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Protein

N-terminal EF-hand calcium-binding protein 2

Gene

NECAB2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May act as a signaling scaffold protein that senses intracellular calcium. Can modulate ligand-induced internalization of ADORA2A and coupling efficiency of mGluR5/GRM5; for both receptors may regulate signaling activity such as promoting MAPK1/3 (ERK1/2) activation.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi73 – 841PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi107 – 1182PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • A2A adenosine receptor binding Source: UniProtKB
  • calcium ion binding Source: InterPro
  • identical protein binding Source: IntAct
  • type 5 metabotropic glutamate receptor binding Source: UniProtKB

GO - Biological processi

Keywordsi

LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
N-terminal EF-hand calcium-binding protein 2
Short name:
EF-hand calcium-binding protein 2
Alternative name(s):
Neuronal calcium-binding protein 2
Synaptotagmin-interacting protein 2
Short name:
Stip-2
Gene namesi
Name:NECAB2
Synonyms:EFCBP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000103154.9
HGNCiHGNC:23746 NECAB2
neXtProtiNX_Q7Z6G3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi54550
OpenTargetsiENSG00000103154
PharmGKBiPA162397412

Polymorphism and mutation databases

BioMutaiNECAB2
DMDMi74762441

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002826131 – 386N-terminal EF-hand calcium-binding protein 2Add BLAST386

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10Omega-N-methylarginineBy similarity1
Modified residuei42Asymmetric dimethylarginineBy similarity1

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiQ7Z6G3
PeptideAtlasiQ7Z6G3
PRIDEiQ7Z6G3
ProteomicsDBi69400

PTM databases

PhosphoSitePlusiQ7Z6G3

Expressioni

Tissue specificityi

Expressed in brain. Expressed in the spinal dorsal horn with especially strong expression in lamina IIi; found in excitory synaptic boutons and in ependymal cells (at protein level).2 Publications

Gene expression databases

BgeeiENSG00000103154 Expressed in 139 organ(s), highest expression level in amygdala
CleanExiHS_NECAB2
ExpressionAtlasiQ7Z6G3 baseline and differential
GenevisibleiQ7Z6G3 HS

Organism-specific databases

HPAiCAB068211
HPA013998
HPA014144

Interactioni

Subunit structurei

Interacts (calcium-dependent) with ADORA2A and GRM5.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi120035, 76 interactors
IntActiQ7Z6G3, 82 interactors
MINTiQ7Z6G3

Structurei

3D structure databases

ProteinModelPortaliQ7Z6G3
SMRiQ7Z6G3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 95EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini94 – 129EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini286 – 375ABMAdd BLAST90

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili170 – 201Sequence analysisAdd BLAST32

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IH3U Eukaryota
ENOG4111GJU LUCA
GeneTreeiENSGT00390000009734
HOGENOMiHOG000008120
HOVERGENiHBG050433
InParanoidiQ7Z6G3
OMAiRDCFHVT
OrthoDBiEOG091G0DWL
PhylomeDBiQ7Z6G3
TreeFamiTF331029

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR007138 ABM_dom
IPR011008 Dimeric_a/b-barrel
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR039862 NECAB1/2/3
PANTHERiPTHR12178 PTHR12178, 1 hit
PfamiView protein in Pfam
PF03992 ABM, 1 hit
PF13202 EF-hand_5, 1 hit
PF13833 EF-hand_8, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
SSF54909 SSF54909, 1 hit
PROSITEiView protein in PROSITE
PS51725 ABM, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q7Z6G3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MCERAARLCR AGAHRLLREP PQQGRALGGL LRWVGARMGE PRESLAPAAP
60 70 80 90 100
ADPGPASPRG GTAVILDIFR RADKNDDGKL SLEEFQLFFA DGVLNEKELE
110 120 130 140 150
DLFHTIDSDN TNHVDTKELC DYFVDHMGDY EDVLASLETL NHSVLKAMGY
160 170 180 190 200
TKKVYEGGSN VDQFVTRFLL KETANQIQSL LSSVESAVEA IEEQTSQLRQ
210 220 230 240 250
NHIKPSHSAA QTWCGSPTPA SAPNHKLMAM EQGKTLPSAT EDAKEEGLEA
260 270 280 290 300
QISRLAELIG RLESKALWFD LQQRLSDEDG TNMHLQLVRQ EMAVCPEQLS
310 320 330 340 350
EFLDSLRQYL RGTTGVRNCF HITAVRLSDG FTFVIYEFWE TEEAWKRHLQ
360 370 380
SPLCKAFRHV KVDTLSQPEA LSRILVPAAW CTVGRD
Length:386
Mass (Da):43,194
Last modified:October 1, 2003 - v1
Checksum:iD5F32B9AACF1A1C1
GO
Isoform 2 (identifier: Q7Z6G3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-121: MCERAARLCR...NHVDTKELCD → MMMGSCPWRN...FTRLTLTTPN

Show »
Length:303
Mass (Da):34,322
Checksum:i0D700F56055354EF
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BPH6H3BPH6_HUMAN
N-terminal EF-hand calcium-binding ...
NECAB2
183Annotation score:
H3BQC7H3BQC7_HUMAN
N-terminal EF-hand calcium-binding ...
NECAB2
62Annotation score:

Sequence cautioni

The sequence AAI31616 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC86948 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048639233G → S. Corresponds to variant dbSNP:rs2292323Ensembl.1
Natural variantiVAR_048640235T → S. Corresponds to variant dbSNP:rs2292324Ensembl.1
Natural variantiVAR_048641308Q → H. Corresponds to variant dbSNP:rs2292329Ensembl.1
Natural variantiVAR_048642353L → V. Corresponds to variant dbSNP:rs2271298Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0589361 – 121MCERA…KELCD → MMMGSCPWRNSSSSLQMASL MRKNWRISFTRLTLTTPN in isoform 2. Add BLAST121

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY299331 mRNA Translation: AAP57260.1
AK127376 mRNA Translation: BAC86948.1 Different initiation.
AC040169 Genomic DNA No translation available.
BC016979 mRNA No translation available.
BC131615 mRNA Translation: AAI31616.1 Different initiation.
AF193758 mRNA Translation: AAG28414.1
AF070637 mRNA Translation: AAC25392.1
CCDSiCCDS10940.1 [Q7Z6G3-1]
CCDS82019.1 [Q7Z6G3-2]
RefSeqiNP_001316678.1, NM_001329749.1 [Q7Z6G3-2]
NP_061938.2, NM_019065.2 [Q7Z6G3-1]
UniGeneiHs.140950

Genome annotation databases

EnsembliENST00000305202; ENSP00000307449; ENSG00000103154 [Q7Z6G3-1]
ENST00000565691; ENSP00000457354; ENSG00000103154 [Q7Z6G3-2]
GeneIDi54550
KEGGihsa:54550
UCSCiuc002fhd.4 human [Q7Z6G3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY299331 mRNA Translation: AAP57260.1
AK127376 mRNA Translation: BAC86948.1 Different initiation.
AC040169 Genomic DNA No translation available.
BC016979 mRNA No translation available.
BC131615 mRNA Translation: AAI31616.1 Different initiation.
AF193758 mRNA Translation: AAG28414.1
AF070637 mRNA Translation: AAC25392.1
CCDSiCCDS10940.1 [Q7Z6G3-1]
CCDS82019.1 [Q7Z6G3-2]
RefSeqiNP_001316678.1, NM_001329749.1 [Q7Z6G3-2]
NP_061938.2, NM_019065.2 [Q7Z6G3-1]
UniGeneiHs.140950

3D structure databases

ProteinModelPortaliQ7Z6G3
SMRiQ7Z6G3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120035, 76 interactors
IntActiQ7Z6G3, 82 interactors
MINTiQ7Z6G3

PTM databases

PhosphoSitePlusiQ7Z6G3

Polymorphism and mutation databases

BioMutaiNECAB2
DMDMi74762441

Proteomic databases

PaxDbiQ7Z6G3
PeptideAtlasiQ7Z6G3
PRIDEiQ7Z6G3
ProteomicsDBi69400

Protocols and materials databases

DNASUi54550
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000305202; ENSP00000307449; ENSG00000103154 [Q7Z6G3-1]
ENST00000565691; ENSP00000457354; ENSG00000103154 [Q7Z6G3-2]
GeneIDi54550
KEGGihsa:54550
UCSCiuc002fhd.4 human [Q7Z6G3-1]

Organism-specific databases

CTDi54550
DisGeNETi54550
EuPathDBiHostDB:ENSG00000103154.9
GeneCardsiNECAB2
HGNCiHGNC:23746 NECAB2
HPAiCAB068211
HPA013998
HPA014144
neXtProtiNX_Q7Z6G3
OpenTargetsiENSG00000103154
PharmGKBiPA162397412
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IH3U Eukaryota
ENOG4111GJU LUCA
GeneTreeiENSGT00390000009734
HOGENOMiHOG000008120
HOVERGENiHBG050433
InParanoidiQ7Z6G3
OMAiRDCFHVT
OrthoDBiEOG091G0DWL
PhylomeDBiQ7Z6G3
TreeFamiTF331029

Miscellaneous databases

ChiTaRSiNECAB2 human
GeneWikiiEFCBP2
GenomeRNAii54550
PROiPR:Q7Z6G3

Gene expression databases

BgeeiENSG00000103154 Expressed in 139 organ(s), highest expression level in amygdala
CleanExiHS_NECAB2
ExpressionAtlasiQ7Z6G3 baseline and differential
GenevisibleiQ7Z6G3 HS

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR007138 ABM_dom
IPR011008 Dimeric_a/b-barrel
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR039862 NECAB1/2/3
PANTHERiPTHR12178 PTHR12178, 1 hit
PfamiView protein in Pfam
PF03992 ABM, 1 hit
PF13202 EF-hand_5, 1 hit
PF13833 EF-hand_8, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
SSF54909 SSF54909, 1 hit
PROSITEiView protein in PROSITE
PS51725 ABM, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiNECA2_HUMAN
AccessioniPrimary (citable) accession number: Q7Z6G3
Secondary accession number(s): A2RRG3
, H3BTW2, O75547, Q6ZSK0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 1, 2003
Last modified: November 7, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
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Main funding by: National Institutes of Health

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