UniProtKB - Q7TSH2 (KPBB_MOUSE)
Protein
Phosphorylase b kinase regulatory subunit beta
Gene
Phkb
Organism
Mus musculus (Mouse)
Status
Functioni
Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation (By similarity).By similarity
Activity regulationi
By phosphorylation of various serine residues.By similarity
: glycogen metabolism Pathwayi
This protein is involved in the pathway glycogen metabolism, which is part of Glycan biosynthesis.View all proteins of this organism that are known to be involved in the pathway glycogen metabolism and in Glycan biosynthesis.
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
GO - Biological processi
- glycogen metabolic process Source: UniProtKB-UniPathway
- protein phosphorylation Source: MGI
Keywordsi
Molecular function | Calmodulin-binding |
Biological process | Carbohydrate metabolism, Glycogen metabolism |
Enzyme and pathway databases
Reactomei | R-MMU-70221, Glycogen breakdown (glycogenolysis) |
UniPathwayi | UPA00163 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphorylase b kinase regulatory subunit betaShort name: Phosphorylase kinase subunit beta |
Gene namesi | Name:Phkb |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97578, Phkb |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- phosphorylase kinase complex Source: MGI
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000057737 | 1 – 1085 | Phosphorylase b kinase regulatory subunit betaAdd BLAST | 1085 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 10 | PhosphoserineBy similarity | 1 | |
Modified residuei | 19 | PhosphoserineCombined sources | 1 | |
Modified residuei | 693 | PhosphoserineBy similarity | 1 | |
Lipidationi | 1082 | S-farnesyl cysteineBy similarity | 1 |
Post-translational modificationi
Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.By similarity
Keywords - PTMi
Lipoprotein, Phosphoprotein, PrenylationProteomic databases
EPDi | Q7TSH2 |
jPOSTi | Q7TSH2 |
MaxQBi | Q7TSH2 |
PaxDbi | Q7TSH2 |
PeptideAtlasi | Q7TSH2 |
PRIDEi | Q7TSH2 |
PTM databases
iPTMneti | Q7TSH2 |
PhosphoSitePlusi | Q7TSH2 |
SwissPalmi | Q7TSH2 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000036879, Expressed in quadriceps femoris and 284 other tissues |
ExpressionAtlasi | Q7TSH2, baseline and differential |
Genevisiblei | Q7TSH2, MM |
Interactioni
Subunit structurei
Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin (By similarity).
By similarityGO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 221792, 4 interactors |
IntActi | Q7TSH2, 1 interactor |
STRINGi | 10090.ENSMUSP00000050788 |
Miscellaneous databases
RNActi | Q7TSH2, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 760 – 787 | Calmodulin-bindingSequence analysisAdd BLAST | 28 | |
Regioni | 912 – 943 | Calmodulin-bindingSequence analysisAdd BLAST | 32 |
Sequence similaritiesi
Belongs to the phosphorylase b kinase regulatory chain family.Curated
Phylogenomic databases
eggNOGi | KOG3635, Eukaryota |
GeneTreei | ENSGT00950000183118 |
HOGENOMi | CLU_004177_0_1_1 |
InParanoidi | Q7TSH2 |
OMAi | CQLWGIL |
OrthoDBi | 55049at2759 |
PhylomeDBi | Q7TSH2 |
TreeFami | TF313970 |
Family and domain databases
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR011613, GH15-like IPR008734, PHK_A/B_su |
PANTHERi | PTHR10749, PTHR10749, 1 hit |
Pfami | View protein in Pfam PF00723, Glyco_hydro_15, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
Q7TSH2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MANSPDAAFS SPALLRSGSV YEPLKSINLP RPDNETLWDK LDHYYRIVKS
60 70 80 90 100
TMLMYQSPTT GLFPTKTCGG EEKSKVHESL YCAAGAWALA LAYRRIDDDK
110 120 130 140 150
GRTHELEHSA IKCMRGILYC YMRQADKVQQ FKQDPRPTTC LHSVFSVHTG
160 170 180 190 200
DELLSYEEYG HLQINAVSLF LLYLVEMISS GLQIIYNTDE VSFIQNLVFC
210 220 230 240 250
VERVYRVPDF GVWERGSKYN NGSTELHSSS VGLAKAALEA INGFNLFGNQ
260 270 280 290 300
GCSWSVIFVD LDAHNRNRQT LCSLLPRESR SHNTDAALLP CISYPAFALD
310 320 330 340 350
DEALFSQTLD KVIRKLKGKY GFKRFLRDGY RTPLEDPNRR YYKPAEIKLF
360 370 380 390 400
DGIECEFPIF FLYMMIDGVF RGNLEQVKEY QDLLTPLLHQ TTEGYPVVPK
410 420 430 440 450
YYYVPADFVE CEKRNPGSQK RFPSNCGRDG KLFLWGQALY IIAKLLADEL
460 470 480 490 500
ISPKDIDPVQ RFVPLQNQRN VSMRYSNQGP LENDLVVHVA LVAESQRLQV
510 520 530 540 550
FLNTYGIQTQ TPQQVEPIQI WPQQELVKAY FHLGINEKLG LSGRPDRPIG
560 570 580 590 600
CLGTSKIYRI LGKTVVCYPI IFDLSDFYMS QDVLLLIDDI KNALQFIKQY
610 620 630 640 650
WKMHGRPLFL VLIREDNIRG SRFNPILDML AAFKKGIIGG VKVHVDRLQT
660 670 680 690 700
LISGAVVEQL DFLRISDTEK LPEFKSFEEL EFPKHSKVKR QSSTADAPEA
710 720 730 740 750
QHEPGITITE WKNKSTHEIL QKLNDCGCLA GQTILLGILL KREGPNFITM
760 770 780 790 800
EGTVSDHIER VYRRAGSKKL WSVVRRAASL LNKVVDSLAP SITNVLVQGK
810 820 830 840 850
QVTLGAFGHE EEVISNPLSP RVIKNIIYYK CNTHDEREAV IQQELVIHIG
860 870 880 890 900
WIISNSPELF SGMLKIRIGW IIHAMEYELQ VRGGDKPAVD LYQLSPSEVK
910 920 930 940 950
QLLLDILQPQ QSGRCWLNRR QIDGSLNRTP PEFYDRVWQI LERTPNGIVV
960 970 980 990 1000
AGKHLPQQPT LSDMTMYEMN FSLLVEDMLG NIDQPKYRQI IVELLMVVSI
1010 1020 1030 1040 1050
VLERNPELEF QDKVDLDRLV KEAFHEFQKD ESRLKEIEKQ DDMTSFYNTP
1060 1070 1080
PLGKRGTCSY LTKVVMNSLL EGEVKPSNED SCLVS
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE0CX43 | E0CX43_MOUSE | Phosphorylase b kinase regulatory s... | Phkb | 206 | Annotation score: | ||
E0CXM1 | E0CXM1_MOUSE | Phosphorylase b kinase regulatory s... | Phkb | 163 | Annotation score: | ||
E0CZ55 | E0CZ55_MOUSE | Phosphorylase b kinase regulatory s... | Phkb | 169 | Annotation score: | ||
F7DF70 | F7DF70_MOUSE | Phosphorylase b kinase regulatory s... | Phkb | 314 | Annotation score: | ||
E0CY30 | E0CY30_MOUSE | Phosphorylase b kinase regulatory s... | Phkb | 40 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC053105 mRNA Translation: AAH53105.1 |
CCDSi | CCDS22501.1 |
RefSeqi | NP_955517.1, NM_199446.1 XP_006530595.1, XM_006530532.3 XP_006530596.1, XM_006530533.3 XP_006530597.1, XM_006530534.3 |
Genome annotation databases
Ensembli | ENSMUST00000053771; ENSMUSP00000050788; ENSMUSG00000036879 |
GeneIDi | 102093 |
KEGGi | mmu:102093 |
UCSCi | uc009mqj.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC053105 mRNA Translation: AAH53105.1 |
CCDSi | CCDS22501.1 |
RefSeqi | NP_955517.1, NM_199446.1 XP_006530595.1, XM_006530532.3 XP_006530596.1, XM_006530533.3 XP_006530597.1, XM_006530534.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 221792, 4 interactors |
IntActi | Q7TSH2, 1 interactor |
STRINGi | 10090.ENSMUSP00000050788 |
PTM databases
iPTMneti | Q7TSH2 |
PhosphoSitePlusi | Q7TSH2 |
SwissPalmi | Q7TSH2 |
Proteomic databases
EPDi | Q7TSH2 |
jPOSTi | Q7TSH2 |
MaxQBi | Q7TSH2 |
PaxDbi | Q7TSH2 |
PeptideAtlasi | Q7TSH2 |
PRIDEi | Q7TSH2 |
Protocols and materials databases
Antibodypediai | 28116, 165 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000053771; ENSMUSP00000050788; ENSMUSG00000036879 |
GeneIDi | 102093 |
KEGGi | mmu:102093 |
UCSCi | uc009mqj.1, mouse |
Organism-specific databases
CTDi | 5257 |
MGIi | MGI:97578, Phkb |
Phylogenomic databases
eggNOGi | KOG3635, Eukaryota |
GeneTreei | ENSGT00950000183118 |
HOGENOMi | CLU_004177_0_1_1 |
InParanoidi | Q7TSH2 |
OMAi | CQLWGIL |
OrthoDBi | 55049at2759 |
PhylomeDBi | Q7TSH2 |
TreeFami | TF313970 |
Enzyme and pathway databases
UniPathwayi | UPA00163 |
Reactomei | R-MMU-70221, Glycogen breakdown (glycogenolysis) |
Miscellaneous databases
BioGRID-ORCSi | 102093, 0 hits in 17 CRISPR screens |
ChiTaRSi | Phkb, mouse |
PROi | PR:Q7TSH2 |
RNActi | Q7TSH2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036879, Expressed in quadriceps femoris and 284 other tissues |
ExpressionAtlasi | Q7TSH2, baseline and differential |
Genevisiblei | Q7TSH2, MM |
Family and domain databases
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR011613, GH15-like IPR008734, PHK_A/B_su |
PANTHERi | PTHR10749, PTHR10749, 1 hit |
Pfami | View protein in Pfam PF00723, Glyco_hydro_15, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KPBB_MOUSE | |
Accessioni | Q7TSH2Primary (citable) accession number: Q7TSH2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 4, 2005 |
Last sequence update: | October 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families