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Protein

Thymidylate synthase

Gene

thyA

Organism
Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21dUMPUniRule annotation1
Active sitei192NucleophileUniRule annotation1
Binding sitei2175,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei225dUMPUniRule annotation1
Binding sitei3225,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi172 – 173dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi214 – 217dUMPUniRule annotation4
Nucleotide bindingi255 – 257dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:BB0872
OrganismiBordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) (Alcaligenes bronchisepticus)
Taxonomic identifieri257310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
Proteomesi
  • UP000001027 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409381 – 323Thymidylate synthaseAdd BLAST323

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi257310.BB0872

Structurei

3D structure databases

ProteinModelPortaliQ7WP13
SMRiQ7WP13
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V Bacteria
COG0207 LUCA
HOGENOMiHOG000257900
KOiK00560
OMAiKQYLDLC
OrthoDBiPOG091H02L6

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 2 hits
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q7WP13-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQYLDLVQS ILDQGAWQEN RTGVRTLSLP GAALRFDLQQ GFPAVTTKKL
60 70 80 90 100
AFKSAIGEMV GFLRATRSAA QFRALGCKVW DQNANENEQW LANPYREGPD
110 120 130 140 150
DLGPVYGVQW RHWPAYKLLP RSAGGQVADA LARGYRQVAE VAENGAPHVL
160 170 180 190 200
LYKAVDQLRQ CLDTIHQSPG DRRILFHGWN WAQIEEMALP PCHLLYQFLP
210 220 230 240 250
NAGTREISLC LYIRSNDVGL GTPFNLTEGA ALLHLVGRLT GYKPRWFSYF
260 270 280 290 300
IGDAHVYENH LPMLREQLTR EPYPAPQLVL SDRIPDFAVT GKYEPQWLEQ
310 320
VEPGDFTLSG YQHHAPLTAP MAV
Length:323
Mass (Da):36,406
Last modified:October 1, 2003 - v1
Checksum:i39E014AE19B3C0AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX640439 Genomic DNA Translation: CAE31371.1
RefSeqiWP_003808442.1, NC_002927.3

Genome annotation databases

EnsemblBacteriaiCAE31371; CAE31371; BB0872
KEGGibbr:BB0872

Similar proteinsi

Entry informationi

Entry nameiTYSY_BORBR
AccessioniPrimary (citable) accession number: Q7WP13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2003
Last modified: October 25, 2017
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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