UniProtKB - Q80U12 (SGSM2_MOUSE)
Protein
Small G protein signaling modulator 2
Gene
Sgsm2
Organism
Mus musculus (Mouse)
Status
Functioni
Possesses GTPase activator activity towards RAB32, RAB33B and RAB38. Regulates the trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes by inactivating RAB32 and RAB38. Inhibits RAB32 and RAB38 activation both directly by promoting their GTPase activity and indirectly by disrupting the RAB9A-HPS4 interaction which is required for RAB32/38 activation (PubMed:26620560).1 Publication
GO - Molecular functioni
- GTPase activator activity Source: UniProtKB
- Rab GTPase binding Source: MGI
GO - Biological processi
- activation of GTPase activity Source: GO_Central
- intracellular protein transport Source: GO_Central
- late endosome to Golgi transport Source: MGI
- positive regulation of GTPase activity Source: MGI
Keywordsi
Molecular function | GTPase activation |
Names & Taxonomyi
Protein namesi | Recommended name: Small G protein signaling modulator 2Alternative name(s): RUN and TBC1 domain-containing protein 1 |
Gene namesi | Name:Sgsm2 Synonyms:Kiaa0397, Rutbc1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2144695, Sgsm2 |
Subcellular locationi
Other locations
- Cytoplasm By similarity
- Melanosome 1 Publication
Note: Melanosomal localization is mediated by RAB9A.By similarity
Other locations
- cytoplasm Source: MGI
- melanosome Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000284663 | 1 – 1005 | Small G protein signaling modulator 2Add BLAST | 1005 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 402 | PhosphoserineCombined sources | 1 | ||
Isoform 2 (identifier: Q80U12-2) | |||||
Modified residuei | 444 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q80U12 |
PeptideAtlasi | Q80U12 |
PRIDEi | Q80U12 |
PTM databases
iPTMneti | Q80U12 |
PhosphoSitePlusi | Q80U12 |
Expressioni
Tissue specificityi
Widely expressed.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000038351, Expressed in brain and 226 other tissues |
Genevisiblei | Q80U12, MM |
Interactioni
Subunit structurei
Interacts with RAB4A, RAB11A, RAP1A, RAP1B, RAP2A and RAP2B. No interaction with RAB27A.
Interacts with RAB9A (By similarity).
By similarityGO - Molecular functioni
- Rab GTPase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 220852, 3 interactors |
DIPi | DIP-61071N |
IntActi | Q80U12, 2 interactors |
STRINGi | 10090.ENSMUSP00000050496 |
Miscellaneous databases
RNActi | Q80U12, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 34 – 191 | RUNPROSITE-ProRule annotationAdd BLAST | 158 | |
Domaini | 566 – 938 | Rab-GAP TBCPROSITE-ProRule annotationAdd BLAST | 373 |
Domaini
The Rab-GAP TBC domain possesses GTPase activator activity.By similarity
Sequence similaritiesi
Belongs to the RUTBC family.Curated
Phylogenomic databases
eggNOGi | KOG1648, Eukaryota |
GeneTreei | ENSGT00940000159315 |
HOGENOMi | CLU_006235_0_0_1 |
InParanoidi | Q80U12 |
OMAi | FLATHPI |
OrthoDBi | 171826at2759 |
