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Protein

Chitinase-3-like protein 1

Gene

CHI3L1

Organism
Bubalus bubalis (Domestic water buffalo)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung (By similarity).By similarity

Caution

Although it belongs to the glycosyl hydrolase 18 family, Leu-140 is present instead of the conserved Glu which is an active site residue. Therefore this protein lacks chitinase activity.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei263ChitooligosaccharideBy similarity1
Binding sitei352ChitooligosaccharideBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntimicrobial
Biological processApoptosis, Inflammatory response
LigandLectin

Protein family/group databases

CAZyiGH18 Glycoside Hydrolase Family 18

Names & Taxonomyi

Protein namesi
Recommended name:
Chitinase-3-like protein 1
Alternative name(s):
Mammary gland protein 40
SPB-40
Gene namesi
Name:CHI3L1
Synonyms:MGP-40
OrganismiBubalus bubalis (Domestic water buffalo)
Taxonomic identifieri89462 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBubalus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000007705422 – 383Chitinase-3-like protein 1Add BLAST362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 511 Publication
Glycosylationi60N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi300 ↔ 3641 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ7YS85

PTM databases

iPTMnetiQ7YS85

Expressioni

Tissue specificityi

Detected in mammary gland.

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1383
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SMRiQ7YS85
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7YS85

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 71Chitooligosaccharide bindingBy similarity2
Regioni97 – 100Chitooligosaccharide bindingBy similarity4
Regioni204 – 207Chitooligosaccharide binding4
Regioni324 – 338Important for AKT1 activation and IL8 productionBy similarityAdd BLAST15

Sequence similaritiesi

Belongs to the glycosyl hydrolase 18 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG011684
OrthoDBiEOG091G014W

Family and domain databases

Gene3Di3.10.50.10, 1 hit
InterProiView protein in InterPro
IPR028538 CHI3L1
IPR011583 Chitinase_II
IPR029070 Chitinase_insertion_sf
IPR001223 Glyco_hydro18_cat
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR11177:SF202 PTHR11177:SF202, 1 hit
PfamiView protein in Pfam
PF00704 Glyco_hydro_18, 1 hit
SMARTiView protein in SMART
SM00636 Glyco_18, 1 hit
SUPFAMiSSF51445 SSF51445, 2 hits
SSF54556 SSF54556, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7YS85-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGLRVAQTGF VVLVLLQSCA AYKLICYYTS WSQYREGDGS CFPDAIDPFL
60 70 80 90 100
CTHVIYSFAN ISNNEIDTWE WNDVTLYDTL NTLKNRNPKL KTLLSVGGWN
110 120 130 140 150
FGSQRFSKIA SKTQSRRTFI KSVPPFLRTH GFDGLDLAWL YPGWRDKRHL
160 170 180 190 200
TTLVKEMKAE FVREAQAGTE QLLLSAAVPA GKIAIDRGYD IAQISRHLDF
210 220 230 240 250
ISLLTYDFHG AWRQTVGHHS PLFRGQEDAS SDRFSNADYA VSYMLRLGAP
260 270 280 290 300
ANKLVMGIPT FGKSYTLASS KTDVGAPISG PGIPGQFTKE KGILAYYEIC
310 320 330 340 350
DFLHGATTHR FRDQQVPYAT KGNQWVAYDD QESVKNKARY LKNRQLAGAM
360 370 380
VWALDLDDFR GTFCGQNLAF PLTNAIKDVL AGV
Length:383
Mass (Da):42,935
Last modified:May 14, 2014 - v3
Checksum:i8FE18A55F586AF4D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89K → N in AAP42568 (Ref. 1) Curated1
Sequence conflicti101F → Y in AAP42568 (Ref. 1) Curated1
Sequence conflicti141Y → W in AAP42568 (Ref. 1) Curated1
Sequence conflicti179P → T in AAP42568 (Ref. 1) Curated1
Sequence conflicti226Q → N in AAP42568 (Ref. 1) Curated1
Sequence conflicti232Missing in AAP42568 (Ref. 1) Curated1
Sequence conflicti263K → R in AAP42568 (Ref. 1) Curated1
Sequence conflicti286Q → R in AAP42568 (Ref. 1) Curated1
Sequence conflicti290E → W in AAP42568 (Ref. 1) Curated1
Sequence conflicti369A → T in AAP42568 (Ref. 1) Curated1
Sequence conflicti374N → S in AAP42568 (Ref. 1) Curated1
Sequence conflicti382G → R in AAP42568 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY295929 mRNA Translation: AAP42568.2
JQ796280 mRNA Translation: AFN52415.1
RefSeqiNP_001277897.1, NM_001290968.1

Genome annotation databases

GeneIDi102399197

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY295929 mRNA Translation: AAP42568.2
JQ796280 mRNA Translation: AFN52415.1
RefSeqiNP_001277897.1, NM_001290968.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TFVX-ray2.90A22-381[»]
2O9OX-ray2.80A22-381[»]
2QF8X-ray2.80A22-381[»]
4MAVX-ray2.79A22-381[»]
4ML4X-ray2.50A22-381[»]
4MPKX-ray2.65A22-381[»]
4MTVX-ray2.80A22-381[»]
4NSBX-ray3.05A22-381[»]
4Q7NX-ray1.79A22-381[»]
5Z05X-ray1.49A22-383[»]
5Z3SX-ray1.65A22-383[»]
5Z4WX-ray1.79A22-383[»]
SMRiQ7YS85
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH18 Glycoside Hydrolase Family 18

PTM databases

iPTMnetiQ7YS85

Proteomic databases

PRIDEiQ7YS85

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi102399197

Organism-specific databases

CTDi1116

Phylogenomic databases

HOVERGENiHBG011684
OrthoDBiEOG091G014W

Miscellaneous databases

EvolutionaryTraceiQ7YS85

Family and domain databases

Gene3Di3.10.50.10, 1 hit
InterProiView protein in InterPro
IPR028538 CHI3L1
IPR011583 Chitinase_II
IPR029070 Chitinase_insertion_sf
IPR001223 Glyco_hydro18_cat
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR11177:SF202 PTHR11177:SF202, 1 hit
PfamiView protein in Pfam
PF00704 Glyco_hydro_18, 1 hit
SMARTiView protein in SMART
SM00636 Glyco_18, 1 hit
SUPFAMiSSF51445 SSF51445, 2 hits
SSF54556 SSF54556, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCH3L1_BUBBU
AccessioniPrimary (citable) accession number: Q7YS85
Secondary accession number(s): I6YIV5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: May 14, 2014
Last modified: May 23, 2018
This is version 82 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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