Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

HCLS1-associated protein X-1

Gene

Hax1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recruits the Arp2/3 complex to the cell cortex and regulates reorganization of the cortical actin cytoskeleton via its interaction with KCNC3 and the Arp2/3 complex. Slows down the rate of inactivation of KCNC3 channels. Promotes GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. Promotes cell survival. May regulate intracellular calcium pools.By similarity2 Publications

GO - Molecular functioni

  • protein domain specific binding Source: RGD

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
HCLS1-associated protein X-1
Alternative name(s):
HS1-associating protein X-1
Short name:
HAX-1
HS1-binding protein 1
Short name:
HSP1BP-1
Gene namesi
Name:Hax1
Synonyms:Hs1bp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi727960 Hax1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Mitochondrion, Nucleus, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003138012 – 278HCLS1-associated protein X-1Add BLAST277

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei188PhosphoserineBy similarity1
Modified residuei191PhosphoserineBy similarity1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei126 – 127Cleavage; by caspase-3By similarity2

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ7TSE9
PRIDEiQ7TSE9

Expressioni

Tissue specificityi

Present in striated muscles (at protein level).1 Publication

Developmental stagei

Abundant in hindlimb and cardiac muscles throughout embryogenesis (at protein level).1 Publication

Interactioni

Subunit structurei

Interacts with ABCB1, ABCB4 and ABCB11 (PubMed:15159385). Directly associates with HCLS1/HS1, through binding to its N-terminal region (By similarity). Interacts with CTTN (PubMed:15159385). Interacts with PKD2. Interacts with GNA13. Interacts with CASP9. Interacts with ITGB6. Interacts with PLN and ATP2A2; these interactions are inhibited by calcium. Interacts with GRB7. Interacts (via C-terminus) with XIAP/BIRC4 (via BIR 2 domain and BIR 3 domain) and this interaction blocks ubiquitination of XIAP/BIRC4. Interacts with TPC2. Interacts with KCNC3. Interacts with XPO1 (By similarity).By similarity1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ7TSE9, 4 interactors
STRINGi10116.ENSRNOP00000067159

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 43Required for localization in mitochondriaAdd BLAST42
Regioni113 – 278Involved in HCLS1 bindingBy similarityAdd BLAST166
Regioni174 – 205Involved in CASP9 bindingBy similarityAdd BLAST32
Regioni175 – 246Involved in GNA13 bindingBy similarityAdd BLAST72
Regioni182 – 278Required for localization in sarcoplasmic reticulumAdd BLAST97
Regioni183 – 278Involved in PKD2 bindingBy similarityAdd BLAST96
Regioni202 – 244Involved in ATP2A2 bindingBy similarityAdd BLAST43
Regioni202 – 224Involved in PLN bindingBy similarityAdd BLAST23
Regioni269 – 278Required for ITGB6 bindingBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi30 – 42Asp/Glu-rich (highly acidic)Add BLAST13

Sequence similaritiesi

Belongs to the HAX1 family.Curated

Phylogenomic databases

HOVERGENiHBG002991
InParanoidiQ7TSE9
KOiK16220
PhylomeDBiQ7TSE9

Family and domain databases

InterProiView protein in InterPro
IPR017248 HAX-1
PANTHERiPTHR14938 PTHR14938, 1 hit
PIRSFiPIRSF037634 HS1-associating_X-1, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 5 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q7TSE9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSVFDLFRGF FGFPGPRSHR DPFFGGMTRD DDDDEDDEEE EDSGAWGRES
60 70 80 90 100
YAFDGFHPTE EFGFSFSPRG GMRFHGNFGF DDLVRDFNSI FSEMGAWTLP
110 120 130 140 150
SHSPELPGPE SETPGVRLRE GQTLRDSMLK YPDSHQPRIF EGVLESHAKP
160 170 180 190 200
ESSKPAPDWG SQGPFHRLDD TWPVSPHSRA REDKDLDSQV SQEGLGPLLQ
210 220 230 240 250
PQPKSYFKSI SVTKITKPDG TVEEHRTVVD SEGRRETTVT HQEAHDSSRS
260 270
DPDPPRSSAL DDPFSILDLL LGRWFRSR
Length:278
Mass (Da):31,448
Last modified:October 1, 2003 - v1
Checksum:i0B49E32FF89CD975
GO
Isoform 2 (identifier: Q7TSE9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     44-104: Missing.

Show »
Length:217
Mass (Da):24,572
Checksum:i822F48152513778E
GO
Isoform 3 (identifier: Q7TSE9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MSVFDLFRGFFGFPGPRS → MQQGPERRKQWGSGKEDREQVIG

Show »
Length:283
Mass (Da):32,084
Checksum:iE7BB60A4895ECB21
GO
Isoform 5 (identifier: Q7TSE9-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Show »
Length:252
Mass (Da):28,488
Checksum:iD3163FE42C27D946
GO
Isoform 6 (identifier: Q7TSE9-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.
     44-104: Missing.

Show »
Length:191
Mass (Da):21,611
Checksum:iF0C0BA00B2450DA2
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
M0R639M0R639_RAT
HCLS1-associated protein X-1
Hax1
184Annotation score:
M0RC50M0RC50_RAT
HCLS1-associated protein X-1
Hax1
181Annotation score:
A0A096MKE1A0A096MKE1_RAT
HCLS1-associated protein X-1
Hax1
158Annotation score:
A0A096MK08A0A096MK08_RAT
HCLS1-associated protein X-1
Hax1
97Annotation score:
A0A096MJ14A0A096MJ14_RAT
HCLS1-associated protein X-1
Hax1
123Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0301381 – 26Missing in isoform 5 and isoform 6. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_0301371 – 18MSVFD…PGPRS → MQQGPERRKQWGSGKEDREQ VIG in isoform 3. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_03013944 – 104Missing in isoform 2 and isoform 6. 1 PublicationAdd BLAST61

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY291062 mRNA Translation: AAP44974.1
AY291063 mRNA Translation: AAP44975.1
AY291064 mRNA Translation: AAP44976.1
AY919342 mRNA Translation: AAX18866.1
AY919343 mRNA Translation: AAX18867.1
DQ286293 Genomic DNA Translation: ABB91376.1
RefSeqiNP_853658.1, NM_181627.2 [Q7TSE9-1]
UniGeneiRn.185269

Genome annotation databases

GeneIDi291202
KEGGirno:291202

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY291062 mRNA Translation: AAP44974.1
AY291063 mRNA Translation: AAP44975.1
AY291064 mRNA Translation: AAP44976.1
AY919342 mRNA Translation: AAX18866.1
AY919343 mRNA Translation: AAX18867.1
DQ286293 Genomic DNA Translation: ABB91376.1
RefSeqiNP_853658.1, NM_181627.2 [Q7TSE9-1]
UniGeneiRn.185269

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7TSE9, 4 interactors
STRINGi10116.ENSRNOP00000067159

Proteomic databases

PaxDbiQ7TSE9
PRIDEiQ7TSE9

Protocols and materials databases

DNASUi291202
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi291202
KEGGirno:291202

Organism-specific databases

CTDi10456
RGDi727960 Hax1

Phylogenomic databases

HOVERGENiHBG002991
InParanoidiQ7TSE9
KOiK16220
PhylomeDBiQ7TSE9

Miscellaneous databases

PROiPR:Q7TSE9

Family and domain databases

InterProiView protein in InterPro
IPR017248 HAX-1
PANTHERiPTHR14938 PTHR14938, 1 hit
PIRSFiPIRSF037634 HS1-associating_X-1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHAX1_RAT
AccessioniPrimary (citable) accession number: Q7TSE9
Secondary accession number(s): Q5D1N3
, Q5D1N4, Q7TSE7, Q7TSE8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2003
Last modified: November 7, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again