UniProtKB - Q7Z698 (SPRE2_HUMAN)
Sprouty-related, EVH1 domain-containing protein 2
SPRED2
Functioni
GO - Molecular functioni
- protein kinase binding Source: BHF-UCL
- protein serine/threonine kinase inhibitor activity Source: BHF-UCL
- stem cell factor receptor binding Source: UniProtKB
GO - Biological processi
- fibroblast growth factor receptor signaling pathway Source: Reactome
- inactivation of MAPK activity Source: UniProtKB
- multicellular organism development Source: InterPro
- negative regulation of epithelial to mesenchymal transition Source: UniProtKB
- negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
- negative regulation of lens fiber cell differentiation Source: UniProtKB
- negative regulation of peptidyl-threonine phosphorylation Source: BHF-UCL
- negative regulation of transforming growth factor beta receptor signaling pathway Source: UniProtKB
- positive regulation of DNA damage response, signal transduction by p53 class mediator Source: BHF-UCL
- regulation of protein deacetylation Source: BHF-UCL
Enzyme and pathway databases
PathwayCommonsi | Q7Z698 |
Reactomei | R-HSA-5658442, Regulation of RAS by GAPs R-HSA-5658623, FGFRL1 modulation of FGFR1 signaling R-HSA-6802953, RAS signaling downstream of NF1 loss-of-function variants |
Names & Taxonomyi
Protein namesi | Recommended name: Sprouty-related, EVH1 domain-containing protein 2Short name: Spred-2 |
Gene namesi | Name:SPRED2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17722, SPRED2 |
MIMi | 609292, gene |
neXtProti | NX_Q7Z698 |
VEuPathDBi | HostDB:ENSG00000198369.9 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Other locations
- secretory vesicle membrane 1 Publication; Peripheral membrane protein Curated; Cytoplasmic side Curated
- Cytoplasm 1 Publication
Note: Detected in the cytoplasm of the stratum spinosum cells, where it is associated with cytoplasmic vesicles that are supposed to be secretory granules.1 Publication
Cytosol
- cytosol Source: Reactome
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- transport vesicle membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoplasmic vesicle, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 228 | Y → F: Reduces ubiquitination and CBL-induced phosphorylation; when associated with F-231. 1 Publication | 1 | |
Mutagenesisi | 231 | Y → F: Reduces ubiquitination and CBL-induced phosphorylation; when associated with F-228. 1 Publication | 1 | |
Mutagenesisi | 240 | Y → F: No affect on phosphorylation or ubiquitination. 1 Publication | 1 | |
Mutagenesisi | 251 | Y → F: No affect on phosphorylation or ubiquitination. 1 Publication | 1 | |
Mutagenesisi | 264 | Y → F: No affect on phosphorylation or ubiquitination; when associated with F-266. 1 Publication | 1 | |
Mutagenesisi | 266 | Y → F: No affect on phosphorylation or ubiquitination; when associated with F-264. 1 Publication | 1 | |
Mutagenesisi | 268 | Y → F: No affect on phosphorylation or ubiquitination. 1 Publication | 1 | |
Mutagenesisi | 311 | Y → F: No affect on phosphorylation or ubiquitination. 1 Publication | 1 | |
Mutagenesisi | 351 | Y → F: No affect on phosphorylation or ubiquitination. 1 Publication | 1 | |
Mutagenesisi | 394 | Y → F: No affect on phosphorylation or ubiquitination. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 200734 |
OpenTargetsi | ENSG00000198369 |
PharmGKBi | PA134956365 |
Miscellaneous databases
Pharosi | Q7Z698, Tbio |
Genetic variation databases
BioMutai | SPRED2 |
DMDMi | 110825745 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076910 | 1 – 418 | Sprouty-related, EVH1 domain-containing protein 2Add BLAST | 418 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 206 | PhosphoserineCombined sources | 1 | |
Modified residuei | 228 | Phosphotyrosine1 Publication | 1 | |
Modified residuei | 231 | Phosphotyrosine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q7Z698 |
jPOSTi | Q7Z698 |
MassIVEi | Q7Z698 |
MaxQBi | Q7Z698 |
PaxDbi | Q7Z698 |
PeptideAtlasi | Q7Z698 |
PRIDEi | Q7Z698 |
ProteomicsDBi | 69384 [Q7Z698-1] 69385 [Q7Z698-2] |
PTM databases
iPTMneti | Q7Z698 |
PhosphoSitePlusi | Q7Z698 |
SwissPalmi | Q7Z698 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000198369, Expressed in forebrain and 220 other tissues |
ExpressionAtlasi | Q7Z698, baseline and differential |
Genevisiblei | Q7Z698, HS |
Organism-specific databases
HPAi | ENSG00000198369, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer and heterodimer (PubMed:15683364). Able to interact with SPRED1 to form heterodimers (PubMed:15683364).
