Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Slit homolog 1 protein

Gene

Slit1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.By similarity2 Publications

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • heparan sulfate proteoglycan binding Source: MGI
  • Roundabout binding Source: MGI
  • signaling receptor binding Source: MGI

GO - Biological processi

  • axon extension involved in axon guidance Source: MGI
  • axon guidance Source: MGI
  • axonogenesis Source: MGI
  • dorsal/ventral axon guidance Source: MGI
  • motor neuron axon guidance Source: MGI
  • negative chemotaxis Source: MGI
  • negative regulation of axon extension involved in axon guidance Source: MGI
  • negative regulation of synapse assembly Source: UniProtKB
  • neuron projection morphogenesis Source: MGI
  • nuclear migration Source: MGI
  • olfactory bulb development Source: MGI
  • retinal ganglion cell axon guidance Source: MGI
  • tangential migration from the subventricular zone to the olfactory bulb Source: MGI
  • telencephalon cell migration Source: MGI

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Slit homolog 1 protein
Short name:
Slit-1
Gene namesi
Name:Slit1
Synonyms:Kiaa0813
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1315203 Slit1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Mice show significant axon guidance errors in a variety of pathways, including corticofugal, callosal and thalamocortical tracts. Mice double-deficient in SLIT1 and SLIT2 show retinal axon guidance defects and a disorganized lateral olfactory tract (LOT).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000000772334 – 1531Slit homolog 1 proteinAdd BLAST1498

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi72N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi192N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi286 ↔ 295By similarity
Glycosylationi406N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi443 ↔ 466By similarity
Disulfide bondi445 ↔ 487By similarity
Disulfide bondi513 ↔ 519By similarity
Disulfide bondi517 ↔ 526By similarity
Glycosylationi571N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi630N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi675 ↔ 698By similarity
Disulfide bondi677 ↔ 719By similarity
Glycosylationi762N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi801N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi806N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi929 ↔ 940By similarity
Disulfide bondi934 ↔ 950By similarity
Disulfide bondi952 ↔ 961By similarity
Disulfide bondi968 ↔ 979By similarity
Disulfide bondi973 ↔ 991By similarity
Disulfide bondi993 ↔ 1002By similarity
Disulfide bondi1009 ↔ 1020By similarity
Disulfide bondi1014 ↔ 1029By similarity
Glycosylationi1026N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1031 ↔ 1040By similarity
Disulfide bondi1047 ↔ 1060By similarity
Disulfide bondi1054 ↔ 1069By similarity
Disulfide bondi1071 ↔ 1080By similarity
Glycosylationi1079N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1087 ↔ 1098By similarity
Disulfide bondi1092 ↔ 1107By similarity
Disulfide bondi1109 ↔ 1118By similarity
Disulfide bondi1128 ↔ 1139By similarity
Disulfide bondi1133 ↔ 1148By similarity
Disulfide bondi1150 ↔ 1159By similarity
Glycosylationi1186N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1256N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1303N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1310 ↔ 1336By similarity
Disulfide bondi1339 ↔ 1349By similarity
Disulfide bondi1344 ↔ 1359By similarity
Disulfide bondi1361 ↔ 1370By similarity
Disulfide bondi1378 ↔ 1388By similarity
Disulfide bondi1383 ↔ 1398By similarity
Disulfide bondi1400 ↔ 1409By similarity
Disulfide bondi1419 ↔ 1429By similarity
Disulfide bondi1424 ↔ 1439By similarity
Disulfide bondi1441 ↔ 1450By similarity
Disulfide bondi1456 ↔ 1495By similarity
Disulfide bondi1474 ↔ 1509By similarity
Disulfide bondi1485 ↔ 1525By similarity
Disulfide bondi1489 ↔ 1527By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ80TR4
PaxDbiQ80TR4
PRIDEiQ80TR4

PTM databases

iPTMnetiQ80TR4
PhosphoSitePlusiQ80TR4

Expressioni

Developmental stagei

During retinal development, is expressed at E12.5 in the dorsocentral region of the retina, and at E17.5 is only very weakly expressed. In the developing optic chiasm is expressed at E12.5 around the junction of the optic nerve and the brain, with strongest expression dorsal to the site at which the optic stalk joins the diencephalon, and also weakly in a subset of the CD44/SSEA neurons. In the more dorsal region of the developing optic chiasm, is expressed in some distance posterior to the axons. However, more ventrally, is expressed in a region directly adjacent to the path taken by the RGC axons. By E17.5 is not longer be detected at the junction of the brain and optic nerve and is only weakly expressed by the CD44/SSEA neurons. Outside the developing brain detected at between E8.5 and E9.5 in the primordiun of the branchial arches, between E9.5 and 10.5 in the posterior dermamyotome. By E11.5 the expression pattern along somite boundaries was most prominent caudally. Weak expression was also observed in the nasal pit at E11.5. From E13.5 to E17.5 expression was observed in the trigeminal ganglion, in the olfactory epithelium, and in the neural layer of the retina in the developing eye (with strongest expression in the inner nuclear layer).2 Publications

Gene expression databases

BgeeiENSMUSG00000025020
CleanExiMM_SLIT1
ExpressionAtlasiQ80TR4 baseline and differential
GenevisibleiQ80TR4 MM

Interactioni

Subunit structurei

Interacts with GREM1 (By similarity) and ROBO1.By similarity1 Publication

GO - Molecular functioni

  • heparan sulfate proteoglycan binding Source: MGI
  • Roundabout binding Source: MGI
  • signaling receptor binding Source: MGI

Protein-protein interaction databases

BioGridi203327, 1 interactor
STRINGi10090.ENSMUSP00000025993

Structurei

3D structure databases

ProteinModelPortaliQ80TR4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 61LRRNTAdd BLAST28
Repeati62 – 83LRR 1Add BLAST22
Repeati86 – 107LRR 2Add BLAST22
Repeati110 – 131LRR 3Add BLAST22
Repeati134 – 155LRR 4Add BLAST22
Repeati158 – 179LRR 5Add BLAST22
Repeati182 – 203LRR 6Add BLAST22
Domaini215 – 265LRRCT 1Add BLAST51
Domaini273 – 309LRRNT 23Add BLAST37
Repeati310 – 331LRR 7Add BLAST22
Repeati334 – 355LRR 8Add BLAST22
Repeati358 – 379LRR 9Add BLAST22
Repeati382 – 403LRR 10Add BLAST22
Repeati406 – 427LRR 11Add BLAST22
Domaini439 – 489LRRCT 2Add BLAST51
Domaini504 – 540LRRNT 3Add BLAST37
Repeati541 – 562LRR 12Add BLAST22
Repeati566 – 587LRR 13Add BLAST22
Repeati590 – 611LRR 14Add BLAST22
Repeati614 – 635LRR 15Add BLAST22
Repeati638 – 659LRR 16Add BLAST22
Domaini671 – 721LRRCT 3Add BLAST51
Domaini725 – 761LRRNT 4Add BLAST37
Repeati762 – 783LRR 17Add BLAST22
Repeati785 – 806LRR 18Add BLAST22
Repeati809 – 830LRR 19Add BLAST22
Repeati833 – 854LRR 20Add BLAST22
Domaini866 – 916LRRCT 4Add BLAST51
Domaini927 – 962EGF-like 1PROSITE-ProRule annotationAdd BLAST36
Domaini964 – 1003EGF-like 2PROSITE-ProRule annotationAdd BLAST40
Domaini1005 – 1041EGF-like 3PROSITE-ProRule annotationAdd BLAST37
Domaini1043 – 1081EGF-like 4PROSITE-ProRule annotationAdd BLAST39
Domaini1083 – 1119EGF-like 5PROSITE-ProRule annotationAdd BLAST37
Domaini1124 – 1160EGF-like 6PROSITE-ProRule annotationAdd BLAST37
Domaini1163 – 1336Laminin G-likePROSITE-ProRule annotationAdd BLAST174
Domaini1337 – 1371EGF-like 7PROSITE-ProRule annotationAdd BLAST35
Domaini1374 – 1410EGF-like 8PROSITE-ProRule annotationAdd BLAST37
Domaini1415 – 1451EGF-like 9PROSITE-ProRule annotationAdd BLAST37
Domaini1456 – 1531CTCKPROSITE-ProRule annotationAdd BLAST76

Keywords - Domaini

EGF-like domain, Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiKOG4237 Eukaryota
COG4886 LUCA
GeneTreeiENSGT00910000144018
HOGENOMiHOG000116120
HOVERGENiHBG057959
InParanoidiQ80TR4
KOiK06838
OMAiKCVHGKC
OrthoDBiEOG091G0MHP
PhylomeDBiQ80TR4
TreeFamiTF332887

Family and domain databases

Gene3Di3.80.10.10, 5 hits
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR000483 Cys-rich_flank_reg_C
IPR006207 Cys_knot_C
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR003645 Fol_N
IPR001791 Laminin_G
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR000372 LRRNT
PfamiView protein in Pfam
PF00008 EGF, 4 hits
PF12661 hEGF, 3 hits
PF02210 Laminin_G_2, 1 hit
PF13855 LRR_8, 6 hits
PF01463 LRRCT, 4 hits
PF01462 LRRNT, 4 hits
SMARTiView protein in SMART
SM00041 CT, 1 hit
SM00181 EGF, 9 hits
SM00179 EGF_CA, 7 hits
SM00274 FOLN, 3 hits
SM00282 LamG, 1 hit
SM00369 LRR_TYP, 18 hits
SM00082 LRRCT, 4 hits
SM00013 LRRNT, 4 hits
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS01185 CTCK_1, 1 hit
PS01225 CTCK_2, 1 hit
PS00022 EGF_1, 9 hits
PS01186 EGF_2, 8 hits
PS50026 EGF_3, 9 hits
PS01187 EGF_CA, 2 hits
PS50025 LAM_G_DOMAIN, 1 hit
PS51450 LRR, 21 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q80TR4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALTPQRGSS SGLSRPELWL LLWAAAWRLG ATACPALCTC TGTTVDCHGT
60 70 80 90 100
GLQAIPKNIP RNTERLELNG NNITRIHKND FAGLKQLRVL QLMENQIGAV
110 120 130 140 150
ERGAFDDMKE LERLRLNRNQ LQVLPELLFQ NNQALSRLDL SENFLQAVPR
160 170 180 190 200
KAFRGATDLK NLQLDKNRIS CIEEGAFRAL RGLEVLTLNN NNITTIPVSS
210 220 230 240 250
FNHMPKLRTF RLHSNHLFCD CHLAWLSQWL RQRPTIGLFT QCSGPASLRG
260 270 280 290 300
LNVAEVQKGE FSCSGQGEAA GAPACTLSSG SCPAMCSCSS GIVDCRGKGL
310 320 330 340 350
TAIPANLPET MTEIRLELNG IKSIPPGAFS PYRKLRRIDL SNNQIAEIAP
360 370 380 390 400
DAFQGLRSLN SLVLYGNKIT DLPRGVFGGL YTLQLLLLNA NKINCIRPDA
410 420 430 440 450
FQDLQNLSLL SLYDNKIQSL AKGTFTSLRA IQTLHLAQNP FICDCNLKWL
460 470 480 490 500
ADFLRTNPIE TTGARCASPR RLANKRIGQI KSKKFRCSAK EQYFIPGTED
510 520 530 540 550
YHLNSECTSD VACPHKCRCE ASVVECSSLK LSKIPERIPQ STTELRLNNN
560 570 580 590 600
EISILEATGL FKKLSHLKKI NLSNNKVSEI EDGTFEGAAS VSELHLTANQ
610 620 630 640 650
LESIRSGMFR GLDGLRTLML RNNRISCIHN DSFTGLRNVR LLSLYDNHIT
660 670 680 690 700
TISPGAFDTL QALSTLNLLA NPFNCNCHLS WLGDWLRKRK IVTGNPRCQN
710 720 730 740 750
PDFLRQIPLQ DVAFPDFRCE EGQEEVGCLP RPQCPQECAC LDTVVRCSNK
760 770 780 790 800
HLQALPKGIP KNVTELYLDG NQFTLVPGQL STFKYLQLVD LSNNKISSLS