PhylomeDBi | Q80U12 |
TreeFami | TF318216 |
Family and domain databases
CDDi | cd15784, PH_RUTBC, 1 hit |
Gene3Di | 1.20.58.900, 1 hit |
InterProi | View protein in InterPro IPR000195, Rab-GTPase-TBC_dom IPR035969, Rab-GTPase_TBC_sf IPR004012, Run_dom IPR037213, Run_dom_sf IPR037745, SGSM1/2 IPR021935, SGSM1/2_RBD |
Pfami | View protein in Pfam PF12068, PH_RBD, 1 hit PF00566, RabGAP-TBC, 1 hit PF02759, RUN, 1 hit |
SMARTi | View protein in SMART SM00593, RUN, 1 hit SM00164, TBC, 1 hit |
SUPFAMi | SSF140741, SSF140741, 1 hit SSF47923, SSF47923, 2 hits |
PROSITEi | View protein in PROSITE PS50826, RUN, 1 hit PS50086, TBC_RABGAP, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q80U12-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGSAEDAVKE KLLWNVKKEV KQIMEEAVTR KFVHEDSSHI IALCGAVEAC
60 70 80 90 100
LLHQLRRRAA GFLRSDKMAA LFTKVGKTCP VAEDICHKVQ ELQQQAEGRK
110 120 130 140 150
PSGGSQEALR KQGSTGGKAP ALSPQALKHI WVRTALMEKV LDRVVQYLAE
160 170 180 190 200
NCSKYYEKEA LLADPVFGPI LACLLVGPCA LEYTKLKTAD HYWTDPSADE
210 220 230 240 250
LVQRHRIRGP PNRQDSPAKR PALGIRKRHS SGSASEDRLA ACAREYVESL
260 270 280 290 300
HQNSRTRLLY GKNNVLVQPK EDMEAVPGYL SLHQSAENLT LKWTPNQLMN
310 320 330 340 350
GTLGDSELEK SVYWDYALVV PFSQIVCIHC HQQKSGGTLV LVSQDGIQRP
360 370 380 390 400
PLHFPQGGHL LSFLSCLENG LLPRGQLEPP LWTQQGKGKV FPKLRKRSSI
410 420 430 440 450
RSIDVEELGV GRATDYVFRI IYPGHRHEHN AGDMIEMQGF GPSLTAWHLE
460 470 480 490 500
PLCSQGSSCL SCSSSSSPYA TPSHCSCIPD RLPLRLLCES MKRQIVSRAF
510 520 530 540 550
YGWLAYCRHL STVRTHLSAL VHHNIIPPDR PPGASGGLTK DVWSKYQKDE
560 570 580 590 600
KNYKELELLR QVYYGGVEHE IRKDVWPFLL GHYKFGMSKK EMEQVDTAVA
610 620 630 640 650
ARYQQVLAEW KACEVVVRQR EREAHPATLT KFSSGSSIDS HVQRLVHRDS
660 670 680 690 700
TISNDVFISV DDLEPSGPQD LEDSKPKREQ EPGAGTPGIA AAEQQSVEFD
710 720 730 740 750
SPDSGLPSSR NYSVASGIQS SLDEAQSVGF EDDGAGEDGS EGPATAAHTF
760 770 780 790 800
PGPHDPGQET LAPASELEAG QELAAVCAAA YTIELLDTVA LNLHRIDKDV
810 820 830 840 850
QRCDRNYWYF TTSNLERLRD IMCSYVWEHL DMGYVQGMCD LLAPLLVILD
860 870 880 890 900
NDQLAYSCFS HLMKRMGQNF PSGGAMDSHF ANMRSLIQIL DSELFELMHQ
910 920 930 940 950
NGDYTHFYFC YRWFLLDFKR ELLYEDVFAV WEVIWAARRI SSEHFVLFIA
960 970 980 990 1000
LALVEAYREI IRDNNMDFTD IIKFFNERAE RHDAQEILRI ARDLVHKVQM
LIDNK
Sequence cautioni
The sequence AAH58414 differs from that shown. Reason: Frameshift.Curated
The sequence AAH60163 differs from that shown. Reason: Frameshift.Curated
The sequence BAC65555 differs from that shown. Reason: Erroneous initiation.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_024599 | 430 | N → ITINYHHLAASRAASVDDDE EEEDKLHAMLSMICSRNLTA PNPMKD in isoform 2. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK122273 mRNA Translation: BAC65555.1 Different initiation. AL604066 Genomic DNA No translation available. BC058414 mRNA Translation: AAH58414.1 Frameshift. BC060163 mRNA Translation: AAH60163.1 Frameshift. |
CCDSi | CCDS36224.1 [Q80U12-1] CCDS88189.1 [Q80U12-2] |
RefSeqi | NP_922934.2, NM_197943.2 [Q80U12-1] XP_006534644.1, XM_006534581.3 |
Genome annotation databases
Ensembli | ENSMUST00000057631; ENSMUSP00000050496; ENSMUSG00000038351 [Q80U12-1] ENSMUST00000081799; ENSMUSP00000080489; ENSMUSG00000038351 [Q80U12-2] |
GeneIDi | 97761 |
KEGGi | mmu:97761 |
UCSCi | uc007kck.1, mouse [Q80U12-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK122273 mRNA Translation: BAC65555.1 Different initiation. AL604066 Genomic DNA No translation available. BC058414 mRNA Translation: AAH58414.1 Frameshift. BC060163 mRNA Translation: AAH60163.1 Frameshift. |
CCDSi | CCDS36224.1 [Q80U12-1] CCDS88189.1 [Q80U12-2] |
RefSeqi | NP_922934.2, NM_197943.2 [Q80U12-1] XP_006534644.1, XM_006534581.3 |
3D structure databases
SMRi | Q80U12 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 220852, 3 interactors |
DIPi | DIP-61071N |
IntActi | Q80U12, 2 interactors |
STRINGi | 10090.ENSMUSP00000050496 |
PTM databases
iPTMneti | Q80U12 |
PhosphoSitePlusi | Q80U12 |
Proteomic databases
PaxDbi | Q80U12 |
PeptideAtlasi | Q80U12 |
PRIDEi | Q80U12 |
Protocols and materials databases
Antibodypediai | 10736, 80 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000057631; ENSMUSP00000050496; ENSMUSG00000038351 [Q80U12-1] ENSMUST00000081799; ENSMUSP00000080489; ENSMUSG00000038351 [Q80U12-2] |
GeneIDi | 97761 |
KEGGi | mmu:97761 |
UCSCi | uc007kck.1, mouse [Q80U12-1] |
Organism-specific databases
CTDi | 9905 |
MGIi | MGI:2144695, Sgsm2 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG1648, Eukaryota |
GeneTreei | ENSGT00940000159315 |
HOGENOMi | CLU_006235_0_0_1 |
InParanoidi | Q80U12 |
OMAi | FLATHPI |
OrthoDBi | 171826at2759 |
PhylomeDBi | Q80U12 |
TreeFami | TF318216 |
Miscellaneous databases
BioGRID-ORCSi | 97761, 2 hits in 17 CRISPR screens |
ChiTaRSi | Sgsm2, mouse |
PROi | PR:Q80U12 |
RNActi | Q80U12, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000038351, Expressed in brain and 226 other tissues |
Genevisiblei | Q80U12, MM |
Family and domain databases
CDDi | cd15784, PH_RUTBC, 1 hit |
Gene3Di | 1.20.58.900, 1 hit |
InterProi | View protein in InterPro IPR000195, Rab-GTPase-TBC_dom IPR035969, Rab-GTPase_TBC_sf IPR004012, Run_dom IPR037213, Run_dom_sf IPR037745, SGSM1/2 IPR021935, SGSM1/2_RBD |
Pfami | View protein in Pfam PF12068, PH_RBD, 1 hit PF00566, RabGAP-TBC, 1 hit PF02759, RUN, 1 hit |
SMARTi | View protein in SMART SM00593, RUN, 1 hit SM00164, TBC, 1 hit |
SUPFAMi | SSF140741, SSF140741, 1 hit SSF47923, SSF47923, 2 hits |
PROSITEi | View protein in PROSITE PS50826, RUN, 1 hit PS50086, TBC_RABGAP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SGSM2_MOUSE | |
Accessioni | Q80U12Primary (citable) accession number: Q80U12 Secondary accession number(s): Q5SWE0, Q5SWE1, Q6PAP8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 17, 2007 |
Last sequence update: | April 17, 2007 | |
Last modified: | December 2, 2020 | |
This is version 119 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families