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Protein

Ubiquitin carboxyl-terminal hydrolase 8

Gene

Usp8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1 (By similarity). Deubiquitinates BACE1 which inhibits BACE1 lysosomal degradation and modulates BACE-mediated APP cleavage and amyloid-beta formation (By similarity).By similarity9 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication EC:3.4.19.12

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei748Nucleophile1
Active sitei1029Proton acceptorPROSITE-ProRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processCell cycle, Ubl conjugation pathway

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-MMU-1358803 Downregulation of ERBB2:ERBB3 signaling
R-MMU-4641263 Regulation of FZD by ubiquitination
R-MMU-5689880 Ub-specific processing proteases
R-MMU-6807004 Negative regulation of MET activity

Protein family/group databases

MEROPS protease database

More...
MEROPSi
C19.011

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 8Curated (EC:3.4.19.121 Publication)
Alternative name(s):
Deubiquitinating enzyme 8
Ubiquitin isopeptidase Y
Short name:
mUBPy
Ubiquitin thioesterase 8
Ubiquitin-specific-processing protease 8
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Usp8Imported
Synonyms:Kiaa0055, Ubpy
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 2

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...
MGIi
MGI:1934029 Usp8

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endosome, Membrane, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi405P → A: Abolishes interaction with the SH3 domain of STAM2. 1 Publication1
Mutagenesisi406Q → A: Does not affect interaction with the SH3 domain of STAM2. 1 Publication1
Mutagenesisi407V → A: Reduces interaction with the SH3 domain of STAM2. 1 Publication1
Mutagenesisi408D → A: Reduces interaction with the SH3 domain of STAM2. 1 Publication1
Mutagenesisi409R → A: Abolishes interaction with the SH3 domain of STAM2. 1 Publication1
Mutagenesisi410T → A: Does not affect interaction with the SH3 domain of STAM2. 1 Publication1
Mutagenesisi411K → A: Does not affect interaction with the SH3 domain of STAM2. 1 Publication1
Mutagenesisi412K → A: Abolishes interaction with the SH3 domain of STAM2. 1 Publication1
Mutagenesisi413P → A: Abolishes interaction with the SH3 domain of STAM2. 1 Publication1
Mutagenesisi680S → A: Abolishes the interaction with YWHAE and leads to accumulation in the nucleus. 2 Publications1
Mutagenesisi748C → A: Impairs deubiquitination of EPS15, RNF128 and RNF41. 4 Publications1
Mutagenesisi907T → A: Reduces stabilization activity on RNF41. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000806281 – 1080Ubiquitin carboxyl-terminal hydrolase 8Add BLAST1080

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei160PhosphoserineBy similarity1
Modified residuei392PhosphoserineBy similarity1
Modified residuei446PhosphoserineBy similarity1
Modified residuei569PhosphothreonineBy similarity1
Modified residuei680Phosphoserine2 Publications1
Modified residuei681PhosphoserineBy similarity1
Modified residuei907Phosphothreonine1 Publication1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylation of Ser-680 is essential for interaction with YWHAE and for cytosol localization. Undergoes dephosphorylation at Ser-680 in the M phase. Tyrosine-phosphorylated in its N-terminal half in an EGFR-dependent manner.4 Publications
Ubiquitinated. Inactive form is mostly monoubiquitinated, but polyubiquitination happens too. Ubiquitination is increased in EGF-stimulated cells. Ubiquitination of active form is undetectable, suggesting a possibility that USP8 deubiquitinates itself, thereby regulating its own function.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
Q80U87

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q80U87

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q80U87

PRoteomics IDEntifications database

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PRIDEi
Q80U87

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

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iPTMneti
Q80U87

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
Q80U87

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Highly expressed in testis. Expressed at intermediate level in brain.2 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSMUSG00000027363 Expressed in 278 organ(s), highest expression level in testis

CleanEx database of gene expression profiles

More...
CleanExi
MM_USP8

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
Q80U87 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
Q80U87 MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Forms a ternary complex with RNF128 and OTUB1. Interacts (via C-terminal UCH catalytic domain) with OTUB1 isoform 1. Interacts with STAM2 (via SH3 domain). Interacts with DNAJB3, EGFR, EPS15, RASGRF1, RNF41, YWHAE, YWHAG and YWHAZ. Interacts with NBR1, RASGRF1, RNF41 and IST1 (By similarity). Associates with the ESCRT-0 complex and with microtubules. Interacts with BIRC6/bruce and KIF23/MKLP1.By similarity9 Publications

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

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BioGridi
220000, 10 interactors

Protein interaction database and analysis system

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IntActi
Q80U87, 7 interactors

Molecular INTeraction database

More...
MINTi
Q80U87

STRING: functional protein association networks

More...
STRINGi
10090.ENSMUSP00000106046

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11080
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

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ProteinModelPortali
Q80U87

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q80U87

Database of comparative protein structure models

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ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini33 – 116MITAdd BLAST84
Domaini195 – 313RhodanesePROSITE-ProRule annotationAdd BLAST119
Domaini739 – 1071USPAdd BLAST333

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi405 – 413SH3-binding9

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The MIT domain is required for endosomal localization, CHMP1B-binding, maintenance of ESCRT-0 stability and EGFR degradation.By similarity
The rhodanese domain is sufficient for RNF41-binding.

