Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Blochmannia floridanus
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Bifunctional protein GlmU (glmU)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II), the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional protein GlmU (glmU)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: LPS lipid A biosynthesis

This protein is involved in the pathway LPS lipid A biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway LPS lipid A biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei25UDP-GlcNAcUniRule annotation1
Binding sitei80UDP-GlcNAcUniRule annotation1
Metal bindingi110MagnesiumUniRule annotation1
Binding sitei145UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei160UDP-GlcNAcUniRule annotation1
Metal bindingi235MagnesiumUniRule annotation1
Binding sitei235UDP-GlcNAcUniRule annotation1
Binding sitei341UDP-GlcNAcUniRule annotation1
Binding sitei359UDP-GlcNAcUniRule annotation1
Active sitei371Proton acceptorUniRule annotation1
Binding sitei374UDP-GlcNAcUniRule annotation1
Binding sitei385UDP-GlcNAcUniRule annotation1
Binding sitei388Acetyl-CoA; via amide nitrogenUniRule annotation1
Binding sitei431Acetyl-CoA; via amide nitrogenUniRule annotation1
Binding sitei448Acetyl-CoAUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciCBLO203907:G1GJ5-10-MONOMER
UniPathwayiUPA00113; UER00532
UPA00113; UER00533
UPA00973

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmUUniRule annotation
Including the following 2 domains:
UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
Gene namesi
Name:glmUUniRule annotation
Ordered Locus Names:Bfl010
OrganismiBlochmannia floridanus
Taxonomic identifieri203907 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia
Proteomesi
  • UP000002192 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002337381 – 465Bifunctional protein GlmUAdd BLAST465

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi203907.Bfl010

Structurei

3D structure databases

ProteinModelPortaliQ7VQV4
SMRiQ7VQV4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 237PyrophosphorylaseUniRule annotationAdd BLAST237
Regioni11 – 14UDP-GlcNAc bindingUniRule annotation4
Regioni85 – 86UDP-GlcNAc bindingUniRule annotation2
Regioni108 – 110UDP-GlcNAc bindingUniRule annotation3
Regioni238 – 258LinkerUniRule annotationAdd BLAST21
Regioni259 – 465N-acetyltransferaseUniRule annotationAdd BLAST207
Regioni394 – 395Acetyl-CoA bindingUniRule annotation2

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation
In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105CAJ Bacteria
COG1207 LUCA
HOGENOMiHOG000283476
KOiK04042
OMAiIEPQTHL
OrthoDBiPOG091H02I2

Family and domain databases

CDDicd03353 LbH_GlmU_C, 1 hit
Gene3Di3.90.550.10, 1 hit
HAMAPiMF_01631 GlmU, 1 hit
InterProiView protein in InterPro
IPR005882 Bifunctional_GlmU
IPR038009 GlmU_C_LbH
IPR001451 Hexapep
IPR018357 Hexapep_transf_CS
IPR025877 MobA-like_NTP_Trfase
IPR029044 Nucleotide-diphossugar_trans
IPR011004 Trimer_LpxA-like_sf
PfamiView protein in Pfam
PF00132 Hexapep, 3 hits
PF14602 Hexapep_2, 1 hit
PF12804 NTP_transf_3, 1 hit
SUPFAMiSSF51161 SSF51161, 1 hit
SSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR01173 glmU, 1 hit
PROSITEiView protein in PROSITE
PS00101 HEXAPEP_TRANSFERASES, 1 hit

Sequencei

Sequence statusi: Complete.

Q7VQV4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRKKYSVII LAAGQGKRML SSIPKVMHKI AGRSMLQHLI DSVSKINIQS
60 70 80 90 100
IYIVYNESLR EFIPTIYSNK CTISIYWVLQ ESVRGTGYAV QQVLSMIHDD
110 120 130 140 150
NEDILILYGD VPLISDKTLN NLCLMKSKCD IGLLTAYVKN PQGYGRIIRN
160 170 180 190 200
QYGDVVSIIE DIDIVNQNDR IIKEIHSGIF ISVSGYLKSW LNYLIENNLW
210 220 230 240 250
CNELYLTAII DIAYKNNFVI NAIHPVDVCE VIGINNKSDL VKAERVYQIK
260 270 280 290 300
EAQKLLELGV MISDLNRFDL RGKLVCGEDV CIDVNVIIEG CVSLGNRVKI
310 320 330 340 350
GASCILKDVE IGDDVIIYPF SFIENSKIHC KSKIGPFARL RPGTCLQEQV
360 370 380 390 400
HIGNFVELKN IKLGNNSKVG HLSYLGDADV GNYVNIGAGT IICNYNGKTK
410 420 430 440 450
NYTCIEDNVF VGADSQLVAP ITIGKNAVIG AGTTVTQNVD KDDVIISRVR
460
QFSIVNDKVD KNKKN
Length:465
Mass (Da):51,831
Last modified:October 1, 2003 - v1
Checksum:iF651C7E6DEF99D8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248583 Genomic DNA Translation: CAD83538.1
RefSeqiWP_011126308.1, NC_005061.1

Genome annotation databases

EnsemblBacteriaiCAD83538; CAD83538; Bfl010
KEGGibfl:Bfl010

Similar proteinsi

Entry informationi

Entry nameiGLMU_BLOFL
AccessioniPrimary (citable) accession number: Q7VQV4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: October 1, 2003
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health