UniProtKB - Q7TS75 (AMER1_MOUSE)
Protein
APC membrane recruitment protein 1
Gene
Amer1
Organism
Mus musculus (Mouse)
Status
Functioni
Regulator of the canonical Wnt signaling pathway. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex. Acts both as a positive and negative regulator of the Wnt signaling pathway, depending on the context: acts as a positive regulator by promoting LRP6 phosphorylation. Also acts as a negative regulator by acting as a scaffold protein for the beta-catenin destruction complex and promoting stabilization of Axin at the cell membrane. Promotes CTNNB1 ubiquitination and degradation. Involved in kidney development (By similarity).By similarity
GO - Molecular functioni
- beta-catenin binding Source: UniProtKB
- beta-catenin destruction complex binding Source: MGI
- phosphatidylinositol-4,5-bisphosphate binding Source: UniProtKB
GO - Biological processi
- adipose tissue development Source: MGI
- bone development Source: MGI
- kidney development Source: MGI
- mesenchymal cell differentiation involved in kidney development Source: MGI
- negative regulation of canonical Wnt signaling pathway Source: UniProtKB
- positive regulation of canonical Wnt signaling pathway Source: UniProtKB
- positive regulation of cellular protein catabolic process Source: MGI
- positive regulation of protein ubiquitination Source: MGI
- regulation of canonical Wnt signaling pathway Source: UniProtKB
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Biological process | Wnt signaling pathway |
Ligand | Lipid-binding |
Enzyme and pathway databases
Reactomei | R-MMU-195253, Degradation of beta-catenin by the destruction complex R-MMU-196299, Beta-catenin phosphorylation cascade R-MMU-4641262, Disassembly of the destruction complex and recruitment of AXIN to the membrane |
Names & Taxonomyi
Protein namesi | Recommended name: APC membrane recruitment protein 1Short name: Amer1 Alternative name(s): Protein FAM123B |
Gene namesi | Name:Amer1 Synonyms:Fam123b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919595, Amer1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Nucleus
- Nucleus By similarity
Other locations
- Cytoplasm By similarity
Note: Shuttles between nucleus and cytoplasm. Detected in nuclear paraspeckles that are found close to splicing speckles. Translocates to the cell membrane following binding to PtdIns(4,5)P2 (By similarity).By similarity
Nucleus
- nuclear body Source: MGI
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB-SubCell
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000281888 | 1 – 1132 | APC membrane recruitment protein 1Add BLAST | 1132 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | Q7TS75 |
MaxQBi | Q7TS75 |
PaxDbi | Q7TS75 |
PeptideAtlasi | Q7TS75 |
PRIDEi | Q7TS75 |
PTM databases
iPTMneti | Q7TS75 |
PhosphoSitePlusi | Q7TS75 |
Expressioni
Tissue specificityi
Expressed in kidney.1 Publication
Developmental stagei
In embryos, it is highly expressed in the neonatal brain and kidney and then declines substantially in the mature organs. Also expressed in lung and spleen. Expressed in the condensing metanephric mesenchyme and in early epithelial structures that are precursors to glomeruli.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000050332, Expressed in embryo and 288 other tissues |
Genevisiblei | Q7TS75, MM |
Interactioni
Subunit structurei
Interacts with CTNNB1, AXIN1, LRP6, KEAP1, APC and BTRC.
Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes containing BTRC and/or FBXW11. Identified in the beta-catenin destruction complex containing CTNNB1, APC, AXIN1 and AXIN2.
Interacts with WT1 (By similarity).
By similarityGO - Molecular functioni
- beta-catenin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 215325, 5 interactors |
STRINGi | 10090.ENSMUSP00000109502 |
Miscellaneous databases
RNActi | Q7TS75, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 375 – 381 | Poly-Asp | 7 | |
Compositional biasi | 382 – 427 | Glu-richAdd BLAST | 46 | |
Compositional biasi | 756 – 766 | Poly-GluAdd BLAST | 11 | |
Compositional biasi | 934 – 942 | Poly-Glu | 9 |
Sequence similaritiesi
Belongs to the Amer family.Curated
Phylogenomic databases
eggNOGi | ENOG502QT5W, Eukaryota |
GeneTreei | ENSGT00530000063529 |
HOGENOMi | CLU_009351_2_0_1 |
InParanoidi | Q7TS75 |
OMAi | FELGYYQ |
OrthoDBi | 139922at2759 |
PhylomeDBi | Q7TS75 |
TreeFami | TF333006 |
Family and domain databases
InterProi | View protein in InterPro IPR019003, AMER |
PANTHERi | PTHR22237, PTHR22237, 1 hit |
Pfami | View protein in Pfam PF09422, WTX, 2 hits |
i Sequence
Sequence statusi: Complete.
Q7TS75-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MESQQDEAVQ TKGASTSSDA QDQGAEKGAK NKTTEATEGP TSEPPLSGPG
60 70 80 90 100
RLKKTAMKLF GGKKGICTLP SFFGGGRSKG SGKVSSKKSL NKSKTHDGLS
110 120 130 140 150
EASQGPEDVV IEETDLSTPL SKSSAQFPSS QSANGALEIG SKHKTSGTEA
160 170 180 190 200
IEKAGVEKVP SVHKPKKSLK SFFSSIRRHR KGKTSGADQS VPGAKELEGA
210 220 230 240 250
RTRSHEHVSS ISLPSSEEIF RDTRKENAKP QDAPGPKMSP AQVHFSPTTE
260 270 280 290 300
KAACKNPEKL TRTCASEFMQ PKPVLEGGSL EEPHTSETEG KVVAGEVNPP
310 320 330 340 350
NGPVGDQLSL LFGDVTSLKS FDSLTGCGDI IAEQDMDSMT DSMASGGQRA
360 370 380 390 400
NRDGTKRSSC LVTYQGGGEE MALPDDDDND DEEEEEEEEE EEEEEEEEEE
410 420 430 440 450
EEEEEEEEEL LEDEEEVKDG EENDDLEYLW ASAQIYPRFN MNLGYHTAIS
460 470 480 490 500
PSHQGYMLLD PVQSYPNLGL GELLTPQSDQ QESAPNSDEG YYDSTTPGFE
510 520 530 540 550
DDSGEALGLA HRDCLPRDSY SGDALYEFYE PDDSLEHSPP GDDCLYDLRG
560 570 580 590 600
RNSEMLDPFL NLEPFSSRPP GAMETEEERL VTIQKQLLYW ELRREQREAQ
610 620 630 640 650
EACAREAHAR EAYARDTHTR ESYGRNVRAR ETQALEAHSQ EGRVQETKVR
660 670 680 690 700
QEKPALEYQM RPLGPSVMGL VAGTSGGSQT SHRGTTSAFP ATSSSEPDWR
710 720 730 740 750
DFRPLEKRFE GTCSKKDQST CLMQLFQSDA MFEPDMQEAN FGGSPRKAYP
760 770 780 790 800
SYSPPEEPEE EEEEKEGNAT VSFSQALVEF TSNGNLFTSM SYSSDSDSSF
810 820 830 840 850
TQNLPELPPM VTFDIADVER DGEGKCEENP EFNNDEDLTA SLEAFELGYY
860 870 880 890 900
HKHAFNSYHS RFYQGLPWGV SSLPRYLGLP GVHPRPPPAA MALNRRSRSL
910 920 930 940 950
DNAESLELEL SSSHLAQGYM ESDELQAHQE DSDEEGEEEE GEWGRDSPLS
960 970 980 990 1000
LYTEPPGVYD WPPWAHCPLP VGPGLAWMSP NQLYEPFNQS SYVQATCCVP
1010 1020 1030 1040 1050
PVAMPVSVPG RTPGDSVSQL ARPSHLPLPM GPCYNLQSQA SQSGRAKPRD
1060 1070 1080 1090 1100
VLLPVDEPSC SSISGANSQS QAKPVGITHG IPQLPRVRPE PFQLQPNHYR
1110 1120 1130
ASNLDLSKER GEQGASLSTS YSSTAMNGNL AK
Sequence cautioni
The sequence AAH53442 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAC25373 differs from that shown. Reason: Frameshift.Curated
The sequence BAC33914 differs from that shown. Reason: Erroneous termination. Truncated C-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL671765 Genomic DNA No translation available. AK049774 mRNA Translation: BAC33914.1 Sequence problems. AK012651 mRNA Translation: BAC25373.1 Frameshift. BC053442 mRNA Translation: AAH53442.1 Sequence problems. |
CCDSi | CCDS41067.1 |
RefSeqi | NP_780388.2, NM_175179.4 |
Genome annotation databases
Ensembli | ENSMUST00000084535; ENSMUSP00000109502; ENSMUSG00000050332 |
GeneIDi | 72345 |
KEGGi | mmu:72345 |
UCSCi | uc009ttx.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL671765 Genomic DNA No translation available. AK049774 mRNA Translation: BAC33914.1 Sequence problems. AK012651 mRNA Translation: BAC25373.1 Frameshift. BC053442 mRNA Translation: AAH53442.1 Sequence problems. |
CCDSi | CCDS41067.1 |
RefSeqi | NP_780388.2, NM_175179.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 215325, 5 interactors |
STRINGi | 10090.ENSMUSP00000109502 |
PTM databases
iPTMneti | Q7TS75 |
PhosphoSitePlusi | Q7TS75 |
Proteomic databases
jPOSTi | Q7TS75 |
MaxQBi | Q7TS75 |
PaxDbi | Q7TS75 |
PeptideAtlasi | Q7TS75 |
PRIDEi | Q7TS75 |
Protocols and materials databases
Antibodypediai | 43710, 100 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000084535; ENSMUSP00000109502; ENSMUSG00000050332 |
GeneIDi | 72345 |
KEGGi | mmu:72345 |
UCSCi | uc009ttx.2, mouse |
Organism-specific databases
CTDi | 139285 |
MGIi | MGI:1919595, Amer1 |
Phylogenomic databases
eggNOGi | ENOG502QT5W, Eukaryota |
GeneTreei | ENSGT00530000063529 |
HOGENOMi | CLU_009351_2_0_1 |
InParanoidi | Q7TS75 |
OMAi | FELGYYQ |
OrthoDBi | 139922at2759 |
PhylomeDBi | Q7TS75 |
TreeFami | TF333006 |
Enzyme and pathway databases
Reactomei | R-MMU-195253, Degradation of beta-catenin by the destruction complex R-MMU-196299, Beta-catenin phosphorylation cascade R-MMU-4641262, Disassembly of the destruction complex and recruitment of AXIN to the membrane |
Miscellaneous databases
BioGRID-ORCSi | 72345, 1 hit in 17 CRISPR screens |
ChiTaRSi | Amer1, mouse |
PROi | PR:Q7TS75 |
RNActi | Q7TS75, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000050332, Expressed in embryo and 288 other tissues |
Genevisiblei | Q7TS75, MM |
Family and domain databases
InterProi | View protein in InterPro IPR019003, AMER |
PANTHERi | PTHR22237, PTHR22237, 1 hit |
Pfami | View protein in Pfam PF09422, WTX, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AMER1_MOUSE | |
Accessioni | Q7TS75Primary (citable) accession number: Q7TS75 Secondary accession number(s): B1AUM2, Q8BT92, Q8C7P7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | March 21, 2012 | |
Last modified: | December 2, 2020 | |
This is version 102 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families