UniProtKB - Q7Z478 (DHX29_HUMAN)
Protein
ATP-dependent RNA helicase DHX29
Gene
DHX29
Organism
Homo sapiens (Human)
Status
Functioni
ATP-binding RNA helicase involved in translation initiation. Part of the 43S pre-initiation complex that is required for efficient initiation on mRNAs of higher eukaryotes with structured 5'-UTRs by promoting efficient NTPase-dependent 48S complex formation. Specifically binds to the 40S ribosome near the mRNA entrance. Does not possess a processive helicase activity.UniRule annotation2 Publications
Catalytic activityi
- EC:3.6.4.13UniRule annotation
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 595 – 602 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- cadherin binding Source: BHF-UCL
- nucleoside-triphosphatase activity Source: CACAO
- ribosomal small subunit binding Source: UniProtKB
- RNA binding Source: UniProtKB
- RNA helicase activity Source: UniProtKB-UniRule
- translation activator activity Source: CACAO
- translation initiation factor activity Source: UniProtKB-KW
GO - Biological processi
- formation of translation preinitiation complex Source: CACAO
- positive regulation of translational initiation Source: UniProtKB-UniRule
- ribosome assembly Source: CACAO
Keywordsi
Molecular function | Helicase, Hydrolase, Initiation factor |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q7Z478 |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent RNA helicase DHX29UniRule annotation (EC:3.6.4.13UniRule annotation)Alternative name(s): DEAH box protein 29UniRule annotation Nucleic acid helicase DDXx |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:15815, DHX29 |
MIMi | 612720, gene |
neXtProti | NX_Q7Z478 |
VEuPathDBi | HostDB:ENSG00000067248.9 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Cytosol
- cytosolic small ribosomal subunit Source: CACAO
Other locations
- eukaryotic 43S preinitiation complex Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 54505 |
OpenTargetsi | ENSG00000067248 |
PharmGKBi | PA27215 |
Miscellaneous databases
Pharosi | Q7Z478, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4105909 |
Genetic variation databases
BioMutai | DHX29 |
DMDMi | 110278938 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000245535 | 1 – 1369 | ATP-dependent RNA helicase DHX29Add BLAST | 1369 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 71 | PhosphoserineCombined sources | 1 | |
Modified residuei | 192 | PhosphoserineCombined sources | 1 | |
Modified residuei | 200 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q7Z478 |
jPOSTi | Q7Z478 |
MassIVEi | Q7Z478 |
MaxQBi | Q7Z478 |
PaxDbi | Q7Z478 |
PeptideAtlasi | Q7Z478 |
PRIDEi | Q7Z478 |
ProteomicsDBi | 69161 |
PTM databases
iPTMneti | Q7Z478 |
PhosphoSitePlusi | Q7Z478 |
Expressioni
Gene expression databases
Bgeei | ENSG00000067248, Expressed in female gonad and 245 other tissues |
ExpressionAtlasi | Q7Z478, baseline and differential |
Genevisiblei | Q7Z478, HS |
Organism-specific databases
HPAi | ENSG00000067248, Low tissue specificity |
Interactioni
Subunit structurei
Part of the 43S pre-initiation complex (PIC) that contains at least Met-tRNA, EIF1, EIF1A (EIF1AX or EIF1AY), EIF2S1, EIF2S2, EIF2S3, EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L, EIF3M, DHX29 and the 40S ribosomal subunit.
UniRule annotation1 PublicationGO - Molecular functioni
- cadherin binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 120001, 55 interactors |
IntActi | Q7Z478, 23 interactors |
MINTi | Q7Z478 |
STRINGi | 9606.ENSP00000251636 |
Chemistry databases
BindingDBi | Q7Z478 |
Miscellaneous databases
RNActi | Q7Z478, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 582 – 755 | Helicase ATP-bindingUniRule annotationAdd BLAST | 174 | |
Domaini | 849 – 1026 | Helicase C-terminalUniRule annotationAdd BLAST | 178 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 222 – 256 | UniRule annotationAdd BLAST | 35 | |
Coiled coili | 283 – 310 | UniRule annotationAdd BLAST | 28 | |
Coiled coili | 492 – 519 | UniRule annotationAdd BLAST | 28 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 702 – 705 | DEAH boxUniRule annotation | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 10 – 53 | Ala-richAdd BLAST | 44 | |
Compositional biasi | 789 – 793 | Poly-Glu | 5 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0920, Eukaryota |
GeneTreei | ENSGT00940000157286 |
HOGENOMi | CLU_001832_1_4_1 |
InParanoidi | Q7Z478 |
OrthoDBi | 278674at2759 |
PhylomeDBi | Q7Z478 |
TreeFami | TF324744 |
Family and domain databases
HAMAPi | MF_03068, DHX29, 1 hit |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR034730, DHX29 IPR002464, DNA/RNA_helicase_DEAH_CS IPR011709, DUF1605 IPR007502, Helicase-assoc_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF04408, HA2, 1 hit PF00271, Helicase_C, 1 hit PF07717, OB_NTP_bind, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00847, HA2, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00690, DEAH_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q7Z478-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGGKNKKHKA PAAAVVRAAV SASRAKSAEA GIAGEAQSKK PVSRPATAAA
60 70 80 90 100
AAAGSREPRV KQGPKIYSFN STNDSSGPAN LDKSILKVVI NNKLEQRIIG
110 120 130 140 150
VINEHKKQNN DKGMISGRLT AKKLQDLYMA LQAFSFKTKD IEDAMTNTLL
160 170 180 190 200
YGGDLHSALD WLCLNLSDDA LPEGFSQEFE EQQPKSRPKF QSPQIQATIS
210 220 230 240 250
PPLQPKTKTY EEDPKSKPKK EEKNMEVNMK EWILRYAEQQ NEEEKNENSK
260 270 280 290 300
SLEEEEKFDP NERYLHLAAK LLDAKEQAAT FKLEKNKQGQ KEAQEKIRKF
310 320 330 340 350
QREMETLEDH PVFNPAMKIS HQQNERKKPP VATEGESALN FNLFEKSAAA
360 370 380 390 400
TEEEKDKKKE PHDVRNFDYT ARSWTGKSPK QFLIDWVRKN LPKSPNPSFE
410 420 430 440 450
KVPVGRYWKC RVRVIKSEDD VLVVCPTILT EDGMQAQHLG ATLALYRLVK
460 470 480 490 500
GQSVHQLLPP TYRDVWLEWS DAEKKREELN KMETNKPRDL FIAKLLNKLK
510 520 530 540 550
QQQQQQQQHS ENKRENSEDP EESWENLVSD EDFSALSLES ANVEDLEPVR
560 570 580 590 600
NLFRKLQSTP KYQKLLKERQ QLPVFKHRDS IVETLKRHRV VVVAGETGSG
610 620 630 640 650
KSTQVPHFLL EDLLLNEWEA SKCNIVCTQP RRISAVSLAN RVCDELGCEN
660 670 680 690 700
GPGGRNSLCG YQIRMESRAC ESTRLLYCTT GVLLRKLQED GLLSNVSHVI
710 720 730 740 750
VDEVHERSVQ SDFLLIILKE ILQKRSDLHL ILMSATVDSE KFSTYFTHCP
760 770 780 790 800
ILRISGRSYP VEVFHLEDII EETGFVLEKD SEYCQKFLEE EEEVTINVTS
810 820 830 840 850
KAGGIKKYQE YIPVQTGAHA DLNPFYQKYS SRTQHAILYM NPHKINLDLI
860 870 880 890 900
LELLAYLDKS PQFRNIEGAV LIFLPGLAHI QQLYDLLSND RRFYSERYKV
910 920 930 940 950
IALHSILSTQ DQAAAFTLPP PGVRKIVLAT NIAETGITIP DVVFVIDTGR
960 970 980 990 1000
TKENKYHESS QMSSLVETFV SKASALQRQG RAGRVRDGFC FRMYTRERFE
1010 1020 1030 1040 1050
GFMDYSVPEI LRVPLEELCL HIMKCNLGSP EDFLSKALDP PQLQVISNAM
1060 1070 1080 1090 1100
NLLRKIGACE LNEPKLTPLG QHLAALPVNV KIGKMLIFGA IFGCLDPVAT
1110 1120 1130 1140 1150
LAAVMTEKSP FTTPIGRKDE ADLAKSALAM ADSDHLTIYN AYLGWKKARQ
1160 1170 1180 1190 1200
EGGYRSEITY CRRNFLNRTS LLTLEDVKQE LIKLVKAAGF SSSTTSTSWE
1210 1220 1230 1240 1250
GNRASQTLSF QEIALLKAVL VAGLYDNVGK IIYTKSVDVT EKLACIVETA
1260 1270 1280 1290 1300
QGKAQVHPSS VNRDLQTHGW LLYQEKIRYA RVYLRETTLI TPFPVLLFGG
1310 1320 1330 1340 1350
DIEVQHRERL LSIDGWIYFQ APVKIAVIFK QLRVLIDSVL RKKLENPKMS
1360
LENDKILQII TELIKTENN
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WYN9 | A0A087WYN9_HUMAN | ATP-dependent RNA helicase DHX29 | DHX29 | 1,370 | Annotation score: | ||
H0Y8L1 | H0Y8L1_HUMAN | ATP-dependent RNA helicase DHX29 | DHX29 | 160 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 121 | A → D in CAH56172 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 185 | K → E in CAH56153 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 356 | D → G in AAK64516 (Ref. 1) Curated | 1 | |
Sequence conflicti | 885 | D → G in CAH56172 (PubMed:17974005).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_052180 | 309 | D → A. Corresponds to variant dbSNP:rs35874395Ensembl. | 1 | |
Natural variantiVAR_026985 | 630 | P → H1 PublicationCorresponds to variant dbSNP:rs17854904Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY036974 mRNA Translation: AAK64516.1 BC056219 mRNA Translation: AAH56219.1 AL834496 mRNA Translation: CAD39154.1 BX648101 mRNA Translation: CAH56172.1 BX648269 mRNA Translation: CAH56153.1 AL079292 mRNA Translation: CAB45191.1 AF070639 mRNA Translation: AAC25394.1 |
CCDSi | CCDS34158.1 |
RefSeqi | NP_001332893.1, NM_001345964.1 NP_001332894.1, NM_001345965.1 NP_061903.2, NM_019030.3 |
Genome annotation databases
Ensembli | ENST00000251636; ENSP00000251636; ENSG00000067248 |
GeneIDi | 54505 |
KEGGi | hsa:54505 |
UCSCi | uc003jpx.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY036974 mRNA Translation: AAK64516.1 BC056219 mRNA Translation: AAH56219.1 AL834496 mRNA Translation: CAD39154.1 BX648101 mRNA Translation: CAH56172.1 BX648269 mRNA Translation: CAH56153.1 AL079292 mRNA Translation: CAB45191.1 AF070639 mRNA Translation: AAC25394.1 |
CCDSi | CCDS34158.1 |
RefSeqi | NP_001332893.1, NM_001345964.1 NP_001332894.1, NM_001345965.1 NP_061903.2, NM_019030.3 |
3D structure databases
SMRi | Q7Z478 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 120001, 55 interactors |
IntActi | Q7Z478, 23 interactors |
MINTi | Q7Z478 |
STRINGi | 9606.ENSP00000251636 |
Chemistry databases
BindingDBi | Q7Z478 |
ChEMBLi | CHEMBL4105909 |
PTM databases
iPTMneti | Q7Z478 |
PhosphoSitePlusi | Q7Z478 |
Genetic variation databases
BioMutai | DHX29 |
DMDMi | 110278938 |
Proteomic databases
EPDi | Q7Z478 |
jPOSTi | Q7Z478 |
MassIVEi | Q7Z478 |
MaxQBi | Q7Z478 |
PaxDbi | Q7Z478 |
PeptideAtlasi | Q7Z478 |
PRIDEi | Q7Z478 |
ProteomicsDBi | 69161 |
Protocols and materials databases
Antibodypediai | 11081, 91 antibodies |
Genome annotation databases
Ensembli | ENST00000251636; ENSP00000251636; ENSG00000067248 |
GeneIDi | 54505 |
KEGGi | hsa:54505 |
UCSCi | uc003jpx.4, human |
Organism-specific databases
CTDi | 54505 |
DisGeNETi | 54505 |
GeneCardsi | DHX29 |
HGNCi | HGNC:15815, DHX29 |
HPAi | ENSG00000067248, Low tissue specificity |
MIMi | 612720, gene |
neXtProti | NX_Q7Z478 |
OpenTargetsi | ENSG00000067248 |
PharmGKBi | PA27215 |
VEuPathDBi | HostDB:ENSG00000067248.9 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0920, Eukaryota |
GeneTreei | ENSGT00940000157286 |
HOGENOMi | CLU_001832_1_4_1 |
InParanoidi | Q7Z478 |
OrthoDBi | 278674at2759 |
PhylomeDBi | Q7Z478 |
TreeFami | TF324744 |
Enzyme and pathway databases
PathwayCommonsi | Q7Z478 |
Miscellaneous databases
BioGRID-ORCSi | 54505, 104 hits in 1009 CRISPR screens |
ChiTaRSi | DHX29, human |
GenomeRNAii | 54505 |
Pharosi | Q7Z478, Tbio |
PROi | PR:Q7Z478 |
RNActi | Q7Z478, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000067248, Expressed in female gonad and 245 other tissues |
ExpressionAtlasi | Q7Z478, baseline and differential |
Genevisiblei | Q7Z478, HS |
Family and domain databases
HAMAPi | MF_03068, DHX29, 1 hit |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR034730, DHX29 IPR002464, DNA/RNA_helicase_DEAH_CS IPR011709, DUF1605 IPR007502, Helicase-assoc_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF04408, HA2, 1 hit PF00271, Helicase_C, 1 hit PF07717, OB_NTP_bind, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00847, HA2, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00690, DEAH_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DHX29_HUMAN | |
Accessioni | Q7Z478Primary (citable) accession number: Q7Z478 Secondary accession number(s): O75549 Q9UMH2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2006 |
Last sequence update: | July 11, 2006 | |
Last modified: | April 7, 2021 | |
This is version 163 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 5
Human chromosome 5: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families