UniProtKB - Q7WY62 (SDA_BACSU)
Sporulation inhibitor sda
sda
Functioni
Mediates a developmental checkpoint inhibiting initiation of sporulation (by preventing phosphorylation of spo0A) in response to defects in the replication initiation machinery. Inhibits autophosphorylation of the histidine protein kinase KinA, forming a molecular barricade that prevents productive interaction between the ATP-binding site in the catalytic domain and the phosphorylatable His in the phosphotransfer domain of KinA. Probably also inhibits the activity of KinB, but has relatively little effect on KinC. Has at least one target in vivo in addition to KinA as sda does not require KinA to inhibit sporulation.
2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 25 | Important for interaction with KinACurated | 1 | |
Sitei | 27 | Important for interaction with KinACurated | 1 | |
Sitei | 31 | Important for interaction with KinACurated | 1 |
GO - Molecular functioni
- protein kinase inhibitor activity Source: UniProtKB-KW
GO - Biological processi
- sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Keywordsi
Molecular function | Protein kinase inhibitor |
Biological process | Sporulation |
Enzyme and pathway databases
BioCyci | BSUB:BSU25690-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Sporulation inhibitor sdaAlternative name(s): Histidine kinase KinA inhibitor |
Gene namesi | Name:sda Ordered Locus Names:BSU25690 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 25 | M → A or I: Partial loss of ability to inhibit KinA autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 27 | L → A or E: Complete loss of ability to inhibit KinA autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 31 | F → H: Complete loss of ability to inhibit KinA autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 34 | L → A: Partial loss of ability to inhibit KinA autophosphorylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000022288 | 1 – 52 | Sporulation inhibitor sdaAdd BLAST | 52 |
Proteomic databases
PaxDbi | Q7WY62 |
PRIDEi | Q7WY62 |
Interactioni
Subunit structurei
Forms a stable heterotetramer with KinA (comprising two molecules of each protein), by binding to the KinA dimerization/phosphotransfer domain.
Binary interactionsi
Q7WY62
With | #Exp. | IntAct |
---|---|---|
kinA [P16497] | 6 | EBI-6405714,EBI-6405707 |
Protein-protein interaction databases
IntActi | Q7WY62, 1 interactor |
STRINGi | 224308.BSU25690 |
Structurei
Secondary structure
3D structure databases
BMRBi | Q7WY62 |
SASBDBi | Q7WY62 |
SMRi | Q7WY62 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q7WY62 |
Family & Domainsi
Phylogenomic databases
eggNOGi | ENOG5033C8I, Bacteria |
OMAi | MKCLPDD |
Family and domain databases
Gene3Di | 1.10.287.1100, 1 hit |
InterProi | View protein in InterPro IPR015064, Sda IPR036916, Sda_sf |
Pfami | View protein in Pfam PF08970, Sda, 1 hit |
SUPFAMi | SSF100985, SSF100985, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNWVPSMRKL SDELLIESYF KATEMNLNRD FIELIENEIK RRSLGHIISV
SS
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018790 | 1 – 6 | Missing in isoform 2. Curated | 6 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAE01463.1 |
RefSeqi | WP_010886571.1, NC_000964.3 YP_054588.1, NC_000964.3 [Q7WY62-1] |
Genome annotation databases
EnsemblBacteriai | CAE01463; CAE01463; BSU_25690 |
GeneIDi | 2914223 |
KEGGi | bsu:BSU25690 |
PATRICi | fig|224308.43.peg.2679 |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAE01463.1 |
RefSeqi | WP_010886571.1, NC_000964.3 YP_054588.1, NC_000964.3 [Q7WY62-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1PV0 | NMR | - | A | 7-52 | [»] | |
3FYR | X-ray | 1.97 | A/B/C | 7-52 | [»] | |
BMRBi | Q7WY62 | |||||
SASBDBi | Q7WY62 | |||||
SMRi | Q7WY62 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | Q7WY62, 1 interactor |
STRINGi | 224308.BSU25690 |
Proteomic databases
PaxDbi | Q7WY62 |
PRIDEi | Q7WY62 |
Genome annotation databases
EnsemblBacteriai | CAE01463; CAE01463; BSU_25690 |
GeneIDi | 2914223 |
KEGGi | bsu:BSU25690 |
PATRICi | fig|224308.43.peg.2679 |
Phylogenomic databases
eggNOGi | ENOG5033C8I, Bacteria |
OMAi | MKCLPDD |
Enzyme and pathway databases
BioCyci | BSUB:BSU25690-MONOMER |
Miscellaneous databases
EvolutionaryTracei | Q7WY62 |
Family and domain databases
Gene3Di | 1.10.287.1100, 1 hit |
InterProi | View protein in InterPro IPR015064, Sda IPR036916, Sda_sf |
Pfami | View protein in Pfam PF08970, Sda, 1 hit |
SUPFAMi | SSF100985, SSF100985, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SDA_BACSU | |
Accessioni | Q7WY62Primary (citable) accession number: Q7WY62 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2004 |
Last sequence update: | October 1, 2003 | |
Last modified: | June 2, 2021 | |
This is version 107 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references