UniProtKB - Q7ZVF9 (ACTB2_DANRE)
Protein
Actin, cytoplasmic 2
Gene
actbb
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.By similarity
Miscellaneous
In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.By similarity
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- structural constituent of postsynaptic actin cytoskeleton Source: GO_Central
Keywordsi
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DRE-114608, Platelet degranulation R-DRE-190873, Gap junction degradation R-DRE-196025, Formation of annular gap junctions R-DRE-3928662, EPHB-mediated forward signaling R-DRE-3928665, EPH-ephrin mediated repulsion of cells R-DRE-418990, Adherens junctions interactions R-DRE-437239, Recycling pathway of L1 R-DRE-4420097, VEGFA-VEGFR2 Pathway R-DRE-445095, Interaction between L1 and Ankyrins R-DRE-446353, Cell-extracellular matrix interactions R-DRE-5250924, B-WICH complex positively regulates rRNA expression R-DRE-5626467, RHO GTPases activate IQGAPs R-DRE-5663220, RHO GTPases Activate Formins R-DRE-5674135, MAP2K and MAPK activation R-DRE-5689603, UCH proteinases R-DRE-5696394, DNA Damage Recognition in GG-NER R-DRE-8856828, Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: Actin, cytoplasmic 2Alternative name(s): Beta-actin-2 Cleaved into the following chain: |
Gene namesi | Name:actbb Synonyms:bactin2 |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-000329-3, actb2 |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
Nucleus
- Nucleus By similarity
Cytoskeleton
- actin filament Source: GO_Central
- cytoskeleton Source: AgBase
Nucleus
- nucleus Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: AgBase
Other locations
- dense body Source: AgBase
- focal adhesion Source: AgBase
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000367088 | 1 – 375 | Actin, cytoplasmic 2Add BLAST | 375 | |
Initiator methioninei | Removed; alternateBy similarity | |||
ChainiPRO_0000000797 | 2 – 375 | Actin, cytoplasmic 2, N-terminally processedAdd BLAST | 374 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionine; in Actin, cytoplasmic 2; alternateBy similarity | 1 | |
Modified residuei | 2 | N-acetylaspartate; in Actin, cytoplasmic 2, N-terminally processedBy similarity | 1 | |
Modified residuei | 44 | Methionine (R)-sulfoxideBy similarity | 1 | |
Modified residuei | 47 | Methionine (R)-sulfoxideBy similarity | 1 | |
Modified residuei | 73 | Tele-methylhistidine1 Publication | 1 |
Post-translational modificationi
Oxidation of Met-44 and Met-47 by MICALs (mical1, mical2 or mical3) to form methionine sulfoxide promotes actin filament depolymerization. Mical1 and mical2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by msrb1 and msrb2, which promote actin repolymerization (By similarity).By similarity
Methylation at His-73 by SETD3. Methylation stabilizes actin filaments.By similarity
Keywords - PTMi
Acetylation, Methylation, OxidationProteomic databases
PaxDbi | Q7ZVF9 |
PRIDEi | Q7ZVF9 |
Expressioni
Gene expression databases
Bgeei | ENSDARG00000037870, Expressed in granulocyte and 31 other tissues |
ExpressionAtlasi | Q7ZVF9, baseline and differential |
Interactioni
Subunit structurei
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix (By similarity). Each actin can bind to 4 others (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 7955.ENSDARP00000122263 |
Family & Domainsi
Sequence similaritiesi
Belongs to the actin family.Curated
Phylogenomic databases
eggNOGi | KOG0676, Eukaryota |
GeneTreei | ENSGT00950000182960 |
HOGENOMi | CLU_027965_0_2_1 |
InParanoidi | Q7ZVF9 |
OMAi | MPHAIAR |
OrthoDBi | 649708at2759 |
TreeFami | TF354237 |
Family and domain databases
InterProi | View protein in InterPro IPR004000, Actin IPR020902, Actin/actin-like_CS IPR004001, Actin_CS IPR043129, ATPase_NBD |
PANTHERi | PTHR11937, PTHR11937, 1 hit |
Pfami | View protein in Pfam PF00022, Actin, 1 hit |
PRINTSi | PR00190, ACTIN |
SMARTi | View protein in SMART SM00268, ACTIN, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
PROSITEi | View protein in PROSITE PS00406, ACTINS_1, 1 hit PS00432, ACTINS_2, 1 hit PS01132, ACTINS_ACT_LIKE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q7ZVF9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDDEIAALVV DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK
60 70 80 90 100
DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE
110 120 130 140 150
HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG
160 170 180 190 200
IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF
210 220 230 240 250
TTTAEREIVR DIKEKLCYVA LDFEQEMGTA ASSSSLEKSY ELPDGQVITI
260 270 280 290 300
GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS
310 320 330 340 350
GGTTMYPGIA DRMQKEITSL APSTMKIKII APPERKYSVW IGGSILASLS
360 370
TFQQMWISKQ EYDESGPSIV HRKCF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF1QC15 | F1QC15_DANRE | Actin, beta 2 | actb2 | 128 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 49 | Q → R in AAH67566 (Ref. 2) Curated | 1 | |
Sequence conflicti | 254 | R → G in AAH67566 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF025305 mRNA Translation: AAB97964.1 BC045879 mRNA Translation: AAH45879.1 BC067566 mRNA Translation: AAH67566.1 |
RefSeqi | NP_853632.3, NM_181601.4 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF025305 mRNA Translation: AAB97964.1 BC045879 mRNA Translation: AAH45879.1 BC067566 mRNA Translation: AAH67566.1 |
RefSeqi | NP_853632.3, NM_181601.4 |
3D structure databases
SMRi | Q7ZVF9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000122263 |
Proteomic databases
PaxDbi | Q7ZVF9 |
PRIDEi | Q7ZVF9 |
Genome annotation databases
Organism-specific databases
CTDi | 57935 |
ZFINi | ZDB-GENE-000329-3, actb2 |
Phylogenomic databases
eggNOGi | KOG0676, Eukaryota |
GeneTreei | ENSGT00950000182960 |
HOGENOMi | CLU_027965_0_2_1 |
InParanoidi | Q7ZVF9 |
OMAi | MPHAIAR |
OrthoDBi | 649708at2759 |
TreeFami | TF354237 |
Enzyme and pathway databases
Reactomei | R-DRE-114608, Platelet degranulation R-DRE-190873, Gap junction degradation R-DRE-196025, Formation of annular gap junctions R-DRE-3928662, EPHB-mediated forward signaling R-DRE-3928665, EPH-ephrin mediated repulsion of cells R-DRE-418990, Adherens junctions interactions R-DRE-437239, Recycling pathway of L1 R-DRE-4420097, VEGFA-VEGFR2 Pathway R-DRE-445095, Interaction between L1 and Ankyrins R-DRE-446353, Cell-extracellular matrix interactions R-DRE-5250924, B-WICH complex positively regulates rRNA expression R-DRE-5626467, RHO GTPases activate IQGAPs R-DRE-5663220, RHO GTPases Activate Formins R-DRE-5674135, MAP2K and MAPK activation R-DRE-5689603, UCH proteinases R-DRE-5696394, DNA Damage Recognition in GG-NER R-DRE-8856828, Clathrin-mediated endocytosis |
Miscellaneous databases
PROi | PR:Q7ZVF9 |
Gene expression databases
Bgeei | ENSDARG00000037870, Expressed in granulocyte and 31 other tissues |
ExpressionAtlasi | Q7ZVF9, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR004000, Actin IPR020902, Actin/actin-like_CS IPR004001, Actin_CS IPR043129, ATPase_NBD |
PANTHERi | PTHR11937, PTHR11937, 1 hit |
Pfami | View protein in Pfam PF00022, Actin, 1 hit |
PRINTSi | PR00190, ACTIN |
SMARTi | View protein in SMART SM00268, ACTIN, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
PROSITEi | View protein in PROSITE PS00406, ACTINS_1, 1 hit PS00432, ACTINS_2, 1 hit PS01132, ACTINS_ACT_LIKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACTB2_DANRE | |
Accessioni | Q7ZVF9Primary (citable) accession number: Q7ZVF9 Secondary accession number(s): O73815, P12714, Q6NWJ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 16, 2004 |
Last sequence update: | March 7, 2006 | |
Last modified: | December 2, 2020 | |
This is version 140 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families