UniProtKB - Q80U16 (RIPR2_MOUSE)
Rho family-interacting cell polarization regulator 2
Ripor2
Functioni
GO - Molecular functioni
- 14-3-3 protein binding Source: UniProtKB
- identical protein binding Source: UniProtKB
GO - Biological processi
- auditory receptor cell stereocilium organization Source: UniProtKB
- cell adhesion Source: UniProtKB-KW
- cellular response to chemokine Source: UniProtKB
- cellular response to mechanical stimulus Source: UniProtKB
- chemotaxis Source: UniProtKB-KW
- establishment of protein localization Source: UniProtKB
- negative regulation of cell adhesion Source: UniProtKB
- negative regulation of establishment of T cell polarity Source: UniProtKB
- negative regulation of protein localization to cell leading edge Source: UniProtKB
- negative regulation of Rho guanyl-nucleotide exchange factor activity Source: UniProtKB
- negative regulation of Rho protein signal transduction Source: UniProtKB
- negative regulation of T cell migration Source: UniProtKB
- negative regulation of T cell proliferation Source: MGI
- positive regulation of cell cycle arrest Source: MGI
- positive regulation of filopodium assembly Source: UniProtKB
- positive regulation of myoblast differentiation Source: UniProtKB
- positive regulation of myoblast fusion Source: UniProtKB
- positive regulation of neutrophil chemotaxis Source: UniProtKB
- positive regulation of neutrophil extravasation Source: UniProtKB
- protein homooligomerization Source: UniProtKB
- regulation of establishment of cell polarity Source: UniProtKB
- regulation of mitotic spindle assembly Source: MGI
- sensory perception of sound Source: UniProtKB
- skeletal muscle fiber development Source: InterPro
Keywordsi
Molecular function | Signal transduction inhibitor |
Biological process | Cell adhesion, Chemotaxis, Differentiation, Hearing, Myogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: Rho family-interacting cell polarization regulator 2By similarity |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2444879, Ripor2 |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
Plasma membrane
- stereocilium membrane By similarity
- Apical cell membrane By similarity
Other locations
- Cytoplasm By similarity
- filopodium By similarity
- stereocilium 1 Publication
Note: Localized in the cytoplasm in cells undergoing mitosis (By similarity). Colocalized with F-actin (By similarity). Accumulates at the leading edge of polarized neutrophils in a chemokine-dependent manner (By similarity). Localized with RHOC within the basal domain of hair cell stereocilia, near the taper region (PubMed:27269051). Detected in punctate pattern forming a circumferential ring at the stereocilia base (PubMed:27269051). Localized to the apical stereocilia of inner and outer hair cells (By similarity). Not detected as a membrane-associated protein in stereocilia (PubMed:27269051).By similarity1 Publication
Cytoskeleton
- cytoskeleton Source: UniProtKB
Plasma Membrane
- apical plasma membrane Source: MGI
- stereocilium membrane Source: MGI
Other locations
- cytoplasm Source: UniProtKB
- filopodium Source: UniProtKB
- stereocilium Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 176 – 177 | RL → AA: Inhibits interaction with RHOC. Reduces homooligomerization activity. Inhibits RHOC-dependent homooligomerization activity. Abolishes ability to rescue the morphological hair bundle defect in hair cell of RIPOR2-deficient mice. 1 Publication | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000289115 | 1 – 1078 | Rho family-interacting cell polarization regulator 2Add BLAST | 1078 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 46 | PhosphoserineCombined sources | 1 | |
Modified residuei | 62 | PhosphoserineBy similarity | 1 | |
Modified residuei | 366 | PhosphoserineCombined sources | 1 | |
Modified residuei | 582 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q80U16 |
jPOSTi | Q80U16 |
PaxDbi | Q80U16 |
PRIDEi | Q80U16 |
PTM databases
iPTMneti | Q80U16 |
PhosphoSitePlusi | Q80U16 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000036006, Expressed in blood and 269 other tissues |
Genevisiblei | Q80U16, MM |
Interactioni
Subunit structurei
Homooligomer; homooligomerization is regulated by RHOC and leads to the formation of concatemers through the association of N- and C-termini (PubMed:27269051).
Interacts (phosphorylated form) with 14-3-3 proteins; these interactions occur during myogenic cell differentiation and also induces T cell proliferation arrest (By similarity).
Interacts (phosphorylated form) with HDAC6; this interaction occurs during early myogenic differentiation, prevents HDAC6 to deacetylate tubulin and also induces T cell proliferation arrest (By similarity).
