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Protein

Transcription initiation factor TFIID subunit 8

Gene

TAF8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo (By similarity).By similarity1 Publication

Miscellaneous

'Taube nuss' means 'empty nut' in German.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-6807505 RNA polymerase II transcribes snRNA genes

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 8
Alternative name(s):
Protein taube nuss
TBP-associated factor 43 kDa
TBP-associated factor 8
Transcription initiation factor TFIID 43 kDa subunit
Short name:
TAFII-43
Short name:
TAFII43
Short name:
hTAFII43
Gene namesi
Name:TAF8
Synonyms:TAFII43, TBN
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000137413.15
HGNCiHGNC:17300 TAF8
MIMi609514 gene
neXtProtiNX_Q7Z7C8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi129685
OpenTargetsiENSG00000137413
PharmGKBiPA162405144

Polymorphism and mutation databases

BioMutaiTAF8
DMDMi74723384

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00003153962 – 310Transcription initiation factor TFIID subunit 8Add BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei130PhosphothreonineCombined sources1
Modified residuei271PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ7Z7C8
MaxQBiQ7Z7C8
PaxDbiQ7Z7C8
PeptideAtlasiQ7Z7C8
PRIDEiQ7Z7C8
ProteomicsDBi69515
69516 [Q7Z7C8-2]
69517 [Q7Z7C8-4]

PTM databases

iPTMnetiQ7Z7C8
PhosphoSitePlusiQ7Z7C8

Expressioni

Inductioni

Induced with triglyceride accumulation.

Gene expression databases

BgeeiENSG00000137413 Expressed in 163 organ(s), highest expression level in amniotic fluid
CleanExiHS_TAF8
ExpressionAtlasiQ7Z7C8 baseline and differential
GenevisibleiQ7Z7C8 HS

Organism-specific databases

HPAiHPA031730
HPA031731
HPA031734

Interactioni

Subunit structurei

TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Interacts with TBP, TAF1, TAF6, TAF10, TAF11 and TAF13. Component also of a small TAF complex (SMAT) containing TAF8, TAF10 and SUPT7L. Forms a heterodimer with TAF10. Interaction with TAF10 is mediated mainly via its histone fold domain while interaction with SUPT7L is via its C-terminal region.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TAF10Q129625EBI-9089028,EBI-708376

GO - Molecular functioni

Protein-protein interaction databases

BioGridi126205, 33 interactors
ComplexPortaliCPX-900 SAGA complex
CPX-915 General transcription factor complex TFIID
CPX-930 General transcription factor complex TFIID, TAF4B variant
DIPiDIP-60591N
IntActiQ7Z7C8, 10 interactors
STRINGi9606.ENSP00000362068

Structurei

Secondary structure

1310
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ7Z7C8
SMRiQ7Z7C8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 102Histone-foldAdd BLAST68

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi294 – 307Nuclear localization signalSequence analysisAdd BLAST14

Domaini

Ectopic expression of the histone fold domain acts as a dominant-negative mutant resulting in differentiation inhibition.

Sequence similaritiesi

Belongs to the TAF8 family.Curated

Phylogenomic databases

eggNOGiKOG4336 Eukaryota
ENOG410ZRN5 LUCA
GeneTreeiENSGT00390000017567
HOGENOMiHOG000007034
HOVERGENiHBG105411
InParanoidiQ7Z7C8
KOiK14649
OMAiLFPTEDN
OrthoDBiEOG091G0O7T
PhylomeDBiQ7Z7C8
TreeFamiTF316311

Family and domain databases

CDDicd08049 TAF8, 1 hit
Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR006565 BTP
IPR009072 Histone-fold
IPR037818 TAF8
IPR019473 TFIID_su8_C
PANTHERiPTHR12321:SF102 PTHR12321:SF102, 1 hit
PfamiView protein in Pfam
PF07524 Bromo_TP, 1 hit
PF10406 TAF8_C, 1 hit
SMARTiView protein in SMART
SM00576 BTP, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q7Z7C8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MADAAATAGA GGSGTRSGSK QSTNPADNYH LARRRTLQVV VSSLLTEAGF
60 70 80 90 100
ESAEKASVET LTEMLQSYIS EIGRSAKSYC EHTARTQPTL SDIVVTLVEM
110 120 130 140 150
GFNVDTLPAY AKRSQRMVIT APPVTNQPVT PKALTAGQNR PHPPHIPSHF
160 170 180 190 200
PEFPDPHTYI KTPTYREPVS DYQVLREKAA SQRRDVERAL TRFMAKTGET
210 220 230 240 250
QSLFKDDVST FPLIAARPFT IPYLTALLPS ELEMQQMEET DSSEQDEQTD
260 270 280 290 300
TENLALHISM EDSGAEKENT SVLQQNPSLS GSRNGEENII DNPYLRPVKK
310
PKIRRKKSLS
Length:310
Mass (Da):34,262
Last modified:October 1, 2003 - v1
Checksum:i0F955828FE9FC0EA
GO
Isoform 2 (identifier: Q7Z7C8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     308-310: SLS → PDTF

Show »
Length:311
Mass (Da):34,436
Checksum:iE54B88DAF8FE9FC0
GO
Isoform 3 (identifier: Q7Z7C8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     261-310: EDSGAEKENT...PKIRRKKSLS → IESRSVTQAG...TSSITLICGR

Note: No experimental confirmation available.
Show »
Length:338
Mass (Da):37,411
Checksum:iB65123963AA96D63
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B4DZU5B4DZU5_HUMAN
Transcription initiation factor TFI...
TAF8
234Annotation score:
A0A0A0MRR3A0A0A0MRR3_HUMAN
Transcription initiation factor TFI...
TAF8
174Annotation score:
C9J7M8C9J7M8_HUMAN
Transcription initiation factor TFI...
TAF8
123Annotation score:
C9J856C9J856_HUMAN
Transcription initiation factor TFI...
TAF8
60Annotation score:

Sequence cautioni

The sequence BAB71460 differs from that shown. Reason: Frameshift at position 240.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti139N → D in BAB71460 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030548261 – 310EDSGA…KKSLS → IESRSVTQAGVQWQDLGSLQ PPPPGFKRFSSLSLLSSWNY RRILEPRRRTPLSCSRTPPC RVAGMGRRTSSITLICGR in isoform 3. 1 PublicationAdd BLAST50
Alternative sequenceiVSP_030549308 – 310SLS → PDTF in isoform 2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF465841 mRNA Translation: AAP47094.1
AK057383 mRNA Translation: BAB71460.1 Frameshift.
AL513008 Genomic DNA No translation available.
AL512274 Genomic DNA No translation available.
BC119678 mRNA Translation: AAI19679.1
BC119679 mRNA Translation: AAI19680.1
CCDSiCCDS43462.1 [Q7Z7C8-1]
RefSeqiNP_612639.2, NM_138572.2 [Q7Z7C8-1]
XP_011512598.1, XM_011514296.1 [Q7Z7C8-4]
UniGeneiHs.520122

Genome annotation databases

EnsembliENST00000372977; ENSP00000362068; ENSG00000137413 [Q7Z7C8-1]
ENST00000372982; ENSP00000362073; ENSG00000137413 [Q7Z7C8-4]
ENST00000456846; ENSP00000411900; ENSG00000137413 [Q7Z7C8-2]
ENST00000494547; ENSP00000417867; ENSG00000137413 [Q7Z7C8-4]
GeneIDi129685
KEGGihsa:129685
UCSCiuc003ors.4 human [Q7Z7C8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF465841 mRNA Translation: AAP47094.1
AK057383 mRNA Translation: BAB71460.1 Frameshift.
AL513008 Genomic DNA No translation available.
AL512274 Genomic DNA No translation available.
BC119678 mRNA Translation: AAI19679.1
BC119679 mRNA Translation: AAI19680.1
CCDSiCCDS43462.1 [Q7Z7C8-1]
RefSeqiNP_612639.2, NM_138572.2 [Q7Z7C8-1]
XP_011512598.1, XM_011514296.1 [Q7Z7C8-4]
UniGeneiHs.520122

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WV4X-ray1.91B25-120[»]
4WV6X-ray1.75B/C297-310[»]
5FURelectron microscopy8.50L1-310[»]
ProteinModelPortaliQ7Z7C8
SMRiQ7Z7C8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126205, 33 interactors
ComplexPortaliCPX-900 SAGA complex
CPX-915 General transcription factor complex TFIID
CPX-930 General transcription factor complex TFIID, TAF4B variant
DIPiDIP-60591N
IntActiQ7Z7C8, 10 interactors
STRINGi9606.ENSP00000362068

PTM databases

iPTMnetiQ7Z7C8
PhosphoSitePlusiQ7Z7C8

Polymorphism and mutation databases

BioMutaiTAF8
DMDMi74723384

Proteomic databases

EPDiQ7Z7C8
MaxQBiQ7Z7C8
PaxDbiQ7Z7C8
PeptideAtlasiQ7Z7C8
PRIDEiQ7Z7C8
ProteomicsDBi69515
69516 [Q7Z7C8-2]
69517 [Q7Z7C8-4]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000372977; ENSP00000362068; ENSG00000137413 [Q7Z7C8-1]
ENST00000372982; ENSP00000362073; ENSG00000137413 [Q7Z7C8-4]
ENST00000456846; ENSP00000411900; ENSG00000137413 [Q7Z7C8-2]
ENST00000494547; ENSP00000417867; ENSG00000137413 [Q7Z7C8-4]
GeneIDi129685
KEGGihsa:129685
UCSCiuc003ors.4 human [Q7Z7C8-1]

Organism-specific databases

CTDi129685
DisGeNETi129685
EuPathDBiHostDB:ENSG00000137413.15
GeneCardsiTAF8
HGNCiHGNC:17300 TAF8
HPAiHPA031730
HPA031731
HPA031734
MIMi609514 gene
neXtProtiNX_Q7Z7C8
OpenTargetsiENSG00000137413
PharmGKBiPA162405144
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4336 Eukaryota
ENOG410ZRN5 LUCA
GeneTreeiENSGT00390000017567
HOGENOMiHOG000007034
HOVERGENiHBG105411
InParanoidiQ7Z7C8
KOiK14649
OMAiLFPTEDN
OrthoDBiEOG091G0O7T
PhylomeDBiQ7Z7C8
TreeFamiTF316311

Enzyme and pathway databases

ReactomeiR-HSA-6807505 RNA polymerase II transcribes snRNA genes

Miscellaneous databases

ChiTaRSiTAF8 human
GeneWikiiTAF8
GenomeRNAii129685
PROiPR:Q7Z7C8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000137413 Expressed in 163 organ(s), highest expression level in amniotic fluid
CleanExiHS_TAF8
ExpressionAtlasiQ7Z7C8 baseline and differential
GenevisibleiQ7Z7C8 HS

Family and domain databases

CDDicd08049 TAF8, 1 hit
Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR006565 BTP
IPR009072 Histone-fold
IPR037818 TAF8
IPR019473 TFIID_su8_C
PANTHERiPTHR12321:SF102 PTHR12321:SF102, 1 hit
PfamiView protein in Pfam
PF07524 Bromo_TP, 1 hit
PF10406 TAF8_C, 1 hit
SMARTiView protein in SMART
SM00576 BTP, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTAF8_HUMAN
AccessioniPrimary (citable) accession number: Q7Z7C8
Secondary accession number(s): Q5T0K1, Q8N4R9, Q96M52
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2003
Last modified: September 12, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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