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Keywords - Domaini

SH3-binding

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG1868 Eukaryota
ENOG410XP8T LUCA

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000157542

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

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HOGENOMi
HOG000231497

The HOVERGEN Database of Homologous Vertebrate Genes

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HOVERGENi
HBG012631

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q80U87

KEGG Orthology (KO)

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KOi
K11839

Identification of Orthologs from Complete Genome Data

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OMAi
VIMKALW

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
3.40.250.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR001394 Peptidase_C19_UCH
IPR001763 Rhodanese-like_dom
IPR036873 Rhodanese-like_dom_sf
IPR015063 USP8_dimer
IPR018200 USP_CS
IPR028889 USP_dom

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00581 Rhodanese, 1 hit
PF00443 UCH, 1 hit
PF08969 USP8_dimer, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00450 RHOD, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF52821 SSF52821, 1 hit
SSF54001 SSF54001, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50206 RHODANESE_3, 1 hit
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q80U87-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPAVASVPKE LYLSSSLKDL NKKTEVKPEK TSTKNYIHSA QKIFKTAEEC
60 70 80 90 100
RLDRDEERAY VLYMKYVAVY NLIKKRPDFK QQQDYYLSIL GPANIKKAIE
110 120 130 140 150
EAERLSESLK LRYEEAEVRK QLEEKDRREE EQLQQQKRQE MGREDSGAAA
160 170 180 190 200
KRSVENLLDS KTKTQRINGE KSEGAAAAER GAITAKELYT MMMDKNTSLI
210 220 230 240 250
IMDARKIQDY QHSCILDSLS VPEEAISPGV TASWIEANLS DDSKDTWKKR
260 270 280 290 300
GSVDYVVLLD WFSSAKDLLL GTTLRSLKDA LFKWESKTVL RHEPLVLEGG
310 320 330 340 350
YENWLLCYPQ FTTNAKVTPP PRSRAEEVSV SLDFTYPSLE EPVPSKLPTQ
360 370 380 390 400
MPPPPIETNE KALLVTDQDE KLRLSTQPAL AGPGAAPRAE ASPIIQPAPA
410 420 430 440 450
TKSVPQVDRT KKPSVKLPED HRIKSENTDQ SGRVLSDRST KPVFPSPTTM
460 470 480 490 500
LTDEEKARIH QETALLMEKN KQEKELWDKQ QKEQKEKLRR EEQERKAGKT
510 520 530 540 550
QDADERDSTE NQHKAKDGQE KKDSKQTKTE DRELSADGAQ EATGTQRQSK
560 570 580 590 600
SEHEASDAKV PVEGKRCPTS EAQKRPADVS PASVSGELNA GKAQREPLTR
610 620 630 640 650
ARSEEMGRIV PGLPLGWAKF LDPITGTFRY YHSPTNTVHM YPPEMAPSSA
660 670 680 690 700
PPSTPPTHKV KPQVPAERDR EPSKLKRSYS SPDITQALQE EEKRRPAVTP
710 720 730 740 750
MVNRENKPPC YPKAEISRLS ASQIRNLNPV FGGSGPALTG LRNLGNTCYM
760 770 780 790 800
NSILQCLCNA PHLADYFNRN CYQDDINRSN LLGHKGEVAE EFGIIMKALW
810 820 830 840 850
TGQYRYISPK DFKVTIGKIN DQFAGSSQQD SQELLLFLMD GLHEDLNKAD
860 870 880 890 900
NRKRHKEENN EHLDDLQAAE HAWQKHKQLN ESIIVALFQG QFKSTVQCLT
910 920 930 940 950
CRRRSRTFEA FMYLSLPLAS TSKCTLQDCL RLFSKEEKLT DNNRFYCSHC
960 970 980 990 1000
RARRDSLKKI EIWKLPPVLL VHLKRFSYDG RWKQKLQTSV DFPLENLDLS
1010 1020 1030 1040 1050
QYVIGPKNSL KKYNLFSVSN HYGGLDGGHY TAYCKNAARQ RWFKFDDHEV
1060 1070 1080
SDISVSSVRS SAAYILFYTS LGPRITDVAT
Length:1,080
Mass (Da):122,611
Last modified:March 1, 2004 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i7F458E3B1AF0B7FA
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2AI52A2AI52_MOUSE
Ubiquitin carboxyl-terminal hydrola...
Usp8
1,091Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence BAC65477 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti139Q → P in AAD38869 (PubMed:11500497).Curated1
Sequence conflicti151K → R in BAB18534 (PubMed:10982817).Curated1
Sequence conflicti349T → A in BAB18534 (PubMed:10982817).Curated1
Sequence conflicti370E → G in BAB18534 (PubMed:10982817).Curated1
Sequence conflicti557D → N in BAB18534 (PubMed:10982817).Curated1
Sequence conflicti576P → L in BAB18534 (PubMed:10982817).Curated1
Sequence conflicti837F → S in BAB18534 (PubMed:10982817).Curated1
Sequence conflicti865 – 873DLQAAEHAW → EPAGGRARL in BAB18534 (PubMed:10982817).Curated9
Sequence conflicti885 – 886VA → RL in BAB18534 (PubMed:10982817).Curated2
Sequence conflicti894S → A in BAB18534 (PubMed:10982817).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
AB045709 mRNA Translation: BAB18534.1
AF057146 mRNA Translation: AAD38869.1
AK122195 mRNA Translation: BAC65477.1 Different initiation.
AL732330 Genomic DNA No translation available.
BC027052 mRNA Translation: AAH27052.1
BC050947 mRNA Translation: AAH50947.1
BC061465 mRNA Translation: AAH61465.1
BC066126 mRNA Translation: AAH66126.1

The Consensus CDS (CCDS) project

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CCDSi
CCDS16687.1

NCBI Reference Sequences

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RefSeqi
NP_062703.2, NM_019729.3

UniGene gene-oriented nucleotide sequence clusters

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UniGenei
Mm.272629
Mm.490069

Genome annotation databases

Ensembl eukaryotic genome annotation project

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Ensembli
ENSMUST00000028841; ENSMUSP00000028841; ENSMUSG00000027363

Database of genes from NCBI RefSeq genomes

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GeneIDi
84092

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
mmu:84092

UCSC genome browser

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UCSCi
uc008meb.2 mouse

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB045709 mRNA Translation: BAB18534.1
AF057146 mRNA Translation: AAD38869.1
AK122195 mRNA Translation: BAC65477.1 Different initiation.
AL732330 Genomic DNA No translation available.
BC027052 mRNA Translation: AAH27052.1
BC050947 mRNA Translation: AAH50947.1
BC061465 mRNA Translation: AAH61465.1
BC066126 mRNA Translation: AAH66126.1
CCDSiCCDS16687.1
RefSeqiNP_062703.2, NM_019729.3
UniGeneiMm.272629
Mm.490069

3D structure databases

Select the link destinations:

Protein Data Bank Europe

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PDBei

Protein Data Bank RCSB

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RCSB PDBi

Protein Data Bank Japan

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PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UJ0X-ray1.70B699-709[»]
ProteinModelPortaliQ80U87
SMRiQ80U87
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220000, 10 interactors
IntActiQ80U87, 7 interactors
MINTiQ80U87
STRINGi10090.ENSMUSP00000106046

Protein family/group databases

MEROPSiC19.011

PTM databases

iPTMnetiQ80U87
PhosphoSitePlusiQ80U87

Proteomic databases

EPDiQ80U87
MaxQBiQ80U87
PaxDbiQ80U87
PRIDEiQ80U87

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028841; ENSMUSP00000028841; ENSMUSG00000027363
GeneIDi84092
KEGGimmu:84092
UCSCiuc008meb.2 mouse

Organism-specific databases

Comparative Toxicogenomics Database

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CTDi
9101
MGIiMGI:1934029 Usp8

Rodent Unidentified Gene-Encoded large proteins database

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Rougei
Search...

Phylogenomic databases

eggNOGiKOG1868 Eukaryota
ENOG410XP8T LUCA
GeneTreeiENSGT00940000157542
HOGENOMiHOG000231497
HOVERGENiHBG012631
InParanoidiQ80U87
KOiK11839
OMAiVIMKALW

Enzyme and pathway databases

ReactomeiR-MMU-1358803 Downregulation of ERBB2:ERBB3 signaling
R-MMU-4641263 Regulation of FZD by ubiquitination
R-MMU-5689880 Ub-specific processing proteases
R-MMU-6807004 Negative regulation of MET activity

Miscellaneous databases

Protein Ontology

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PROi
PR:Q80U87

The Stanford Online Universal Resource for Clones and ESTs

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SOURCEi
Search...

Gene expression databases

BgeeiENSMUSG00000027363 Expressed in 278 organ(s), highest expression level in testis
CleanExiMM_USP8
ExpressionAtlasiQ80U87 baseline and differential
GenevisibleiQ80U87 MM

Family and domain databases

Gene3Di3.40.250.10, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR001394 Peptidase_C19_UCH
IPR001763 Rhodanese-like_dom
IPR036873 Rhodanese-like_dom_sf
IPR015063 USP8_dimer
IPR018200 USP_CS
IPR028889 USP_dom
PfamiView protein in Pfam
PF00581 Rhodanese, 1 hit
PF00443 UCH, 1 hit
PF08969 USP8_dimer, 1 hit
SMARTiView protein in SMART
SM00450 RHOD, 1 hit
SUPFAMiSSF52821 SSF52821, 1 hit
SSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50206 RHODANESE_3, 1 hit
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

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ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiUBP8_MOUSE
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q80U87
Secondary accession number(s): A2AI53
, Q80YP2, Q8R0D3, Q9EQU1, Q9WVP5
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: December 5, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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