, mouse |
Organism-specific databases
CTDi | 148022 |
MGIi | MGI:2147032, Ticam1 |
Phylogenomic databases
eggNOGi | ENOG502RXF3, Eukaryota |
GeneTreei | ENSGT00940000162411 |
HOGENOMi | CLU_022539_0_0_1 |
InParanoidi | Q80UF7 |
OMAi | YNFVVLH |
OrthoDBi | 435629at2759 |
PhylomeDBi | Q80UF7 |
TreeFami | TF336953 |
Enzyme and pathway databases
Reactomei | R-MMU-140534, Caspase activation via Death Receptors in the presence of ligand R-MMU-166166, MyD88-independent TLR4 cascade R-MMU-2562578, TRIF-mediated programmed cell death R-MMU-936964, Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon R-MMU-937041, IKK complex recruitment mediated by RIP1 R-MMU-937072, TRAF6-mediated induction of TAK1 complex within TLR4 complex R-MMU-975163, IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation |
Miscellaneous databases
BioGRID-ORCSi | 106759, 3 hits in 54 CRISPR screens |
PROi | PR:Q80UF7 |
RNActi | Q80UF7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000047123, Expressed in intestine and 176 other tissues |
Genevisiblei | Q80UF7, MM |
Family and domain databases
Gene3Di | 3.40.50.10140, 1 hit |
InterProi | View protein in InterPro IPR025735, RHIM_dom IPR017278, TICAM1 IPR035897, Toll_tir_struct_dom_sf IPR040886, TRIF_N |
PANTHERi | PTHR47230, PTHR47230, 1 hit |
Pfami | View protein in Pfam PF12721, RHIM, 1 hit PF17798, TRIF-NTD, 1 hit |
PIRSFi | PIRSF037744, TIR_Ticam, 1 hit |
SUPFAMi | SSF52200, SSF52200, 1 hit |
PROSITEi | View protein in PROSITE PS50104, TIR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TCAM1_MOUSE | |
Accessioni | Q80UF7Primary (citable) accession number: Q80UF7 Secondary accession number(s): Q3UDB7 Q8JZV0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 5, 2008 |
Last sequence update: | June 1, 2003 | |
Last modified: | April 7, 2021 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot