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Protein

E3 ubiquitin-protein ligase Siah1

Gene

siah1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It probably triggers the ubiquitin-mediated degradation of different substrates.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi98Zinc 1By similarity1
Metal bindingi105Zinc 1By similarity1
Metal bindingi117Zinc 1By similarity1
Metal bindingi121Zinc 1By similarity1
Metal bindingi128Zinc 2By similarity1
Metal bindingi135Zinc 2By similarity1
Metal bindingi147Zinc 2By similarity1
Metal bindingi152Zinc 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri41 – 76RING-typePROSITE-ProRule annotationAdd BLAST36
Zinc fingeri93 – 153SIAH-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Siah1 (EC:2.3.2.27)
Alternative name(s):
RING-type E3 ubiquitin transferase SIAH1Curated
Seven in absentia homolog 1
Short name:
Siah-1
Gene namesi
Name:siah1
ORF Names:zgc:56026
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-010319-31 siah1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000561671 – 282E3 ubiquitin-protein ligase Siah1Add BLAST282

Proteomic databases

PaxDbiQ7ZVG6

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000043842

Structurei

3D structure databases

ProteinModelPortaliQ7ZVG6
SMRiQ7ZVG6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni90 – 282SBDAdd BLAST193

Domaini

The RING-type zinc finger domain is essential for ubiquitin ligase activity.By similarity
The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family.By similarity

Sequence similaritiesi

Belongs to the SINA (Seven in absentia) family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri41 – 76RING-typePROSITE-ProRule annotationAdd BLAST36
Zinc fingeri93 – 153SIAH-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3002 Eukaryota
ENOG410XVP0 LUCA
HOVERGENiHBG055701
InParanoidiQ7ZVG6
KOiK04506
TreeFamiTF312976

Family and domain databases

Gene3Di2.60.210.10, 1 hit
3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR018121 7-in-absentia-prot_TRAF-dom
IPR004162 SINA-like
IPR008974 TRAF-like
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR013010 Znf_SIAH
PANTHERiPTHR10315 PTHR10315, 1 hit
PfamiView protein in Pfam
PF03145 Sina, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit
PS51081 ZF_SIAH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q7ZVG6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSRQTATALP TGTSKCPPSQ RVPTLSGTTA SNSDLASLFE CPVCFDYVLP
60 70 80 90 100
PILQCQSGHL VCSNCRPKLT CCPTCRGPLG SIRNLAMEKV ANSVLFPCKY
110 120 130 140 150
ASSGCEVTLP HTDKAEHEEL CEFRPYSCPC PGASCKWQGS LDAVMPHLLH
160 170 180 190 200
QHKSITTLQG EDIVFLATDI NLPGAVDWVM MQSCFGFHFM LVLEKQEKYD
210 220 230 240 250
GHQQFFAIVQ LIGTRKQAEN FAYRLELNGH RRRLTWEATP RSIHEGIATA
260 270 280
IMNSDCLVFD TSIAQLFAEN GNLGINVTIS MC
Length:282
Mass (Da):31,080
Last modified:April 26, 2004 - v2
Checksum:iDC879DF8A37F58E8
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0H2UKD5A0A0H2UKD5_DANRE
E3 ubiquitin-protein ligase
siah1
286Annotation score:
A0A0R4IPD0A0A0R4IPD0_DANRE
E3 ubiquitin-protein ligase
siah1
334Annotation score:

Sequence cautioni

The sequence AAH45870 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC045870 mRNA Translation: AAH45870.1 Different initiation.
RefSeqiNP_955815.1, NM_199521.2
UniGeneiDr.5064

Genome annotation databases

GeneIDi80955
KEGGidre:80955

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC045870 mRNA Translation: AAH45870.1 Different initiation.
RefSeqiNP_955815.1, NM_199521.2
UniGeneiDr.5064

3D structure databases

ProteinModelPortaliQ7ZVG6
SMRiQ7ZVG6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000043842

Proteomic databases

PaxDbiQ7ZVG6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi80955
KEGGidre:80955

Organism-specific databases

CTDi6477
ZFINiZDB-GENE-010319-31 siah1

Phylogenomic databases

eggNOGiKOG3002 Eukaryota
ENOG410XVP0 LUCA
HOVERGENiHBG055701
InParanoidiQ7ZVG6
KOiK04506
TreeFamiTF312976

Enzyme and pathway databases

UniPathwayi
UPA00143

Miscellaneous databases

PROiPR:Q7ZVG6

Family and domain databases

Gene3Di2.60.210.10, 1 hit
3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR018121 7-in-absentia-prot_TRAF-dom
IPR004162 SINA-like
IPR008974 TRAF-like
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR013010 Znf_SIAH
PANTHERiPTHR10315 PTHR10315, 1 hit
PfamiView protein in Pfam
PF03145 Sina, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit
PS51081 ZF_SIAH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSIAH1_DANRE
AccessioniPrimary (citable) accession number: Q7ZVG6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: November 7, 2018
This is version 101 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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