UniProtKB - Q7U2U1 (GMHBA_MYCBO)
Protein
D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase
Gene
gmhB
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Functioni
Converts the D-glycero-alpha-D-manno-heptose 1,7-bisphosphate intermediate into D-glycero-alpha-D-manno-heptose 1-phosphate by removing the phosphate group at the C-7 position.By similarity
Catalytic activityi
- D-glycero-α-D-manno-heptose 1,7-bisphosphate + H2O = D-glycero-α-D-manno-heptose 1-phosphate + phosphateEC:3.1.3.83
Cofactori
Protein has several cofactor binding sites:: GDP-D-glycero-alpha-D-manno-heptose biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes GDP-D-glycero-alpha-D-manno-heptose from D-glycero-alpha-D-manno-heptose 7-phosphate.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- no protein annotated in this organism
- D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase (gmhB)
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GDP-D-glycero-alpha-D-manno-heptose from D-glycero-alpha-D-manno-heptose 7-phosphate, the pathway GDP-D-glycero-alpha-D-manno-heptose biosynthesis and in Nucleotide-sugar biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 15 | NucleophileBy similarity | 1 | |
Metal bindingi | 15 | MagnesiumBy similarity | 1 | |
Active sitei | 17 | Proton donorBy similarity | 1 | |
Metal bindingi | 17 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Sitei | 59 | Stabilizes the phosphoryl groupBy similarity | 1 | |
Metal bindingi | 98 | ZincBy similarity | 1 | |
Metal bindingi | 100 | Zinc; via pros nitrogenBy similarity | 1 | |
Metal bindingi | 106 | ZincBy similarity | 1 | |
Metal bindingi | 108 | ZincBy similarity | 1 | |
Sitei | 109 | Contributes to substrate recognitionBy similarity | 1 | |
Sitei | 110 | Stabilizes the phosphoryl groupBy similarity | 1 | |
Metal bindingi | 136 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- D,D-heptose 1,7-bisphosphate phosphatase activity Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- carbohydrate metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Biological process | Carbohydrate metabolism |
Ligand | Magnesium, Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | MBOV233413:G1GT4-122-MONOMER |
UniPathwayi | UPA00543;UER00607 |
Names & Taxonomyi
Protein namesi | Recommended name: D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase (EC:3.1.3.83)Alternative name(s): D,D-heptose 1,7-bisphosphate phosphatase Short name: HBP phosphatase |
Gene namesi | Name:gmhB Ordered Locus Names:BQ2027_MB0118 |
Organismi | Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) |
Taxonomic identifieri | 233413 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209396 | 1 – 190 | D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphataseAdd BLAST | 190 |
Interactioni
Subunit structurei
Monomer.
By similarityFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 15 – 17 | Substrate bindingBy similarity | 3 | |
Regioni | 23 – 28 | Substrate bindingBy similarity | 6 | |
Regioni | 59 – 62 | Substrate bindingBy similarity | 4 | |
Regioni | 109 – 110 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the GmhB family.Curated
Phylogenomic databases
OMAi | GNRAGCR |
Family and domain databases
Gene3Di | 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR036412, HAD-like_sf IPR006549, HAD-SF_hydro_IIIA IPR023214, HAD_sf IPR004446, Heptose_bisP_phosphatase IPR006543, Histidinol-phos |
PANTHERi | PTHR42891, PTHR42891, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR01662, HAD-SF-IIIA, 1 hit TIGR01656, Histidinol-ppas, 1 hit |
i Sequence
Sequence statusi: Complete.
Q7U2U1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVAERAGHQW CLFLDRDGVI NRQVVGDYVR NWRQFEWLPG AARALKKLRA
60 70 80 90 100
WAPYIVVVTN QQGVGAGLMS AVDVMVIHRH LQMQLASDGV LIDGFQVCPH
110 120 130 140 150
HRSQRCGCRK PRPGLVLDWL RRHPDSEPLL SIVVGDSLSD LELAHNVAAA
160 170 180 190
AGACASVQIG GASSGGVADA SFDSLWEFAV AVGHARGERG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | LT708304 Genomic DNA Translation: SIT98525.1 |
RefSeqi | NP_853785.1, NC_002945.3 WP_010950343.1, NC_002945.4 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | LT708304 Genomic DNA Translation: SIT98525.1 |
RefSeqi | NP_853785.1, NC_002945.3 WP_010950343.1, NC_002945.4 |
3D structure databases
SMRi | Q7U2U1 |
ModBasei | Search... |
Phylogenomic databases
OMAi | GNRAGCR |
Enzyme and pathway databases
UniPathwayi | UPA00543;UER00607 |
BioCyci | MBOV233413:G1GT4-122-MONOMER |
Family and domain databases
Gene3Di | 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR036412, HAD-like_sf IPR006549, HAD-SF_hydro_IIIA IPR023214, HAD_sf IPR004446, Heptose_bisP_phosphatase IPR006543, Histidinol-phos |
PANTHERi | PTHR42891, PTHR42891, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR01662, HAD-SF-IIIA, 1 hit TIGR01656, Histidinol-ppas, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GMHBA_MYCBO | |
Accessioni | Q7U2U1Primary (citable) accession number: Q7U2U1 Secondary accession number(s): A0A1R3XVI5, X2BE37 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2004 |
Last sequence update: | October 1, 2003 | |
Last modified: | April 7, 2021 | |
This is version 93 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families