UniProtKB - Q7TPV2 (DZIP3_MOUSE)
E3 ubiquitin-protein ligase DZIP3
Dzip3
Functioni
E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA.
By similarityCatalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.By similarity EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1144 – 1184 | RING-type; atypicalPROSITE-ProRule annotationAdd BLAST | 41 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- phosphatase binding Source: UniProtKB
- polyubiquitin modification-dependent protein binding Source: MGI
- RNA binding Source: MGI
- ubiquitin protein ligase activity Source: InterPro
- ubiquitin-protein transferase activity Source: MGI
GO - Biological processi
- protein polyubiquitination Source: MGI
Keywordsi
Molecular function | RNA-binding, Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase DZIP3 (EC:2.3.2.27By similarity)Alternative name(s): DAZ-interacting protein 3 homolog RING-type E3 ubiquitin transferase DZIP3Curated |
Gene namesi | Name:Dzip3 Synonyms:Kiaa0675 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1917433, Dzip3 |
VEuPathDBi | HostDB:ENSMUSG00000064061 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055899 | 1 – 1204 | E3 ubiquitin-protein ligase DZIP3Add BLAST | 1204 |
Proteomic databases
EPDi | Q7TPV2 |
MaxQBi | Q7TPV2 |
PaxDbi | Q7TPV2 |
PRIDEi | Q7TPV2 |
ProteomicsDBi | 277659 [Q7TPV2-1] 277660 [Q7TPV2-2] 277661 [Q7TPV2-3] |
PTM databases
iPTMneti | Q7TPV2 |
PhosphoSitePlusi | Q7TPV2 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000064061, Expressed in superior cervical ganglion and 262 other tissues |
ExpressionAtlasi | Q7TPV2, baseline and differential |
Genevisiblei | Q7TPV2, MM |
Interactioni
Subunit structurei
Probably interacts with DAZL.
By similarityGO - Molecular functioni
- phosphatase binding Source: UniProtKB
- polyubiquitin modification-dependent protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 230255, 15 interactors |
IntActi | Q7TPV2, 2 interactors |
MINTi | Q7TPV2 |
STRINGi | 10090.ENSMUSP00000113344 |
Miscellaneous databases
RNActi | Q7TPV2, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 22 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 640 – 681 | DisorderedSequence analysisAdd BLAST | 42 | |
Regioni | 1088 – 1141 | DisorderedSequence analysisAdd BLAST | 54 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 746 – 861 | Sequence analysisAdd BLAST | 116 | |
Coiled coili | 906 – 941 | Sequence analysisAdd BLAST | 36 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1098 – 1124 | Polar residuesSequence analysisAdd BLAST | 27 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1144 – 1184 | RING-type; atypicalPROSITE-ProRule annotationAdd BLAST | 41 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0800, Eukaryota |
GeneTreei | ENSGT00940000161692 |
InParanoidi | Q7TPV2 |
PhylomeDBi | Q7TPV2 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR033103, DZIP3 IPR041249, HEPN_DZIP3 IPR043866, TTC3/DZIP3_dom IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR15727:SF4, PTHR15727:SF4, 1 hit |