810 820 830 840 850
NSSFTNMSQL TTLILSYNAL QCIPPLAFQG LRSLRLLSLH GNDVSTLQEG
860 870 880 890 900
IFADVTSLSH LAIGANPLYC DCRLRWLSSW VKTGYKEPGI ARCAGPPEME
910 920 930 940 950
GKLLLTTPAK KFECQGPPSL AVQAKCDPCL SSPCQNQGTC HNDPLEVYRC
960 970 980 990 1000
TCPSGYKGRH CEVSLDGCSS NPCGNGGTCH AQEGEDAGFT CSCPSGFEGP
1010 1020 1030 1040 1050
TCGVDTDDCV KHACVNGGVC VDGVGNYTCQ CPLQYTGRAC EQLVDFCSPD
1060 1070 1080 1090 1100
MNPCQHEAQC VGTPDGPRCE CMLGYTGDNC SENQDDCKDH KCQNGAQCVD
1110 1120 1130 1140 1150
EVNSYACLCV EGYSGQLCEI PPAPRSSCEG TECQNGANCV DQGSRPVCQC
1160 1170 1180 1190 1200
LPGFGGPECE KLLSVNFVDR DTYLQFTDLQ NWPRANITLQ VSTAEDNGIL
1210 1220 1230 1240 1250
LYNGDNDHIA VELYQGHVRV SYDPGSYPSS AIYSAETIND GQFHTVELVT
1260 1270 1280 1290 1300
FDQMVNLSID GGSPMTMDNF GKHYTLNSEA PLYVGGMPVD VNSAAFRLWQ
1310 1320 1330 1340 1350
ILNGTSFHGC IRNLYINNEL QDFTKTQMKP GVVPGCEPCR KLYCLHGICQ
1360 1370 1380 1390 1400
PNATPGPVCH CEAGWGGLHC DQPVDGPCHG HKCVHGKCVP LDALAYSCQC
1410 1420 1430 1440 1450
QDGYSGALCN QVGAVAEPCG GLQCLHGHCQ ASATKGAHCV CSPGFSGELC
1460 1470 1480 1490 1500
EQESECRGDP VRDFHRVQRG YAICQTTRPL SWVECRGACP GQGCCQGLRL
1510 1520 1530
KRRKLTFECS DGTSFAEEVE KPTKCGCAQC A
Length:1,531
Mass (Da):167,420
Last modified:March 15, 2004 - v2
Checksum:iC0F1C5A4E3DF6108
GO

Sequence cautioni

The sequence BAC65658 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44Missing in AAH57131 (PubMed:15489334).Curated1
Sequence conflicti44Missing in AAH62091 (PubMed:15489334).Curated1
Sequence conflicti830G → R in AAD44758 (PubMed:10433822).Curated1
Sequence conflicti1531A → V in AAD44758 (PubMed:10433822).Curated1
Sequence conflicti1531A → V in BAC65658 (PubMed:12693553).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF144627 mRNA Translation: AAD44758.1
AK122376 mRNA Translation: BAC65658.1 Different initiation.
BC057131 mRNA Translation: AAH57131.1
BC062091 mRNA Translation: AAH62091.1
CCDSiCCDS29812.1
RefSeqiNP_056563.2, NM_015748.3
XP_011245502.1, XM_011247200.2
UniGeneiMm.39155
Mm.40322

Genome annotation databases

EnsembliENSMUST00000025993; ENSMUSP00000025993; ENSMUSG00000025020
GeneIDi20562
KEGGimmu:20562
UCSCiuc008hmf.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSLIT1_MOUSE
AccessioniPrimary (citable) accession number: Q80TR4
Secondary accession number(s): Q9WVB5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 15, 2004
Last modified: June 20, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health