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Protein

Putative tyrosine-protein phosphatase auxilin

Gene

Dnajc6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles. Plays a role in clathrin-mediated endocytosis in neurons.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei189Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • clathrin coat disassembly Source: MGI
  • regulation of clathrin-dependent endocytosis Source: MGI
  • synaptic vesicle uncoating Source: SynGO

Keywordsi

Molecular functionChaperone, Hydrolase, Protein phosphatase

Enzyme and pathway databases

ReactomeiR-MMU-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Putative tyrosine-protein phosphatase auxilinCurated (EC:3.1.3.48)
Alternative name(s):
DnaJ homolog subfamily C member 6Imported
Gene namesi
Name:Dnajc6Imported
Synonyms:Kiaa0473Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1919935 Dnajc6

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

Mice have a high rate of early postnatal mortality, although surviving pups have a normal life span despite decreased body weight. Knockout animals have impaired synaptic vesicle recycling, with an increased number of clathrin-coated vesicles, and impaired clathrin-mediated endocytosis of synaptic vesicles in neuronal culture. There is an up-regulation of Gak, but this does not fully compensate for the lack of the protein. The brains from mutant mice do not display alterations in substantia nigra morphology or dopamine transporter abundance or distribution, in agreement with the lack of gait or movement abnormalities in the mutant animals.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002445171 – 938Putative tyrosine-protein phosphatase auxilinAdd BLAST938

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei137PhosphoserineBy similarity1
Modified residuei478PhosphoserineCombined sources1
Modified residuei481PhosphoserineCombined sources1
Modified residuei595PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80TZ3
PeptideAtlasiQ80TZ3
PRIDEiQ80TZ3

PTM databases

iPTMnetiQ80TZ3
PhosphoSitePlusiQ80TZ3
SwissPalmiQ80TZ3

Expressioni

Gene expression databases

BgeeiENSMUSG00000028528 Expressed in 211 organ(s), highest expression level in cerebellum
CleanExiMM_DNAJC6
ExpressionAtlasiQ80TZ3 baseline and differential
GenevisibleiQ80TZ3 MM

Interactioni

Subunit structurei

Interacts with HSPA8/HSC70. Interacts with CLTC. Interacts with AP2A2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi215514, 2 interactors
IntActiQ80TZ3, 6 interactors
MINTiQ80TZ3
STRINGi10090.ENSMUSP00000102546

Structurei

3D structure databases

ProteinModelPortaliQ80TZ3
SMRiQ80TZ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati61 – 6414
Repeati65 – 6824
Repeati69 – 7234
Domaini80 – 247Phosphatase tensin-typePROSITE-ProRule annotationAdd BLAST168
Domaini253 – 391C2 tensin-typePROSITE-ProRule annotationAdd BLAST139
Domaini874 – 938JPROSITE-ProRule annotationAdd BLAST65

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni61 – 723 X 4 AA approximate tandem repeatsAdd BLAST12

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi434 – 442SH3-bindingSequence analysis9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi491 – 785Pro-richAdd BLAST295

Keywords - Domaini

Repeat, SH3-binding

Phylogenomic databases

eggNOGiKOG0431 Eukaryota
KOG2283 Eukaryota
ENOG41129H2 LUCA
GeneTreeiENSGT00760000119113
HOGENOMiHOG000034235
HOVERGENiHBG004322
InParanoidiQ80TZ3
KOiK09526
OMAiLCWQDQK
OrthoDBiEOG091G06HJ
PhylomeDBiQ80TZ3
TreeFamiTF105165

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR036869 J_dom_sf
IPR029021 Prot-tyrosine_phosphatase-like
IPR014020 Tensin_C2-dom
IPR029023 Tensin_phosphatase
PfamiView protein in Pfam
PF10409 PTEN_C2, 1 hit
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SM01326 PTEN_C2, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS51182 C2_TENSIN, 1 hit
PS50076 DNAJ_2, 1 hit
PS51181 PPASE_TENSIN, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q80TZ3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTNPKSGVAE SAGLACSRAA AGENRMKDSE NKGASSPDME PSYGGGLFDM
60 70 80 90 100
VKGGAGRLFS NLKDNLKDTL KDTSSRVIQS VSSYTKGDLD FTYVTSRIIV
110 120 130 140 150
MSFPVDSVDI GFRNQVDDIR SFLDSRHLDH YTVYNLSPKS YRTAKFHSRV
160 170 180 190 200
SECSWPIRQA PSLHNLFAVC RNMYNWLLQN PKNVCVVHCL DGRAASSILV
210 220 230 240 250
GAMFIFCNLY STPGPAVRLL YAKRPGIGLS PSHRRYLGYM CDLLADKPYR
260 270 280 290 300
PHFKPLTIKA ITVSPVPFFN KQRNGCRPYC DVLIGETKIY STCTDFERMK
310 320 330 340 350
EYRVQDGKIF IPLNITVQGD VIVSMYHLRS TIGSRLQAKV TNTQIFQLQF
360 370 380 390 400
HSGFIPLDTT VLKFTKPELD ACDVPEKYPQ LFQVTLDIEV QPQDKVIDLT
410 420 430 440 450
PPWEHYCTKD VNPSILFSSQ QEHQDTLALG GQAPADLPPD HPRNLGQGGF
460 470 480 490 500
FASLCWQDQK SEKSRCEEDH AALVNQESEQ SDDELLTLSS PHGNAEGDKP
510 520 530 540 550
HGAKKPGKKQ QEPAAPPPPE EVDLLGLEGS DVSTNFSSLA APPSNSELLS
560 570 580 590 600
DLFGGVGATG PAQAGQAGVE DVFHPSGPVS AQSTPRRTAT SASASPTLRV
610 620 630 640 650
GEGATFDPFG APAKPPGQDL LGSFLNTSSA SSDPFLQPTR SPSPTVHASS
660 670 680 690 700
TPAVNIQPDI AGGWDWHTKP GGFGMGSKSA ATSPTGSSHG TPTHQSKPQT
710 720 730 740 750
LDPFADLGTL GSSSFASKPT TPTGLGGGFP PLSSPQKASP QPMGGGWQQP
760 770 780 790 800
AGYNWQQTQS KPQSSMPHSS PQNRPNYNVS FSAMPAGQSE RGKGSTNLEG
810 820 830 840 850
KQKAADFEDL LSSQGFNAHK DKKGPRTIAE MRKEEMAKEM DPEKLKILEW
860 870 880 890 900
IEGKERNIRA LLSTMHTVLW AGETKWKPVG MADLVTPEQV KKVYRRAVLV
910 920 930
VHPDKATGQP YEQYAKMIFM ELNDAWSEFE NQGQKPLY
Length:938
Mass (Da):102,299
Last modified:June 27, 2006 - v2
Checksum:i3DFB7D9275BEF3F6
GO
Isoform 2 (identifier: Q80TZ3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.

Show »
Length:900
Mass (Da):98,521
Checksum:i9D35C6511ECD7BE5
GO
Isoform 3 (identifier: Q80TZ3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: MTNPKSGVAESAGLACSRAAAGENRMKDSENK → MSLLGSYRKK...PHRASTTDSS

Show »
Length:968
Mass (Da):105,409
Checksum:i83C4E04C24405E01
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1B0B8B1B0B8_MOUSE
Putative tyrosine-protein phosphata...
Dnajc6
194Annotation score:
B1B0B7B1B0B7_MOUSE
Putative tyrosine-protein phosphata...
Dnajc6
116Annotation score:

Sequence cautioni

The sequence AAH60734 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti442P → T in BAC65575 (PubMed:12693553).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0195821 – 38Missing in isoform 2. 2 PublicationsAdd BLAST38
Alternative sequenceiVSP_0195831 – 32MTNPK…DSENK → MSLLGSYRKKTSSDGYESLQ LVDSHGDSSARGAAAGTQRA TAGAVRSPARQPPHRASTTD SS in isoform 3. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122293 mRNA Translation: BAC65575.1
AK049935 mRNA Translation: BAC33992.1
AK147570 mRNA Translation: BAE28000.1
AK147637 mRNA Translation: BAE28039.1
AK163657 mRNA Translation: BAE37443.1
BX323551 Genomic DNA No translation available.
BC060734 mRNA Translation: AAH60734.1 Different initiation.
BC064460 mRNA Translation: AAH64460.1
CCDSiCCDS18395.1 [Q80TZ3-2]
CCDS51237.1 [Q80TZ3-1]
CCDS51238.1 [Q80TZ3-3]
RefSeqiNP_001158055.1, NM_001164583.1 [Q80TZ3-3]
NP_001158056.1, NM_001164584.1 [Q80TZ3-1]
NP_001158057.1, NM_001164585.1 [Q80TZ3-2]
NP_940804.1, NM_198412.2 [Q80TZ3-2]
XP_017175882.1, XM_017320393.1
UniGeneiMm.76494

Genome annotation databases

EnsembliENSMUST00000038207; ENSMUSP00000044251; ENSMUSG00000028528 [Q80TZ3-1]
ENSMUST00000094953; ENSMUSP00000092560; ENSMUSG00000028528 [Q80TZ3-2]
ENSMUST00000106929; ENSMUSP00000102542; ENSMUSG00000028528 [Q80TZ3-2]
ENSMUST00000106930; ENSMUSP00000102543; ENSMUSG00000028528 [Q80TZ3-2]
ENSMUST00000106933; ENSMUSP00000102546; ENSMUSG00000028528 [Q80TZ3-3]
GeneIDi72685
KEGGimmu:72685
UCSCiuc008tvq.2 mouse [Q80TZ3-1]
uc008tvt.1 mouse [Q80TZ3-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122293 mRNA Translation: BAC65575.1
AK049935 mRNA Translation: BAC33992.1
AK147570 mRNA Translation: BAE28000.1
AK147637 mRNA Translation: BAE28039.1
AK163657 mRNA Translation: BAE37443.1
BX323551 Genomic DNA No translation available.
BC060734 mRNA Translation: AAH60734.1 Different initiation.
BC064460 mRNA Translation: AAH64460.1
CCDSiCCDS18395.1 [Q80TZ3-2]
CCDS51237.1 [Q80TZ3-1]
CCDS51238.1 [Q80TZ3-3]
RefSeqiNP_001158055.1, NM_001164583.1 [Q80TZ3-3]
NP_001158056.1, NM_001164584.1 [Q80TZ3-1]
NP_001158057.1, NM_001164585.1 [Q80TZ3-2]
NP_940804.1, NM_198412.2 [Q80TZ3-2]
XP_017175882.1, XM_017320393.1
UniGeneiMm.76494

3D structure databases

ProteinModelPortaliQ80TZ3
SMRiQ80TZ3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215514, 2 interactors
IntActiQ80TZ3, 6 interactors
MINTiQ80TZ3
STRINGi10090.ENSMUSP00000102546

PTM databases

iPTMnetiQ80TZ3
PhosphoSitePlusiQ80TZ3
SwissPalmiQ80TZ3

Proteomic databases

PaxDbiQ80TZ3
PeptideAtlasiQ80TZ3
PRIDEiQ80TZ3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038207; ENSMUSP00000044251; ENSMUSG00000028528 [Q80TZ3-1]
ENSMUST00000094953; ENSMUSP00000092560; ENSMUSG00000028528 [Q80TZ3-2]
ENSMUST00000106929; ENSMUSP00000102542; ENSMUSG00000028528 [Q80TZ3-2]
ENSMUST00000106930; ENSMUSP00000102543; ENSMUSG00000028528 [Q80TZ3-2]
ENSMUST00000106933; ENSMUSP00000102546; ENSMUSG00000028528 [Q80TZ3-3]
GeneIDi72685
KEGGimmu:72685
UCSCiuc008tvq.2 mouse [Q80TZ3-1]
uc008tvt.1 mouse [Q80TZ3-3]

Organism-specific databases

CTDi9829
MGIiMGI:1919935 Dnajc6
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0431 Eukaryota
KOG2283 Eukaryota
ENOG41129H2 LUCA
GeneTreeiENSGT00760000119113
HOGENOMiHOG000034235
HOVERGENiHBG004322
InParanoidiQ80TZ3
KOiK09526
OMAiLCWQDQK
OrthoDBiEOG091G06HJ
PhylomeDBiQ80TZ3
TreeFamiTF105165

Enzyme and pathway databases

ReactomeiR-MMU-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

PROiPR:Q80TZ3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028528 Expressed in 211 organ(s), highest expression level in cerebellum
CleanExiMM_DNAJC6
ExpressionAtlasiQ80TZ3 baseline and differential
GenevisibleiQ80TZ3 MM

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR036869 J_dom_sf
IPR029021 Prot-tyrosine_phosphatase-like
IPR014020 Tensin_C2-dom
IPR029023 Tensin_phosphatase
PfamiView protein in Pfam
PF10409 PTEN_C2, 1 hit
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SM01326 PTEN_C2, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS51182 C2_TENSIN, 1 hit
PS50076 DNAJ_2, 1 hit
PS51181 PPASE_TENSIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAUXI_MOUSE
AccessioniPrimary (citable) accession number: Q80TZ3
Secondary accession number(s): B1B0B9, Q6P2K9, Q8C7L9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: October 10, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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