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Protein

Thiamine thiazole synthase, chloroplastic

Gene

THI1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Unreviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei96Substrate; via amide nitrogenUniRule annotation1
Binding sitei124Substrate; via amide nitrogenUniRule annotation1
Binding sitei189Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei220SubstrateUniRule annotation1
Binding sitei235SubstrateUniRule annotation1
Binding sitei287Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processThiamine biosynthesisUniRule annotation
LigandIronUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthase, chloroplasticUniRule annotation
Alternative name(s):
Thiazole biosynthetic enzymeUniRule annotation
Gene namesi
Name:THI1UniRule annotation
Ordered Locus Names:Os07g0529600Imported
ORF Names:OSNPB_070529600Imported
OrganismiOryza sativa subsp. japonica (Rice)Imported
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

ChloroplastUniRule annotation, Plastid

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2182,3-didehydroalanine (Cys)UniRule annotation1

Post-translational modificationi

During the catalytic reaction, a sulfide is transferred from Cys-218 to a reaction intermediate, generating a dehydroalanine residue.UniRule annotation

Expressioni

Gene expression databases

ExpressionAtlasiQ7XXS4 differential

Interactioni

Subunit structurei

Homooctamer.UniRule annotation

Protein-protein interaction databases

STRINGi39947.LOC_Os07g34570.1

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni116 – 117Substrate bindingUniRule annotation2
Regioni297 – 299Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2960 Eukaryota
COG1635 LUCA
HOGENOMiHOG000106048
KOiK03146
OMAiMWGGGMM
OrthoDBiEOG09360HDE

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

Q7XXS4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAMATTASS LLKTSFAGAR LPAAARNPTV SVAPRTGGAI CNSISSSSST
60 70 80 90 100
PPYDLNAIRF SPIKESIVSR EMTRRYMTDM ITYADTDVVV VGAGSAGLSC
110 120 130 140 150
AYELSKDPSV SVAVIEQSVS PGGGAWLGGQ LFSAMVVRKP AHLFLDELGV
160 170 180 190 200
AYDEQEDYVV IKHAALFTST VMSRLLARPN VKLFNAVAVE DLIVKEGRVG
210 220 230 240 250
GVVTNWALVS MNHDTQSCMD PNVMESRVVV SSCGHDGPFG ATGVKRLQDI
260 270 280 290 300
GMIDAVPGMR ALDMNTAEDE IVRLTREVVP GMIVTGMEVA EIDGAPRMGP
310 320 330 340 350
TFGAMMISGQ KAAHLALKAL GRPNAIDGTI KKAAAAAAHP ELILASKDDG

EIVDA
Length:355
Mass (Da):37,225
Last modified:October 1, 2003 - v1
Checksum:iBC5A9DB2221F9FE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB110170 mRNA Translation: BAC78562.1
AP008213 Genomic DNA Translation: BAF21755.1
AK099918 mRNA Translation: BAG94352.1
AP014963 Genomic DNA Translation: BAT01865.1
RefSeqiXP_015647396.1, XM_015791910.1
UniGeneiOs.12767

Genome annotation databases

EnsemblPlantsiOs07t0529600-01; Os07t0529600-01; Os07g0529600
GeneIDi4343443
GrameneiOs07t0529600-01; Os07t0529600-01; Os07g0529600
KEGGiosa:4343443

Similar proteinsi

Entry informationi

Entry nameiQ7XXS4_ORYSJ
AccessioniPrimary (citable) accession number: Q7XXS4
Entry historyiIntegrated into UniProtKB/TrEMBL: October 1, 2003
Last sequence update: October 1, 2003
Last modified: May 23, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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