UniProtKB - Q80TE0 (RPAP1_MOUSE)
Protein
RNA polymerase II-associated protein 1
Gene
Rpap1
Organism
Mus musculus (Mouse)
Status
Functioni
Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. Required for interaction of the RNA polymerase II complex with acetylated histone H3 (By similarity).By similarity
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
GO - Biological processi
- transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Nucleotidyltransferase, Transferase |
Biological process | Transcription |
Names & Taxonomyi
Protein namesi | Recommended name: RNA polymerase II-associated protein 1 |
Gene namesi | Name:Rpap1 Synonyms:Kiaa1403 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1916175, Rpap1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- RNA polymerase complex Source: MGI
Keywords - Cellular componenti
DNA-directed RNA polymerase, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000284842 | 1 – 1409 | RNA polymerase II-associated protein 1Add BLAST | 1409 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 337 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q80TE0 |
PaxDbi | Q80TE0 |
PeptideAtlasi | Q80TE0 |
PRIDEi | Q80TE0 |
PTM databases
iPTMneti | Q80TE0 |
PhosphoSitePlusi | Q80TE0 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000034032, Expressed in ear vesicle and 303 other tissues |
ExpressionAtlasi | Q80TE0, baseline and differential |
Genevisiblei | Q80TE0, MM |
Interactioni
Subunit structurei
Part of an RNA polymerase II complex that contains POLR2A, POLR2B, POLR2C, POLR2D, POLR2E, POLR2F, POLR2G, POLR2H, POLR2I, POLR2J, POLR2K, POLR2L, RPAP1, FCP1 plus the general transcription factors TFIIB and TFIIF.
By similarityProtein-protein interaction databases
BioGRIDi | 213120, 10 interactors |
IntActi | Q80TE0, 1 interactor |
STRINGi | 10090.ENSMUSP00000097127 |
Miscellaneous databases
RNActi | Q80TE0, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 843 – 1302 | Leu-richAdd BLAST | 460 |
Sequence similaritiesi
Belongs to the RPAP1 family.Curated
Phylogenomic databases
eggNOGi | KOG1894, Eukaryota KOG4732, Eukaryota |
GeneTreei | ENSGT00390000007594 |
HOGENOMi | CLU_005296_1_0_1 |
InParanoidi | Q80TE0 |
OMAi | LRPRWCP |
OrthoDBi | 25908at2759 |
PhylomeDBi | Q80TE0 |
TreeFami | TF324391 |
Family and domain databases
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR013929, RNA_pol_II_AP1_C IPR013930, RNA_pol_II_AP1_N IPR039913, RPAP1/Rba50 |
PANTHERi | PTHR21483, PTHR21483, 1 hit |
Pfami | View protein in Pfam PF08620, RPAP1_C, 1 hit PF08621, RPAP1_N, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q80TE0-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MMLSRPKPGE SEVDLLRFQS QFLEAGAAPA VQLVKGSRRH GDAPPDRLPP
60 70 80 90 100
QDHRDVVMLD NLPDLPPALL PAPAKRARPS PGHPLPHDED PEERLNRHDQ
110 120 130 140 150
HITAVLSKIV ERDTSSVTVT LPVPSGVAFP PVFHRSQERQ VKPAASGKRS
160 170 180 190 200
IFAQEIAARR VSGNRVTSAE QVVPSLDTPE GAVPCETPSF RDRSNQLPGR
210 220 230 240 250
SHGFHRPNLV TGKGLRSKVA EQEVQTIHEE NVARLQAMDP EEILKEQQQL
260 270 280 290 300
LAQLDPSLVA FLRSHSQVQE QTGTKATKKQ SPKRPSVLVT KEEPVTSTRT
310 320 330 340 350
REPRTGDKLE EKPEATVEDK MEDKLQPRTP ALKLPMTPSK DWLHMDTVEL
360 370 380 390 400
DKLHWTQDLP PLRRQQTQER MQARFSLQGE LLAPDVDLPT HLGLHHHGEE
410 420 430 440 450
AERAGYSLQE LFHLTRSQVS QQRALALQVL SQIVGRAQAG EFGDRLVGSV
460 470 480 490 500
LRLLLDAGFL FLLRFSLDDR VDSVIAAAVR ALRTLLVAPG DEELLDRTFS
510 520 530 540 550
WYHGASVFPL MPSQDDKEDE DEDEELETEK VKRKTPEEGS RPPPDLARHD
560 570 580 590 600
VIKGLLATNL LPRLRYVLEV TCPGPSVILD ILAVLIRLAR HSLESAMRVL
610 620 630 640 650
ECPRLMETIV QEFLPTSWSP IGVGPTPSLY KVPCASAMKL LRVLASAGRN
660 670 680 690 700
IAARLLSGFD VRSRLCRFIA EAPHDLALPP EEAEILTTEA FRLWAVAASY
710 720 730 740 750
GQGGDLYREL YPVLLRALQT LPTELSAHPL QPLAMQRVAA LFTLLTQLTL
760 770 780 790 800
AASSIPPEPA SGPAESCVPA IPSSVTWTQV SGLKPLVEPC LKQTLKFLPR
810 820 830 840 850
PDVWNALGPV PSACLLFLGA YYQAWSRQSH LCPEDWLQDM ERLLDESLLP
860 870 880 890 900
LLSQPPLGSL WDSLRDCSPL CNPLSCASNP EALPSLVSLG CAGGCPPLSV
910 920 930 940 950
AGSASPFPFL TALLSLINTL VQSHKGLCGQ LSAVLTAPGL QNYFLQCVAP
960 970 980 990 1000
APAPQLTPFS AWALHHEYHL QYLVLSFAHK AATLQPEPAA STALHHAVAL
1010 1020 1030 1040 1050
VLLSRLLPGS EYLAQELLLS CVFRLEFLPE SASGGPEAAD FSDGLSLGSS
1060 1070 1080 1090 1100
GDPQCRRGAL LVQACRDLPS IRSCYLAHCS PARASLLSSQ ALYCGELLRV
1110 1120 1130 1140 1150
SSLLLPVPKE PLLATDWPFQ PLIHLYHRAS DTPSGPPAAD TVGVAMRVLQ
1160 1170 1180 1190 1200
WVLVLESWRP EVLWAVPPAA RLARLMCVYL VDSELFRETP IQRLVAALLA
1210 1220 1230 1240 1250
RLCRPQVLPN LKLDCPLPGL TSFPDLYASF LDHFEAVSFG DHLFGALVLL
1260 1270 1280 1290 1300
PLQRRFSVTL RLALFGEHVG VLRALGLPLT QLPVPLECYT EPAEDSLPLL
1310 1320 1330 1340 1350
QLYFRALVTG TLRARWCPIL YTVAVAHVNS FIFCQDPKSS DEVKTARRSM
1360 1370 1380 1390 1400
LQRTWLLTDE GLRQHLLHYK LPNSSLPEGF ELYSQLPRLR QQCLQTLPTE
GLQNGGVKT
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketV9GWX3 | V9GWX3_MOUSE | RNA polymerase II-associated protei... | Rpap1 | 869 | Annotation score: | ||
S4R2F2 | S4R2F2_MOUSE | RNA polymerase II-associated protei... | Rpap1 | 167 | Annotation score: |
Sequence cautioni
The sequence BAC65787 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 83 | H → R in BAC65787 (PubMed:12693553).Curated | 1 | |
Sequence conflicti | 83 | H → R in AAI17974 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 83 | H → R in AAI17975 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 126 | G → S in BAC65787 (PubMed:12693553).Curated | 1 | |
Sequence conflicti | 126 | G → S in AAI17975 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 190 | F → V in BAC65787 (PubMed:12693553).Curated | 1 | |
Sequence conflicti | 190 | F → V in AAI17974 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 190 | F → V in AAI17975 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 283 | K → E in BAC65787 (PubMed:12693553).Curated | 1 | |
Sequence conflicti | 283 | K → E in AAI17974 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 283 | K → E in AAI17975 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 432 | Q → R in AAI17974 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 527 | E → T in BAC65787 (PubMed:12693553).Curated | 1 | |
Sequence conflicti | 527 | E → T in AAI17974 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 527 | E → T in AAI17975 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 560 | L → Q in BAE39647 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 578 | I → V in BAC65787 (PubMed:12693553).Curated | 1 | |
Sequence conflicti | 578 | I → V in AAI17974 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 578 | I → V in AAI17975 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 879 | N → T in BAC65787 (PubMed:12693553).Curated | 1 | |
Sequence conflicti | 879 | N → T in BAE38647 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 879 | N → T in AAH51680 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 879 | N → T in AAI17974 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 880 | P → Q in BAE39647 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 942 | N → K in BAC65787 (PubMed:12693553).Curated | 1 | |
Sequence conflicti | 942 | N → K in AAH51680 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 942 | N → K in AAI17974 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 942 | N → K in AAI17975 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 977 | F → L in BAC65787 (PubMed:12693553).Curated | 1 | |
Sequence conflicti | 977 | F → L in AAH51680 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 977 | F → L in AAI17974 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 977 | F → L in AAI17975 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1066 | R → Q in BAC65787 (PubMed:12693553).Curated | 1 | |
Sequence conflicti | 1066 | R → Q in AAH51680 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1066 | R → Q in AAI17974 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1066 | R → Q in AAI17975 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1094 | C → R in BAC65787 (PubMed:12693553).Curated | 1 | |
Sequence conflicti | 1094 | C → R in AAH51680 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1094 | C → R in AAI17974 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1094 | C → R in AAI17975 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1183 | S → N in BAE39647 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 1263 | A → T in BAE38647 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 1305 | R → W in AAI17974 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1311 | T → S in BAC65787 (PubMed:12693553).Curated | 1 | |
Sequence conflicti | 1311 | T → S in AAH12218 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1311 | T → S in AAH51680 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1311 | T → S in AAI17974 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1311 | T → S in AAI17975 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_024682 | 1282 – 1290 | LPVPLECYT → VCPTLSRPS in isoform 2. 1 Publication | 9 | |
Alternative sequenceiVSP_024683 | 1291 – 1409 | Missing in isoform 2. 1 PublicationAdd BLAST | 119 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK122505 mRNA Translation: BAC65787.1 Different initiation. AK161487 mRNA Translation: BAE36419.1 AK166232 mRNA Translation: BAE38647.1 AK167589 mRNA Translation: BAE39647.1 AL844536 Genomic DNA No translation available. BC012218 mRNA Translation: AAH12218.1 BC051680 mRNA Translation: AAH51680.1 BC117973 mRNA Translation: AAI17974.1 BC117974 mRNA Translation: AAI17975.1 |
CCDSi | CCDS16610.1 [Q80TE0-1] |
RefSeqi | NP_001157173.1, NM_001163701.1 [Q80TE0-1] NP_796268.3, NM_177294.5 [Q80TE0-1] XP_006500184.1, XM_006500121.3 [Q80TE0-1] XP_011238061.1, XM_011239759.2 [Q80TE0-1] |
Genome annotation databases
Ensembli | ENSMUST00000048493; ENSMUSP00000037275; ENSMUSG00000034032 [Q80TE0-1] ENSMUST00000099529; ENSMUSP00000097127; ENSMUSG00000034032 [Q80TE0-1] ENSMUST00000110793; ENSMUSP00000106420; ENSMUSG00000034032 [Q80TE0-1] |
GeneIDi | 68925 |
KEGGi | mmu:68925 |
UCSCi | uc008luk.2, mouse [Q80TE0-1] uc008lun.2, mouse [Q80TE0-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK122505 mRNA Translation: BAC65787.1 Different initiation. AK161487 mRNA Translation: BAE36419.1 AK166232 mRNA Translation: BAE38647.1 AK167589 mRNA Translation: BAE39647.1 AL844536 Genomic DNA No translation available. BC012218 mRNA Translation: AAH12218.1 BC051680 mRNA Translation: AAH51680.1 BC117973 mRNA Translation: AAI17974.1 BC117974 mRNA Translation: AAI17975.1 |
CCDSi | CCDS16610.1 [Q80TE0-1] |
RefSeqi | NP_001157173.1, NM_001163701.1 [Q80TE0-1] NP_796268.3, NM_177294.5 [Q80TE0-1] XP_006500184.1, XM_006500121.3 [Q80TE0-1] XP_011238061.1, XM_011239759.2 [Q80TE0-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 213120, 10 interactors |
IntActi | Q80TE0, 1 interactor |
STRINGi | 10090.ENSMUSP00000097127 |
PTM databases
iPTMneti | Q80TE0 |
PhosphoSitePlusi | Q80TE0 |
Proteomic databases
EPDi | Q80TE0 |
PaxDbi | Q80TE0 |
PeptideAtlasi | Q80TE0 |
PRIDEi | Q80TE0 |
Protocols and materials databases
Antibodypediai | 42079, 118 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000048493; ENSMUSP00000037275; ENSMUSG00000034032 [Q80TE0-1] ENSMUST00000099529; ENSMUSP00000097127; ENSMUSG00000034032 [Q80TE0-1] ENSMUST00000110793; ENSMUSP00000106420; ENSMUSG00000034032 [Q80TE0-1] |
GeneIDi | 68925 |
KEGGi | mmu:68925 |
UCSCi | uc008luk.2, mouse [Q80TE0-1] uc008lun.2, mouse [Q80TE0-2] |
Organism-specific databases
CTDi | 26015 |
MGIi | MGI:1916175, Rpap1 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG1894, Eukaryota KOG4732, Eukaryota |
GeneTreei | ENSGT00390000007594 |
HOGENOMi | CLU_005296_1_0_1 |
InParanoidi | Q80TE0 |
OMAi | LRPRWCP |
OrthoDBi | 25908at2759 |
PhylomeDBi | Q80TE0 |
TreeFami | TF324391 |
Miscellaneous databases
BioGRID-ORCSi | 68925, 8 hits in 16 CRISPR screens |
ChiTaRSi | Rpap1, mouse |
PROi | PR:Q80TE0 |
RNActi | Q80TE0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000034032, Expressed in ear vesicle and 303 other tissues |
ExpressionAtlasi | Q80TE0, baseline and differential |
Genevisiblei | Q80TE0, MM |
Family and domain databases
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR013929, RNA_pol_II_AP1_C IPR013930, RNA_pol_II_AP1_N IPR039913, RPAP1/Rba50 |
PANTHERi | PTHR21483, PTHR21483, 1 hit |
Pfami | View protein in Pfam PF08620, RPAP1_C, 1 hit PF08621, RPAP1_N, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RPAP1_MOUSE | |
Accessioni | Q80TE0Primary (citable) accession number: Q80TE0 Secondary accession number(s): A2AQ24 Q91VM0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 17, 2007 |
Last sequence update: | April 17, 2007 | |
Last modified: | February 10, 2021 | |
This is version 117 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families