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UniProtKB - Q7WSH3 (FADD3_COMTE)
Protein
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl:CoA ligase
Gene
fadD3
Organism
Comamonas testosteroni (Pseudomonas testosteroni)
Status
Functioni
Involved in the catabolism of the rings C and D of cholesterol. Catalyzes the ATP-dependent CoA thioesterification of 3aalpha-H-4alpha(3'-propanoate)-7abeta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA.
1 PublicationCatalytic activityi
- 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + ATP + CoA = 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA + AMP + diphosphateBy similarityEC:6.2.1.41By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 413 | ATPBy similarity | 1 | |
Binding sitei | 428 | ATPBy similarity | 1 | |
Binding sitei | 519 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 189 – 197 | ATPBy similarity | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ligase activity Source: UniProtKB-KW
GO - Biological processi
- cholesterol metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Biological process | Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl:CoA ligase1 Publication (EC:6.2.1.41By similarity)Short name: HIP:CoA ligase1 Publication |
Gene namesi | Name:fadD3By similarity Synonyms:ORF181 Publication |
Organismi | Comamonas testosteroni (Pseudomonas testosteroni) |
Taxonomic identifieri | 285 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Comamonas |
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene accumulate 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid (HIP) when incubated with 1,4-androstadiene-3,17-dione (ADD).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000430640 | 1 – 540 | 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl:CoA ligaseAdd BLAST | 540 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
Q7WSH3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKPNQNAMP ESSLTLPGML ADVVSRFAGR AAIVENGKSI SYAQLQQLSR
60 70 80 90 100
LAARALMSLG VQAGDRVALW APNLSEWIVA ACGVHAAGGV LVPLNTRMKG
110 120 130 140 150
AEAADILERS RARVLVCVGD FLNNYYPDLL NGLRPVTLQQ VVVLGDKVLP
160 170 180 190 200
SADMNWAQFM ARADGTTAEA QQQREARIKP DDTADLMFTS GTTGRPKGVM
210 220 230 240 250
CAHRPTILAF KAWSDVVGLT EGSRYLIVNP FFHTFGYKAG WVAALLQGST
260 270 280 290 300
VYPEQIFDAQ AILHRIESDR ISFLPGPPTL FLSMLAHPGL KNFDLSSLKS
310 320 330 340 350
SVTGASTVPP ILIKRMREEL GIMNVTTAYG LTECGGCATL CEPSDNVETV
360 370 380 390 400
ANTCGKALPG TEVRCVDEQG RPVGPGEAGE VLLRGYHIMQ GYFEDEKATE
410 420 430 440 450
ETIDADGWLH TGDVGVLDER GYLRITDRLK DMFIVGGFNC YPAEIERMLS
460 470 480 490 500
NHPEVAQVAV VGVADERMGE VGCACVVTRN GVTLDQEAFI AWCRANMANY
510 520 530 540
KVPRFVLQLD GLPVNASNKV QKRDLLQIVK DRLASVPQPA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB076368 Genomic DNA Translation: BAC81695.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB076368 Genomic DNA Translation: BAC81695.1 |
3D structure databases
SMRi | Q7WSH3 |
ModBasei | Search... |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FADD3_COMTE | |
Accessioni | Q7WSH3Primary (citable) accession number: Q7WSH3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 29, 2014 |
Last sequence update: | October 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 40 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |