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Protein

Transmembrane emp24 domain-containing protein 4

Gene

TMED4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in vesicular protein trafficking, mainly in the early secretory pathway. targeting. Involved in the maintenance of the Golgi apparatus. Appears to play a role in the biosynthesis of secreted cargo including processing. Involved in endoplasmic reticulum stress response. May play a role in the regulation of heat-shock response and apoptosis (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane emp24 domain-containing protein 4
Alternative name(s):
Endoplasmic reticulum stress-response protein 25
Short name:
ERS25
GMP25iso
Putative NF-kappa-B-activating protein 156
p24 family protein alpha-3
Short name:
p24alpha3
Gene namesi
Name:TMED4
Synonyms:ERS25
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000158604.14
HGNCiHGNC:22301 TMED4
MIMi612038 gene
neXtProtiNX_Q7Z7H5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 194LumenalSequence analysisAdd BLAST165
Transmembranei195 – 212HelicalSequence analysisAdd BLAST18
Topological domaini213 – 227CytoplasmicSequence analysisAdd BLAST15

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi222068
OpenTargetsiENSG00000158604
PharmGKBiPA134983854

Polymorphism and mutation databases

BioMutaiTMED4
DMDMi62287892

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000001038730 – 227Transmembrane emp24 domain-containing protein 4Add BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi117N-linked (GlcNAc...) asparagine1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ7Z7H5
MaxQBiQ7Z7H5
PaxDbiQ7Z7H5
PeptideAtlasiQ7Z7H5
PRIDEiQ7Z7H5
ProteomicsDBi69548
69549 [Q7Z7H5-2]
69550 [Q7Z7H5-3]

PTM databases

GlyConnecti1844
iPTMnetiQ7Z7H5
PhosphoSitePlusiQ7Z7H5
SwissPalmiQ7Z7H5

Expressioni

Gene expression databases

BgeeiENSG00000158604 Expressed in 213 organ(s), highest expression level in oviduct epithelium
CleanExiHS_TMED4
ExpressionAtlasiQ7Z7H5 baseline and differential
GenevisibleiQ7Z7H5 HS

Organism-specific databases

HPAiHPA014650

Interactioni

Protein-protein interaction databases

BioGridi128780, 39 interactors
IntActiQ7Z7H5, 7 interactors
STRINGi9606.ENSP00000404042

Structurei

3D structure databases

ProteinModelPortaliQ7Z7H5
SMRiQ7Z7H5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 137GOLDPROSITE-ProRule annotationAdd BLAST99

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili147 – 176Sequence analysisAdd BLAST30

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi220 – 227COPI vesicle coat-bindingSequence analysis8
Motifi220 – 221COPII vesicle coat-bindingSequence analysis2

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1690 Eukaryota
ENOG410XPUW LUCA
GeneTreeiENSGT00430000030848
HOGENOMiHOG000160228
HOVERGENiHBG105357
InParanoidiQ7Z7H5
KOiK20346
OMAiIRREQQY
OrthoDBiEOG091G0JDW
PhylomeDBiQ7Z7H5
TreeFamiTF314123

Family and domain databases

InterProiView protein in InterPro
IPR009038 GOLD_dom
IPR015720 TMP21-related
PANTHERiPTHR22811 PTHR22811, 1 hit
PfamiView protein in Pfam
PF01105 EMP24_GP25L, 1 hit
SMARTiView protein in SMART
SM01190 EMP24_GP25L, 1 hit
PROSITEiView protein in PROSITE
PS50866 GOLD, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q7Z7H5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAGVGAGPLR AMGRQALLLL ALCATGAQGL YFHIGETEKR CFIEEIPDET
60 70 80 90 100
MVIGNYRTQM WDKQKEVFLP STPGLGMHVE VKDPDGKVVL SRQYGSEGRF
110 120 130 140 150
TFTSHTPGDH QICLHSNSTR MALFAGGKLR VHLDIQVGEH ANNYPEIAAK
160 170 180 190 200
DKLTELQLRA RQLLDQVEQI QKEQDYQRYR EERFRLTSES TNQRVLWWSI
210 220
AQTVILILTG IWQMRHLKSF FEAKKLV
Length:227
Mass (Da):25,943
Last modified:October 1, 2003 - v1
Checksum:i3C650402F472A051
GO
Isoform 2 (identifier: Q7Z7H5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     59-74: Missing.

Note: No experimental confirmation available.
Show »
Length:211
Mass (Da):24,070
Checksum:iD8111386449A8A53
GO
Isoform 3 (identifier: Q7Z7H5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     179-186: YREERFRL → ASAYLLVI
     187-227: Missing.

Show »
Length:186
Mass (Da):20,781
Checksum:iDCEE9F5107DC4347
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8W7F7F8W7F7_HUMAN
Transmembrane emp24 domain-containi...
TMED4
178Annotation score:

Sequence cautioni

The sequence AAP20105 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC77362 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03569859 – 74Missing in isoform 2. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_035699179 – 186YREERFRL → ASAYLLVI in isoform 3. 2 Publications8
Alternative sequenceiVSP_035700187 – 227Missing in isoform 3. 2 PublicationsAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK074557 mRNA Translation: BAC11058.1
AK290125 mRNA Translation: BAF82814.1
AK294122 mRNA Translation: BAG57455.1
BX647965 mRNA No translation available.
CH236960 Genomic DNA Translation: EAL23751.1
CH471128 Genomic DNA Translation: EAW61090.1
CH471128 Genomic DNA Translation: EAW61091.1
BC052641 mRNA Translation: AAH52641.1
BC057851 mRNA Translation: AAH57851.1
AY191223 mRNA Translation: AAP20105.1 Different initiation.
AB097009 mRNA Translation: BAC77362.1 Different initiation.
CCDSiCCDS5493.1 [Q7Z7H5-1]
CCDS78227.1 [Q7Z7H5-3]
RefSeqiNP_001289987.1, NM_001303058.1 [Q7Z7H5-3]
NP_001289989.1, NM_001303060.1
NP_872353.2, NM_182547.3 [Q7Z7H5-1]
UniGeneiHs.598832

Genome annotation databases

EnsembliENST00000457408; ENSP00000404042; ENSG00000158604 [Q7Z7H5-1]
ENST00000481238; ENSP00000417443; ENSG00000158604 [Q7Z7H5-3]
GeneIDi222068
KEGGihsa:222068
UCSCiuc003tli.4 human [Q7Z7H5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK074557 mRNA Translation: BAC11058.1
AK290125 mRNA Translation: BAF82814.1
AK294122 mRNA Translation: BAG57455.1
BX647965 mRNA No translation available.
CH236960 Genomic DNA Translation: EAL23751.1
CH471128 Genomic DNA Translation: EAW61090.1
CH471128 Genomic DNA Translation: EAW61091.1
BC052641 mRNA Translation: AAH52641.1
BC057851 mRNA Translation: AAH57851.1
AY191223 mRNA Translation: AAP20105.1 Different initiation.
AB097009 mRNA Translation: BAC77362.1 Different initiation.
CCDSiCCDS5493.1 [Q7Z7H5-1]
CCDS78227.1 [Q7Z7H5-3]
RefSeqiNP_001289987.1, NM_001303058.1 [Q7Z7H5-3]
NP_001289989.1, NM_001303060.1
NP_872353.2, NM_182547.3 [Q7Z7H5-1]
UniGeneiHs.598832

3D structure databases

ProteinModelPortaliQ7Z7H5
SMRiQ7Z7H5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128780, 39 interactors
IntActiQ7Z7H5, 7 interactors
STRINGi9606.ENSP00000404042

PTM databases

GlyConnecti1844
iPTMnetiQ7Z7H5
PhosphoSitePlusiQ7Z7H5
SwissPalmiQ7Z7H5

Polymorphism and mutation databases

BioMutaiTMED4
DMDMi62287892

Proteomic databases

EPDiQ7Z7H5
MaxQBiQ7Z7H5
PaxDbiQ7Z7H5
PeptideAtlasiQ7Z7H5
PRIDEiQ7Z7H5
ProteomicsDBi69548
69549 [Q7Z7H5-2]
69550 [Q7Z7H5-3]

Protocols and materials databases

DNASUi222068
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000457408; ENSP00000404042; ENSG00000158604 [Q7Z7H5-1]
ENST00000481238; ENSP00000417443; ENSG00000158604 [Q7Z7H5-3]
GeneIDi222068
KEGGihsa:222068
UCSCiuc003tli.4 human [Q7Z7H5-1]

Organism-specific databases

CTDi222068
DisGeNETi222068
EuPathDBiHostDB:ENSG00000158604.14
GeneCardsiTMED4
HGNCiHGNC:22301 TMED4
HPAiHPA014650
MIMi612038 gene
neXtProtiNX_Q7Z7H5
OpenTargetsiENSG00000158604
PharmGKBiPA134983854
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1690 Eukaryota
ENOG410XPUW LUCA
GeneTreeiENSGT00430000030848
HOGENOMiHOG000160228
HOVERGENiHBG105357
InParanoidiQ7Z7H5
KOiK20346
OMAiIRREQQY
OrthoDBiEOG091G0JDW
PhylomeDBiQ7Z7H5
TreeFamiTF314123

Miscellaneous databases

ChiTaRSiTMED4 human
GeneWikiiTMED4
GenomeRNAii222068
PROiPR:Q7Z7H5
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000158604 Expressed in 213 organ(s), highest expression level in oviduct epithelium
CleanExiHS_TMED4
ExpressionAtlasiQ7Z7H5 baseline and differential
GenevisibleiQ7Z7H5 HS

Family and domain databases

InterProiView protein in InterPro
IPR009038 GOLD_dom
IPR015720 TMP21-related
PANTHERiPTHR22811 PTHR22811, 1 hit
PfamiView protein in Pfam
PF01105 EMP24_GP25L, 1 hit
SMARTiView protein in SMART
SM01190 EMP24_GP25L, 1 hit
PROSITEiView protein in PROSITE
PS50866 GOLD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTMED4_HUMAN
AccessioniPrimary (citable) accession number: Q7Z7H5
Secondary accession number(s): A4D2K8
, B4DFJ4, Q56VW3, Q7Z432, Q8N2P6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: October 1, 2003
Last modified: November 7, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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