Pfami | View protein in Pfam PF19179, DUF5861, 1 hit PF18738, HEPN_DZIP3, 1 hit PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDSLAEEFFV SGNPDVEEQT KEETEIIAEK PVTQLDKQKM DISADPEPVN
60 70 80 90 100
ALLEIKKVLN PISALPKGVF PNIEKFIQED FSFQTMQREV TTHSQTGEEI
110 120 130 140 150
VPALTLHFLI TQLEMALRNI QASNYTAQQI NVGYYLTLLF LYGVALTERA
160 170 180 190 200
KKEDCIEAEN KFLVMKMVIQ ESEICENFMC LVYFGRGLLR CAQKRYNGAL
210 220 230 240 250
LEFYKSLQEI GDTDDNWFEV DPTDDEDLPT TFKDSLNNFI KTTESNIMKE
260 270 280 290 300
TICSYLDCER SCEADILKNT NYKGFFQLMC SKSCCIYFHK ICWKKFKNLK
310 320 330 340 350
YPGESDQSFS GQKCLKEGCP GDMVRMLQCD VPGIVKILFE VVRKDEYITI
360 370 380 390 400
ENLGASYKNL MSLELTDTDI RPKFNLKPNT KDEVPIFKLD YNYFYHLLHI
410 420 430 440 450
IIISGTDMVR QIFDEAMPPT LLKKELLIHK NVLEPYYNHL WTNHPLGGSW
460 470 480 490 500
HLLYPPNKEL PQSKQFDLCL LLALIKHLNV FPAPRKGWDM EPPSSDLSKS
510 520 530 540 550
ADILRLCKYR DILLSEILMN GLTELQFNSI WKKVSDILLR LGMKQDDLDK
560 570 580 590 600
VKENPIENIS LDYHQLSIYL GIPVPEIIQR MLSCYQQGIT LQSITGSQRL
610 620 630 640 650
DVEEFQNDEE DLSPPVMEYN IDVKSNTEIQ LAEINKDVAS IPSESSTESV
660 670 680 690 700
KDLQEVKSKT KKKKRTKSNK KDKDSEDEQV SYMVEKDDQL ETEQVDVNTL
710 720 730 740 750
STYMKTDTSD AQEDSAAEDK FCSLDELHIL DMVEQGSSGK ESTDFKETEK
760 770 780 790 800
ERLAHQHQLY KLQYECEDYK RQLKTVTFRW QENQMLIKKK EKIIVSLNQQ
810 820 830 840 850
VAFGINKMSK LQRQIHAKDD EIKNLKDQLS LKRSQWEMEK HNLESTVKTY
860 870 880 890 900
LNKLNAETSR ALTAEVYFLQ CRRDFGLLHL EQTEKECLNQ LARVTHMAAS
910 920 930 940 950
NLESLQLKAA VDSWNAIVAD VRNKIAFLRT QYNEQINKVK QGFALSTLPP
960 970 980 990 1000
VQLPPPPPSP EILIQQFLGR PLVKESFFRP ILTVPQMPAV CPGVISAAVQ
1010 1020 1030 1040 1050
PRPPLMPGIT WAMPTPIGDT VSPSASLCSE PLMINWERIT DRLKTAFPQQ
1060 1070 1080 1090 1100
TRKELTDFLQ QLKDSHGKSV SRLTFDEIVY KISQMIEPKK SESEEKSAQD
1110 1120 1130 1140 1150
GNNASPSHTA SQPNAPQDPK SAQGSATWEG DKDMDNEEEE EEPCVICHEN
1160 1170 1180 1190 1200
LSPENLSVLP CAHKFHSQCI RPWLMQQGTC PTCRLHVLQP EEFPGHPNGQ
LPKI
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9QNZ2 | E9QNZ2_MOUSE | RING-type E3 ubiquitin transferase | Dzip3 | 1,204 | Annotation score: | ||
F6Z1P8 | F6Z1P8_MOUSE | E3 ubiquitin-protein ligase DZIP3 | Dzip3 | 167 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 306 | D → G in AAH52893 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 650 | V → I in AAH52893 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_010973 | 1 – 889 | Missing in isoform 3. 1 PublicationAdd BLAST | 889 | |
Alternative sequenceiVSP_010977 | 382 – 587 | Missing in isoform 2. 1 PublicationAdd BLAST | 206 | |
Alternative sequenceiVSP_010974 | 890 – 899 | QLARVTHMAA → MFPLCLLFYI in isoform 3. 1 Publication | 10 | |
Alternative sequenceiVSP_010975 | 1135 – 1153 | DNEEE…ENLSP → VRPNLLTVNTFRSERKRMV in isoform 3. 1 PublicationAdd BLAST | 19 | |
Alternative sequenceiVSP_010976 | 1154 – 1204 | Missing in isoform 3. 1 PublicationAdd BLAST | 51 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK122344 mRNA Translation: BAC65626.1 Different initiation. BC052893 mRNA Translation: AAH52893.1 BC117953 mRNA Translation: AAI17954.1 BC117954 mRNA Translation: AAI17955.1 AK039172 mRNA Translation: BAC30265.1 BI736207 mRNA No translation available. CN535823 mRNA No translation available. BG070132 mRNA No translation available. |
