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Protein

Rho family-interacting cell polarization regulator 2

Gene

Ripor2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as an inhibitor of the small GTPase RHOA and plays several roles in the regulation of myoblast and hair cell differentiation, lymphocyte T proliferation and neutrophil polarization (PubMed:25588844, PubMed:27269051). Plays a role in fetal mononuclear myoblast differentiation by promoting filopodia and myotube formation (PubMed:17150207). Maintains naive T lymphocytes in a quiescent state and prevents chemokine-induced T lymphocyte responses, such as cell adhesion, polarization and migration (By similarity). Involved also in the regulation of neutrophil polarization, chemotaxis and adhesion (PubMed:25588844). Required for normal development of inner and outer hair cell stereocilia within the cochlea of the inner ear (PubMed:27269051). Plays a role for maintaining the structural organization of the basal domain of stereocilia (PubMed:27269051). Involved in mechanosensory hair cell function (PubMed:27269051). Required for normal hearing (PubMed:27269051).By similarity3 Publications

GO - Molecular functioni

  • 14-3-3 protein binding Source: UniProtKB
  • identical protein binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionSignal transduction inhibitor
Biological processCell adhesion, Chemotaxis, Differentiation, Hearing, Myogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Rho family-interacting cell polarization regulator 2By similarity
Gene namesi
Name:Ripor2By similarity
Synonyms:Fam65b, Kiaa0386
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2444879 Ripor2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are deaf at 4 weeks of age (PubMed:27269051). Show abnormal hair bundle morphology and polarity and stereociliary growth in the cochlea of the inner ear (PubMed:27269051). Display mislocalization of protein TPRN to the base of stereocilia (PubMed:27269051). Show reduced mechanotransduction currents in hair bundles of outer hair cells (PubMed:27269051). Mice show impaired neutrophil chemotaxis, increased neutrophil adhesion to endothelial cell and reduced neutrophil infiltration into inflamed peritonea (PubMed:25588844). Display increased chemokine-induced RHOA activity and mislocalization of myosin light chain MYL2 in neutrophils (PubMed:25588844).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi176 – 177RL → AA: Inhibits interaction with RHOC. Reduces homooligomerization activity. Inhibits RHOC-dependent homooligomerization activity. Abolishes ability to rescue the morphological hair bundle defect in hair cell of RIPOR2-deficient mice. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002891151 – 1078Rho family-interacting cell polarization regulator 2Add BLAST1078

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei46PhosphoserineCombined sources1
Modified residuei62PhosphoserineBy similarity1
Modified residuei366PhosphoserineCombined sources1
Modified residuei582PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated. Chemokine-induced phosphorylation in neutrophils occurs in a PKC- and AKT-dependent manner, resulting in RIPOR2 interaction with YWHAB and stabilization. Phosphorylated by PKCA, AKT1 and MAPKAPK1A; in vitro.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ80U16
PaxDbiQ80U16
PRIDEiQ80U16

PTM databases

iPTMnetiQ80U16
PhosphoSitePlusiQ80U16

Expressioni

Tissue specificityi

Expressed in the cochlea (PubMed:24958875). Expressed in inner hair cells and outer hair cells and Hensen's cells (at protein level) (PubMed:27269051). Expressed in the brain, cerebellum, spinal cord, retina, heart, spleen liver, kidney, bladder, muscle and lung ((PubMed:24958875), PubMed:27269051). Expressed in the cochlea of the inner ear ((PubMed:24958875), PubMed:27269051).2 Publications

Inductioni

Up-regulated during regenerating muscle tissue.1 Publication

Gene expression databases

BgeeiENSMUSG00000036006 Expressed in 249 organ(s), highest expression level in blood
CleanExiMM_6330500D04RIK
ExpressionAtlasiQ80U16 baseline and differential
GenevisibleiQ80U16 MM

Interactioni

Subunit structurei

Homooligomer; homooligomerization is regulated by RHOC and leads to the formation of concatemers through the association of N- and C-termini (PubMed:27269051). Interacts (phosphorylated form) with 14-3-3 proteins; these interactions occur during myogenic cell differentiation and also induces T cell proliferation arrest (By similarity). Interacts (phosphorylated form) with HDAC6; this interaction occurs during early myogenic differentiation, prevents HDAC6 to deacetylate tubulin and also induces T cell proliferation arrest (By similarity). Interacts with DYSF; this interaction occurs during early myogenic differentiation (PubMed:24687993). Interacts with MYOF (By similarity). Interacts (via active GTP- or inactive GDP-bound forms) with RHOA; this interaction is direct, blocks the loading of GTP to RHOA and decreases upon chemokine CCL19 stimulation in primary T lymphocytes (PubMed:27269051). Interacts with RHOC (PubMed:27269051). Interacts (via phosphorylated form) with YWHAB; this interaction occurs in a chemokine-dependent manner and does not compete for binding of RIPOR2 with RHOA nor blocks inhibition of RIPOR2-mediated RHOA activity (By similarity). Interacts with YWHAE (By similarity). Interacts with YWHAQ (By similarity).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228735, 1 interactor
STRINGi10090.ENSMUSP00000106013

Structurei

3D structure databases

SMRiQ80U16
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni80 – 138Involved in cell filopodia formationBy similarityAdd BLAST59

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili108 – 137Sequence analysisAdd BLAST30

Sequence similaritiesi

Belongs to the RIPOR family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IEN6 Eukaryota
ENOG410XR7R LUCA
GeneTreeiENSGT00490000043360
HOVERGENiHBG053834
InParanoidiQ80U16
OrthoDBiEOG091G018M
PhylomeDBiQ80U16
TreeFamiTF329332

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR031780 FAM65_N
IPR033035 FAM65B
IPR026136 RIPOR3
PANTHERiPTHR15829 PTHR15829, 1 hit
PTHR15829:SF2 PTHR15829:SF2, 1 hit
PfamiView protein in Pfam
PF15903 PL48, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit

Sequences (7+)i

Sequence statusi: Complete.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 7 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q80U16-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPPGTKRLRA LGAFSAGLPT RLPEIMLVGS QSFSPGGPNG IIRSQSFAGF
60 70 80 90 100
SGLQERRSRC NSFIENASAL KKPQAKLKKM HNLGHKNNNT PKEPQPKRVE
110 120 130 140 150
EVYRALKNGL DEYLEFHQTE LDKLTAQLKD MKRNSRLGVL YDLDKQIKTI
160 170 180 190 200
ERYMRRLEFH ISKVDELYEA YCIQRRLQDG ASKMKQAFAT SPASKAARES
210 220 230 240 250
LSEINRSYKE YTENMCAIEA ELESLLGEFS IKMKGLAGFA RLCPGDQYEI
260 270 280 290 300
FMKYGRQRWK LKGKIEVNGK QSWDGAETVF LPLIVGFISI KVTELKGLAT
310 320 330 340 350
HILVGSVTCE TKELFAARPQ VVAVDINDLG TIKLNLEITW YPFDVEDTTP
360 370 380 390 400
SSGPGNKTAA LQRRMSMYSQ GTPETPTFKD QSFFRWLRLS VLSALRDTFF
410 420 430 440 450
ATLHHNHSVG DLPSLSLNPK ALLEFYSNLP DDIFESGKAA EEKRPLSLSF
460 470 480 490 500
SDLQDGDCVF TSSSATSPSS SHSAHPEITI TPAELTHSSL SSQNEGTEDS
510 520 530 540 550
SSASSRNSLG EDHEPKSHPK SDTVEPGKPG VATRSGTESL FLESSVAEAL
560 570 580 590 600
LQESDEASEL KPVELDTFEG NITKQLVKRL TSAEGPVTTD KLFFEGSVGS
610 620 630 640 650
ESEAGRSFLD GSLEDAFNGL FLALDPHKKQ YKEFQDLNQE VTHLDDVLKC
660 670 680 690 700
KPAGSRSRSS SLSLTVESAL ESFDFLNTSD FDEEEEDGDD VCHVGGGADS
710 720 730 740 750
VFSDTETEKS GYRSVHPEAR GHLSEALTED TGVGTSVAGS PLPLTTGNES
760 770 780 790 800
LDITIVKHLQ YCTQLIQQIV FSSKTPFVAR SLLEKLSRQV LVMQKLAAVS
810 820 830 840 850
DENLGNITSV VEAIPEFHKK LSLLAFWTKC CSPSGVYHSS AARLIKQLEA
860 870 880 890 900
SFARSINKDY PGLAEPVFRT LVSQILDRAE PLLSSSLSSE VITVFQYYSF
910 920 930 940 950
FTSHGVSDLE TYLGQLTRQV AMVQTLQSLR DEKLLQTMSD LAPSNLPAQQ
960 970 980 990 1000
EVLRTLALLL TRDDNEVSEA VTLYLAAASK NEHFREKALL YYCEALTKAN
1010 1020 1030 1040 1050
LQLQKAACLA LKSLEATESI KMLVTLCQSD TEEIRTVASE TLLSLGEDGR
1060 1070
LAYEQLDKFP RDCVKVGGRH GTEVATAF
Length:1,078
Mass (Da):118,970
Last modified:May 29, 2007 - v2
Checksum:i207E1F6172144E2C
GO
Isoform 2 (identifier: Q80U16-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     650-672: CKPAGSRSRSSSLSLTVESALES → DAKHLEDQRLNDAASRMEITEGE
     673-1078: Missing.

Show »
Length:672
Mass (Da):74,628
Checksum:i8E924B06A47E3FBD
GO
Isoform 3 (identifier: Q80U16-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     385-389: RWLRL → VGNGT
     390-1078: Missing.

Show »
Length:389
Mass (Da):43,711
Checksum:i876770C69269A3A6
GO
Isoform 4 (identifier: Q80U16-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     385-388: RWLR → VSSV
     389-1078: Missing.

Show »
Length:388
Mass (Da):43,655
Checksum:iDDD12B5269A3A6B2
GO
Isoform 5 (identifier: Q80U16-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: MPPGTKRLRALGAFSA → MQFLDPEDLLDEEDDIFGE
     385-426: Missing.
     650-672: CKPAGSRSRSSSLSLTVESALES → DAKHLEDQRLNDAASRMEITEGE
     673-1078: Missing.

Show »
Length:633
Mass (Da):70,407
Checksum:i1CE9B5CE340351F8
GO
Isoform 6 (identifier: Q80U16-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: MPPGTKRLRALGAFSA → MQFLDPEDLLDEEDDIFGE
     385-426: Missing.
     650-668: CKPAGSRSRSSSLSLTVES → VSTFLGKKKKSDLVWKNAS
     669-1078: Missing.

Show »
Length:629
Mass (Da):69,915
Checksum:i4D1898FF789914F6
GO
Isoform 7 (identifier: Q80U16-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: Missing.
     385-426: Missing.
     650-672: CKPAGSRSRSSSLSLTVESALES → DAKHLEDQRLNDAASRMEITEGE
     673-1078: Missing.

Show »
Length:605
Mass (Da):67,177
Checksum:i05CFCB94CEB87FB6
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A6PW28A6PW28_MOUSE
Likely orthologue of H. sapiens chr...
Ripor2 6330500D04Rik, Fam65b, RP23-461O24.1, mCG_145700
1,053Annotation score:
F2Z3V4F2Z3V4_MOUSE
Rho family-interacting cell polariz...
Ripor2 Fam65b
40Annotation score:

Sequence cautioni

The sequence BAC65551 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti534R → S in BAC65551 (PubMed:12693553).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0259071 – 25Missing in isoform 7. 1 PublicationAdd BLAST25
Alternative sequenceiVSP_0259081 – 16MPPGT…GAFSA → MQFLDPEDLLDEEDDIFGE in isoform 5 and isoform 6. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_025909385 – 426Missing in isoform 5, isoform 6 and isoform 7. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_025910385 – 389RWLRL → VGNGT in isoform 3. 1 Publication5
Alternative sequenceiVSP_025911385 – 388RWLR → VSSV in isoform 4. 1 Publication4
Alternative sequenceiVSP_025912389 – 1078Missing in isoform 4. 1 PublicationAdd BLAST690
Alternative sequenceiVSP_025913390 – 1078Missing in isoform 3. 1 PublicationAdd BLAST689
Alternative sequenceiVSP_025914650 – 672CKPAG…SALES → DAKHLEDQRLNDAASRMEIT EGE in isoform 2, isoform 5 and isoform 7. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_025915650 – 668CKPAG…LTVES → VSTFLGKKKKSDLVWKNAS in isoform 6. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_025916669 – 1078Missing in isoform 6. 1 PublicationAdd BLAST410
Alternative sequenceiVSP_025917673 – 1078Missing in isoform 2, isoform 5 and isoform 7. 1 PublicationAdd BLAST406

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122269 mRNA Translation: BAC65551.1 Different initiation.
AK031928 mRNA Translation: BAC27606.1
AK036452 mRNA Translation: BAC29436.1
AK039271 mRNA Translation: BAC30301.1
AK040887 mRNA Translation: BAC30732.1
AK041665 mRNA Translation: BAC31025.1
AK163880 mRNA Translation: BAE37527.1
AL591851, AL513014 Genomic DNA Translation: CAI25708.2
AL513014, AL589699 Genomic DNA Translation: CAI25774.2
AL513014, AL591851 Genomic DNA Translation: CAI25775.2
AL589699, AL513014 Genomic DNA Translation: CAI26080.2
AL513014 Genomic DNA Translation: CAO77914.1
AL513014, AL589699 Genomic DNA Translation: CAO77916.1
CCDSiCCDS26377.2 [Q80U16-5]
CCDS36622.1 [Q80U16-1]
CCDS36623.1 [Q80U16-2]
RefSeqiNP_001073850.1, NM_001080381.1 [Q80U16-2]
NP_001273029.1, NM_001286100.1
NP_001273030.1, NM_001286101.1 [Q80U16-7]
NP_083955.1, NM_029679.2 [Q80U16-1]
NP_848773.3, NM_178658.5 [Q80U16-5]
UniGeneiMm.217319

