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Protein

Activating transcription factor 7-interacting protein 1

Gene

Atf7ip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. Required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9' (H3K9me3). The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3). May have ATPase activity.2 Publications

GO - Molecular functioni

  • ATPase activity Source: MGI
  • transcription corepressor activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionActivator, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-3214841 PKMTs methylate histone lysines

Names & Taxonomyi

Protein namesi
Recommended name:
Activating transcription factor 7-interacting protein 1
Alternative name(s):
ATFa-associated modulator
Short name:
mAM
MBD1-containing chromatin-associated factor 1
Gene namesi
Name:Atf7ip
Synonyms:Mcaf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1858965 Atf7ip

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002817811 – 1306Activating transcription factor 7-interacting protein 1Add BLAST1306

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Cross-linki33Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei57PhosphoserineCombined sources1
Modified residuei112PhosphoserineCombined sources1
Modified residuei124PhosphothreonineBy similarity1
Modified residuei511PhosphoserineBy similarity1
Modified residuei514PhosphoserineCombined sources1
Modified residuei516PhosphoserineCombined sources1
Modified residuei533PhosphoserineBy similarity1
Cross-linki592Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei593PhosphoserineCombined sources1
Modified residuei700PhosphoserineCombined sources1
Modified residuei707PhosphoserineBy similarity1
Modified residuei933PhosphoserineBy similarity1
Cross-linki944Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki974Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ7TT18
PaxDbiQ7TT18
PeptideAtlasiQ7TT18
PRIDEiQ7TT18

PTM databases

iPTMnetiQ7TT18
PhosphoSitePlusiQ7TT18

Expressioni

Tissue specificityi

Ubiquitously expressed at all stages studied.1 Publication

Gene expression databases

BgeeiENSMUSG00000030213 Expressed in 285 organ(s), highest expression level in mesenteric lymph node
ExpressionAtlasiQ7TT18 baseline and differential
GenevisibleiQ7TT18 MM

Interactioni

Subunit structurei

Interacts with MBD1; the interaction is enhanced when MBD1 is sumoylated. Probably forms a complex with SETDB1 and MBD1. Interacts with SUMO ubiquitin-like proteins (SUMO1, SUNO2 and SUMO3), with a preference for SUMO2 and SUMO3. Interacts with SP1 and ZHX1 (By similarity). Interacts with the general transcription machinery, including ERCC2, ERCC3, GTF2E1, GTF2E2 and POLR2A (By similarity). Interacts with SETDB1 and ATF7.By similarity1 Publication

Protein-protein interaction databases

BioGridi207616, 13 interactors
IntActiQ7TT18, 5 interactors
MINTiQ7TT18
STRINGi10090.ENSMUSP00000032335

Structurei

3D structure databases

ProteinModelPortaliQ7TT18
SMRiQ7TT18
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1054 – 1143Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST90
Domaini1196 – 1302Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST107

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni596 – 851Interaction with SETDB1By similarityAdd BLAST256
Regioni1001 – 1011Interaction with SUMOBy similarityAdd BLAST11
Regioni1190 – 1306Interaction with MBD1By similarityAdd BLAST117

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili666 – 696Sequence analysisAdd BLAST31

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi587 – 605Nuclear localization signal1 PublicationAdd BLAST19

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi189 – 259Ser-richAdd BLAST71
Compositional biasi385 – 614Glu-richAdd BLAST230
Compositional biasi929 – 1036Ser-richAdd BLAST108

Sequence similaritiesi

Belongs to the MCAF family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IJ5P Eukaryota
ENOG410XUWA LUCA
GeneTreeiENSGT00530000063707
HOVERGENiHBG087180
InParanoidiQ7TT18
OMAiQVGIPFK
OrthoDBiEOG091G058O
PhylomeDBiQ7TT18
TreeFamiTF329427

Family and domain databases

Gene3Di2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR026085 ATF7-int
IPR031870 ATF7IP_BD
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
PANTHERiPTHR23210 PTHR23210, 1 hit
PfamiView protein in Pfam
PF16788 ATF7IP_BD, 1 hit
PF16794 fn3_4, 1 hit
SUPFAMiSSF49265 SSF49265, 1 hit
PROSITEiView protein in PROSITE
PS50853 FN3, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q7TT18-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDSVEEPQKK VFKARKTMRA SDRQQLDAVH RVKGELLRAD GKLLNGSHEN
60 70 80 90 100
GDLDPTSPLE NTDCIQDREE VNGIDGICFQ SEESTTEWKE TPCMPNVAVK
110 120 130 140 150
NKQEDLNSEA LSPSITCDLS SRVTTEPGSG SPASDNPGCG TPVSDNPASD
160 170 180 190 200
NPASDNPASD NPDSGDLAAG ELATTVQATG DSACEEPPSS DPSSSDPTSS
210 220 230 240 250
EPSSSEPTCS EPISGDPVSE EAASHDLVSG DSTCSEPVSG EPVSHEAASS
260 270 280 290 300
EPATSEPASD EPVARVVAAC ELAPGESALD DCAPSGDSQS DEPPSSEDSL
310 320 330 340 350
PRSVCSGLAS GELTPGELSV EPATDTVKPS SSAVCEAGPD PDKTEPSSNN
360 370 380 390 400
SDDCPGKSED DEHLDQIQSK DSCDEGNKVN SNVVEKEEPL ETHSAIICSD
410 420 430 440 450
LPPENTTKIA EDPIAEPALE EEAISSSMEV DQSEKDEHKS PAEPVAAVSE
460 470 480 490 500
DPAEEDKEDT VVDNTDSMET DEIIPILEKL APTEDELSCF SKASLLPVET
510 520 530 540 550
SQDLEDKMEG SFGSPSKQES SENLPKEAFL VLSDEEDLSC GKDESEAVAQ
560 570 580 590 600
SKMSTPEGEK SEKDGKAEEE ERVPAEEQPP VRNEFSRRKR SKSEDMDSVE
610 620 630 640 650
SKRRRYMDEE YEAEFQVKIT AKGDINQKLQ KVIQWLLQEK LCALQCAVFD
660 670 680 690 700
KTLAELKTRV EKIECNKRHK AVLTELQAKI ARLTKRFGAA KDDLKKRQES
710 720 730 740 750
PPNPPISPGK PANDTNSNNN MTYRNAGTVR QLLESKRNVS EGPPPSFQTP
760 770 780 790 800
VNTVSSASHA TSTAVVSSQP KLQTSATSGS LPAAPLLPAP STATVVATTQ
810 820 830 840 850
VPSGTPQPTI SLQPLPVILH VPVAVTSQPQ LLQSHPGTLV TNQPSGNVEF
860 870 880 890 900
ISVQSQPTVS GLTKNPVSLP PLPNPTKPNI PSVPSPSSIQ RNSSTTAAPL
910 920 930 940 950
GTTLAVQAVP TAHSIVQATR TSLPTVGPSG LYSSSSSRGP IQMKIPISTF
960 970 980 990 1000
SPPSSAEQNS SATPRIVTEN QTNKTVDSSI NKKAADSTSQ SGKASSSDSS
1010 1020 1030 1040 1050
GVIDLTMDDE ESGTTQDPKK ISPPSSSTVS TSQPMSRPLQ PILPAPPLQP
1060 1070 1080 1090 1100
SGVPTSGPSQ ATIHVLPTAP TTVNVTHRPV TQVTTRLPVP RAPANHQVVY
1110 1120 1130 1140 1150
TTLPAPTTQA PLRGTVMQAP AVRQVNPQNS VTVRVPQTTT YVVNNGLTLG
1160 1170 1180 1190 1200
SAGPQLTVHH RPPQVHNEPP RPLHPAPLPE APQPQRLPPE AASTSLPQKP
1210 1220 1230 1240 1250
HLKLARVQSQ NGIVLSWSVL EVDRSCATVD SYHLYAYHEE PSATVPSQWK
1260 1270 1280 1290 1300
KIGEVKALPL PMACTLTQFV SGSKYYFAVR AKDIYGRFGP FCDPQSTDVI

SSSQNS
Length:1,306
Mass (Da):138,593
Last modified:October 1, 2003 - v1
Checksum:iD09FE3ACC93FA207
GO
Isoform 2 (identifier: Q7TT18-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-959: Missing.
     960-989: SSATPRIVTENQTNKTVDSSINKKAADSTS → MRPFSPLPLLLPHISLVASAPLQNQTSFIF

Note: No experimental confirmation available.
Show »
Length:347
Mass (Da):37,258
Checksum:i8F620344A6744ABE
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WQB3A0A087WQB3_MOUSE
Activating transcription factor 7-i...
Atf7ip
157Annotation score:
A0A087WR75A0A087WR75_MOUSE
Activating transcription factor 7-i...
Atf7ip
969Annotation score:
A0A0N4SVP5A0A0N4SVP5_MOUSE
Activating transcription factor 7-i...
Atf7ip
345Annotation score:
A0A0N4SVA8A0A0N4SVA8_MOUSE
Activating transcription factor 7-i...
Atf7ip
73Annotation score:
A0A087WR39A0A087WR39_MOUSE
Activating transcription factor 7-i...
Atf7ip
559Annotation score:
A0A087WNQ2A0A087WNQ2_MOUSE
Activating transcription factor 7-i...
Atf7ip
107Annotation score:
A0A087WPT6A0A087WPT6_MOUSE
Activating transcription factor 7-i...
Atf7ip
46Annotation score:

Sequence cautioni

The sequence BAE39609 differs from that shown. Reason: Frameshift at position 1203.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti121S → Y in BAE22064 (PubMed:16141072).Curated1
Sequence conflicti128G → A in CAB77024 (PubMed:10777215).Curated1
Sequence conflicti128G → A in AAH22714 (PubMed:15489334).Curated1
Sequence conflicti128G → A in AAH48827 (PubMed:15489334).Curated1
Sequence conflicti235S → G in CAB77024 (PubMed:10777215).Curated1
Sequence conflicti235S → G in AAH22714 (PubMed:15489334).Curated1
Sequence conflicti235S → G in AAH48827 (PubMed:15489334).Curated1
Sequence conflicti287D → E in AAH22714 (PubMed:15489334).Curated1
Sequence conflicti287D → E in AAH48827 (PubMed:15489334).Curated1
Sequence conflicti539S → L in CAB77024 (PubMed:10777215).Curated1
Sequence conflicti539S → L in AAH22714 (PubMed:15489334).Curated1
Sequence conflicti539S → L in AAH48827 (PubMed:15489334).Curated1
Sequence conflicti545S → P in BAE39609 (PubMed:16141072).Curated1
Sequence conflicti547A → V in AAH22714 (PubMed:15489334).Curated1
Sequence conflicti547A → V in AAH48827 (PubMed:15489334).Curated1
Sequence conflicti551S → T in CAB77024 (PubMed:10777215).Curated1
Sequence conflicti551S → T in AAH22714 (PubMed:15489334).Curated1
Sequence conflicti551S → T in AAH48827 (PubMed:15489334).Curated1
Sequence conflicti564 – 565DG → GS in CAB77024 (PubMed:10777215).Curated2
Sequence conflicti600 – 602ESK → QST in CAB77024 (PubMed:10777215).Curated3
Sequence conflicti622K → G in CAB77024 (PubMed:10777215).Curated1
Sequence conflicti631K → E in CAB77024 (PubMed:10777215).Curated1
Sequence conflicti634Q → R in CAB77024 (PubMed:10777215).Curated1
Sequence conflicti964P → T in BAE39609 (PubMed:16141072).Curated1
Sequence conflicti968T → A in CAB77024 (PubMed:10777215).Curated1
Sequence conflicti1201H → Q in BAE39609 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0240401 – 959Missing in isoform 2. 1 PublicationAdd BLAST959
Alternative sequenceiVSP_024041960 – 989SSATP…ADSTS → MRPFSPLPLLLPHISLVASA PLQNQTSFIF in isoform 2. 1 PublicationAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132702 mRNA Translation: CAB77024.1
BC022714 mRNA Translation: AAH22714.1 Different termination.
AK082697 mRNA Translation: BAC38574.1
AK134252 mRNA Translation: BAE22064.1
AK137199 mRNA Translation: BAE23264.1
AK157479 mRNA Translation: BAE34096.1
AK167542 mRNA Translation: BAE39609.1 Frameshift.
BC048827 mRNA Translation: AAH48827.1 Different termination.
BC052460 mRNA Translation: AAH52460.1
BC060246 mRNA Translation: AAH60246.1
CCDSiCCDS20651.1 [Q7TT18-1]
RefSeqiNP_062299.2, NM_019426.2 [Q7TT18-1]
XP_006506452.2, XM_006506389.3 [Q7TT18-1]
XP_017177170.1, XM_017321681.1 [Q7TT18-1]
UniGeneiMm.173271
Mm.412064
Mm.491167

Genome annotation databases

EnsembliENSMUST00000032335; ENSMUSP00000032335; ENSMUSG00000030213 [Q7TT18-1]
GeneIDi54343
KEGGimmu:54343
UCSCiuc009elv.1 mouse [Q7TT18-1]
uc009elx.1 mouse [Q7TT18-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132702 mRNA Translation: CAB77024.1
BC022714 mRNA Translation: AAH22714.1 Different termination.
AK082697 mRNA Translation: BAC38574.1
AK134252 mRNA Translation: BAE22064.1
AK137199 mRNA Translation: BAE23264.1
AK157479 mRNA Translation: BAE34096.1
AK167542 mRNA Translation: BAE39609.1 Frameshift.
BC048827 mRNA Translation: AAH48827.1 Different termination.
BC052460 mRNA Translation: AAH52460.1
BC060246 mRNA Translation: AAH60246.1
CCDSiCCDS20651.1 [Q7TT18-1]
RefSeqiNP_062299.2, NM_019426.2 [Q7TT18-1]
XP_006506452.2, XM_006506389.3 [Q7TT18-1]
XP_017177170.1, XM_017321681.1 [Q7TT18-1]
UniGeneiMm.173271
Mm.412064
Mm.491167

3D structure databases

ProteinModelPortaliQ7TT18
SMRiQ7TT18
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207616, 13 interactors
IntActiQ7TT18, 5 interactors
MINTiQ7TT18
STRINGi10090.ENSMUSP00000032335

PTM databases

iPTMnetiQ7TT18
PhosphoSitePlusiQ7TT18

Proteomic databases

EPDiQ7TT18
PaxDbiQ7TT18
PeptideAtlasiQ7TT18
PRIDEiQ7TT18

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032335; ENSMUSP00000032335; ENSMUSG00000030213 [Q7TT18-1]
GeneIDi54343
KEGGimmu:54343
UCSCiuc009elv.1 mouse [Q7TT18-1]
uc009elx.1 mouse [Q7TT18-2]

Organism-specific databases

CTDi55729
MGIiMGI:1858965 Atf7ip

Phylogenomic databases

eggNOGiENOG410IJ5P Eukaryota
ENOG410XUWA LUCA
GeneTreeiENSGT00530000063707
HOVERGENiHBG087180
InParanoidiQ7TT18
OMAiQVGIPFK
OrthoDBiEOG091G058O
PhylomeDBiQ7TT18
TreeFamiTF329427

Enzyme and pathway databases

ReactomeiR-MMU-3214841 PKMTs methylate histone lysines

Miscellaneous databases

PROiPR:Q7TT18
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030213 Expressed in 285 organ(s), highest expression level in mesenteric lymph node
ExpressionAtlasiQ7TT18 baseline and differential
GenevisibleiQ7TT18 MM

Family and domain databases

Gene3Di2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR026085 ATF7-int
IPR031870 ATF7IP_BD
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
PANTHERiPTHR23210 PTHR23210, 1 hit
PfamiView protein in Pfam
PF16788 ATF7IP_BD, 1 hit
PF16794 fn3_4, 1 hit
SUPFAMiSSF49265 SSF49265, 1 hit
PROSITEiView protein in PROSITE
PS50853 FN3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMCAF1_MOUSE
AccessioniPrimary (citable) accession number: Q7TT18
Secondary accession number(s): Q3TJ86
, Q3TZW1, Q3UVK5, Q3UYZ7, Q80ZK7, Q8C4A1, Q9JK31
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 1, 2003
Last modified: November 7, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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