CCDSi | CCDS37353.1 [Q7TPV2-2] CCDS49866.1 [Q7TPV2-1] |
RefSeqi | NP_081617.1, NM_027341.2 [Q7TPV2-2] |
Genome annotation databases
Ensembli | ENSMUST00000114516; ENSMUSP00000110161; ENSMUSG00000064061 [Q7TPV2-2] |
GeneIDi | 224170 |
KEGGi | mmu:224170 |
UCSCi | uc007zjy.1, mouse [Q7TPV2-3] uc007zjz.2, mouse [Q7TPV2-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK122344 mRNA Translation: BAC65626.1 Different initiation. BC052893 mRNA Translation: AAH52893.1 BC117953 mRNA Translation: AAI17954.1 BC117954 mRNA Translation: AAI17955.1 AK039172 mRNA Translation: BAC30265.1 BI736207 mRNA No translation available. CN535823 mRNA No translation available. BG070132 mRNA No translation available. |
CCDSi | CCDS37353.1 [Q7TPV2-2] CCDS49866.1 [Q7TPV2-1] |
RefSeqi | NP_081617.1, NM_027341.2 [Q7TPV2-2] |
3D structure databases
AlphaFoldDBi | Q7TPV2 |
SMRi | Q7TPV2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230255, 15 interactors |
IntActi | Q7TPV2, 2 interactors |
MINTi | Q7TPV2 |
STRINGi | 10090.ENSMUSP00000113344 |
PTM databases
iPTMneti | Q7TPV2 |
PhosphoSitePlusi | Q7TPV2 |
Proteomic databases
EPDi | Q7TPV2 |
MaxQBi | Q7TPV2 |
PaxDbi | Q7TPV2 |
PRIDEi | Q7TPV2 |
ProteomicsDBi | 277659 [Q7TPV2-1] 277660 [Q7TPV2-2] 277661 [Q7TPV2-3] |
Protocols and materials databases
Antibodypediai | 32388, 163 antibodies from 21 providers |
Genome annotation databases
Ensembli | ENSMUST00000114516; ENSMUSP00000110161; ENSMUSG00000064061 [Q7TPV2-2] |
GeneIDi | 224170 |
KEGGi | mmu:224170 |
UCSCi | uc007zjy.1, mouse [Q7TPV2-3] uc007zjz.2, mouse [Q7TPV2-2] |
Organism-specific databases
CTDi | 9666 |
MGIi | MGI:1917433, Dzip3 |
VEuPathDBi | HostDB:ENSMUSG00000064061 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG0800, Eukaryota |
GeneTreei | ENSGT00940000161692 |
InParanoidi | Q7TPV2 |
PhylomeDBi | Q7TPV2 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 224170, 4 hits in 72 CRISPR screens |
ChiTaRSi | Dzip3, mouse |
PROi | PR:Q7TPV2 |
RNActi | Q7TPV2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000064061, Expressed in superior cervical ganglion and 262 other tissues |
ExpressionAtlasi | Q7TPV2, baseline and differential |
Genevisiblei | Q7TPV2, MM |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR033103, DZIP3 IPR041249, HEPN_DZIP3 IPR043866, TTC3/DZIP3_dom IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR15727:SF4, PTHR15727:SF4, 1 hit |
Pfami | View protein in Pfam PF19179, DUF5861, 1 hit PF18738, HEPN_DZIP3, 1 hit PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DZIP3_MOUSE | |
Accessioni | Q7TPV2Primary (citable) accession number: Q7TPV2 Secondary accession number(s): Q148V3, Q80TU4, Q8BYK7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | July 19, 2004 | |
Last modified: | May 25, 2022 | |
This is version 150 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways