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Protein

Ubiquitin-conjugating enzyme E2 Q1

Gene

UBE2Q1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins (PubMed:22496338). May be involved in hormonal homeostasis in females. Involved in regulation of B4GALT1 cell surface expression, B4GALT1-mediated cell adhesion to laminin and embryoid body formation (By similarity).By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei351Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiQ7Z7E8
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 Q1 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme Q1
Protein NICE-5
Ubiquitin carrier protein Q1
Ubiquitin-protein ligase Q1
Gene namesi
Name:UBE2Q1
Synonyms:NICE5, UBE2Q
ORF Names:PRO3094
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000160714.9
HGNCiHGNC:15698 UBE2Q1
MIMi617429 gene
neXtProtiNX_Q7Z7E8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000160714
PharmGKBiPA38029

Polymorphism and mutation databases

BioMutaiUBE2Q1
DMDMi74750234

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002238761 – 422Ubiquitin-conjugating enzyme E2 Q1Add BLAST422

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Post-translational modificationi

Autoubiquitinated in vitro in the presence of NEDD4L.1 Publication

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

EPDiQ7Z7E8
MaxQBiQ7Z7E8
PaxDbiQ7Z7E8
PeptideAtlasiQ7Z7E8
PRIDEiQ7Z7E8
ProteomicsDBi69523
69524 [Q7Z7E8-2]

PTM databases

iPTMnetiQ7Z7E8
PhosphoSitePlusiQ7Z7E8

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000160714 Expressed in 228 organ(s), highest expression level in testis
CleanExiHS_UBE2Q1
GenevisibleiQ7Z7E8 HS

Organism-specific databases

HPAiHPA063368

Interactioni

Subunit structurei

Monomer and homodimer. Only the homodimer is linked to ubiquitin through thiolester activation. Interacts (via N-terminus) with B4GALT1 (via N-terminal cytoplasmic domain). The interaction is direct.By similarity

Binary interactionsi

Protein-protein interaction databases

BioGridi120732, 24 interactors
IntActiQ7Z7E8, 8 interactors
MINTiQ7Z7E8
STRINGi9606.ENSP00000292211

Structurei

Secondary structure

1422
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ7Z7E8
SMRiQ7Z7E8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7Z7E8

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi8 – 38Gly-richAdd BLAST31

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0897 Eukaryota
ENOG410XRET LUCA
GeneTreeiENSGT00390000018573
HOVERGENiHBG061368
InParanoidiQ7Z7E8
KOiK10582
OMAiDSPLHND
OrthoDBiEOG091G0LGG
PhylomeDBiQ7Z7E8
TreeFamiTF313338

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 2 hits
PROSITEiView protein in PROSITE
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q7Z7E8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MQQPQPQGQQ QPGPGQQLGG QGAAPGAGGG PGGGPGPGPC LRRELKLLES
60 70 80 90 100
IFHRGHERFR IASACLDELS CEFLLAGAGG AGAGAAPGPH LPPRGSVPGD
110 120 130 140 150
PVRIHCNITE SYPAVPPIWS VESDDPNLAA VLERLVDIKK GNTLLLQHLK
160 170 180 190 200
RIISDLCKLY NLPQHPDVEM LDQPLPAEQC TQEDVSSEDE DEEMPEDTED
210 220 230 240 250
LDHYEMKEEE PAEGKKSEDD GIGKENLAIL EKIKKNQRQD YLNGAVSGSV
260 270 280 290 300
QATDRLMKEL RDIYRSQSFK GGNYAVELVN DSLYDWNVKL LKVDQDSALH
310 320 330 340 350
NDLQILKEKE GADFILLNFS FKDNFPFDPP FVRVVSPVLS GGYVLGGGAI
360 370 380 390 400
CMELLTKQGW SSAYSIESVI MQISATLVKG KARVQFGANK SQYSLTRAQQ
410 420
SYKSLVQIHE KNGWYTPPKE DG
Length:422
Mass (Da):46,127
Last modified:October 1, 2003 - v1
Checksum:i106FF7E59DF65555
GO
Isoform 2 (identifier: Q7Z7E8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-169: Missing.

Show »
Length:253
Mass (Da):28,473
Checksum:i63C896449FD35EF2
GO

Sequence cautioni

The sequence AAF71141 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH61583 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH87836 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAI07058 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB71173 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAB65097 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti287N → Y in CAB65097 (PubMed:11230159).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0172961 – 169Missing in isoform 2. 2 PublicationsAdd BLAST169

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY112698 mRNA Translation: AAM60814.1
AY948200 mRNA Translation: AAY23342.1
AK056388 mRNA Translation: BAB71173.1 Different initiation.
AK293986 mRNA Translation: BAG57353.1
AL592078 Genomic DNA No translation available.
CH471121 Genomic DNA Translation: EAW53193.1
BC000848 mRNA Translation: AAH00848.1
BC015316 mRNA Translation: AAH15316.2
BC061583 mRNA Translation: AAH61583.1 Different initiation.
BC070158 mRNA Translation: AAH70158.1
BC087836 mRNA Translation: AAH87836.1 Different initiation.
BC107057 mRNA Translation: AAI07058.1 Different initiation.
AJ243666 mRNA Translation: CAB65097.1 Different initiation.
AF116721 mRNA Translation: AAF71141.1 Different initiation.
CR457219 mRNA Translation: CAG33500.1
CCDSiCCDS1069.1 [Q7Z7E8-1]
RefSeqiNP_060052.3, NM_017582.6 [Q7Z7E8-1]
UniGeneiHs.607928

Genome annotation databases

EnsembliENST00000292211; ENSP00000292211; ENSG00000160714 [Q7Z7E8-1]
GeneIDi55585
KEGGihsa:55585
UCSCiuc001fff.2 human [Q7Z7E8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY112698 mRNA Translation: AAM60814.1
AY948200 mRNA Translation: AAY23342.1
AK056388 mRNA Translation: BAB71173.1 Different initiation.
AK293986 mRNA Translation: BAG57353.1
AL592078 Genomic DNA No translation available.
CH471121 Genomic DNA Translation: EAW53193.1
BC000848 mRNA Translation: AAH00848.1
BC015316 mRNA Translation: AAH15316.2
BC061583 mRNA Translation: AAH61583.1 Different initiation.
BC070158 mRNA Translation: AAH70158.1
BC087836 mRNA Translation: AAH87836.1 Different initiation.
BC107057 mRNA Translation: AAI07058.1 Different initiation.
AJ243666 mRNA Translation: CAB65097.1 Different initiation.
AF116721 mRNA Translation: AAF71141.1 Different initiation.
CR457219 mRNA Translation: CAG33500.1
CCDSiCCDS1069.1 [Q7Z7E8-1]
RefSeqiNP_060052.3, NM_017582.6 [Q7Z7E8-1]
UniGeneiHs.607928

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QGXX-ray2.56A/B/C/D247-414[»]
ProteinModelPortaliQ7Z7E8
SMRiQ7Z7E8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120732, 24 interactors
IntActiQ7Z7E8, 8 interactors
MINTiQ7Z7E8
STRINGi9606.ENSP00000292211

PTM databases

iPTMnetiQ7Z7E8
PhosphoSitePlusiQ7Z7E8

Polymorphism and mutation databases

BioMutaiUBE2Q1
DMDMi74750234

Proteomic databases

EPDiQ7Z7E8
MaxQBiQ7Z7E8
PaxDbiQ7Z7E8
PeptideAtlasiQ7Z7E8
PRIDEiQ7Z7E8
ProteomicsDBi69523
69524 [Q7Z7E8-2]

Protocols and materials databases

DNASUi55585
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000292211; ENSP00000292211; ENSG00000160714 [Q7Z7E8-1]
GeneIDi55585
KEGGihsa:55585
UCSCiuc001fff.2 human [Q7Z7E8-1]

Organism-specific databases

CTDi55585
EuPathDBiHostDB:ENSG00000160714.9
GeneCardsiUBE2Q1
H-InvDBiHIX0001106
HGNCiHGNC:15698 UBE2Q1
HPAiHPA063368
MIMi617429 gene
neXtProtiNX_Q7Z7E8
OpenTargetsiENSG00000160714
PharmGKBiPA38029
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0897 Eukaryota
ENOG410XRET LUCA
GeneTreeiENSGT00390000018573
HOVERGENiHBG061368
InParanoidiQ7Z7E8
KOiK10582
OMAiDSPLHND
OrthoDBiEOG091G0LGG
PhylomeDBiQ7Z7E8
TreeFamiTF313338

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiQ7Z7E8

Miscellaneous databases

ChiTaRSiUBE2Q1 human
EvolutionaryTraceiQ7Z7E8
GenomeRNAii55585
PROiPR:Q7Z7E8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000160714 Expressed in 228 organ(s), highest expression level in testis
CleanExiHS_UBE2Q1
GenevisibleiQ7Z7E8 HS

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 2 hits
PROSITEiView protein in PROSITE
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUB2Q1_HUMAN
AccessioniPrimary (citable) accession number: Q7Z7E8
Secondary accession number(s): B4DF92
, Q29SN7, Q3B841, Q5I0X2, Q6IS04, Q6P7P2, Q96MV4, Q9BVX5, Q9UGL6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: October 1, 2003
Last modified: September 12, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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