Interacts with DYSF; this interaction occurs during early myogenic differentiation (PubMed:24687993).
Interacts with MYOF (By similarity).
Interacts (via active GTP- or inactive GDP-bound forms) with RHOA; this interaction is direct, blocks the loading of GTP to RHOA and decreases upon chemokine CCL19 stimulation in primary T lymphocytes (PubMed:27269051).
Interacts with RHOC (PubMed:27269051).
Interacts (via phosphorylated form) with YWHAB; this interaction occurs in a chemokine-dependent manner and does not compete for binding of RIPOR2 with RHOA nor blocks inhibition of RIPOR2-mediated RHOA activity (By similarity).
Interacts with YWHAE (By similarity).
Interacts with YWHAQ (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- 14-3-3 protein binding Source: UniProtKB
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 228735, 2 interactors |
STRINGi | 10090.ENSMUSP00000106013 |
Miscellaneous databases
RNActi | Q80U16, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 80 – 138 | Involved in cell filopodia formationBy similarityAdd BLAST | 59 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 108 – 137 | Sequence analysisAdd BLAST | 30 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QQ7T, Eukaryota |
GeneTreei | ENSGT00940000153717 |
HOGENOMi | CLU_432085_0_0_1 |
InParanoidi | Q80U16 |
OrthoDBi | 1121546at2759 |
PhylomeDBi | Q80U16 |
TreeFami | TF329332 |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR031780, FAM65_N IPR033035, FAM65B IPR026136, RIPOR3 |
PANTHERi | PTHR15829, PTHR15829, 1 hit PTHR15829:SF2, PTHR15829:SF2, 1 hit |
Pfami | View protein in Pfam PF15903, PL48, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
s (7+)i Sequence
Sequence statusi: Complete.
This entry describes 7 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 7 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPPGTKRLRA LGAFSAGLPT RLPEIMLVGS QSFSPGGPNG IIRSQSFAGF
60 70 80 90 100
SGLQERRSRC NSFIENASAL KKPQAKLKKM HNLGHKNNNT PKEPQPKRVE
110 120 130 140 150
EVYRALKNGL DEYLEFHQTE LDKLTAQLKD MKRNSRLGVL YDLDKQIKTI
160 170 180 190 200
ERYMRRLEFH ISKVDELYEA YCIQRRLQDG ASKMKQAFAT SPASKAARES
210 220 230 240 250
LSEINRSYKE YTENMCAIEA ELESLLGEFS IKMKGLAGFA RLCPGDQYEI
260 270 280 290 300
FMKYGRQRWK LKGKIEVNGK QSWDGAETVF LPLIVGFISI KVTELKGLAT
310 320 330 340 350
HILVGSVTCE TKELFAARPQ VVAVDINDLG TIKLNLEITW YPFDVEDTTP
360 370 380 390 400
SSGPGNKTAA LQRRMSMYSQ GTPETPTFKD QSFFRWLRLS VLSALRDTFF
410 420 430 440 450
ATLHHNHSVG DLPSLSLNPK ALLEFYSNLP DDIFESGKAA EEKRPLSLSF
460 470 480 490 500
SDLQDGDCVF TSSSATSPSS SHSAHPEITI TPAELTHSSL SSQNEGTEDS
510 520 530 540 550
SSASSRNSLG EDHEPKSHPK SDTVEPGKPG VATRSGTESL FLESSVAEAL
560 570 580 590 600
LQESDEASEL KPVELDTFEG NITKQLVKRL TSAEGPVTTD KLFFEGSVGS
610 620 630 640 650
ESEAGRSFLD GSLEDAFNGL FLALDPHKKQ YKEFQDLNQE VTHLDDVLKC
660 670 680 690 700
KPAGSRSRSS SLSLTVESAL ESFDFLNTSD FDEEEEDGDD VCHVGGGADS
710 720 730 740 750
VFSDTETEKS GYRSVHPEAR GHLSEALTED TGVGTSVAGS PLPLTTGNES
760 770 780 790 800
LDITIVKHLQ YCTQLIQQIV FSSKTPFVAR SLLEKLSRQV LVMQKLAAVS
810 820 830 840 850
DENLGNITSV VEAIPEFHKK LSLLAFWTKC CSPSGVYHSS AARLIKQLEA
860 870 880 890 900
SFARSINKDY PGLAEPVFRT LVSQILDRAE PLLSSSLSSE VITVFQYYSF
910 920 930 940 950
FTSHGVSDLE TYLGQLTRQV AMVQTLQSLR DEKLLQTMSD LAPSNLPAQQ