Genome annotation databases

EnsembliENSMUST00000038477; ENSMUSP00000043663; ENSMUSG00000036006 [Q80U16-2]
ENSMUST00000058009; ENSMUSP00000051342; ENSMUSG00000036006 [Q80U16-3]
ENSMUST00000091694; ENSMUSP00000089286; ENSMUSG00000036006 [Q80U16-5]
ENSMUST00000110384; ENSMUSP00000106013; ENSMUSG00000036006 [Q80U16-1]
GeneIDi193385
KEGGimmu:193385
UCSCiuc007pwa.2 mouse [Q80U16-6]
uc007pwb.2 mouse [Q80U16-5]
uc007pwc.1 mouse [Q80U16-4]
uc007pwd.1 mouse [Q80U16-2]
uc007pwe.1 mouse [Q80U16-1]
uc011yxo.2 mouse [Q80U16-7]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122269 mRNA Translation: BAC65551.1 Different initiation.
AK031928 mRNA Translation: BAC27606.1
AK036452 mRNA Translation: BAC29436.1
AK039271 mRNA Translation: BAC30301.1
AK040887 mRNA Translation: BAC30732.1
AK041665 mRNA Translation: BAC31025.1
AK163880 mRNA Translation: BAE37527.1
AL591851, AL513014 Genomic DNA Translation: CAI25708.2
AL513014, AL589699 Genomic DNA Translation: CAI25774.2
AL513014, AL591851 Genomic DNA Translation: CAI25775.2
AL589699, AL513014 Genomic DNA Translation: CAI26080.2
AL513014 Genomic DNA Translation: CAO77914.1
AL513014, AL589699 Genomic DNA Translation: CAO77916.1
CCDSiCCDS26377.2 [Q80U16-5]
CCDS36622.1 [Q80U16-1]
CCDS36623.1 [Q80U16-2]
RefSeqiNP_001073850.1, NM_001080381.1 [Q80U16-2]
NP_001273029.1, NM_001286100.1
NP_001273030.1, NM_001286101.1 [Q80U16-7]
NP_083955.1, NM_029679.2 [Q80U16-1]
NP_848773.3, NM_178658.5 [Q80U16-5]
UniGeneiMm.217319

3D structure databases

SMRiQ80U16
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228735, 1 interactor
STRINGi10090.ENSMUSP00000106013

PTM databases

iPTMnetiQ80U16
PhosphoSitePlusiQ80U16

Proteomic databases

EPDiQ80U16
PaxDbiQ80U16
PRIDEiQ80U16

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038477; ENSMUSP00000043663; ENSMUSG00000036006 [Q80U16-2]
ENSMUST00000058009; ENSMUSP00000051342; ENSMUSG00000036006 [Q80U16-3]
ENSMUST00000091694; ENSMUSP00000089286; ENSMUSG00000036006 [Q80U16-5]
ENSMUST00000110384; ENSMUSP00000106013; ENSMUSG00000036006 [Q80U16-1]
GeneIDi193385
KEGGimmu:193385
UCSCiuc007pwa.2 mouse [Q80U16-6]
uc007pwb.2 mouse [Q80U16-5]
uc007pwc.1 mouse [Q80U16-4]
uc007pwd.1 mouse [Q80U16-2]
uc007pwe.1 mouse [Q80U16-1]
uc011yxo.2 mouse [Q80U16-7]

Organism-specific databases

CTDi9750
MGIiMGI:2444879 Ripor2
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IEN6 Eukaryota
ENOG410XR7R LUCA
GeneTreeiENSGT00490000043360
HOVERGENiHBG053834
InParanoidiQ80U16
OrthoDBiEOG091G018M
PhylomeDBiQ80U16
TreeFamiTF329332

Miscellaneous databases

ChiTaRSiFam65b mouse
PROiPR:Q80U16
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036006 Expressed in 249 organ(s), highest expression level in blood
CleanExiMM_6330500D04RIK
ExpressionAtlasiQ80U16 baseline and differential
GenevisibleiQ80U16 MM

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR031780 FAM65_N
IPR033035 FAM65B
IPR026136 RIPOR3
PANTHERiPTHR15829 PTHR15829, 1 hit
PTHR15829:SF2 PTHR15829:SF2, 1 hit
PfamiView protein in Pfam
PF15903 PL48, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRIPR2_MOUSE
AccessioniPrimary (citable) accession number: Q80U16
Secondary accession number(s): A6PW01
, A6PW03, Q3TQ58, Q5SY29, Q5SZV4, Q5T0J1, Q8BIM7, Q8BJ19, Q8BJ22, Q8BJ32, Q8BJ54
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: November 7, 2018
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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