UniProtKB - Q80KJ8 (NS1A_MASV1)
Protein
Non-structural polyprotein 1A
Gene
ORF1
Organism
Mink astrovirus 1 (MAstV-1)
Status
Functioni
contains the viral protease participating in the cleavage of the polyprotein into functional products.
protein covalently attached to the 5' extremity of the genomic and subgenomic RNAs.By similarity
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 477 | Charge relay system; for serine protease activityBy similarity | 1 | |
Active sitei | 506 | Charge relay system; for serine protease activityBy similarity | 1 | |
Active sitei | 569 | Charge relay system; for serine protease activityBy similarity | 1 |
GO - Molecular functioni
- cysteine-type peptidase activity Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Viral RNA replication |
Names & Taxonomyi
Protein namesi | Recommended name: Non-structural polyprotein 1ACleaved into the following 5 chains: |
Gene namesi | Name:ORF1 |
Organismi | Mink astrovirus 1 (MAstV-1) |
Taxonomic identifieri | 1239574 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Stelpaviricetes › Stellavirales › Astroviridae › Mamastrovirus |
Virus hosti | Neovison vison (American mink) (Mustela vison) [TaxID: 452646] |
Proteomesi |
|
Subcellular locationi
- Host membrane Curated; Multi-pass membrane protein Curated
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 249 – 269 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 279 – 299 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 324 – 344 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 365 – 385 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Host membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000327345 | 1 – 874 | Non-structural polyprotein 1AAdd BLAST | 874 | |
ChainiPRO_0000327346 | 1 – 195 | Protein p19Sequence analysisAdd BLAST | 195 | |
ChainiPRO_0000327347 | 196 – 436 | Transmembrane protein 1ASequence analysisAdd BLAST | 241 | |
ChainiPRO_0000327348 | 437 – 662 | Serine protease p27Sequence analysisAdd BLAST | 226 | |
ChainiPRO_0000419599 | 666 – 752 | VPgSequence analysisAdd BLAST | 87 | |
ChainiPRO_0000327349 | 753 – 874 | Protein p20'Sequence analysisAdd BLAST | 122 |
Post-translational modificationi
Cleaved presumably by viral and host proteases.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 195 – 196 | CleavageSequence analysis | 2 | |
Sitei | 436 – 437 | CleavageSequence analysis | 2 | |
Sitei | 662 – 663 | CleavageSequence analysis | 2 | |
Sitei | 752 – 753 | CleavageSequence analysis | 2 |
Family & Domainsi
Sequence similaritiesi
Belongs to the astroviridae polyprotein 1A family.Curated
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixFamily and domain databases
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin |
SUPFAMi | SSF50494, SSF50494, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketIsoform nsp1a (identifier: Q80KJ8-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MANNTTSALH PRGSGQRCVY DTVLRFGDPD ARRRGFQLDE VSHNKLCDIF
60 70 80 90 100
DSGPLHFAFG DLKVMKVAGG VVTPHKTVVK TVYVSGVQEG NDYVTFAFTP
110 120 130 140 150
GPNEWREVDP RIDKRTALVG VLVQEHKKLD SDLKESRREL SQLKLEHSLL
160 170 180 190 200
RHDYERLVRE KPGPAMRTFK FSAVIFYAFF LGFLLMSAVK GEVYGRCLDS
210 220 230 240 250
ELNLNGNPEV CLHWEEVKSF SLQVALADFW NMTLDYYATV APQSPLMDLA
260 270 280 290 300
LGYFPYFANW HMAAFLVGTA HVVAAERPLY MLVTLVLATL SRFQLVALAA
310 320 330 340 350
VPMLDMPSSI GLWVTMVLFA IDQAFAILAS VLISVLLLIL CLAMNDVDYG
360 370 380 390 400
ALLRGCVTLV SATVFSHLVS FLHAPGWFTI IAILIYRIPK VLSYVSAERV
410 420 430 440 450
DIKGPDGKIK ETQNANPSWI TKMSGLKNFF QRAFRQKVRT GVNPTTRIIP
460 470 480 490 500
NSLVVIDAKD GRGTGFRVRN YLVTAGHVVG ADTTVRVRWA DVTSFAHVVY
510 520 530 540 550
RVPNKDIVLL TLPAEYNSLH SYKLAKEVVD GTVVVVSNGD GGALSVGISE
560 570 580 590 600
GVIVGESMTY AINTADGMSG SPLTTTDGRL IGVHQQNTGF TGGAVIFRDT
610 620 630 640 650
DFPQPKKPQR EADLEAKVAE LEKALAAYTQ SATGEDIVGL VRVAIQREME
660 670 680 690 700
VLRKELSNEF GQAKGKTKHK RRIMAAARSG GKRKPGKVWT EEEYKKLLEE
710 720 730 740 750
GFTRDQLREM AEAAREADDD FDDYEEEKNE VDYPVWSDHD SDEEIDRDWF
760 770 780 790 800
GQNLPTWSSA WSDFEPELDP DVTKTLPCHL EDKFSLKHYI ITEADLKHFG
810 820 830 840 850
QEMKEYMDHL DAVIKTHTEK GKWCPNTNTE EILKDLNAMW FKLNHTMWKN
860 870
GVAPFMQRKK QKPKNGKRAP KGAQ
Isoform nsp1ab (identifier: Q80KJ7-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q80KJ7.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY179509 Genomic RNA Translation: AAO32081.1 |
RefSeqi | NP_795334.1, NC_004579.1 [Q80KJ8-1] |
Genome annotation databases
GeneIDi | 1482922 |
KEGGi | vg:1482922 |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY179509 Genomic RNA Translation: AAO32081.1 |
RefSeqi | NP_795334.1, NC_004579.1 [Q80KJ8-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Genome annotation databases
GeneIDi | 1482922 |
KEGGi | vg:1482922 |
Family and domain databases
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin |
SUPFAMi | SSF50494, SSF50494, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NS1A_MASV1 | |
Accessioni | Q80KJ8Primary (citable) accession number: Q80KJ8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 8, 2008 |
Last sequence update: | June 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 94 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |