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Protein

E3 ubiquitin-protein ligase RNF144B

Gene

RNF144B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.2 Publications

Caution

Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.Curated

Catalytic activityi

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei206By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri30 – 80RING-type 1; atypicalAdd BLAST51
Zinc fingeri101 – 166IBR-typeAdd BLAST66
Zinc fingeri193 – 222RING-type 2; degenerateAdd BLAST30

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processApoptosis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF144B (EC:2.3.2.31By similarity)
Alternative name(s):
IBR domain-containing protein 2
RING finger protein 144B
p53-inducible RING finger protein
Gene namesi
Name:RNF144B
Synonyms:IBRDC2, P53RFP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000137393.9
HGNCiHGNC:21578 RNF144B
neXtProtiNX_Q7Z419

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei258 – 278HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi255488
OpenTargetsiENSG00000137393
PharmGKBiPA162401565

Polymorphism and mutation databases

BioMutaiRNF144B
DMDMi57012811

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000559111 – 303E3 ubiquitin-protein ligase RNF144BAdd BLAST303

Post-translational modificationi

Auto-ubiquitinated.

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ7Z419
PeptideAtlasiQ7Z419
PRIDEiQ7Z419
ProteomicsDBi69130

PTM databases

iPTMnetiQ7Z419
PhosphoSitePlusiQ7Z419

Expressioni

Tissue specificityi

Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.2 Publications

Gene expression databases

BgeeiENSG00000137393 Expressed in 203 organ(s), highest expression level in quadriceps femoris
CleanExiHS_RNF144B
GenevisibleiQ7Z419 HS

Organism-specific databases

HPAiCAB046013
HPA054127
HPA072469

Interactioni

Subunit structurei

Interacts with UBE2L3, UBE2L6 and LCMT2, as well as with BAX.3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi129106, 16 interactors
DIPiDIP-43771N
IntActiQ7Z419, 11 interactors
MINTiQ7Z419
STRINGi9606.ENSP00000259939

Structurei

3D structure databases

ProteinModelPortaliQ7Z419
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme (By similarity). The transmembrane domain is essential for translocation to the mitochondria upon induction of apoptosis.By similarity
Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.By similarity

Sequence similaritiesi

Belongs to the RBR family. RNF144 subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri30 – 80RING-type 1; atypicalAdd BLAST51
Zinc fingeri101 – 166IBR-typeAdd BLAST66
Zinc fingeri193 – 222RING-type 2; degenerateAdd BLAST30

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1815 Eukaryota
ENOG410XP9Y LUCA
GeneTreeiENSGT00840000129738
HOGENOMiHOG000007696
HOVERGENiHBG052072
InParanoidiQ7Z419
KOiK11975
OMAiVCRIYIE
OrthoDBiEOG091G0FQQ
PhylomeDBiQ7Z419
TreeFamiTF324777

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR031127 E3_UB_ligase_RBR
IPR002867 IBR_dom
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR11685 PTHR11685, 1 hit
PfamiView protein in Pfam
PF01485 IBR, 2 hits
SMARTiView protein in SMART
SM00647 IBR, 2 hits
SM00184 RING, 2 hits
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q7Z419-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGSAGRLHYL AMTAENPTPG DLAPAPLITC KLCLCEQSLD KMTTLQECQC
60 70 80 90 100
IFCTACLKQY MQLAIREGCG SPITCPDMVC LNHGTLQEAE IACLVPVDQF
110 120 130 140 150
QLYQRLKFER EVHLDPYRTW CPVADCQTVC PVASSDPGQP VLVECPSCHL
160 170 180 190 200
KFCSCCKDAW HAEVSCRDSQ PIVLPTEHRA LFGTDAEAPI KQCPVCRVYI
210 220 230 240 250
ERNEGCAQMM CKNCKHTFCW YCLQNLDNDI FLRHYDKGPC RNKLGHSRAS
260 270 280 290 300
VMWNRTQVVG ILVGLGIIAL VTSPLLLLAS PCIICCVCKS CRGKKKKHDP

STT
Length:303
Mass (Da):33,697
Last modified:October 1, 2003 - v1
Checksum:i9FD505CDFB70B6A5
GO
Isoform 2 (identifier: Q7Z419-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: MGSAGRLHYLAMTAENPTPGD → MLWITMPHPAHLGLFIAVTLS
     22-110: Missing.

Note: No experimental confirmation available.
Show »
Length:214
Mass (Da):23,856
Checksum:i79776522FF903E30
GO

Sequence cautioni

The sequence CAD38622 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti262L → P in CAD38622 (PubMed:17974005).Curated1
Sequence conflicti275Missing in AAH63311 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0558531 – 21MGSAG…PTPGD → MLWITMPHPAHLGLFIAVTL S in isoform 2. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_05585422 – 110Missing in isoform 2. 1 PublicationAdd BLAST89

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB076367 mRNA Translation: BAC02434.1
AK096832 mRNA Translation: BAG53370.1
AK302935 mRNA Translation: BAG64094.1
AL832329 mRNA Translation: CAD38622.1 Different initiation.
AL138725 Genomic DNA No translation available.
CH471087 Genomic DNA Translation: EAW55407.1
BC063311 mRNA Translation: AAH63311.1
CCDSiCCDS34345.1 [Q7Z419-1]
RefSeqiNP_877434.2, NM_182757.3 [Q7Z419-1]
UniGeneiHs.148741

Genome annotation databases

EnsembliENST00000259939; ENSP00000259939; ENSG00000137393 [Q7Z419-1]
GeneIDi255488
KEGGihsa:255488
UCSCiuc003ncs.3 human [Q7Z419-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiR144B_HUMAN
AccessioniPrimary (citable) accession number: Q7Z419
Secondary accession number(s): B3KUA8
, B4DZI2, Q5TB85, Q6P4Q0, Q8N3R7, Q9BX38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 1, 2003
Last modified: September 12, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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