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Protein

BRCA1-associated protein

Gene

BRAP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.1 Publication1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri264 – 304RING-typePROSITE-ProRule annotationCuratedAdd BLAST41
Zinc fingeri315 – 376UBP-typePROSITE-ProRule annotationAdd BLAST62

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-5673000 RAF activation
R-HSA-5675221 Negative regulation of MAPK pathway
R-HSA-6802946 Signaling by moderate kinase activity BRAF mutants
R-HSA-6802949 Signaling by RAS mutants
R-HSA-6802955 Paradoxical activation of RAF signaling by kinase inactive BRAF
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
BRCA1-associated protein (EC:2.3.2.271 Publication)
Alternative name(s):
BRAP2
Impedes mitogenic signal propagation
Short name:
IMP
RING finger protein 52
RING-type E3 ubiquitin transferase BRAP2Curated
Renal carcinoma antigen NY-REN-63
Gene namesi
Name:BRAPImported
Synonyms:RNF52
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000089234.15
HGNCiHGNC:1099 BRAP
MIMi604986 gene
neXtProtiNX_Q7Z569

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi264C → A: Loss of E3 ubiquitin-protein ligase activity. 1 Publication1

Organism-specific databases

DisGeNETi8315
OpenTargetsiENSG00000089234
PharmGKBiPA25410

Polymorphism and mutation databases

BioMutaiBRAP
DMDMi296434410

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000558251 – 592BRCA1-associated proteinAdd BLAST592

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52PhosphoserineCombined sources1
Modified residuei97PhosphoserineCombined sources1
Modified residuei117PhosphoserineCombined sources1
Modified residuei119PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ7Z569
MaxQBiQ7Z569
PaxDbiQ7Z569
PeptideAtlasiQ7Z569
PRIDEiQ7Z569
ProteomicsDBi69260

PTM databases

iPTMnetiQ7Z569
PhosphoSitePlusiQ7Z569

Expressioni

Tissue specificityi

Expressed in breast epithelial cell lines.1 Publication

Gene expression databases

BgeeiENSG00000089234 Expressed in 225 organ(s), highest expression level in left testis
CleanExiHS_BRAP
ExpressionAtlasiQ7Z569 baseline and differential
GenevisibleiQ7Z569 HS

Organism-specific databases

HPAiHPA040357
HPA058820

Interactioni

Subunit structurei

Interacts with the nuclear localization signal of BRCA1 and with the N-terminal of KSR1. The C-terminal portion of BCRA1 interacts with DDB1.3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi113912, 56 interactors
IntActiQ7Z569, 43 interactors
MINTiQ7Z569
STRINGi9606.ENSP00000403524

Structurei

3D structure databases

ProteinModelPortaliQ7Z569
SMRiQ7Z569
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili429 – 537Sequence analysisAdd BLAST109

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri264 – 304RING-typePROSITE-ProRule annotationCuratedAdd BLAST41
Zinc fingeri315 – 376UBP-typePROSITE-ProRule annotationAdd BLAST62

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0804 Eukaryota
ENOG410XSS2 LUCA
GeneTreeiENSGT00500000044909
HOGENOMiHOG000190616
HOVERGENiHBG050729
InParanoidiQ7Z569
KOiK10632
OMAiNQYMALI
OrthoDBiEOG091G07BQ
PhylomeDBiQ7Z569
TreeFamiTF313622

Family and domain databases

CDDicd12718 RRM_BRAP2, 1 hit
Gene3Di3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR011422 BRAP2
IPR034932 BRAP2_RRM
IPR035979 RBD_domain_sf
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR001607 Znf_UBP
PfamiView protein in Pfam
PF07576 BRAP2, 1 hit
PF13639 zf-RING_2, 1 hit
PF02148 zf-UBP, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD017029 BRAP2, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SM00290 ZnF_UBP, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit
PS50271 ZF_UBP, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q7Z569-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSVSLVVIRL ELAEHSPVPA GFGFSAAAGE MSDEEIKKTT LASAVACLEG
60 70 80 90 100
KSPGEKVAII HQHLGRREMT DVIIETMKSN PDELKTTVEE RKSSEASPTA
110 120 130 140 150
QRSKDHSKEC INAAPDSPSK QLPDQISFFS GNPSVEIVHG IMHLYKTNKM
160 170 180 190 200
TSLKEDVRRS AMLCILTVPA AMTSHDLMKF VAPFNEVIEQ MKIIRDSTPN
210 220 230 240 250
QYMVLIKFRA QADADSFYMT CNGRQFNSIE DDVCQLVYVE RAEVLKSEDG
260 270 280 290 300
ASLPVMDLTE LPKCTVCLER MDESVNGILT TLCNHSFHSQ CLQRWDDTTC
310 320 330 340 350
PVCRYCQTPE PVEENKCFEC GVQENLWICL ICGHIGCGRY VSRHAYKHFE
360 370 380 390 400
ETQHTYAMQL TNHRVWDYAG DNYVHRLVAS KTDGKIVQYE CEGDTCQEEK
410 420 430 440 450
IDALQLEYSY LLTSQLESQR IYWENKIVRI EKDTAEEINN MKTKFKETIE
460 470 480 490 500
KCDNLEHKLN DLLKEKQSVE RKCTQLNTKV AKLTNELKEE QEMNKCLRAN
510 520 530 540 550
QVLLQNKLKE EERVLKETCD QKDLQITEIQ EQLRDVMFYL ETQQKINHLP
560 570 580 590
AETRQEIQEG QINIAMASAS SPASSGGSGK LPSRKGRSKR GK
Length:592
Mass (Da):67,305
Last modified:May 18, 2010 - v2
Checksum:i70CFEAB560D1952C
GO
Isoform 2 (identifier: Q7Z569-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-141: Missing.
     212-249: Missing.

Note: No experimental confirmation available.
Show »
Length:413
Mass (Da):47,853
Checksum:iF54A77311160F312
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3KNN7J3KNN7_HUMAN
BRCA1-associated protein
BRAP
562Annotation score:

Sequence cautioni

The sequence AAC24200 differs from that shown. Reason: Frameshift at position 556.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti186E → D in AAC24200 (PubMed:9497340).Curated1
Sequence conflicti186E → D in AAP93638 (PubMed:14724641).Curated1
Sequence conflicti356Y → H in AAB88538 (PubMed:10777491).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0558811 – 141Missing in isoform 2. 1 PublicationAdd BLAST141
Alternative sequenceiVSP_055882212 – 249Missing in isoform 2. 1 PublicationAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035620 mRNA Translation: AAC24200.1 Frameshift.
AY332222 mRNA Translation: AAP93638.1
AK299326 mRNA Translation: BAG61333.1
AC002996 Genomic DNA No translation available.
AC137055 Genomic DNA No translation available.
CH471054 Genomic DNA Translation: EAW97972.1
BC136698 mRNA Translation: AAI36699.1
BC136699 mRNA Translation: AAI36700.1
AF035950 mRNA Translation: AAB88538.1
CCDSiCCDS9154.1 [Q7Z569-1]
RefSeqiNP_006759.3, NM_006768.4 [Q7Z569-1]
UniGeneiHs.530940

Genome annotation databases

EnsembliENST00000419234; ENSP00000403524; ENSG00000089234 [Q7Z569-1]
GeneIDi8315
KEGGihsa:8315
UCSCiuc001tsn.4 human [Q7Z569-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035620 mRNA Translation: AAC24200.1 Frameshift.
AY332222 mRNA Translation: AAP93638.1
AK299326 mRNA Translation: BAG61333.1
AC002996 Genomic DNA No translation available.
AC137055 Genomic DNA No translation available.
CH471054 Genomic DNA Translation: EAW97972.1
BC136698 mRNA Translation: AAI36699.1
BC136699 mRNA Translation: AAI36700.1
AF035950 mRNA Translation: AAB88538.1
CCDSiCCDS9154.1 [Q7Z569-1]
RefSeqiNP_006759.3, NM_006768.4 [Q7Z569-1]
UniGeneiHs.530940

3D structure databases

ProteinModelPortaliQ7Z569
SMRiQ7Z569
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113912, 56 interactors
IntActiQ7Z569, 43 interactors
MINTiQ7Z569
STRINGi9606.ENSP00000403524

PTM databases

iPTMnetiQ7Z569
PhosphoSitePlusiQ7Z569

Polymorphism and mutation databases

BioMutaiBRAP
DMDMi296434410

Proteomic databases

EPDiQ7Z569
MaxQBiQ7Z569
PaxDbiQ7Z569
PeptideAtlasiQ7Z569
PRIDEiQ7Z569
ProteomicsDBi69260

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000419234; ENSP00000403524; ENSG00000089234 [Q7Z569-1]
GeneIDi8315
KEGGihsa:8315
UCSCiuc001tsn.4 human [Q7Z569-1]

Organism-specific databases

CTDi8315
DisGeNETi8315
EuPathDBiHostDB:ENSG00000089234.15
GeneCardsiBRAP
H-InvDBiHIX0036684
HGNCiHGNC:1099 BRAP
HPAiHPA040357
HPA058820
MIMi604986 gene
neXtProtiNX_Q7Z569
OpenTargetsiENSG00000089234
PharmGKBiPA25410
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0804 Eukaryota
ENOG410XSS2 LUCA
GeneTreeiENSGT00500000044909
HOGENOMiHOG000190616
HOVERGENiHBG050729
InParanoidiQ7Z569
KOiK10632
OMAiNQYMALI
OrthoDBiEOG091G07BQ
PhylomeDBiQ7Z569
TreeFamiTF313622

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-HSA-5673000 RAF activation
R-HSA-5675221 Negative regulation of MAPK pathway
R-HSA-6802946 Signaling by moderate kinase activity BRAF mutants
R-HSA-6802949 Signaling by RAS mutants
R-HSA-6802955 Paradoxical activation of RAF signaling by kinase inactive BRAF

Miscellaneous databases

ChiTaRSiBRAP human
GenomeRNAii8315
PROiPR:Q7Z569
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000089234 Expressed in 225 organ(s), highest expression level in left testis
CleanExiHS_BRAP
ExpressionAtlasiQ7Z569 baseline and differential
GenevisibleiQ7Z569 HS

Family and domain databases

CDDicd12718 RRM_BRAP2, 1 hit
Gene3Di3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR011422 BRAP2
IPR034932 BRAP2_RRM
IPR035979 RBD_domain_sf
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR001607 Znf_UBP
PfamiView protein in Pfam
PF07576 BRAP2, 1 hit
PF13639 zf-RING_2, 1 hit
PF02148 zf-UBP, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD017029 BRAP2, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SM00290 ZnF_UBP, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit
PS50271 ZF_UBP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBRAP_HUMAN
AccessioniPrimary (citable) accession number: Q7Z569
Secondary accession number(s): B4DRM1
, B9EGS8, O43238, O75341
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: May 18, 2010
Last modified: September 12, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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