UniProtKB - Q7XD96 (DCL3B_ORYSJ)
Protein
Endoribonuclease Dicer homolog 3b
Gene
DCL3B
Organism
Oryza sativa subsp. japonica (Rice)
Status
Functioni
Probably involved in the RNA silencing pathway. May cleave double-stranded RNA to produce short 21-24 nucleotides (nt) RNAs which target the selective destruction of complementary RNAs (By similarity).By similarity
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 1214 | Magnesium or manganeseBy similarity | 1 | |
Sitei | 1305 | Important for activityBy similarity | 1 | |
Metal bindingi | 1309 | Magnesium or manganeseBy similarity | 1 | |
Metal bindingi | 1312 | Magnesium or manganeseBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 59 – 66 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- helicase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- ribonuclease III activity Source: GO_Central
- RNA binding Source: GO_Central
GO - Biological processi
- production of siRNA involved in RNA interference Source: GO_Central
Keywordsi
Molecular function | Endonuclease, Helicase, Hydrolase, Nuclease, RNA-binding |
Biological process | RNA-mediated gene silencing |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Endoribonuclease Dicer homolog 3bAlternative name(s): |
Gene namesi | Name:DCL3B Ordered Locus Names:Os10g0485600, LOC_Os10g34430 ORF Names:OSJNBa0029C15.23 |
Organismi | Oryza sativa subsp. japonica (Rice) |
Taxonomic identifieri | 39947 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000378420 | 1 – 1637 | Endoribonuclease Dicer homolog 3bAdd BLAST | 1637 |
Proteomic databases
PaxDbi | Q7XD96 |
PRIDEi | Q7XD96 |
Interactioni
Subunit structurei
May interact with ARGONAUTE1 or PINHEAD through their common PAZ domains.
By similarityProtein-protein interaction databases
STRINGi | 4530.OS10T0485600-00 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 46 – 222 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 177 | |
Domaini | 404 – 556 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 153 | |
Domaini | 581 – 671 | Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd BLAST | 91 | |
Domaini | 867 – 1006 | PAZPROSITE-ProRule annotationAdd BLAST | 140 | |
Domaini | 1031 – 1200 | RNase III 1PROSITE-ProRule annotationAdd BLAST | 170 | |
Domaini | 1241 – 1389 | RNase III 2PROSITE-ProRule annotationAdd BLAST | 149 | |
Domaini | 1412 – 1481 | DRBM 1Add BLAST | 70 | |
Domaini | 1545 – 1629 | DRBM 2Add BLAST | 85 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 169 – 172 | DECH box | 4 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0701, Eukaryota |
HOGENOMi | CLU_000907_4_4_1 |
InParanoidi | Q7XD96 |
Family and domain databases
CDDi | cd00593, RIBOc, 2 hits |
Gene3Di | 1.10.1520.10, 1 hit 3.30.160.380, 1 hit |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR038248, Dicer_dimer_sf IPR005034, Dicer_dimerisation_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR003100, PAZ_dom IPR036085, PAZ_dom_sf IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit PF02170, PAZ, 1 hit PF00636, Ribonuclease_3, 2 hits |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00949, PAZ, 1 hit SM00535, RIBOc, 2 hits |
SUPFAMi | SSF101690, SSF101690, 1 hit SSF52540, SSF52540, 1 hit SSF69065, SSF69065, 2 hits |
PROSITEi | View protein in PROSITE PS51327, DICER_DSRBF, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS50821, PAZ, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q7XD96-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADDEAAVLP PPPPLPPPCR PHRQLRPRGS RPTADTTPRT SQLVEVFEAA
60 70 80 90 100
LRGNTIAVLD TGSGKTMVAV MLAREHARRV RAGEAPRRIV VFLAPTVHLV
110 120 130 140 150
HQQFEVIREY TDLDVMMCSG ASRVGEWGAD HWKEEVGRNE IVVMTPQILL
160 170 180 190 200
DALRHAFLTM SAVSLLIFDE CHRACGSHPY ARIMKEFYFG SQWRPDVFGM
210 220 230 240 250
TASPVATKGA STLHNCEAHI SQLELTLDAK IYIVEDRNEL ESFSPPTTIV
260 270 280 290 300
NKYYDAYMVD FDNLKSKLQI FSDEFDSLLV GLQESPSNKF KDTDNILETS
310 320 330 340 350
RKSLSRYHGK ILYSLNDLGP IITSEVVKIH IESVKPLCDS EDCIFSKASL
360 370 380 390 400
CLHMSYFKEA LSLIEEILPQ GYGELMKSES GSEELTKRGY ISSKVNTLIN
410 420 430 440 450
IFKSFGSSNE VLCLIFVDRI ITAKAVERFM RGIVNFSCFS ISYLTGGSTS
460 470 480 490 500
KDALSPAVQR FTLDLFRAGK VNLLFTTDVT EEGVDVPNCS CVIRFDLPRT
510 520 530 540 550
VCSYVQSRGR ARRNNSEFIL MIERGNLQQQ EHIFRMIQTG YYVKNCALYR
560 570 580 590 600
HPNALSYDLS IQGMYTYQVQ STGATITADC CVNLIRKYCE KLPKDRYFMP
610 620 630 640 650
KPSFEVTIED GLFKCTLTLP RNAAFQSIVG PLSSSSNLSK QLVSLEACKK
660 670 680 690 700
LHQLGELNDH LVPLTEEPMD TDFTTADEKC ISGPGTTKRK ELHGTTCVLA
710 720 730 740 750
LSGTWIHDSE NITLNTYRID FLCDQEGENY AGFVLLMEPE LDDDVAPSKM
760 770 780 790 800
DLFLIPNKMV YTTVTPRGKV QLNKKQLGKG KLFQEFFFNG IFGRLFHGSR
810 820 830 840 850
KSGAQRDFIF KKGHEIQWNT ESMYLLLPLR DSSYIQDDLS IHWEAIESCA
860 870 880 890 900
GAVEQLWSSY QGDENVIPVN CIPQKRRGGQ EEIIHLANKS LHCSSIKDSV
910 920 930 940 950
VLSLHTGRIY TVLDLILDTT AEDSFDEMCK GKASPFTSFV DYYHQKYGII
960 970 980 990 1000
IQHPEQPLLL LKQSHNAHNL LFSKLKYLDG STGKPLLMEK EQIHARVPPE
1010 1020 1030 1040 1050
LLIHLDVTTD ILKSFYLLPS VIHRLQSLML ASQLRREIGY NQHIPVTLIL
1060 1070 1080 1090 1100
EAITTLRCCE TFSLERLELL GDSVLKYVVG CDLFLRYPMK HEGQLSDMRS
1110 1120 1130 1140 1150
KAVCNATLHK HGIWRSLQGY VRDNAFDPRR WVAPGQISLR PFPCNCGIET
1160 1170 1180 1190 1200
AFVPSHRRYI RDDPSFFVGK PCDRGHRWMC SKTISDCVEA LVGAYYVGGG
1210 1220 1230 1240 1250
IAAALWVMRW FGIDIKCDMK LLQEVKFNAS HLCSLSKIND IEELEAKLKY
1260 1270 1280 1290 1300
NFSVKGLLLE AITHPSLQEL GVDYCYQRLE FLGDSVLDLL LTRHLYATHT
1310 1320 1330 1340 1350
DVDPGELTDL RSALVSNENF AQAVVRNNIH SHLQHGSGIL LEQITEYVRS
1360 1370 1380 1390 1400
NLECQGKESE FLQHTTCKVP KVLGDIMESI AGAVFIDTDF NVDMVWEIFE
1410 1420 1430 1440 1450
PLLSPLITPD KLALPPYREL LELCSHIGCF LNSKCTSKGE EVIIEMSLQL
1460 1470 1480 1490 1500
RDELLVAQGH DRNKKRAKAK AASRILADLK QQGLSIKQCL SKAKQLDIVT
1510 1520 1530 1540 1550
SDLQFDLTSS GTQLSYSDLN DYHILEGLSS VKKEVVLPLK MEKGGPRSAL
1560 1570 1580 1590 1600
FKLCKILQWP MPEFEFVEQR FRTPIVMDGA TTTNFNSFVS TITLHIPDAT
1610 1620 1630
TITFQGERRT DKKSAQDSAS LMMLHKLQEL KICICKT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0P0XVZ5 | A0A0P0XVZ5_ORYSJ | Os10g0485600 protein | Os10g0485600, OSNPB_100485600 | 1,613 | Annotation score: |
Sequence cautioni
The sequence AAL59041 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAP54346 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAF26812 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC087182 Genomic DNA Translation: AAL59041.1 Sequence problems. DP000086 Genomic DNA Translation: AAP54346.1 Sequence problems. AP008216 Genomic DNA Translation: BAF26812.2 Sequence problems. AP014966 Genomic DNA No translation available. |
Genome annotation databases
EnsemblPlantsi | Os10t0485600-01; Os10t0485600-01; Os10g0485600 |
Gramenei | Os10t0485600-01; Os10t0485600-01; Os10g0485600 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC087182 Genomic DNA Translation: AAL59041.1 Sequence problems. DP000086 Genomic DNA Translation: AAP54346.1 Sequence problems. AP008216 Genomic DNA Translation: BAF26812.2 Sequence problems. AP014966 Genomic DNA No translation available. |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 4530.OS10T0485600-00 |
Proteomic databases
PaxDbi | Q7XD96 |
PRIDEi | Q7XD96 |
Genome annotation databases
EnsemblPlantsi | Os10t0485600-01; Os10t0485600-01; Os10g0485600 |
Gramenei | Os10t0485600-01; Os10t0485600-01; Os10g0485600 |
Phylogenomic databases
eggNOGi | KOG0701, Eukaryota |
HOGENOMi | CLU_000907_4_4_1 |
InParanoidi | Q7XD96 |
Gene expression databases
Genevisiblei | Q7XD96, OS |
Family and domain databases
CDDi | cd00593, RIBOc, 2 hits |
Gene3Di | 1.10.1520.10, 1 hit 3.30.160.380, 1 hit |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR038248, Dicer_dimer_sf IPR005034, Dicer_dimerisation_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR003100, PAZ_dom IPR036085, PAZ_dom_sf IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit PF02170, PAZ, 1 hit PF00636, Ribonuclease_3, 2 hits |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00949, PAZ, 1 hit SM00535, RIBOc, 2 hits |
SUPFAMi | SSF101690, SSF101690, 1 hit SSF52540, SSF52540, 1 hit SSF69065, SSF69065, 2 hits |
PROSITEi | View protein in PROSITE PS51327, DICER_DSRBF, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS50821, PAZ, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DCL3B_ORYSJ | |
Accessioni | Q7XD96Primary (citable) accession number: Q7XD96 Secondary accession number(s): Q0IWV3, Q8W367 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 7, 2009 |
Last sequence update: | July 7, 2009 | |
Last modified: | December 2, 2020 | |
This is version 110 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Oryza sativa (rice)
Index of Oryza sativa entries and their corresponding gene designations - SIMILARITY comments
Index of protein domains and families