960 970 980 990 1000
EVLRTLALLL TRDDNEVSEA VTLYLAAASK NEHFREKALL YYCEALTKAN
1010 1020 1030 1040 1050
LQLQKAACLA LKSLEATESI KMLVTLCQSD TEEIRTVASE TLLSLGEDGR
1060 1070
LAYEQLDKFP RDCVKVGGRH GTEVATAF
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA6PW28 | A6PW28_MOUSE | Rho family-interacting cell polariz... | Ripor2 Fam65b, RP23-461O24.1 | 1,053 | Annotation score: | ||
A0A5F8MPY8 | A0A5F8MPY8_MOUSE | Rho family-interacting cell polariz... | Ripor2 | 639 | Annotation score: | ||
F2Z3V4 | F2Z3V4_MOUSE | Rho family-interacting cell polariz... | Ripor2 Fam65b | 40 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 534 | R → S in BAC65551 (PubMed:12693553).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_025907 | 1 – 25 | Missing in isoform 7. 1 PublicationAdd BLAST | 25 | |
Alternative sequenceiVSP_025908 | 1 – 16 | MPPGT…GAFSA → MQFLDPEDLLDEEDDIFGE in isoform 5 and isoform 6. 1 PublicationAdd BLAST | 16 | |
Alternative sequenceiVSP_025909 | 385 – 426 | Missing in isoform 5, isoform 6 and isoform 7. 1 PublicationAdd BLAST | 42 | |
Alternative sequenceiVSP_025910 | 385 – 389 | RWLRL → VGNGT in isoform 3. 1 Publication | 5 | |
Alternative sequenceiVSP_025911 | 385 – 388 | RWLR → VSSV in isoform 4. 1 Publication | 4 | |
Alternative sequenceiVSP_025912 | 389 – 1078 | Missing in isoform 4. 1 PublicationAdd BLAST | 690 | |
Alternative sequenceiVSP_025913 | 390 – 1078 | Missing in isoform 3. 1 PublicationAdd BLAST | 689 | |
Alternative sequenceiVSP_025914 | 650 – 672 | CKPAG…SALES → DAKHLEDQRLNDAASRMEIT EGE in isoform 2, isoform 5 and isoform 7. 1 PublicationAdd BLAST | 23 | |
Alternative sequenceiVSP_025915 | 650 – 668 | CKPAG…LTVES → VSTFLGKKKKSDLVWKNAS in isoform 6. 1 PublicationAdd BLAST | 19 | |
Alternative sequenceiVSP_025916 | 669 – 1078 | Missing in isoform 6. 1 PublicationAdd BLAST | 410 | |
Alternative sequenceiVSP_025917 | 673 – 1078 | Missing in isoform 2, isoform 5 and isoform 7. 1 PublicationAdd BLAST | 406 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK122269 mRNA Translation: BAC65551.1 Different initiation. AK031928 mRNA Translation: BAC27606.1 AK036452 mRNA Translation: BAC29436.1 AK039271 mRNA Translation: BAC30301.1 AK040887 mRNA Translation: BAC30732.1 AK041665 mRNA Translation: BAC31025.1 AK163880 mRNA Translation: BAE37527.1 AL591851, AL513014 Genomic DNA Translation: CAI25708.2 AL513014, AL589699 Genomic DNA Translation: CAI25774.2 AL513014, AL591851 Genomic DNA Translation: CAI25775.2 AL589699, AL513014 Genomic DNA Translation: CAI26080.2 AL513014 Genomic DNA Translation: CAO77914.1 AL513014, AL589699 Genomic DNA Translation: CAO77916.1 |
CCDSi | CCDS26377.2 [Q80U16-5] CCDS36622.1 [Q80U16-1] CCDS36623.1 [Q80U16-2] |
RefSeqi | NP_001073850.1, NM_001080381.1 [Q80U16-2] NP_001273029.1, NM_001286100.1 NP_001273030.1, NM_001286101.1 [Q80U16-7] NP_083955.1, NM_029679.2 [Q80U16-1] NP_848773.3, NM_178658.5 [Q80U16-5] |
Genome annotation databases
Ensembli | ENSMUST00000038477; ENSMUSP00000043663; ENSMUSG00000036006 [Q80U16-2] ENSMUST00000058009; ENSMUSP00000051342; ENSMUSG00000036006 [Q80U16-3] ENSMUST00000091694; ENSMUSP00000089286; ENSMUSG00000036006 [Q80U16-5] ENSMUST00000110384; ENSMUSP00000106013; ENSMUSG00000036006 [Q80U16-1] |
GeneIDi | 193385 |
