UniProtKB - Q80TG9 (LRFN2_MOUSE)
Protein
Leucine-rich repeat and fibronectin type-III domain-containing protein 2
Gene
Lrfn2
Organism
Mus musculus (Mouse)
Status
Functioni
Promotes neurite outgrowth in hippocampal neurons. Enhances the cell surface expression of 2 NMDA receptor subunits GRIN1 and GRIN2A (By similarity). May play a role in redistributing DLG4 to the cell periphery.By similarity1 Publication
GO - Biological processi
- modulation of chemical synaptic transmission Source: SynGO
- regulation of postsynapse organization Source: SynGO
Enzyme and pathway databases
Reactomei | R-MMU-8849932, Synaptic adhesion-like molecules |
Names & Taxonomyi
Protein namesi | Recommended name: Leucine-rich repeat and fibronectin type-III domain-containing protein 2 |
Gene namesi | Name:Lrfn2 Synonyms:Kiaa1246, Salm1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1917780, Lrfn2 |
Subcellular locationi
Plasma membrane
- postsynaptic cell membrane By similarity
Other locations
- Membrane 1 Publication; Single-pass type I membrane protein 1 Publication
- synapse 1 Publication
Plasma Membrane
Other locations
- cell surface Source: MGI
- Schaffer collateral - CA1 synapse Source: SynGO
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 21 – 534 | ExtracellularSequence analysisAdd BLAST | 514 | |
Transmembranei | 535 – 555 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 556 – 788 | CytoplasmicSequence analysisAdd BLAST | 233 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 787 – 788 | Missing : Loss of DLG4-binding. 1 Publication | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_0000014840 | 21 – 788 | Leucine-rich repeat and fibronectin type-III domain-containing protein 2Add BLAST | 768 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 29 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 310 ↔ 359 | PROSITE-ProRule annotation | ||
Glycosylationi | 332 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 341 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 384 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Glycosylated.1 Publication
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MaxQBi | Q80TG9 |
PaxDbi | Q80TG9 |
PRIDEi | Q80TG9 |
PTM databases
GlyGeni | Q80TG9, 4 sites |
iPTMneti | Q80TG9 |
PhosphoSitePlusi | Q80TG9 |
Expressioni
Tissue specificityi
Predominantly expressed in the brain, with a weak, but broad expression in the cerebral cortex and diencephalic nuclei. Strongly expressed in both the pyramidal layer and the dentate gyrus of the hippocampus. Also detected in other parts of the central nervous system, including the olfactory bulb, pons, cerebellum, and medulla oblongata, as well as in the peripheral nervous system, such as the ganglia of cranial nerves and the dorsal root ganglion during gestation.1 Publication
Developmental stagei
Expression starts around 11.5-12.5 dpc. At 11.5 dpc, detected in the outer layer of the telencephalic vesicles. This pattern of expression continues until 17.5 dpc with expression in the cortical plate, but not in the inner layer of the cerebral cortex, including subplate, ventricular zone, and subventricular zone. As also detected in the hippocampus, amygdala and widely in diencephalic nuclei.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000040490, Expressed in spinal cord and 87 other tissues |
Genevisiblei | Q80TG9, MM |
Interactioni
Subunit structurei
Forms heteromeric complexes with LRFN1, LRFN3, LRFN4 and LRFN5. Can form homomeric complexes, but not across cell junctions (By similarity).
Interacts with DLG4. Directly interacts with DLG1, DLG2 and DLG3 (By similarity). Directly interacts with 2 NMDA receptor subunits GRIN1 and GRIN2A (By similarity).
By similarityBinary interactionsi
Hide detailsQ80TG9
Protein-protein interaction databases
IntActi | Q80TG9, 6 interactors |
STRINGi | 10090.ENSMUSP00000047573 |
Miscellaneous databases
RNActi | Q80TG9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – 52 | LRRNTAdd BLAST | 32 | |
Repeati | 53 – 74 | LRR 1Add BLAST | 22 | |
Repeati | 77 – 98 | LRR 2Add BLAST | 22 | |
Repeati | 101 – 122 | LRR 3Add BLAST | 22 | |
Repeati | 125 – 146 | LRR 4Add BLAST | 22 | |
Repeati | 150 – 171 | LRR 5Add BLAST | 22 | |
Repeati | 174 – 195 | LRR 6Add BLAST | 22 | |
Repeati | 198 – 219 | LRR 7Add BLAST | 22 | |
Domaini | 242 – 288 | LRRCTAdd BLAST | 47 | |
Domaini | 289 – 375 | Ig-likeAdd BLAST | 87 | |
Domaini | 422 – 518 | Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST | 97 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 785 – 788 | PDZ-binding | 4 |
Domaini
The PDZ-binding motif is required for cell surface expression, neurite outgrowth promotion and interaction with DLG1, DLG3 and DLG4.By similarity
Sequence similaritiesi
Belongs to the LRFN family.Curated
Keywords - Domaini
Immunoglobulin domain, Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0619, Eukaryota |
GeneTreei | ENSGT00940000156417 |
HOGENOMi | CLU_016998_1_0_1 |
InParanoidi | Q80TG9 |
OMAi | HNIDRLM |
OrthoDBi | 151757at2759 |
PhylomeDBi | Q80TG9 |
TreeFami | TF350185 |
Family and domain databases
CDDi | cd00063, FN3, 1 hit |
Gene3Di | 2.60.40.10, 2 hits 3.80.10.10, 2 hits |
InterProi | View protein in InterPro IPR000483, Cys-rich_flank_reg_C IPR003961, FN3_dom IPR036116, FN3_sf IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf |
Pfami | View protein in Pfam PF00041, fn3, 1 hit PF07679, I-set, 1 hit PF13855, LRR_8, 2 hits |
SMARTi | View protein in SMART SM00409, IG, 1 hit SM00408, IGc2, 1 hit SM00369, LRR_TYP, 6 hits SM00082, LRRCT, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit SSF49265, SSF49265, 1 hit |
PROSITEi | View protein in PROSITE PS50853, FN3, 1 hit PS50835, IG_LIKE, 1 hit PS51450, LRR, 6 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q80TG9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
METLLGGLLA FGMAFAVVDA CPKYCVCQNL SESLGTLCPS KGLLFVPPDI
60 70 80 90 100
DRRTVELRLG GNFIIHIGRQ DFANMTGLVD LTLSRNTISH IQPFSFLDLE
110 120 130 140 150
SLRSLHLDSN RLPSLGEDTL RGLVNLQHLI VNNNQLGGIA DDAFEDFLLT
160 170 180 190 200
LEDLDLSYNN LHGLPWDSVR RMVNLHQLSL DHNLLDHIAE GTFADLQKLA
210 220 230 240 250
RLDLTSNRLQ KLPPDPIFAR SQASLLTATP FAPPLSFSFG GNPLHCNCEL
260 270 280 290 300
LWLRRLERDD DLETCGSPGS LKGRYFWHIR EEEFVCEPPL ITQHTHKLLV
310 320 330 340 350
LEGQAATLKC KAIGDPSPLI HWVAPDDRLV GNSSRTAVYD NGTLDILITT
360 370 380 390 400
SQDSGPFTCI AANAAGEATA TVEVSIVQLP HLSNSTSRMA PPKSRLSDIT
410 420 430 440 450
GSSKTSRGGG GSGAGEPPKS TPERAVLVSD VTTTSALVKW SVSKSAPRVK
460 470 480 490 500
MYQLQYNCSD DEVLIYRMIP ASNKAFVVNN LVSGTGYDLC VLAMWDDTAT
510 520 530 540 550
TLTATNIVGC AQFFTKADYP QCQSMHSQIL GGTMILVIGG IIVATLLVFI
560 570 580 590 600
VILMVRYKVC NHDTPGKMAA ATVSNVYSQT NGSQPPPLGG IPVGQLPQAP
610 620 630 640 650
PKVVVRNELM DFSTSLARAC DSSSSSSLGS GEAAGLGRGP WRLPPPAPRP
660 670 680 690 700
KPSLDRLMGA FASLDLKSQR KEELLDSRTP AGRGAGTSSR GHHSDREPLL
710 720 730 740 750
GPPATRARSL LPLPLEGKAK RSHSFDMGDF AAAAAAVPGG YSPPRRVSNI
760 770 780
WTKRSLSVNG MLLPFEESDL VGARGTFGSS EWVMESTV
Sequence cautioni
The sequence BAC65758 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 42 | G → R in BAB30828 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 530 | L → K in BAB30828 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK017594 mRNA Translation: BAB30828.1 AK044375 mRNA Translation: BAC31891.1 AK122476 mRNA Translation: BAC65758.1 Different initiation. CH466559 Genomic DNA Translation: EDL23629.1 BC139418 mRNA Translation: AAI39419.1 |
CCDSi | CCDS28871.1 |
RefSeqi | NP_081728.2, NM_027452.3 |
Genome annotation databases
Ensembli | ENSMUST00000046254; ENSMUSP00000047573; ENSMUSG00000040490 |
GeneIDi | 70530 |
KEGGi | mmu:70530 |
UCSCi | uc008cyd.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK017594 mRNA Translation: BAB30828.1 AK044375 mRNA Translation: BAC31891.1 AK122476 mRNA Translation: BAC65758.1 Different initiation. CH466559 Genomic DNA Translation: EDL23629.1 BC139418 mRNA Translation: AAI39419.1 |
CCDSi | CCDS28871.1 |
RefSeqi | NP_081728.2, NM_027452.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q80TG9, 6 interactors |
STRINGi | 10090.ENSMUSP00000047573 |
PTM databases
GlyGeni | Q80TG9, 4 sites |
iPTMneti | Q80TG9 |
PhosphoSitePlusi | Q80TG9 |
Proteomic databases
MaxQBi | Q80TG9 |
PaxDbi | Q80TG9 |
PRIDEi | Q80TG9 |
Protocols and materials databases
Antibodypediai | 2525, 107 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000046254; ENSMUSP00000047573; ENSMUSG00000040490 |
GeneIDi | 70530 |
KEGGi | mmu:70530 |
UCSCi | uc008cyd.2, mouse |
Organism-specific databases
CTDi | 57497 |
MGIi | MGI:1917780, Lrfn2 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG0619, Eukaryota |
GeneTreei | ENSGT00940000156417 |
HOGENOMi | CLU_016998_1_0_1 |
InParanoidi | Q80TG9 |
OMAi | HNIDRLM |
OrthoDBi | 151757at2759 |
PhylomeDBi | Q80TG9 |
TreeFami | TF350185 |
Enzyme and pathway databases
Reactomei | R-MMU-8849932, Synaptic adhesion-like molecules |
Miscellaneous databases
BioGRID-ORCSi | 70530, 2 hits in 18 CRISPR screens |
ChiTaRSi | Lrfn2, mouse |
PROi | PR:Q80TG9 |
RNActi | Q80TG9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000040490, Expressed in spinal cord and 87 other tissues |
Genevisiblei | Q80TG9, MM |
Family and domain databases
CDDi | cd00063, FN3, 1 hit |
Gene3Di | 2.60.40.10, 2 hits 3.80.10.10, 2 hits |
InterProi | View protein in InterPro IPR000483, Cys-rich_flank_reg_C IPR003961, FN3_dom IPR036116, FN3_sf IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf |
Pfami | View protein in Pfam PF00041, fn3, 1 hit PF07679, I-set, 1 hit PF13855, LRR_8, 2 hits |
SMARTi | View protein in SMART SM00409, IG, 1 hit SM00408, IGc2, 1 hit SM00369, LRR_TYP, 6 hits SM00082, LRRCT, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit SSF49265, SSF49265, 1 hit |
PROSITEi | View protein in PROSITE PS50853, FN3, 1 hit PS50835, IG_LIKE, 1 hit PS51450, LRR, 6 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LRFN2_MOUSE | |
Accessioni | Q80TG9Primary (citable) accession number: Q80TG9 Secondary accession number(s): B9EIF8, Q9CYK3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2005 |
Last sequence update: | March 29, 2005 | |
Last modified: | December 2, 2020 | |
This is version 148 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families