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Protein

Lipoyl synthase 1

Gene

lipA1

Organism
Prochlorococcus marinus subsp. pastoris (strain CCMP1986 / NIES-2087 / MED4)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase 1 (lipA1), Lipoyl synthase 2 (lipA2)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi41Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi46Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi52Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi67Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi71Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi74Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPMAR59919:TX50_RS08120-MONOMER
UniPathwayi
UPA00538;UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 1UniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-syn 1UniRule annotation
Lipoate synthase 1UniRule annotation
Lipoic acid synthase 1UniRule annotation
Sulfur insertion protein lipA1
Gene namesi
Name:lipA1UniRule annotation
Synonyms:lipA
Ordered Locus Names:PMM1514
OrganismiProchlorococcus marinus subsp. pastoris (strain CCMP1986 / NIES-2087 / MED4)
Taxonomic identifieri59919 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesProchloraceaeProchlorococcus
Proteomesi
  • UP000001026 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001023401 – 297Lipoyl synthase 1Add BLAST297

Interactioni

Protein-protein interaction databases

STRINGi59919.PMM1514

Structurei

3D structure databases

ProteinModelPortaliQ7UZY1
SMRiQ7UZY1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0G Bacteria
COG0320 LUCA
HOGENOMiHOG000235997
KOiK03644
OMAiPYCDIDF
OrthoDBiPOG091H069D

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR003698 Lipoyl_synth
IPR007197 rSAM
PANTHERiPTHR10949 PTHR10949, 1 hit
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDF00271 lipoyl_synthase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

Q7UZY1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTNISKNAVT KPDWLRVKAP QVERIGNTAN LLSDLKLNTV CQEASCPNIG
60 70 80 90 100
ECFASGTATF LIMGPGCTRA CPYCDIDFDR SKRDLDPTEP DRLAEAVFRL
110 120 130 140 150
KLKHVVITSV NRDDLDDGGA SQFYKCVSEV RKKSPETTIE LLIPDLCGNW
160 170 180 190 200
SALEKILDSR PNVLNHNIET VSALYRKVRP QGNYQRTLEL LKRTREYFPS
210 220 230 240 250
VYTKSGFMLG LGEKDDEVLT LLMDLRKNDV DIVTIGQYLS PGPKHLQVQR
260 270 280 290
FVTPSKFNYF KLFGENELGF MQVVSSPLTR SSYHAEEIQK LMKKFPR
Length:297
Mass (Da):33,662
Last modified:October 1, 2003 - v1
Checksum:iE17EE4F68A67FC89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX548174 Genomic DNA Translation: CAE19973.1
RefSeqiWP_011133142.1, NC_005072.1

Genome annotation databases

EnsemblBacteriaiCAE19973; CAE19973; PMM1514
KEGGipmm:PMM1514

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX548174 Genomic DNA Translation: CAE19973.1
RefSeqiWP_011133142.1, NC_005072.1

3D structure databases

ProteinModelPortaliQ7UZY1
SMRiQ7UZY1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi59919.PMM1514

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE19973; CAE19973; PMM1514
KEGGipmm:PMM1514

Phylogenomic databases

eggNOGiENOG4105C0G Bacteria
COG0320 LUCA
HOGENOMiHOG000235997
KOiK03644
OMAiPYCDIDF
OrthoDBiPOG091H069D

Enzyme and pathway databases

UniPathwayi
UPA00538;UER00593

BioCyciPMAR59919:TX50_RS08120-MONOMER

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR003698 Lipoyl_synth
IPR007197 rSAM
PANTHERiPTHR10949 PTHR10949, 1 hit
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDF00271 lipoyl_synthase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLIPA1_PROMP
AccessioniPrimary (citable) accession number: Q7UZY1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: October 1, 2003
Last modified: October 10, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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