UniProtKB - Q7XJR9 (CDPKG_ARATH)
Protein
Calcium-dependent protein kinase 16
Gene
CPK16
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
May play a role in signal transduction pathways that involve calcium as a second messenger.
Catalytic activityi
Activity regulationi
Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 137 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 234 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 114 – 122 | ATPPROSITE-ProRule annotation | 9 | |
Calcium bindingi | 424 – 435 | 1PROSITE-ProRule annotationAdd BLAST | 12 | |
Calcium bindingi | 461 – 472 | 2PROSITE-ProRule annotationAdd BLAST | 12 | |
Calcium bindingi | 503 – 514 | 3PROSITE-ProRule annotationAdd BLAST | 12 | |
Calcium bindingi | 533 – 544 | 4PROSITE-ProRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- calcium-dependent protein serine/threonine kinase activity Source: GO_Central
- calcium ion binding Source: InterPro
- calmodulin binding Source: GO_Central
- calmodulin-dependent protein kinase activity Source: GO_Central
- protein kinase activity Source: TAIR
- protein serine kinase activity Source: UniProtKB-EC
- protein threonine kinase activity Source: UniProtKB-EC
GO - Biological processi
- intracellular signal transduction Source: GO_Central
- peptidyl-serine phosphorylation Source: GO_Central
- protein autophosphorylation Source: GO_Central
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Calcium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CPK16 Ordered Locus Names:At2g17890 ORF Names:T13L16.9 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G17890 |
TAIRi | locus:2827826, AT2G17890 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Plasma membrane
- Cell membrane 2 Publications; Lipid-anchor 1 Publication1 Publication
Note: Predominantly localized at the plasma membrane.1 Publication
Nucleus
- nucleus Source: GO_Central
Plasma Membrane
- plasma membrane Source: TAIR
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cell membrane, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | G → A: Loss of myristoylation. Loss of plasma membrane localization and relocation to nucleolus and chloroplasts. 1 Publication | 1 | |
Mutagenesisi | 4 | C → A: Drastic reduction of plasma membrane localization and strong increase of nuclear localization. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCurated | |||
ChainiPRO_0000363338 | 2 – 571 | Calcium-dependent protein kinase 16Add BLAST | 570 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine1 Publication | 1 | |
Lipidationi | 4 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 274 | PhosphoserineBy similarity | 1 | |
Modified residuei | 541 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Lipoprotein, Myristate, Palmitate, PhosphoproteinProteomic databases
PaxDbi | Q7XJR9 |
PRIDEi | Q7XJR9 |
ProteomicsDBi | 224390 |
PTM databases
iPTMneti | Q7XJR9 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q7XJR9, baseline and differential |
Genevisiblei | Q7XJR9, AT |
Interactioni
GO - Molecular functioni
- calmodulin binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 1656, 1 interactor |
STRINGi | 3702.AT2G17890.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 108 – 368 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 261 | |
Domaini | 411 – 447 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 448 – 483 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 484 – 525 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 42 | |
Domaini | 526 – 555 | EF-hand 4PROSITE-ProRule annotationAdd BLAST | 30 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 374 – 404 | Autoinhibitory domainBy similarityAdd BLAST | 31 |
Domaini
There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (374-404) inactivates kinase activity under calcium-free conditions (By similarity).By similarity
Sequence similaritiesi
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0032, Eukaryota |
HOGENOMi | CLU_000288_37_3_1 |
InParanoidi | Q7XJR9 |
OMAi | DHRYTIG |
OrthoDBi | 330091at2759 |
PhylomeDBi | Q7XJR9 |
Family and domain databases
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF13499, EF-hand_7, 2 hits PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 4 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 4 hits PS50222, EF_HAND_2, 4 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q7XJR9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGLCFSSAAK SSGHNRSSRN PHPHPPLTVV KSRPPRSPCS FMAVTIQKDH
60 70 80 90 100
RTQPRRNATA KKTPTRHTPP HGKVREKVIS NNGRRHGETI PYGKRVDFGY
110 120 130 140 150
AKDFDHRYTI GKLLGHGQFG YTYVATDKKT GDRVAVKKID KAKMTIPIAV
160 170 180 190 200
EDVKREVKIL QALTGHENVV RFYNAFEDKN SVYIVMELCE GGELLDRILA
210 220 230 240 250
RKDSRYSERD AAVVVRQMLK VAAECHLRGL VHRDMKPENF LFKSTEEDSP
260 270 280 290 300
LKATDFGLSD FIKPGKKFHD IVGSAYYVAP EVLKRRSGPE SDVWSIGVIS
310 320 330 340 350
YILLCGRRPF WDKTEDGIFK EVLKNKPDFR RKPWPTISNS AKDFVKKLLV
360 370 380 390 400
KDPRARLTAA QALSHPWVRE GGDASEIPID ISVLNNMRQF VKFSRLKQFA
410 420 430 440 450
LRALATTLDE EELADLRDQF DAIDVDKNGV ISLEEMRQAL AKDHPWKLKD
460 470 480 490 500
ARVAEILQAI DSNTDGFVDF GEFVAAALHV NQLEEHDSEK WQQRSRAAFE
510 520 530 540 550
KFDIDGDGFI TAEELRMHTG LKGSIEPLLE EADIDNDGKI SLQEFRRLLR
560 570
TASIKSRNVR SPPGYLISRK V
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP002685 Genomic DNA Translation: AEC06699.1 |
PIRi | T00835 |
RefSeqi | NP_179379.1, NM_127343.2 |
Genome annotation databases
EnsemblPlantsi | AT2G17890.1; AT2G17890.1; AT2G17890 |
GeneIDi | 816299 |
Gramenei | AT2G17890.1; AT2G17890.1; AT2G17890 |
KEGGi | ath:AT2G17890 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP002685 Genomic DNA Translation: AEC06699.1 |
PIRi | T00835 |
RefSeqi | NP_179379.1, NM_127343.2 |
3D structure databases
SMRi | Q7XJR9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 1656, 1 interactor |
STRINGi | 3702.AT2G17890.1 |
PTM databases
iPTMneti | Q7XJR9 |
Proteomic databases
PaxDbi | Q7XJR9 |
PRIDEi | Q7XJR9 |
ProteomicsDBi | 224390 |
Genome annotation databases
EnsemblPlantsi | AT2G17890.1; AT2G17890.1; AT2G17890 |
GeneIDi | 816299 |
Gramenei | AT2G17890.1; AT2G17890.1; AT2G17890 |
KEGGi | ath:AT2G17890 |
Organism-specific databases
Araporti | AT2G17890 |
TAIRi | locus:2827826, AT2G17890 |
Phylogenomic databases
eggNOGi | KOG0032, Eukaryota |
HOGENOMi | CLU_000288_37_3_1 |
InParanoidi | Q7XJR9 |
OMAi | DHRYTIG |
OrthoDBi | 330091at2759 |
PhylomeDBi | Q7XJR9 |
Miscellaneous databases
PROi | PR:Q7XJR9 |
Gene expression databases
ExpressionAtlasi | Q7XJR9, baseline and differential |
Genevisiblei | Q7XJR9, AT |
Family and domain databases
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF13499, EF-hand_7, 2 hits PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 4 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 4 hits PS50222, EF_HAND_2, 4 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CDPKG_ARATH | |
Accessioni | Q7XJR9Primary (citable) accession number: Q7XJR9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 10, 2009 |
Last sequence update: | October 1, 2003 | |
Last modified: | April 7, 2021 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families