Interacts with RAS (By similarity). May interact with ZDHHC13 (via ANK repeats) and ZDHHC17 (via ANK repeats) (By similarity).
Interacts with TESK1 (By similarity).
By similarity1 PublicationBinary interactionsi
Hide detailsQ7Z698
GO - Molecular functioni
- protein kinase binding Source: BHF-UCL
- stem cell factor receptor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 128344, 50 interactors |
IntActi | Q7Z698, 56 interactors |
MINTi | Q7Z698 |
STRINGi | 9606.ENSP00000348753 |
Miscellaneous databases
RNActi | Q7Z698, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q7Z698 |
SMRi | Q7Z698 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q7Z698 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 122 | WH1PROSITE-ProRule annotationAdd BLAST | 118 | |
Domaini | 201 – 257 | KBDPROSITE-ProRule annotationAdd BLAST | 57 | |
Domaini | 308 – 416 | SPRPROSITE-ProRule annotationAdd BLAST | 109 |
Phylogenomic databases
eggNOGi | KOG4590, Eukaryota |
GeneTreei | ENSGT00940000156841 |
HOGENOMi | CLU_038867_1_1_1 |
InParanoidi | Q7Z698 |
OrthoDBi | 1094638at2759 |
PhylomeDBi | Q7Z698 |
TreeFami | TF321411 |
Family and domain databases
CDDi | cd10574, EVH1_SPRED-like, 1 hit |
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR023337, KBD IPR011993, PH-like_dom_sf IPR041937, SPRE_EVH1 IPR007875, Sprouty IPR000697, WH1/EVH1_dom |
Pfami | View protein in Pfam PF05210, Sprouty, 1 hit PF00568, WH1, 1 hit |
SMARTi | View protein in SMART SM00461, WH1, 1 hit |
PROSITEi | View protein in PROSITE PS51488, KBD, 1 hit PS51227, SPR, 1 hit PS50229, WH1, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTEETHPDDD SYIVRVKAVV MTRDDSSGGW FPQEGGGISR VGVCKVMHPE
60 70 80 90 100
GNGRSGFLIH GERQKDKLVV LECYVRKDLV YTKANPTFHH WKVDNRKFGL
110 120 130 140 150
TFQSPADARA FDRGVRKAIE DLIEGSTTSS STIHNEAELG DDDVFTTATD
160 170 180 190 200
SSSNSSQKRE QPTRTISSPT SCEHRRIYTL GHLHDSYPTD HYHLDQPMPR
210 220 230 240 250
PYRQVSFPDD DEEIVRINPR EKIWMTGYED YRHAPVRGKY PDPSEDADSS
260 270 280 290 300
YVRFAKGEVP KHDYNYPYVD SSDFGLGEDP KGRGGSVIKT QPSRGKSRRR
310 320 330 340 350
KEDGERSRCV YCRDMFNHEE NRRGHCQDAP DSVRTCIRRV SCMWCADSML
360 370 380 390 400
YHCMSDPEGD YTDPCSCDTS DEKFCLRWMA LIALSFLAPC MCCYLPLRAC
410
YHCGVMCRCC GGKHKAAA
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JG63 | C9JG63_HUMAN | Sprouty-related, EVH1 domain-contai... | SPRED2 | 432 | Annotation score: | ||
H7C2T4 | H7C2T4_HUMAN | Sprouty-related, EVH1 domain-contai... | SPRED2 | 233 | Annotation score: | ||
C9J623 | C9J623_HUMAN | Sprouty-related, EVH1 domain-contai... | SPRED2 | 116 | Annotation score: | ||
H7C3Y6 | H7C3Y6_HUMAN | Sprouty-related, EVH1 domain-contai... | SPRED2 | 103 | Annotation score: | ||
H7C065 | H7C065_HUMAN | Sprouty-related, EVH1 domain-contai... | SPRED2 | 47 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 33 | Q → R in BAH12943 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 202 | Y → C in AAP59415 (PubMed:15683364).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_043755 | 1 – 9 | MTEETHPDD → MASPGS in isoform 2. 1 Publication | 9 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY299090 mRNA Translation: AAP59415.1 AK299079 mRNA Translation: BAH12943.1 AC012370 Genomic DNA No translation available. AC097503 Genomic DNA No translation available. CH471053 Genomic DNA Translation: EAW99905.1 CH471053 Genomic DNA Translation: EAW99906.1 BC111495 mRNA Translation: AAI11496.1 BC130292 mRNA Translation: AAI30293.1 BC136334 mRNA Translation: AAI36335.1 |
CCDSi | CCDS33211.1 [Q7Z698-1] CCDS46308.1 [Q7Z698-2] |
RefSeqi | NP_001121682.1, NM_001128210.1 [Q7Z698-2] NP_861449.2, NM_181784.2 [Q7Z698-1] |
Genome annotation databases
Ensembli | ENST00000356388; ENSP00000348753; ENSG00000198369 [Q7Z698-1] ENST00000443619; ENSP00000393697; ENSG00000198369 [Q7Z698-2] |
GeneIDi | 200734 |
KEGGi | hsa:200734 |
UCSCi | uc002sdr.5, human [Q7Z698-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY299090 mRNA Translation: AAP59415.1 AK299079 mRNA Translation: BAH12943.1 AC012370 Genomic DNA No translation available. AC097503 Genomic DNA No translation available. CH471053 Genomic DNA Translation: EAW99905.1 CH471053 Genomic DNA Translation: EAW99906.1 BC111495 mRNA Translation: AAI11496.1 BC130292 mRNA Translation: AAI30293.1 BC136334 mRNA Translation: AAI36335.1 |
CCDSi | CCDS33211.1 [Q7Z698-1] CCDS46308.1 [Q7Z698-2] |
RefSeqi | NP_001121682.1, NM_001128210.1 [Q7Z698-2] NP_861449.2, NM_181784.2 [Q7Z698-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2JP2 | NMR | - | A | 1-124 | [»] | |
BMRBi | Q7Z698 | |||||
SMRi | Q7Z698 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 128344, 50 interactors |
IntActi | Q7Z698, 56 interactors |
MINTi | Q7Z698 |
STRINGi | 9606.ENSP00000348753 |
PTM databases
iPTMneti | Q7Z698 |
PhosphoSitePlusi | Q7Z698 |
SwissPalmi | Q7Z698 |
Genetic variation databases
BioMutai | SPRED2 |
DMDMi | 110825745 |
Proteomic databases
EPDi | Q7Z698 |
jPOSTi | Q7Z698 |
MassIVEi | Q7Z698 |
MaxQBi | Q7Z698 |
PaxDbi | Q7Z698 |
PeptideAtlasi | Q7Z698 |
PRIDEi | Q7Z698 |
ProteomicsDBi | 69384 [Q7Z698-1] 69385 [Q7Z698-2] |
Protocols and materials databases
Antibodypediai | 30873, 141 antibodies |
DNASUi | 200734 |
Genome annotation databases
Ensembli | ENST00000356388; ENSP00000348753; ENSG00000198369 [Q7Z698-1] ENST00000443619; ENSP00000393697; ENSG00000198369 [Q7Z698-2] |
GeneIDi | 200734 |
KEGGi | hsa:200734 |
UCSCi | uc002sdr.5, human [Q7Z698-1] |
Organism-specific databases
CTDi | 200734 |
DisGeNETi | 200734 |
GeneCardsi | SPRED2 |
HGNCi | HGNC:17722, SPRED2 |
HPAi | ENSG00000198369, Low tissue specificity |
MIMi | 609292, gene |
neXtProti | NX_Q7Z698 |
OpenTargetsi | ENSG00000198369 |
PharmGKBi | PA134956365 |
VEuPathDBi | HostDB:ENSG00000198369.9 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4590, Eukaryota |
GeneTreei | ENSGT00940000156841 |
HOGENOMi | CLU_038867_1_1_1 |
InParanoidi | Q7Z698 |
OrthoDBi | 1094638at2759 |
PhylomeDBi | Q7Z698 |
TreeFami | TF321411 |
Enzyme and pathway databases
PathwayCommonsi | Q7Z698 |
Reactomei | R-HSA-5658442, Regulation of RAS by GAPs R-HSA-5658623, FGFRL1 modulation of FGFR1 signaling R-HSA-6802953, RAS signaling downstream of NF1 loss-of-function variants |
Miscellaneous databases
BioGRID-ORCSi | 200734, 15 hits in 880 CRISPR screens |
ChiTaRSi | SPRED2, human |
EvolutionaryTracei | Q7Z698 |
GeneWikii | SPRED2 |
GenomeRNAii | 200734 |
Pharosi | Q7Z698, Tbio |
PROi | PR:Q7Z698 |
RNActi | Q7Z698, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000198369, Expressed in forebrain and 220 other tissues |
ExpressionAtlasi | Q7Z698, baseline and differential |
Genevisiblei | Q7Z698, HS |
Family and domain databases
CDDi | cd10574, EVH1_SPRED-like, 1 hit |
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR023337, KBD IPR011993, PH-like_dom_sf IPR041937, SPRE_EVH1 IPR007875, Sprouty IPR000697, WH1/EVH1_dom |
Pfami | View protein in Pfam PF05210, Sprouty, 1 hit PF00568, WH1, 1 hit |
SMARTi | View protein in SMART SM00461, WH1, 1 hit |
PROSITEi | View protein in PROSITE PS51488, KBD, 1 hit PS51227, SPR, 1 hit PS50229, WH1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPRE2_HUMAN | |
Accessioni | Q7Z698Primary (citable) accession number: Q7Z698 Secondary accession number(s): A1L3V4 Q2NKX6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 4, 2005 |
Last sequence update: | July 25, 2006 | |
Last modified: | February 10, 2021 | |
This is version 154 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM