UniProtKB - Q7TSJ2 (MAP6_MOUSE)
Protein
Microtubule-associated protein 6
Gene
Map6
Organism
Mus musculus (Mouse)
Status
Functioni
Involved in microtubule stabilization in many cell types, including neuronal cells (PubMed:9600916). Specifically has microtubule cold stabilizing activity (PubMed:9600916). Involved in dendrite morphogenesis and maintenance by regulating lysosomal trafficking via its interaction with TMEM106B (By similarity). Regulates KIF5A-mediated axonal cargo transport (By similarity). Regulates axonal growth during neuron polarization (PubMed:28521134).By similarity2 Publications
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
- microtubule binding Source: MGI
GO - Biological processi
- axonal transport of mitochondrion Source: MGI
- cytoskeleton-dependent intracellular transport Source: GO_Central
- dendrite morphogenesis Source: UniProtKB
- lysosome localization Source: UniProtKB
- microtubule cytoskeleton organization Source: InterPro
- positive regulation of axonogenesis Source: MGI
- regulation of microtubule cytoskeleton organization Source: MGI
Keywordsi
Molecular function | Calmodulin-binding |
Biological process | Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Microtubule-associated protein 6Short name: MAP-6 Alternative name(s): Stable tubule-only polypeptide Short name: STOP |
Gene namesi | Name:Map6 Synonyms:Mtap6 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1201690, Map6 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus 1 Publication
Cytoskeleton
- cytoskeleton 1 Publication
Other locations
- axon By similarity
- dendrite By similarity
- secretory vesicle membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
Note: Isoform 1 and isoform 2 associate with axonal microtubules in neurons (PubMed:16837464). Isoform 3 associates with microtubules in fibroblasts (PubMed:9600916). Localizes predominantly in the proximal part of the axon (By similarity). Preferentially is concentrated on a portion of the microtubule polymer in which tubulin is modified by detyrosination and acetylation and is also resistant to depolymerization induced by both nocodazole and cold (By similarity). In unpolarized neurons, localizes to the Golgi and to secretory vesicles accumulating transiently at the tips of a subset of neurites (By similarity). Following neuronal polarization and during axon outgrowth, accumulates in the axonal growth cone and subsequently localizes throughout the axon. Partially localizes to dendrites in mature neurons (By similarity).By similarity2 Publications
Cytoskeleton
- microtubule Source: MGI
Golgi apparatus
- cis-Golgi network Source: GO_Central
- Golgi apparatus Source: MGI
Other locations
- axon Source: MGI
- axon cytoplasm Source: GOC
- dendrite Source: MGI
- Golgi-associated vesicle Source: GO_Central
- neuron projection Source: MGI
- perinuclear region of cytoplasm Source: MGI
- secretory vesicle Source: MGI
- transport vesicle membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Golgi apparatus, Membrane, MicrotubulePathology & Biotechi
Disruption phenotypei
Mice are devoid of cold-stable microtubules and show no detectable defects in brain anatomy but show synaptic defects, with depleted synaptic vesicle pools and impaired synaptic plasticity, associated with severe behavioral disorders, including a disorganized activity with disruption of normal behavioral sequences and episodes of hyperlocomotion or apparent prostration, anxiety, severe social withdrawal and complete nurturing defects (PubMed:12231625). The behavioral defects are alleviated by long-term treatment with neuroleptics (PubMed:12231625). RNAi-mediated knockdown in brain at the 14.5 dpc stage disrupts proper neuron migration resulting in their accumulation in the ventricular and subventricular zones (PubMed:28521134).2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000344045 | 1 – 906 | Microtubule-associated protein 6Add BLAST | 906 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 5 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 10 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 11 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 98 | PhosphoserineCombined sources | 1 | |
Modified residuei | 141 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 185 | PhosphoserineBy similarity | 1 | |
Modified residuei | 207 | PhosphoserineCombined sources | 1 | |
Modified residuei | 632 | PhosphoserineCombined sources | 1 | |
Modified residuei | 687 | PhosphoserineCombined sources | 1 | |
Modified residuei | 905 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Palmitoylated. Probably depalmitoylated by ABHD17A, ABHD17B and ABHD17C. During neuronal polarization, palmitoylation and depalmitoylation cycles regulate MAP6 shuttling between secretory vesicles and microtubules, and its polarized distribution in the axon.By similarity
Keywords - PTMi
Lipoprotein, Palmitate, PhosphoproteinProteomic databases
PaxDbi | Q7TSJ2 |
PeptideAtlasi | Q7TSJ2 |
PRIDEi | Q7TSJ2 |
PTM databases
iPTMneti | Q7TSJ2 |
PhosphoSitePlusi | Q7TSJ2 |
SwissPalmi | Q7TSJ2 |
Expressioni
Tissue specificityi
Isoform 1 is specifically expressed in adult brain. Isoform 2 is predominantly expressed in embryonic brain; expression persists at low levels in the adult brain. Isoform 3 is expressed at high levels in lung and at lower levels in testis, heart, muscle and kidney (at protein level). Oligodendrocytes express a major isoform of 89 kDa (O-STOP). Astrocytes also express an isoform of 60 kDa (A-STOP).2 Publications
Developmental stagei
Isoform 2 is expressed in embryonic brain.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000055407, Expressed in forebrain and 262 other tissues |
Genevisiblei | Q7TSJ2, MM |
Interactioni
Subunit structurei
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
- microtubule binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201587, 16 interactors |
IntActi | Q7TSJ2, 8 interactors |
MINTi | Q7TSJ2 |
STRINGi | 10090.ENSMUSP00000064787 |
Miscellaneous databases
RNActi | Q7TSJ2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 222 – 267 | Mc-1By similarityAdd BLAST | 46 | |
Repeati | 268 – 313 | Mc-2By similarityAdd BLAST | 46 | |
Repeati | 314 – 359 | Mc-3By similarityAdd BLAST | 46 | |
Repeati | 360 – 405 | Mc-4By similarityAdd BLAST | 46 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 15 | Calmodulin-bindingBy similarityAdd BLAST | 15 | |
Regioni | 116 – 139 | Mn 1By similarityAdd BLAST | 24 | |
Regioni | 124 – 138 | Calmodulin-bindingBy similarityAdd BLAST | 15 | |
Regioni | 151 – 174 | Mn 2By similarityAdd BLAST | 24 | |
Regioni | 160 – 174 | Calmodulin-bindingBy similarityAdd BLAST | 15 | |
Regioni | 187 – 201 | Calmodulin-bindingBy similarityAdd BLAST | 15 | |
Regioni | 222 – 405 | 4 X approximate tandem repeat McBy similarityAdd BLAST | 184 | |
Regioni | 235 – 249 | Calmodulin-bindingBy similarityAdd BLAST | 15 | |
Regioni | 280 – 294 | Calmodulin-bindingBy similarityAdd BLAST | 15 | |
Regioni | 325 – 339 | Calmodulin-bindingBy similarityAdd BLAST | 15 | |
Regioni | 375 – 389 | Calmodulin-bindingBy similarityAdd BLAST | 15 | |
Regioni | 427 – 450 | Mn 3By similarityAdd BLAST | 24 | |
Regioni | 435 – 449 | Calmodulin-bindingBy similarityAdd BLAST | 15 | |
Regioni | 486 – 500 | Calmodulin-bindingBy similarityAdd BLAST | 15 | |
Regioni | 513 – 527 | Calmodulin-bindingBy similarityAdd BLAST | 15 |
Sequence similaritiesi
Belongs to the STOP family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG502QS1F, Eukaryota |
GeneTreei | ENSGT00530000063947 |
HOGENOMi | CLU_018049_1_0_1 |
InParanoidi | Q7TSJ2 |
OMAi | KKPGPAW |
OrthoDBi | 577082at2759 |
PhylomeDBi | Q7TSJ2 |
TreeFami | TF338320 |
Family and domain databases
InterProi | View protein in InterPro IPR007882, MAP6 |
PANTHERi | PTHR14759, PTHR14759, 6 hits |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative promoter usage and alternative splicing. AlignAdd to basketNote: Comment: Additional isoforms seem to exist.
This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q7TSJ2-1) [UniParc]FASTAAdd to basket
Also known as: N-STOP, Neuronal STOP
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAWPCITRAC CIARFWNQLD KADIAVPLVF TKYSEATEHP GAPPQPPAPL
60 70 80 90 100
QPALAPPSRA VAIETQPAQG ESDAVARATG PAPGPSVDRE TVAAPGRSGL
110 120 130 140 150
GLGAASASTS GSGPADSVMR QDYRAWKVQR PEPSCRPRSE YQPSDAPFER
160 170 180 190 200
ETQYQKDFRA WPLPRRGDHP WIPKPVQIPA TSQPSQPVLG VPKRRPQSQE
210 220 230 240 250
RGPMQLSADA RDPEGAGGAG VLAAGKASGV DQRDTRRKAG PAWMVTRNEG
260 270 280 290 300
HEEKPLPPAQ SQTQEGGPAA GKASGADQRD TRRKAGPAWM VTRSEGHEEK
310 320 330 340 350
PLPPAQSQTQ EGGPAAGKAS GADQRDTRRK AGPAWMVTRT EGHEETPLPP
360 370 380 390 400
AQSQTQEGGP AAGKASGADE RDTRRKAGPA WMVRRSEGHE QTPAAHAQGT
410 420 430 440 450
GPEGGKGRAV ADALNRQIRE EVASTVSSSY RNEFRAWTDI KPVKPIKAKP
460 470 480 490 500
QYKPPDDKMV HETSYSAQFK GEANKPSAAD NKAMDRRRIR SLYSEPFKEC
510 520 530 540 550
PKVEKPSVQS SKPKKTSTSH KPPRKAKDKQ VVSGQAAKKK TTEGPSATKP
560 570 580 590 600
DDKEQSKEMN NKLAEAKESR VKPTSDASKN RGPVTKEPHK DQGSVAPGLP
610 620 630 640 650
KGQEPLKDQG PVVPGLPKDQ VPVVPGSLKG QSPTAPGPTK DQGAVLLGPV
660 670 680 690 700
KDLGPVAPAP IKVQDHIASE LLKNKDSVPL APAKAQSPLL PEPLKNQSPV
710 720 730 740 750
VPASTKDQSF PTPAPRKDPG PVIPEPEKDR APTVPERRKD QHVSIMASLK
760 770 780 790 800
NEAPMVPESV KNQGLAGPEL VKDTGTDTTA PRYLKGHDSV FVAPVKNQGP
810 820 830 840 850
VIPEPVKSQD PIIPALAKDQ GPMLPEPPKN QSPVVLGPIK NQDPIIPVPL
860 870 880 890 900
KGQDPLVPAP TKDQGPTAPD PLKTQGPKGT QLPTVSPSPP VMIPTVPHTE
YIEGSP
Note: Produced by alternative promoter usage.
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3Z6W1 | D3Z6W1_MOUSE | Microtubule-associated protein 6 | Map6 Mtap6 | 509 | Annotation score: | ||
A0A140LIT9 | A0A140LIT9_MOUSE | Microtubule-associated protein 6 | Map6 | 703 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 81 | P → L in CAA75049 (PubMed:9501006).Curated | 1 | |
Sequence conflicti | 87 | V → G in CAA75930 (PubMed:9501006).Curated | 1 | |
Sequence conflicti | 344 | E → K in CAA75930 (PubMed:9501006).Curated | 1 | |
Sequence conflicti | 344 | E → K in CAA75989 (PubMed:9600916).Curated | 1 | |
Sequence conflicti | 405 | G → S in CAA75049 (PubMed:9501006).Curated | 1 | |
Sequence conflicti | 755 – 757 | MVP → VVH in CAA75930 (PubMed:9501006).Curated | 3 | |
Sequence conflicti | 755 | M → V in CAA75049 (PubMed:9501006).Curated | 1 | |
Sequence conflicti | 825 | P → S in CAA75930 (PubMed:9501006).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_034724 | 1 – 203 | Missing in isoform 3. 1 PublicationAdd BLAST | 203 | |
Alternative sequenceiVSP_034725 | 503 – 509 | VEKPSVQ → EPGQTHQ in isoform 3. 1 Publication | 7 | |
Alternative sequenceiVSP_034726 | 510 – 906 | Missing in isoform 3. 1 PublicationAdd BLAST | 397 | |
Alternative sequenceiVSP_034727 | 569 – 906 | Missing in isoform 2. 1 PublicationAdd BLAST | 338 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y14754, Y14755, Y14756 Genomic DNA Translation: CAA75049.1 Y16008 Genomic DNA Translation: CAA75930.1 Y16032 mRNA Translation: CAA75989.1 BC053039 mRNA Translation: AAH53039.1 |
CCDSi | CCDS21479.1 [Q7TSJ2-1] CCDS40029.1 [Q7TSJ2-3] CCDS85345.1 [Q7TSJ2-2] |
PIRi | JC5963 |
RefSeqi | NP_001036820.2, NM_001043355.2 [Q7TSJ2-3] NP_001041632.1, NM_001048167.1 [Q7TSJ2-2] NP_034967.2, NM_010837.3 [Q7TSJ2-1] |
Genome annotation databases
Ensembli | ENSMUST00000068973; ENSMUSP00000064787; ENSMUSG00000055407 [Q7TSJ2-1] ENSMUST00000107100; ENSMUSP00000102717; ENSMUSG00000055407 [Q7TSJ2-3] ENSMUST00000127492; ENSMUSP00000146340; ENSMUSG00000055407 [Q7TSJ2-2] ENSMUST00000207883; ENSMUSP00000146585; ENSMUSG00000055407 [Q7TSJ2-1] ENSMUST00000208605; ENSMUSP00000146897; ENSMUSG00000055407 [Q7TSJ2-3] |
GeneIDi | 17760 |
KEGGi | mmu:17760 |
UCSCi | uc009ilg.1, mouse [Q7TSJ2-1] uc009ili.1, mouse [Q7TSJ2-3] |
Keywords - Coding sequence diversityi
Alternative promoter usage, Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y14754, Y14755, Y14756 Genomic DNA Translation: CAA75049.1 Y16008 Genomic DNA Translation: CAA75930.1 Y16032 mRNA Translation: CAA75989.1 BC053039 mRNA Translation: AAH53039.1 |
CCDSi | CCDS21479.1 [Q7TSJ2-1] CCDS40029.1 [Q7TSJ2-3] CCDS85345.1 [Q7TSJ2-2] |
PIRi | JC5963 |
RefSeqi | NP_001036820.2, NM_001043355.2 [Q7TSJ2-3] NP_001041632.1, NM_001048167.1 [Q7TSJ2-2] NP_034967.2, NM_010837.3 [Q7TSJ2-1] |
3D structure databases
SMRi | Q7TSJ2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201587, 16 interactors |
IntActi | Q7TSJ2, 8 interactors |
MINTi | Q7TSJ2 |
STRINGi | 10090.ENSMUSP00000064787 |
PTM databases
iPTMneti | Q7TSJ2 |
PhosphoSitePlusi | Q7TSJ2 |
SwissPalmi | Q7TSJ2 |
Proteomic databases
PaxDbi | Q7TSJ2 |
PeptideAtlasi | Q7TSJ2 |
PRIDEi | Q7TSJ2 |
Protocols and materials databases
Antibodypediai | 17382, 60 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000068973; ENSMUSP00000064787; ENSMUSG00000055407 [Q7TSJ2-1] ENSMUST00000107100; ENSMUSP00000102717; ENSMUSG00000055407 [Q7TSJ2-3] ENSMUST00000127492; ENSMUSP00000146340; ENSMUSG00000055407 [Q7TSJ2-2] ENSMUST00000207883; ENSMUSP00000146585; ENSMUSG00000055407 [Q7TSJ2-1] ENSMUST00000208605; ENSMUSP00000146897; ENSMUSG00000055407 [Q7TSJ2-3] |
GeneIDi | 17760 |
KEGGi | mmu:17760 |
UCSCi | uc009ilg.1, mouse [Q7TSJ2-1] uc009ili.1, mouse [Q7TSJ2-3] |
Organism-specific databases
CTDi | 4135 |
MGIi | MGI:1201690, Map6 |
Phylogenomic databases
eggNOGi | ENOG502QS1F, Eukaryota |
GeneTreei | ENSGT00530000063947 |
HOGENOMi | CLU_018049_1_0_1 |
InParanoidi | Q7TSJ2 |
OMAi | KKPGPAW |
OrthoDBi | 577082at2759 |
PhylomeDBi | Q7TSJ2 |
TreeFami | TF338320 |
Miscellaneous databases
BioGRID-ORCSi | 17760, 2 hits in 17 CRISPR screens |
ChiTaRSi | Map6, mouse |
PROi | PR:Q7TSJ2 |
RNActi | Q7TSJ2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000055407, Expressed in forebrain and 262 other tissues |
Genevisiblei | Q7TSJ2, MM |
Family and domain databases
InterProi | View protein in InterPro IPR007882, MAP6 |
PANTHERi | PTHR14759, PTHR14759, 6 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MAP6_MOUSE | |
Accessioni | Q7TSJ2Primary (citable) accession number: Q7TSJ2 Secondary accession number(s): O55129 Q78DV5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 22, 2008 |
Last sequence update: | July 22, 2008 | |
Last modified: | February 10, 2021 | |
This is version 121 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families