KEGGi | mmu:193385 |
UCSCi | uc007pwa.2, mouse [Q80U16-6] uc007pwb.2, mouse [Q80U16-5] uc007pwc.1, mouse [Q80U16-4] uc007pwd.1, mouse [Q80U16-2] uc007pwe.1, mouse [Q80U16-1] uc011yxo.2, mouse [Q80U16-7] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK122269 mRNA Translation: BAC65551.1 Different initiation. AK031928 mRNA Translation: BAC27606.1 AK036452 mRNA Translation: BAC29436.1 AK039271 mRNA Translation: BAC30301.1 AK040887 mRNA Translation: BAC30732.1 AK041665 mRNA Translation: BAC31025.1 AK163880 mRNA Translation: BAE37527.1 AL591851, AL513014 Genomic DNA Translation: CAI25708.2 AL513014, AL589699 Genomic DNA Translation: CAI25774.2 AL513014, AL591851 Genomic DNA Translation: CAI25775.2 AL589699, AL513014 Genomic DNA Translation: CAI26080.2 AL513014 Genomic DNA Translation: CAO77914.1 AL513014, AL589699 Genomic DNA Translation: CAO77916.1 |
CCDSi | CCDS26377.2 [Q80U16-5] CCDS36622.1 [Q80U16-1] CCDS36623.1 [Q80U16-2] |
RefSeqi | NP_001073850.1, NM_001080381.1 [Q80U16-2] NP_001273029.1, NM_001286100.1 NP_001273030.1, NM_001286101.1 [Q80U16-7] NP_083955.1, NM_029679.2 [Q80U16-1] NP_848773.3, NM_178658.5 [Q80U16-5] |
3D structure databases
SMRi | Q80U16 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 228735, 2 interactors |
STRINGi | 10090.ENSMUSP00000106013 |
PTM databases
iPTMneti | Q80U16 |
PhosphoSitePlusi | Q80U16 |
Proteomic databases
EPDi | Q80U16 |
jPOSTi | Q80U16 |
PaxDbi | Q80U16 |
PRIDEi | Q80U16 |
Protocols and materials databases
Antibodypediai | 25358, 162 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000038477; ENSMUSP00000043663; ENSMUSG00000036006 [Q80U16-2] ENSMUST00000058009; ENSMUSP00000051342; ENSMUSG00000036006 [Q80U16-3] ENSMUST00000091694; ENSMUSP00000089286; ENSMUSG00000036006 [Q80U16-5] ENSMUST00000110384; ENSMUSP00000106013; ENSMUSG00000036006 [Q80U16-1] |
GeneIDi | 193385 |
KEGGi | mmu:193385 |
UCSCi | uc007pwa.2, mouse [Q80U16-6] uc007pwb.2, mouse [Q80U16-5] uc007pwc.1, mouse [Q80U16-4] uc007pwd.1, mouse [Q80U16-2] uc007pwe.1, mouse [Q80U16-1] uc011yxo.2, mouse [Q80U16-7] |
Organism-specific databases
CTDi | 9750 |
MGIi | MGI:2444879, Ripor2 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | ENOG502QQ7T, Eukaryota |
GeneTreei | ENSGT00940000153717 |
HOGENOMi | CLU_432085_0_0_1 |
InParanoidi | Q80U16 |
OrthoDBi | 1121546at2759 |
PhylomeDBi | Q80U16 |
TreeFami | TF329332 |
Miscellaneous databases
BioGRID-ORCSi | 193385, 0 hits in 17 CRISPR screens |
ChiTaRSi | Fam65b, mouse |
PROi | PR:Q80U16 |
RNActi | Q80U16, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036006, Expressed in blood and 269 other tissues |
Genevisiblei | Q80U16, MM |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR031780, FAM65_N IPR033035, FAM65B IPR026136, RIPOR3 |
PANTHERi | PTHR15829, PTHR15829, 1 hit PTHR15829:SF2, PTHR15829:SF2, 1 hit |
Pfami | View protein in Pfam PF15903, PL48, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RIPR2_MOUSE | |
Accessioni | Q80U16Primary (citable) accession number: Q80U16 Secondary accession number(s): A6PW01 Q8BJ54 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2007 |
Last sequence update: | May 29, 2007 | |
Last modified: | December 2, 2020 | |
This is version 115 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families